qiong zhang lina 2000 isolation and structural analysis of polysaccharides from poria cocos mycelia the chinese university of hong kong hong kong

Tài liệu Báo cáo Y học: Puriﬁcation, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

Ngày tải lên : 22/02/2014, 07:20

... at their 50 and 30 boundaries, respectively, and were inserted between the third letter of one codon and the ﬁrst letter of the following codon Molecular modeling of the C sepium b-amylase The ... the X-ray coordinates of the soybean b-amylase (Fig 4) According to the Ramachandran plot of this model the f and c angles of most of the residues are in the allowed regions of low energy, except ... According to the results of previously reported molecular and structural studies the processing of the b-amylases from sweet potato [3,38] and soybean [39,40] also is restricted to the removal of the...

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Báo cáo khoa học: Puriﬁcation and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

Ngày tải lên : 07/03/2014, 12:20

... sequence and sequence alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the ﬁgure indicate the positions of the nucleotide sequence The ... in the pollen As a consequence, individuals allergic to BGP require individual diagnosis and therapy, and an understanding of the structure of these allergens is essential to the improvement of ... Promotion of Research-oriented university program from the Ministry of Education and Grant NSC 92–2320-B-002174 from the National Science Council, Taiwan (LPC) and VGH-93–302 from the Taipei...

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Báo cáo Y học: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases pot

Ngày tải lên : 08/03/2014, 23:20

... phylogenetic analysis the separate clustering within the tpsb family of C62 and M34 from B93 and D85 is clear (Fig 2) The B93 and D85 sequences group together with the myrcene synthase from Q ilex and the ... terpinolene, linalool and a-terpineol cDNA isolation and sequencing Random sequencing of a cDNA library made from mRNA isolated from the peel of young lemon fruits resulted in the identiﬁcation of nine ... products of the monoterpene synthases isolated and characterized in the present paper Only the origin of the trace amounts of linalool, a-terpineol and (E)-b-ocimene that are also present in the lemon...

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Báo cáo khóa học: Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding pdf

Ngày tải lên : 16/03/2014, 16:20

... that of the wild-type enzyme (data not shown) On the other hand, the Km value for benzonitrile of the aT109S mutant was similar to that of the wild-type enzyme (Table 2) The binding affinity of ... NotI and HindIII The PCR product was ligated to a pUC18 plasmid The resulting plasmid was then sequenced to confirm the presence of the mutation Expression and purification of each mutant and the ... programs CNS [25] and XFIT [26] At the final stage of refinement of the complex structure, n-butyric acid was added to the model according to the Fo–Fc map At the final stage of refinement of the aY114T...

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Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis pptx

Ngày tải lên : 17/03/2014, 17:20

... cloning of the gene encoding the secreted CM from Yersinia pestis (*YpCM, y2828), puriﬁcation of the protein, investigation of the properties of the enzyme, and the crystal structure analysis of the ... form, and the rest of the structure is in cartoon form In EcCM, one polypeptide chain is gray and the other is rose In 90-MtCM, one polypeptide chain is blue and the other is green The TSA from the ... tail of one protomer to almost reach the active site of the neighbor Active site of 90-MtCM The structure of EcCM includes the TSA, which clearly identiﬁes the active site From structural and...

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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx

Ngày tải lên : 23/03/2014, 13:20

... et al of the ndh genes is much higher in BS chloroplasts, and elevated amounts of the Ndh complex have been found in these plastids [18] The function of the Ndh complex is still a matter of debate ... about the viability of ndh mutants [2,23,25] have clearly restarted the debate about the real function(s) of the Ndh complex To contribute to the structural and functional characterization of this ... characterize the Ndh complex, the MS and BS chloroplasts were separated on CN ⁄ PAGE (1D, based on the internal charge of the protein complexes) and BN ⁄ PAGE (2D, based on the external charge of the...

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báo cáo khoa học: " Characterization and structural analysis of wild type and a non-abscission mutant at the development funiculus (Def) locus in Pisum sativum L" pdf

Ngày tải lên : 12/08/2014, 03:20

... Arrangement of pea seeds to the replum in a pod of the wild type pea (C) Inseparable attachment of the seed to the funicle in the mutant pea The intervening space which delimits the funicle from the ... controlling the abscission event of pea seeds Absence of the hilum palisade layers is the key characteristic in the def mutant pea seed Structural analysis revealed the absence of the palisade layer and ... to the growing of the plants, harvested materials, carried out the structural examination and drafted the manuscript YKL participated in designing the experiments, structural analysis and the...

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Báo cáo y học: "A systematic comparative and structural analysis of protein phosphorylation sites based on the mtcPTM database" ppt

Ngày tải lên : 14/08/2014, 07:21

... compromising the structural integrity of the core domain On the other hand, when the modified residues are part of the hydrophobic core of the domain, the modification could cause considerable structural ... Phosphorylation of T432 may affect the relative orientations between domains within the protein, whereas Y29 may modulate the conformation of the helical subdomain The orientations of the domains and of the ... similar characteristics of serine and threonine, and the fact that they are usually targeted by the same protein kinases The study focused on the following structural features of the phosphosites:...

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Báo cáo y học: "Sequence and structural analysis of BTB domain proteins" pps

Ngày tải lên : 14/08/2014, 14:22

... formed between β1 from one monomer and β5 of the other monomer; and the packing of α1 from one monomer against α1 and the A1/A2 helical hairpin from the other monomer The strand-exchanged amino ... BTB-ZF and BBK proteins in the vertebrates; the MATH-BTB proteins in the worm; the BTB-NPH3 proteins in the plant; the Skp1 proteins in the plant and worm; and the T1 proteins in worm and vertebrates ... between this region and the co-repressor [5] Mutation of Asp35 and Arg49 disrupts the proper folding of PLZF [6], and these residues are thus important for the structural integrity of the domain Interestingly,...

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Báo cáo y học: "Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins" doc

Ngày tải lên : 14/08/2014, 16:21

... alignments of the (a) Mis12Mtw1 and (b) Ndc80Hec1 families Schematic drawing above the alignment indicate the length of the S cerevisiae proteins and the percentages denote the degree of similarity of ... Symptomatic of the simplicity of the E cuniculi kinetochore is the absence of the vast majority of potential MAPs Nonetheless, it is significant that the E cuniculi kinetochore contains three of the ... and anaphase [1]; and they generate the spindle checkpoint signals linking anaphase onset to the completion of kinetochore-MT attachment [2] Despite the conservation of these functions, and of...

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Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

Ngày tải lên : 07/03/2014, 16:20

... to the cytosol Here we conﬁrm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p ... of Pex1p and Pex6p A B Fig Two-hybrid interaction of Pex1p and Pex6p harboring point mutations of the WalkerA and B motifs of their ATP-binding sites A schematic representation of the Pex1p and ... the binding sites of these two AAA-proteins to their different protein regions and elucidated the importance of ATP-binding to the two AAA-cassettes of Pex1p and Pex6p for their interaction The...

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Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot

Ngày tải lên : 08/03/2014, 09:20

... core-oligosaccharides of the R1 and R2 core-types [8] whereas the chemical structure of the inner core-region of the E coli R4 core-type was hitherto unknown The chemical structure of the outer core region of the ... between the anomeric proton of the side-chain heptose (residue H) and the axial H-3 of the ﬁrst aKdop (residue C) has been observed in the analysis of the R1 and R2 core oligosaccharides [8] and ... assignment of H-7a/b and C-7 of L,D-Hepp residues F and H The chemical shift of H-6 of residue F could also be assigned in the latter spectrum The chemical shift of the H-6 proton of residue...

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Báo cáo khoa học: Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway – evidence of a different substrate speciﬁcity doc

Ngày tải lên : 16/03/2014, 00:20

... shows 33% identity and 51% similarity to the TenA protein from B subtilis The 3D structure of the monomer is quite similar to that of the other members of the TenA family of known structure: ... The superposition of the Ca atoms of one monomer with that of the other members ˚ of the family gives a rmsd of 1.7, 1.3 and 1.4 A for models 1RTW, 1UDD and 1TYH, respectively The major differences ... Cys135 adds to C6 of the pyrimidine ring, favoring the exit of the aminic group The subsequent addition of a water molecule and the expulsion of the active cysteine complete the reaction Asp44...

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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx

Ngày tải lên : 17/03/2014, 03:20

... upstream from the poly(A) of GT-cDNA1 and another is located 11 bp upstream from the poly(A) of GT-cDNA2 (data not shown) Of particular interest is the presence of eight AUUUA motifs in the 3¢-UTR of ... Organization of the gcGLUT gene (A) The 12 exons of the gcGLUT gene are shown as boxes Filled and open boxes indicate translated and untranslated regions, respectively The position of the start (ATG) and ... strong structural homology with six of the respective exons in the hGLUT1 and hGLUT3 Ó FEBS 2003 3016 Z Zhang et al (Eur J Biochem 270) genes, and four of the respective exons in hGLUT2 and hGLUT4...

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Báo cáo khoa học: Structural and functional analysis of ataxin-2 and ataxin-3 potx

Ngày tải lên : 30/03/2014, 15:20

... exposed N-termini of the second and fourth b-strand in the crystal structure of the UBX domain of the cofactor p47 (PDB identiﬁer 1s3s) [86] Similar to the P falciparum homologue, the Cryptosporidium ... part) The known DSSP secondary structure assignment of the Sm1 protein from P abyssi is shown at the top of the alignment (cylinder for a-helix, arrow for b-strand), and the amino acid sequences of ... guanidinium groups of arginines R69/V335 of D3 and R25/G287 and R49/T312 of B as well as an ionic interaction between E21/Q282 of D3 and R65/S322 of B stabilize the dimer further However, the latter...

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MECHANICAL AND METALLURGICAL ANALYSIS OF STRUCTURAL STEEL pot

Ngày tải lên : 01/04/2014, 00:20

... overview of the results of the mechanical and metallurgical analysis of structural steel from the World Trade Center (WTC), part of the National Institute of Standards and Technology Investigation of ... overview of the mechanical and metallurgical analysis of structural steel from the WTC, part of the National Institute of Standards and Technology (NIST) Investigation of the WTC disaster The purpose ... of failure of certain parts of the flooring in this area of the tower At the moment of collapse of WTC 2, the top portion of the building was found to have moved to the west as it tilted to the...

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Báo cáo y học: " Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx

Ngày tải lên : 11/08/2014, 21:21

... against the other side of the b clamp, resulting in ring opening For the T4 system, interaction with DNA and the presence of the gp43 DNA Page of 17 Figure Structures of T4 gp45 clamp and the E coli ... protein and gp61 primase likely coordinates the handoff of the RNA primer to the gp43 DNA polymerase, establishing a synergy between leading strand progression and lagging strand synthesis [84] The ... of the ten full-length T4 replisomal proteins have been determined; the gp59 helicase loading protein, the RNase H, and the gp45 processivity clamp The core of T4 gp32 and two proteins from the...

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báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc

Ngày tải lên : 12/08/2014, 03:20

... absorbed and the rest inundated the soil After 24 h of the initial NaCl treatment, the leaves of the seedlings were harvested, and were preserved in liquid N2 until further analysis The leaves from the ... picked-up The results of the differential screening of the EST clones from both the forward and the reverse SSH cDNA libraries are shown in Fig Most of the 150 spotted plasmids from the randomly ... activity study The effect of NaCl on the activity of catalase was also studied by in-gel activity staining of the enzyme activity The enzyme extracts from the leaves of the control and NaCl treated...

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Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 1

Ngày tải lên : 09/09/2015, 10:20

... introduce some of the members of the basic-helix-loop-helix (bHLH) family of transcription factors and then focus on a special group of HLHcontaining proteins that antagonizes the function of these bHLH ... homodimerize on the E-box motif (CANNTG) of enhancer regions of a number of genes to activate their transcription One such example was the enhancer region of the heavy and light chain of the immunoglobulin ... heterodimer of ID3 with E37, the structures were structurally aligned and a monomer from each removed to reveal the much shorter helix of ID3 at the N-terminal end where the basic domain of E47 would...

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Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 2

Ngày tải lên : 09/09/2015, 10:20

... experiments used LB media and were induced with 0.2 mM IPTG The order of the samples was the same for all gels apart from the positions of the marker Red boxes denote where expected bands should be (A) ... Hence, the specific aims of this study were to: 1) Clone, express, purify and crystallize ID2 2) Solve the crystal structure of ID2 3) Analyze the structure of ID2 and look at similarities and differences ... (TCF3) and MyoD (MYOD1) but very little was known about why they were so specific in their interactions given the structural similarities to each other and to all the different groups of HLH-containing...

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