purification and characterization of carbon monoxide dehydrogenase from the aerobic hyperthermophilic archaeon aeropyrum pernix
Fisheries science JSFS , tập 76, số 2, 2010 3
Ngày tải lên :
19/07/2016, 19:43
... Demonstration of the effect of the fish stock on the species composition of zooplankton and the intensity of metabolism of the whole plankton association Verh Int Ver Theor Gewandte Limnol 14:192–195 ... the control and CaDPD group Student’s t test for paired data was used to test the significance of the means of the calcium and phosphate content of the scales before and after incubation in the ... habits of larvae/fry of C a grandoculis and their impact on the plankton community in rice fields To this, we regularly surveyed the gut contents of C a grandoculis and the community structure of...
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BÁO CÁO " ẢNH HƯỞNG CỦA ĐỘ MẶN THẤP LÊN ĐIỀU HÒA ÁP SUẤT THẨM THẤU + + VÀ HOẠT TÍNH MEN NA /K ATPASE Ở TÔM THẺ CHÂN TRẮNG (Litopenaeu s vannamei) " pptx
Ngày tải lên :
02/04/2014, 10:20
... immunoreactivity in the blue crab: Tissue distribution, variations in the molt cycle, and partial characterization J Exp Zool 262:279-286 Castille Jr F.L and A.L Lawrence 1981a The effect of salinity on the ... comparison of osmotic, sodium and chloride concentrations between the urine and hemolymph of Penaeus seti ferus (L.) and Penaeus stylirostris stimpson Comp Biochem Physiol 70A:525-528 Chen J.C and J.N ... 0-9708605-1-X Printed in the United States of America Ferraris R.P., F.D Parado-Estepa, E.G de Jesus and J.M Ladja 1987 Osmotic and chloride regulation in the hemolymph of the tiger prawn Penaeus...
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Báo cáo nghiên cứu khoa học: " ẢNH HƯỞNG CỦA ĐỘ MẶN THẤP LÊN ĐIỀU HÒA ÁP SUẤT THẨM THẤU + + VÀ HOẠT TÍNH MEN NA /K ATPASE Ở TÔM THẺ CHÂN TRẮNG (Litopenaeu svannamei)" ppsx
Ngày tải lên :
22/07/2014, 11:21
... immunoreactivity in the blue crab: Tissue distribution, variations in the molt cycle, and partial characterization J Exp Zool 262:279-286 Castille Jr F.L and A.L Lawrence 1981a The effect of salinity on the ... comparison of osmotic, sodium and chloride concentrations between the urine and hemolymph of Penaeus seti ferus (L.) and Penaeus stylirostris stimpson Comp Biochem Physiol 70A:525-528 Chen J.C and J.N ... 0-9708605-1-X Printed in the United States of America Ferraris R.P., F.D Parado-Estepa, E.G de Jesus and J.M Ladja 1987 Osmotic and chloride regulation in the hemolymph of the tiger prawn Penaeus...
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Báo cáo Y học: The DNA-polymerase inhibiting activity of poly(b-L-malic acid) in nuclear extract during the cell cycle of Physarum polycephalum pot
Ngày tải lên :
31/03/2014, 21:21
... Therefore, the higher the concentration of free PMLA, and the lower the value of the inhibition constant (Ki), the higher the relative inhibitory activity (vo ) vi)/vi In the presence of ligands ... Materials and methods, the degree of inhibition depends on both the concentration of free PMLA and the inhibition constant This parameter reflected the affinity of the polymerase and both the affinity and ... a and e [13], and DNA polymerase d [14] were consistent with the recovery of > 95% of DNA polymerase a and > 75% of the other DNA polymerases in the extract Standard DNA polymerase activity and...
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effects of magnesiumphosphoruspotassium containing in water on molting, growth and survival rate of the white shrimp (litopenaeus vannamei) juveniles, reared in low salinity water
Ngày tải lên :
22/09/2015, 12:38
... to the diet, but according to the pH of the anterior part of the stomach differences are expected in relation to the nature of the phosphate salt (Velasco et al., 2001) As a component of these ... most of their body content of minerals during molt and replace it from the water White shrimp live in an environment that is hypertonic and continually drink small amounts of water and thus they ... until the next molting cycle Duration of the molting cycle depends on the species and age of the shrimp Molting frequency on white shrimp decreased along with increasing the size of shrimp The...
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Tài liệu Báo cáo khoa học: The phosphatase activity of the isolated H4-H5 loop of Na+/K+ ATPase resides outside its ATP binding site docx
Ngày tải lên :
19/02/2014, 16:20
... surface of the N-domain is due the formation of an hydrogen bond of the NO2-group of pNPP with the amino group of N398 The hydrophobicity of the binding pocket is achieved by W411 and L414 The phosphate ... experiments, the position of the binding site for ATP at the front side of the N-domain between I390 and L576 and reveals the separate existence of a p-nitrophenylphosphatase at the rear site of the N-domain ... truncation of the (L354I604) GST fusion proteins of the H4-H5 loop on the activities and properties of the p-nitrophenylphosphatase activity was studied The properties of the control refer to the mean...
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Báo cáo khoa học: The molecular chaperone a-crystallin incorporated into red cell ghosts protects membrane Na/K-ATPase against glycation and oxidative stress ppt
Ngày tải lên :
17/03/2014, 03:20
... Ashcroft, at the Diabetes Research Laboratories, University of Oxford for the use of his luminometer; and to Dr Simon Golding at the Department of Physiology, University of Oxford for the use of the ... inactivation of the enzyme by the modifier is due to the targeting of a domain of the enzyme that is accessible to both the modifier and to intracellular a-crystallin The cytoplasmic loop of Na/K-ATPase ... significant to the overall amount of fructose in the cell The modifiers can all pass through the membrane so a steady state would be achieved at the molarity of the modifying agent The site of modification...
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Báo cáo Y học: High pressure-induced changes of biological membrane Study on the membrane-bound Na+/K+-ATPase as a model system pdf
Ngày tải lên :
24/03/2014, 00:21
... methanol and chloroform in a test tube and the solvent was then evaporated with nitrogen gas ¯ushing The ®lm of soy-PtdCho which formed on the inner wall of the test tube was mixed with mL of 50 ... protein, and/ or the destruction of integral structure (lipid bilayer and protein) The issue of clarifying the precise nature of the interrelationship between these pressure-induced changes and enzyme ... in the thickness of the lipid bilayer and a decrease in the cross-sectional area, due to the ordering of hydrocarbon chains [43±50] For example, the thickness of a bilayer which is composed of...
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Na+, k+ ATPase in single skeletal muscle fibres and the effects of ageing, training and inactivity
Ngày tải lên :
04/12/2015, 13:59
... IIa, IIa/x or IIx) The MHC isoform present will dictate the speed that the fibre contracts and thereby the rate of cross bridging cycling and maximal shortening velocity of the fibre In human ... glycosylated and is necessary for the structural maturation of the α subunit, localisation of the NKA heterodimer to the sarcolemma and regulation of NKA activity 35 (Cougnon et al., 2002) These NKA isoforms ... functions of each isoform, as a knockout of either of the three α isoforms leads to the death of rats and mice before or immediately following birth (Lingrel et al., 2007) Research using isoform...
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Báo cáo khoa học: Stimulation of p-nitrophenylphosphatase activity of Na+ ⁄ K+-ATPase by NaCl with oligomycin or ATP docx
Ngày tải lên :
07/03/2014, 16:20
... pNPPase activity and Rb+ binding (Fig 4A,B) In the presence of oligomycin, the binding curve of Rb+ and the activation curve of pNPPase showed a convex shape The peak of both Rb+ binding and pNPPase ... Homareda and M Ushimaru Stimulation of pNPPase activity of Na+ ⁄ K+-ATPase the E1 and E2 states coexist depending on the ligand conditions (Fig 11) The consistency between the proposed model and the ... regarded as the occluded Na+ [13], was calculated from the difference between the radioactivities of the pellets in the presence and absence of oligomycin H Homareda and M Ushimaru the difference...
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Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
Ngày tải lên :
16/03/2014, 13:20
... gradually in the nucleus during phases S and G2, and was detected maximally in the cytoplasm in the M phase The timing of phosphorylation and dephosphorylation of MCM4 during phases G2 and M was ... the bottom Antibodies used and temperature for culture are indicated at the top In the bottom, the ratio (39 ⁄ 33 °C) of the intensity of the signals is shown (B) A dominant-negative mutant of ... HeLa cells The effect of the expressed CDK on the phosphorylation of MCM4 was examined by costaining with anti-HA and antiphosphoMCM4 sera (P-3 or P-32) Each of the single stainings and their merged...
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Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
Ngày tải lên :
23/03/2014, 06:20
... analyzed the expression profiles of MLL1, CGBP, Ash2 and Rbbp5, together with cyclins E and B, as a function of the cell cycle Western blot analysis of the whole-cell extract and histones from different ... attenuated for the rest of the cell cycle (10 and 20 h), correlating with the expression of the gene (Fig 6C,D) The correlation of promoter occupancy of MLL1, H3K4 trimethylation and RNAP II with ... In the case of the HoxA10 gene, significantly higher levels of RNAP II and MLL1 recruitment and H3K4 trimethylation marks were observed at the beginning of the S phase (0 h), and these marks were...
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Báo cáo khoa học: No evidence for a role in signal-transduction of Na+/K+-ATPase interaction with putative endogenous ouabain potx
Ngày tải lên :
23/03/2014, 17:21
... hormone and the adrenal gland the major place of synthesis of ouabain However, it is still much debated whether ouabain or OLF really exist in mammalian blood and tissues, and if they do, whether ... exclusively by the pump The mentioned, often contradictory observations and interpretations not add to the credibility of the hypothesis The main objections to a normal signaltransducing function of ouabain ... in the order of 10 mV to the membrane potential due to the stoichiometry of (3 : 2) Na+/K+-exchange by the pump On the other hand, according to the pump-leak model, the membrane potential is...
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Báo cáo Y học: Na+/K+-ATPase as a signal transducer Zijian Xie and Amir Askari docx
Ngày tải lên :
24/03/2014, 00:21
... growth, and that it is essential to delineate the pathways activated by each signal/receptor, and the nature of crosstalk among these, before the consequences of the pathological derangement of the ... emphasizing the diversity of the signal transducing functions of Na+/K+ATPase (see below) CONCLUSIONS AND FUTURE PROSPECTS The work of the past few years, built on the foundation of a number of excellent ... other cardiac hypertrophic stimuli These findings clearly establish that Na+/K+-ATPase indeed regulates the growth and the phenotype of the cardiac myocyte; and raise the intriguing question of...
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Báo cáo y học: " Distinguishing between linear and exponential cell growth during the division cycle: Single-cell studies, cell-culture studies, and the object of cell-cycle research" ppsx
Ngày tải lên :
13/08/2014, 23:20
... the child would stand against the board and their height would be measured by drawing a line and dating the line At the left is the full board and at the right is a close-up of a portion of the ... random occasions, usually family gatherings When measuring time occurs the grandchildren stand against the board, a line is drawn at the top of their head, and the date of the line is noted The ... all of the growth patterns of the individual components, the growth law would be the weighted sum of these growth patterns As the cytoplasm is by far the major component, the other parts of the...
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Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt
Ngày tải lên :
16/02/2014, 09:20
... levels of apoptotic markers during the late stages of MPyV infection However, comparison of the levels of apoptotic markers and cytotoxicity during the replication of wt MPyV, and replication of the ... projects 1M0508, MSM0021620858 and LC545 from the Ministry of Education, Youth and Sports of the Czech Republic and by project no 156307 from the Grant Agency of the Charles University in Prague ... as a control of sample loadings Discussion In the present work, the cytotoxic properties of the minor structural proteins (VP2 and VP3) of the MPyV were studied in the absence of other virus components...
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Tài liệu Báo cáo khoa học: Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells doc
Ngày tải lên :
18/02/2014, 17:20
... sense and antisense strands of the piRNA hairpin are involved in formation of the piRNA-interacting complex (piRC) and function in the transcriptional gene silencing of retrotransposons and genetic ... positions and sequences used to amplify pri-miR9-1, 9-2 and 9-3 Arrows indicate the position and orientation of the primers Bold font represents the sequence of mature miR9* and italic fonts the sequence ... vector The promoter sizes of the construct are indicated by numbers in parentheses )44 means located 44 bp upstream of the first base pair at the 5¢-end of pre-miR-9-1 defined as +1 FLuc is the ORF of...
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Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc
Ngày tải lên :
21/02/2014, 00:20
... change inducing the release of the product and Mg2+, thereby leaving an Mg2+ ion and a molecule of the substrate bound to the subunit capable of catalyzing hydrolysis Therefore, binding of Mg2+ in ... lower than KS2) Fig The influence of Mg2+ on the kinetic properties of APase from E coli The influence of Mg2+ on the kinetic properties of APase from E coli at a Zn2+ to dimer ratio of : in 2A2M1P ... influence of Mg2+ on the kinetic properties of APase from E coli The influence of Mg2+ on the kinetic properties of APase from E coli in the presence of a Zn2+ to dimer ratio of : in 2A2M1P buffer,...
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Tài liệu Báo cáo khoa học: Unfolding and aggregation during the thermal denaturation of streptokinase pptx
Ngày tải lên :
21/02/2014, 03:20
... preliminary characterization of the thermally induced aggregation of SK using a variety of techniques The results provide us with some of the properties of these high molecular mass aggregates and help ... the fitting and correspond to the values obtained for the isolated domains The uncertainties of the parameters correspond to the standard errors obtained in the fittings Reproducibility of Tm and ... equations using the following set of parameters: a linear heat capacity function for the native state, Cp(N); the enthalpies and the heat capacities of the unfolded state, DHU and DCpU, and of the aggregate,...
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Tài liệu Báo cáo Y học: Evolution of the enzymes of the citric acid cycle and the glyoxylate cycle of higher plants pdf
Ngày tải lên :
22/02/2014, 04:20
... investigate the evolution of the enzymes of the pyruvate dehydrogenase (PDH) complex, the tricarboxylic acid cycle, and the glyoxylate cycle by examining the individual phylogenies of the 21 subunits ... overview of the patterns of similarity for the enzymes of these pathways in plants and the relationships of their differentially compartmentalized isoenzymes Condensing the information from many ... structure, and that some of the subunits of PDH, OGDH and OADH are related The functional and evolutionary relationships between the subunits of these enzymes are somewhat complicated In a nutshell, the...
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