pyruvate glucose assay patient samples
Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... plays an important role in glucose homeostasis and has shown promising effects as a new treatment for type diabetic patients [3] The main function of GLP-1 is to enhance glucose- dependent insulin ... enterokinase cleavage Glucose- lowering effect of gAd-GLP-1-A fusion protein We investigated the glucose- lowering effect of gAd-GLP-1-A fusion protein in diabetic mice Blood glucose was respectively ... gAd-GLP-1-A fusion protein The digested products were analyzed by SDS-PAGE and Western blot analysis Assay of glucose- lowering effect of gAd-GLP-1-A fusion protein Male KM mice were housed at 23-25°C in...
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Báo cáo khoa học: A novel, promoter-based, target-specific assay identifies 2-deoxy-D-glucose as an inhibitor of globotriaosylceramide biosynthesis docx
... untreated control; 2, 10 mM 2DG; 3, 30 mM glucose (high glucose treatment); 4, 10 mM streptozotocin; 5, 200 nM mithramycin A; 6, 100 lM citrate; 7, 10 mM pyruvate; 8, 50 lM splitomicin; 9, 100 lM ... NCCIT cells 2DG, a nonmetabolizable glucose analogue, acts as a glycolytic inhibitor by inhibiting glucose hexokinase Thus, 2DG has frequently been used as a glucose starvation mimetic Recently, ... study, we developed a simple and convenient assay for monitoring Gb3S promoter activity by using a secreted luciferase reporter gene Because this assay is able to measure Gb3S promoter activity...
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Báo cáo sinh học: "A large, consistent plasma proteomics data set from prospectively collected breast cancer patient and healthy volunteer samples" doc
... volunteers and volunteer breast cancer patients scheduled to begin a new treatment regime (here designated as ‘baseline’ samples) These patients also provided samples at each month follow up visit ... breast cancer patient volunteer plasma samples, we completed LCMS proteomic profiling analyses on approximately 400 follow up samples collected every three months from the breast cancer patient volunteers ... profiling platform is required for proteomic analyses of plasma samples donated by healthy volunteers and breast cancer patients We collected samples in a clinical trial-like protocol as part of an NCI-sponsored...
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Báo cáo khoa học: " A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples" potx
... biosensor assay was compared with the microtiter plate assay employing the same probe and target sequences for the detection of RNA extracted from fecal samples The microtiter plate assay was ... organisms [19] Hence, we used fecal samples in this assay The lateral flow biosensor assay was performed with the RT-PCR product of RNA extracted from spiked fecal samples containing 10 to 10 organisms ... fecal samples, we also tested fecal samples from experimentally infected animals, wherein fecal samples collected and days post-challenge gave positive results by the lateral flow biosensor assay...
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Báo cáo y học: " Analysis of the copy number profiles of several tumor samples from the same patient reveals the successive steps in tumorigenesis" ppt
... tumor samples from patients admitted between 1993 and 2002 to the Henri Mondor Hospital Thirteen of these samples were multiple tumors from five patients (two to four metachronous or synchronous samples ... bladder carcinogenesis Five patients for whom two to four bladder cancer samples were available were analyzed with the TuMult algorithm In four cases, we had metachronous samples obtained at different ... samples taken from different anatomic sites in 14 patients, are available from the Gene Expression Omnibus database [32] We used these data to reconstruct the tumor progression tree in each patient...
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Báo cáo y học: "Tight blood glucose control: a recommendation applicable to any critically ill patient" ppsx
... TW: Glucose control and mortality in critically ill patients JAMA 2003, 290:2041-2047 Krinsley JS: Effect of an intensive glucose management protocol on the mortality of critically ill adult patients ... tight glucose control with intensive insulin in these patients, but there is uncertainty regarding whether tight glucose control is beneficial in patients who have not undergone cardiac surgery Fear ... days Insulin was administered to only 9% of the patients during the 48-hour period of observation In addition, patients received a mean of only 22.5 g glucose/ day, and were fed early after surgery...
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Báo cáo y học: " Recently published papers: Sepsis, glucose control and patient–doctor relationship" pdf
... 4.12 mmol/l Blood glucose differences > 5.56 mmol/l were excluded Their results showed a poor correlation between venous and fingerprick testing of blood glucose In 44 paired samples (83%) the ... This prospective observational study enrolled 80 patients with Acute Physiology and Chronic Health Evaluation (APACHE) II scores of 15 ± Simultaneous samples were taken once per day Accuracy was ... views of patients and relatives with experience of the ICU regarding what attitudes and skills they expect specialists in critical care to have A structured questionnaire was sent to patients...
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Báo cáo y học: " Formulas Pro/con debate: Is intensive insulin therapy targeting tight blood glucose control of benefit in critically ill patient" doc
... insulin therapy to maintain tight blood glucose control in our patients? Our clinical practice is in a multidisciplinary adult ICU that admits medical patients and patients following general surgery, ... authors, the patients studied in that trial were ventilated surgical patients admitted to the ICU after predominantly cardiac surgery and these results cannot be extrapolated to other ICU patient ... SICU patients The beneficial effect seems to be even more pronounced in the most severely ill patients requiring prolonged intensive care The In both Van den Berghe and colleagues’ studies, the patients...
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Báo cáo y học: " Formulas Patient-level glucose reporting: averages, episodes, or something in between" pdf
... independent predictor of mortality [8] Among groups of patients having myocardial infarction or congestive heart failure, observational patient- level data suggest that when mortality is considered ... infusion reduces mortality in patients with diabetes undergoing coronary artery bypass grafting J Thorac Cardiovasc Surg 2003, 125:10071021 Krinsley JS: Effect of an intensive glucose management protocol ... adult patients Mayo Clin Proc 2004, 79:992-1000 Kagansky N, Levy S, Rimon E, Cojocaru L, Fridman A, Ozer Z, Knobler H: Hypoglycemia as a predictor of mortality in hospitalized elderly patients...
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THE ROLE OF PYRUVATE DEHYDROGENASE KINASE IN GLUCOSE AND KETONE BODY METABOLISM
... glycogen from glucose Insulin stimulated glycogen synthesis and glucose uptake by GLUT4 removes glucose from the circulation and reduces blood glucose levels to normal 1.2 Regulation of blood glucose ... existing glucose The glucose production rate (GP) is determined by the following equation: GP (mg kg-1min-1) = [U-13C6] glucose infusion rate (mg kg-1min-1) Ep (mg [U-13C6] glucose/ mg glucose) ... C glucose (μmol/min) (outflow) C glucose (μmol/min) 13 12 gives 13C glucose (μmol)/12C glucose (μmol) which corresponds to the isotopic equilibrium (Ep) We can substitute Ep for outflow: C glucose...
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Development of a miniaturization assay platform and its application to study scarce biological samples
... (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, the MTS tetrazolium assay, the AlamarBlue® assay and the Promega series of screening assays, including Caspase and assays [26–29] The AlamarBlue® assay is a popular ... abundance Assay • Compatible to HTS platform • Convenient to miniaturize • Duration of assay Samples • Biologically relevant to disease type • Derive in limited supply from patients Assay • Robust ... Relevant Assays for Drug Discovery In the 21st century, there has been a growing trend to move away from biochemical-based assays, and to apply cell-based assays for drug discovery [34] Cell-based assays...
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Báo cáo y học: "Electronic patient record use during ward rounds: a qualitative study of interaction between medical staff"
... one Figure Paper patient medical record Paper patient medical record 1, binder; 2, drug chart; 3, patient plan of the day; 4, observation chart; 5, personal notes Electronic patient record The ... bed to bed each morning to review patient progress The team updates itself on each patient' s condition through discussion and chart review, and decides upon the patient' s plan for the day As the ... Morrison et al Figure Electronic patient record summary screen The summary screen, displayed on a 19-inchscreen at the patient bedside, contains the most imporElectronic patient record summary screen...
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Báo cáo y học: " Patient Specification Quality Assurance for Glioblastoma Multiforme Brain Tumors Treated with Intensity Modulated Radiation Therapy"
... multileaf collimator (MLC) QA, the measurements of individual patient fluence maps, the calibration of the tools used, and the reproducibility of patient positioning [18] The planned dose fluence is compared ... for IMRT plans that are going to be used to treat patients with GBM brain tumors Presently, we perform routine QA measurements for each IMRT patient either immediately prior to the treatment ... VeriSoft software measured dose, and the % of pixels passing gamma criterion for the 10 patient treatment plans Patient s fields numbers Fraction Planned Dose, cGy 11 11 11 IMRT fields total of =...
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Báo cáo y học: "ISOLATION OF CHLAMYDIA PNEUMONIAE FROM SERUM SAMPLES OF THE PATIENTS WITH ACUTE CORONARY SYNDROME"
... in: Serological assay IgA IgG TaqMan PCR in serum Bacteriological assay with further PCR validation PRELIMINARY GROUP Patients with ACS 18 MAYOR GROUPS Healthy volunteers 26 - Patients with ACS ... isolated from the serum samples of the patients with acute coronary syndrome Furthermore, using combination of bacteriological and PCR-based methods we show herein that patients with acute coronary ... molecular size standards; 2, and – PCR positive serum from patient M.; and – PCR positive serum from patient P.; and 10 – PCR negative serum from patient S.; and 11 - extraction control; and 12 – negative...
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Báo cáo y học: " WT1 PEPTIDE VACCINATION IN COMBINATION WITH IMATINIB THERAPY FOR A PATIENT WITH CML IN THE CHRONIC PHASE"
... Biosciences) Cytotoxicity assay To estimate anti-WT1 cytotoxicity of MLPC cells, a 5,6-carboxy-fluorescein succinimidyl ester (CFSE; Molecular Probes, Eugene, OR)-based cytotoxicity assay was performed ... experienced r relapse of the disease e They s showed that patient had cytogenetic CR and d c http://www.m medsci.org Int J Med Sci 2010, three patients refractory to allogeneic transplantation, interferon ... imatinib therapy, we started WT1 peptide vaccination therapy in combination with imatinib in a CML patient who could not acquire a major molecular response through the administration with a single...
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Báo cáo y học: "The characterisation of mucin in a mature ovarian teratoma occurring in an eight year old patient
... secreted by the right ovary of an eight year old patient with a bilateral ovarian mature teratoma, using both biochemical and histological techniques The Patient Clinical Findings An eight-year-old ... Electrophoresis (SDS-PAGE) Mucin samples were prepared in gel loading buffer containing 2% SDS, 10% glycerol, and 0.01% bromophenol blue 2ul of 5% mercaptoethanol was applied to samples and boiled for ... Western blot was performed to test for reactivity between the polyclonal antibody and the mucin samples Samples were electrophoresed in 1.0% (w/v) agarose gel prepared in 40mM Tris-acetate/1mM Int...
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Điều hòa trao đổi Glucose
... động là: ↑ vận chuyển glucose mô mỡ ↑ Kết nạp a.a ↑ Tổng hợp protein ↓ Phân huỷ protein ↑ Tổng hợp mỡ gan mô mỡ ↓ Phân huỷ mỡ mô mỡ ↑ Chuyển hoá Glucose thành Glucose –↓ Tạo glucose gan – P gan ... Hoàng Nguyễn Công Trình Page 16 Glucose Pyruvate AcetylCoA TCA cycle CO2 H2O ATP Năng lượng Glucose dư insulin xúc tác kết hợp lại với để hình thành glycogen chuyển glucose thành chất béo mô mỡ ... pyruvic, sau thành glucose 3,Đích Glucagon gan, nơi thúc đẩy: -Việc phá vỡ glycogen thành glucose -Tổng hợp Glucose từ Acid Lactic từ phân tử Hydratcarbon acid béo aa -Việc phóng thích glucose vào máu...
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