purification separation and identifi cation of the human mtdna polymerase with and without its accessory subunit
Báo cáo khoa học: Substrate specificity of the human UDP-glucuronosyltransferase UGT2B4 and UGT2B7 Identification of a critical aromatic amino acid residue at position 33 doc
Ngày tải lên :
23/03/2014, 09:21
... experiments The film was exposed for four days in the case of UGT2B4 and UGT2B4F33Y and for one week in the case of UGT2B4Y33L (Table 1) In the case of 4-hydroxyestrone and 17-epiestriol, the Km values of ... that the Km and Vmax values of UGT2B4F33Y towards HDCA and 4-hydroxyestrone were increased by 3.5- and twofold, and by 4.6- and three-fold, respectively, compared with UGT2B4 (Table 1) These ... the Vmax values These data suggest that the mutations primarily affect binding of the substrates, but they not rule out the possibility of a reduced access of the substrate to the catalytic site...
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Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc
Ngày tải lên :
24/03/2014, 00:21
... Gly-Ser, in-frame with the codon for Pro2 of the receptor cDNA The codon for the last used amino acid of the receptor (Ser412 in case of the full-length receptor and Ala316 for the truncated receptor) ... concentrations of 10±20 times their Ki value Without ligand, a half-life of 33 days was obtained This increased to 77 days in the presence of NECA (agonist) and 53 days in the presence of theophylline ... solubilization and puri cation; m, 0.2% CHS in solubilization buer and no CHS in buer NiA and NiB; s, 0.2% CHS in solubilization buer and 0.02% in buers NiA and NiB The quanti cation of interactions with...
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Báo cáo khoa học: Expression, purification and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein pot
Ngày tải lên :
31/03/2014, 01:20
... (JASCO) Fig Alignment of the sequences of the second Kunitz-modules of the human and fugu WFIKKN proteins (wfikkn_hu_2; wfikkn_fugu_2) with the second Kunitz-domains of the human and the two fugu WFIKKNRP ... determinant of the hydrophobic S¢2 binding pocket of trypsins is the side-chain of Tyr151 [18] The importance of this residue is underlined by the fact that in the case of the second Kunitz-domain of ... basis of the known sequence of the human WFIKKN mRNA (GenBank accession number AF422194) we have designed PCR primers for the ampli cation of the cDNA segment encoding its second Kunitz-domain The...
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Contrastive analysis on english and vietnames proverbs referring to parts of the human body
Ngày tải lên :
12/12/2013, 00:03
... body The position of proverbs referring to part of the human body Parts of the human body 21 21 21 The classification of parts of the human body according to position Graduation thesis ... understand the equality, sympathy of the husband and wise the function of education is to contribute to directing the peoples feelings towards the good orientation in the relationship of the husband ... diversified (multiple form) The classification of parts of the human body according to the position In the system of the proverbs, the system of words referring to parts of the human body is divided...
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The transference of meaning through class of words denoting parts of the human body in english and vietnames
Ngày tải lên :
18/12/2013, 21:45
... earth The hand of a child - The hand of a lock The heart of a man - The heart of a matter 37 The tongue of a man - The tongue of a shoe The tooth of a person - The tooth of a comb The head of a ... hands of a farm 13 The heel of a person - The heel of the hand 14 One's leg - The leg of a trip 15 One's brain - The brains of a family 16 The bosom of a person - The bosom of the church 17 The ... The head of the page The scar left on one's kin - The scars of war The arm of a person - The arm of jacket 10 One's breast - The chest 11 One's foot - The foot of sock 12 One's hands - The hands...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
Ngày tải lên :
15/02/2014, 01:20
... from the C-terminus (Fig 1A) The two subunits in the asymmetric unit, together with two symmetry-related subunits, form a plausible tetramer similar to the tetramer in HPRT (Fig 1B) However, the ... Asp142, 2¢ and 3¢OH of the ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate of the GMP is hydrogen bonded to main chain and side chain atoms of amino ... accordance with the mechanism of human HPRT [19,25] This conclusion can be made based on the lack of thermal shifts when only the bases were added, whereas the addition of PRPP led to a significant thermal...
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Tài liệu Báo cáo khoa học: Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx
Ngày tải lên :
16/02/2014, 15:20
... performed and the P-values are displayed, as calculated using the Student’s t-test The mean and standard deviations of the absolute numbers of observed colonies are indicated overexpression of OTUB1 and ... experiments, thereby resembling the increased binding of the 37 kDa form of OTUB1 to YpkA (Fig 5A) This is consistent with the notion that phosphorylation of OTUB1 affects the interaction with YpkA, ... agarose without the antibody Immunoprecipitated material was analysed by SDS ⁄ PAGE and silver staining, and the large band corresponding to the expected molecular mass of OTUB1 as well as the area...
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
Ngày tải lên :
18/02/2014, 13:20
... increases the cost of the puri cation, and the require1276 ment for HPLC increases the length and complexity of the puri cation procedure Moreover, as with the studies by Lee et al [21] and Subramanian ... rounds of sonication in buffer, and at the end of each sonication step the suspension was centrifuged and the supernatants (labeled S1, S2 and S3) were stored pending analysis The pellet was then ... understanding of the structure of both the Ab monomer and its various assemblies could help in the design of new therapeutic strategies targeted at preventing the formation or ameliorating the activity...
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Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc
Ngày tải lên :
19/02/2014, 06:20
... in these eukaryotic and archaeal proteins (Fig 1A) Like the case of bacterial RecA, the L1 and L2 loops of the eukaryotic Rad51 proteins face A Roles of the HsRad51 L1 and L2 loops inside of their ... Results Strategy of mutational analysis In order to study the functions of the L1 and L2 loops of HsRad51, we examined the effect of replacing the aromatic residues in the L1 and L2 loops with C B Fig ... helical filaments, where the DNA should be located, and are not found at the ATP-binding site or the subunit subunit interface of the Rad51 filament Therefore, the Rad51 L1 and L2 loops may also...
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Tài liệu Báo cáo khoa học: Topology, tinkering and evolution of the human transcription factor network doc
Ngày tải lên :
19/02/2014, 07:20
... compares the studied network with randomized versions of it with the same size and degree distribution The so-called Z-score quantifies the difference between the studied network and an ensemble of randomized ... order of increasing degree in random webs [61] where ji and ki are the degrees of the nodes located at the ends of the i-th link, with i ¼ L Defined in this way, it is such that )1 £ r £ +1, with ... similarities, with the exception of some TAFII and NC2 and NF-Y factors that have histone fold motifs [52] Group G is a small subset that contains all the SMAD proteins of the HTFN and APC and b-catenin-related...
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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
Ngày tải lên :
19/02/2014, 16:20
... positions of the molecular size markers (kDa) are indicated to the left and right of the (C) and (D), respectively, whilst the position of the Oct-1 (98 kDa approximately) and the two major forms of Oct-2 ... promoter (Prm)3 within the human TXA2 receptor (TP) gene that directs expression of TPb in HEL92.1.7 and HEK293 cells [25] A schematic of the human TP gene highlighting the positions of the previously ... Characterization of the 5¢ untranslated region of alpha and beta isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms Eur J Biochem 269, 4058–4073 26 Quandt...
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Báo cáo khoa học: Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx
Ngày tải lên :
07/03/2014, 00:20
... and that the susceptibility to proteolytic degradation of the isoforms increased with the number of phosphates linked to the polypeptide chains The different position of the groups bound to the ... together with identical aliquots of the untreated proteins (Fig 9A) The degradation process was evaluated on the basis of the ratio between the integrated areas of the intact protein in the treated ... the percentage of the ratio between the spot area of the degraded and that of the untreated protein measured using IMAGE J 1.37V software the presence of proteins eluting after the albumin peak,...
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Báo cáo khoa học: Novel repressor of the human FMR1 gene ) identification ¨ of p56 human (GCC)n-binding protein as a Kruppel-like transcription factor ZF5 ppt
Ngày tải lên :
07/03/2014, 05:20
... Identi cation of a GC element in the promotor of the murine L32 ribosomal protein L32 gene and the 3¢-terminal region of the human apolipoprotein A-I gene, capable of binding with murine and human ... cells interacts with GCC-elements of the rpL32 promoter and the 5¢-UTR of the human FMR1 gene We further identify p56 as the Kruppel¨ like transcription factor ZF5 We show, for the first time, ... affinities to the protein of interest In the case of the rpL32 fragment or the (GCC)9 sequence, there are several repeated ZF5-binding sites within a single oligonucleotide Therefore, the affinity of those...
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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc
Ngày tải lên :
07/03/2014, 12:20
... both MAPKs, ERK1 ⁄ and p38, in the signaling pathways, consistent with the inhibition of the antiproliferative activity of ATP by the MEK1 ⁄ inhibitors PD98059 and UO126 and the p38 MAPK inhibitor ... reversed the inhibition of LXF-289 cell proliferation by ATP Further indirect support for the involvement of ERK1 ⁄ is derived from the observed translocation of ERK1 ⁄ to the nucleus The translocation ... required for the initiation of DNA replication during S phase, or (b) the recruitment of the initiation factor Cdc45, which is required for the elongation phase of replication, or (c) the replication...
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Báo cáo khoa học: Identification of NF1 as a silencer protein of the human adenine nucleotide translocase-2 gene pptx
Ngày tải lên :
07/03/2014, 15:20
... loaded with 0.25 mL of sample The column was eluted at 0.1 mLÆmin)1 Fractions of 0.5 mL were collected after the void volume of the column SDS/PAGE and protein identi cation by MALDI-TOF MS Samples ... www.matrixscience.com) Results Puri cation and identi cation of the silencer binding proteins The promoter of the human ANT2 gene contains three repressor regions (see Fig 1A for a summary) The silencer region ... eliminates repression of the HSVtk promoter in transfected HeLa cells [14] To study the roles of Site-2 and Site-3 individually, they were mutated and placed in front of the HSVtk promoter These constructs...
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Báo cáo khoa học: The stop transfer sequence of the human UDPglucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms docx
Ngày tải lên :
07/03/2014, 16:20
... membrane expression [6] In the absence of positive transport signals, the localization of a protein in the ER may result from the properties of the TMD and its interaction with the membranes It has ... consisting of 16 exons The isoforms are generated by alternative splicing of exon to the four common exons 2–5 resulting in isoforms with an identical C-terminal half of the protein [15] and a unique ... agreement, the staining pattern of the chimeric protein overlapped with that of calnexin, as well as with that of the Golgi marker protein, GM130 (Fig 3B) Cell-surface expression of the protein...
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Báo cáo khoa học: Roles of 1-Cys peroxiredoxin in haem detoxification in the human malaria parasite Plasmodium falciparum potx
Ngày tải lên :
07/03/2014, 17:20
... (Fig 1C) The rate of FP degradation was [FP]-dependent with an apparent Km of 71 lm and a Vmax of 2.4 nmolÆmin)1 (Fig 1D) The rate in the presence of rPf1-Cys-Prx was also [FP]dependent with an ... [13,17,20] The thiol dependency of Pf1-Cys-Prx requires further study [8,9,20], and such information will provide further insights into the physiological functions of this protein On the other hand, ... examined with the recombinant protein We evaluated the effect of rPf1-Cys-Prx on the release of iron from the degradation reaction Thirty micromoles of FP released 0.36 ± 0.03 lgÆmL)1 (6.5 lm) of iron...
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Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx
Ngày tải lên :
07/03/2014, 17:20
... further our knowledge with respect to the mechanisms of action of these proteins, it is crucial that we define the precise boundaries of the STI1-homologous domain and compare the structures of these ... [33] The errors of R1 and R2 were estimated from the errors in the single exponential decay fitting, and those of the 15N-1H NOEs were obtained from the difference of two independent experiments The ... structure of XPCB–hHR23A [13,20] Both overall A B Fig Proteins with STI1-homologous domain and the sequence alignment of the XPCB domain with its homologs (A) Domain presentations of the STI1-homologous...
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Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc
Ngày tải lên :
15/03/2014, 10:20
... specific activity, with Hill coefficients of 2.4 and 1.9 and dissociation constants (KdHill) of and lm for hGBP5a ⁄ b and hGBP5ta, respectively The proteins are very similar in terms of their enzymatic ... an elongated shape of the protein The same behavior is observed in the presence of GDPÆAlFx Because of the unexpected observation of a monomeric protein species with both GDP and GDPÆAlFx, we tested ... or in the presence of GTP As with hGBP5ta, the complex is increased to the size of a tetramer after binding of GppNHp, and binding of GTPcS again shows an intermediate behavior (Fig 5B) The elution...
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Báo cáo khoa học: Involvement of NF-jB subunit p65 and retinoic acid receptors, RARa and RXRa, in transcriptional regulation of the human GnRH II gene pot
Ngày tải lên :
16/03/2014, 10:20
... effects of ligand-activated RARa and RXRa on the promoter activity of the hGnRH II gene in TE671 and JEG-3 cells In vivo effect of RARa and RXRa with their ligands, ATRA and 9-cisRA, on the promoter ... transfected with RARa and ⁄ or RXRa (without RA treatment), simultaneous ligand activation of RARa and RXRa (cotransfection of RARa and RXRa together with treatment of both ATRA and 9-cisRA) ... up-regulation of the gene without over-expression of RAR In contrast, ligand-activated RAR itself was able to up-regulate the gene to a significant level without the aid of RXR This phenomenon of RXR...
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