purification of starch phosphorylases

PURIFICATION OF ORGANIC CHEMICALS pot

Ngày tải lên : 05/03/2014, 21:20

... agent because of the risk of EXPLOSION with acetone vapour Organic impurities have been removed from acetone by adding 4g of AgN03 in 3Oml of water to 1L of acetone, followed by lOml of M NaOH, ... alumina, followed by five batches of 100-15Og of anhydrous CaC12 (Water content of the solvent was then less than 0.2%) It was shaken for l h with log of 68 Purification of Organic Chemicals P2O5, twice, ... in the presence of 2g CrO3 per lOOml and then 66 Purification of Organic Chemicals fractionally distilling [Orton and Bradfield JCS 960 1924,983 19271 Instead of CrO3,2-5% (w/w) of KMn04, with...

17
652
0

Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc

Ngày tải lên : 07/03/2014, 21:20

... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... pulsation of the column because of elevated pressure [9] When revolution speed was 1200 rpm and ﬂow rate of the mobile phase was 2.5 mL/min, more than 50% of the stationary phase (upper layer of petroleum ... increasing the volume ration of ethyl acetate result in the increasing of the K values of curdione (1), curcumol (2) and germacrone (3), and the decreasing of the K values of curzerene (4), 1,8cineole...

7
502
0

Báo cáo khoa học: A new clan of CBM families based on bioinformatics of starch-binding domains from families CBM20 and CBM21 potx

Ngày tải lên : 07/03/2014, 21:20

... Approximately 10% of all amylolytic enzymes possess a distinct domain enabling binding and degradation of raw starch Certain amylolytic enzymes have this capacity without the presence of a specialized ... joining of the two CBMs into a common clan Results and Discussion Location of SBD modules in CBM20 and CBM21 With regard to the location of the SBD in the polypeptide chain, analysis of recent ... dikinase, a starch phosphorylating enzyme from Arabidopsis thaliana, contains a CBM20 module near the N-terminal end of the protein The N-terminal location is also seen in the case of the majority of...

17
476
0

PURIFICATION OF LABORATORY CHEMICALS pot

Ngày tải lên : 14/03/2014, 20:20

... 68 68 GENERAL METHODS FOR THE PURIFICATION OF CLASSES OF COMPOUNDS PROCEDURES CRITERIA OF PURITY 68 68 69 GENERAL PROCEDURES FOR THE PURIFICATION OF SOME CLASSES OF ORGANIC COMPOUNDS ACETALS ACIDS ... esters of some inorganic acids Sol vents 5 5 6 6 METHODS OF PURIFICATION SOLVENT EXTRACTION OF REAGENTS AND SOLVENTS AND DISTRIBUTION DISTILLATION Techniques Distillation of liquid mixtures Types of ... REMOVAL OF TRACES OF METALS FROM REAGENTS METAL IMPURITIES DISTILLATION SCAVENGER RESINS AND OTHER SUPPORTS USE OF ION EXCHANGE RESINS PRECIPITATION Removal of lead contaminants Removal of iron...

8
286
0

purification of carbon nanotubes

Ngày tải lên : 20/03/2014, 13:06

... yield of SWCNTs with nitric acid treatment depend on the concentration of the nitric acid and the time of reﬂux The treatments of Fig – Mass balance of the normalized weight percentage of all ... the weight of the original carbon sample Furthermore, they found that the yield depended crucially on the ﬂow rate of oxidant, the amount of initial sample, the manner of packing of the carbon, ... enhancing the removal of carbon particles as well as controlling the oxidation rate of carbon A purity of $95% SWCNTs with a yield of 20–50 wt% depending on the purity of raw material was reported...

23
604
0

Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf

Ngày tải lên : 22/03/2014, 21:20

... involved in the synthesis of small chains of this fraction Furthermore, SSIV has been found recently to be involved in the control of the number of starch granules and starch granule initiation ... this protein makes this isoform a potential target for the manipulation of the level and quality of plant starch However, little is known about the role of SSIII in starch synthesis and the structure–function ... Merida A (2007) The phenotype of soluble starch synthase IV defective mutants of Arabidopsis thaliana suggests a novel function of elongation enzymes in the control of starch granule formation Plant...

13
457
0

Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis pot

Ngày tải lên : 29/03/2014, 09:20

... activated by the addition of pyridoxal 5¢-phosphate (PLP), the catalytic cofactor of aminotransferase, to the reaction mixture [2] The addition of PLP did not affect the activities of AT1, AT2 or AT3, ... thermophilus The study of aminotransferases in this bacterium is of interest, ﬁrstly because of the need to characterize biochemically aminotransferases The importance of this is emphasized by ... tree of aminotransferases on the basis of the amino acid sequences The numbers at the nodes are bootstrap conﬁdence values expressed as percentages of 1000 bootstrap replicates The order of the...

10
385
0

Báo cáo khoa học: Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis doc

Ngày tải lên : 31/03/2014, 01:20

... absence of another open reading frame % 1000 bp upstream of the start codon and % 2000 bp downstream of the stop codon of the StP gene The entire open reading frame for StP consisted of 2388 ... His-tagged StP and all StP mutants contained 0.8–1.0 mol of PLP per mol of 90-kDa protomer, as expected if incorporation of cofactor during folding of the recombinant proteins had taken place correctly ... to a different interpretation of the role of Arg234 and Arg242 for oxyanion-dependent stability of StP The stabilization brought about by the presence of 10 mM of phosphate was substantially...

11
444
0

Báo cáo Y học: Comparison of starch branching enzyme I and II from potato ppt

Ngày tải lên : 31/03/2014, 23:20

... Larsson, C.-T., Hofvander, P., Khoshnoodi, J., Ek, B., Rask, L & Larsson, H (1996) Three isoforms of starch synthase and two isoforms of branching enzyme are present in potato tuber starch Plant ... clarify this and whether the effect of phosphate is of relevance in vivo Branching of linear dextrins To obtain a more detailed comparison of the mode of action of SBE I and SBE II, the branching ... degradation of retrograded starch by the method of Andersson et al [21] The linear dextrins were dissolved in a small volume of M KOH and diluted with Tris buffer to a ﬁnal concentration of mg:mL21...

6
490
0

armarego - purification of laboratory chemicals 5e

Ngày tải lên : 04/06/2014, 15:52

... mL of 0.2M Na2HPO4 + 61.5 mL of O IM citric acid Or 18 mL of 0.2M NaOAc + 82 mL of 0.2M acetic acid Either 70 mL of 0.2M NaOAc + 30 mL of 0.2M acetic acid Or 51.5 mL of 0.2M Na2HPO4 + 48.5 mL of ... pH values of selected buffers A knowledge of the pK allows the adjustment of the pH without the need of large excesses of acids or base In the case of inorganic compounds a knowledge of the pK ... 63 mL of 0.2M Na2HPO4 + 37 mL of O IM citric acid 82 mL of M Na2HPO4 + 18 mL of O IM citric acid Either 50 mL of O IM Tris buffer + 29 mL of O IN hydrochloric acid, in 100 mL Or 30 mL of 0.05M...

655
199
0

Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot

Ngày tải lên : 18/06/2014, 18:20

... production and collection of HHV-6A Our first concern was the presence of high concentration of serum in the culture medium Routinely, 10% serum was used for propagation of HHV-6A [19] We tested ... whether the majority of cells in culture were infected with HHV-6A and thereby contributing to efficient release of virions Immunofluorescence analysis showed that approximately 80% of the infected ... Infections of JJHAN cells with HHV-6A (U1102) Infections of JJHAN cells with HHV-6A (U1102) A Quantifications of cellular (diamonds) and extracellular (squares) HHV-6A DNA The log10 of the viral...

11
374
0

Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx

Ngày tải lên : 20/06/2014, 01:20

... production and collection of HHV-6A Our first concern was the presence of high concentration of serum in the culture medium Routinely, 10% serum was used for propagation of HHV-6A [19] We tested ... whether the majority of cells in culture were infected with HHV-6A and thereby contributing to efficient release of virions Immunofluorescence analysis showed that approximately 80% of the infected ... Infections of JJHAN cells with HHV-6A (U1102) Infections of JJHAN cells with HHV-6A (U1102) A Quantifications of cellular (diamonds) and extracellular (squares) HHV-6A DNA The log10 of the viral...

11
359
0

Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc

Ngày tải lên : 20/06/2014, 01:20

... uridine, g/L of glucose, 0.372 mL/L of Dawes Trace 1, 2.14 mL/L of Dawes Trace 2, 0.072 mL/L of Dawes Trace 3, 0.606 mL/L of M calcium chloride dihydrate (CaCl2-2H2O), 0.30 mL/L of 0.43 g/ mL ... number of truncated forms of the protein (Figures 1, 2, 3) Factor Xa cleavage of GP1-MBP 28 60 40 30 20 GP1 17 14 GP2 B 98 62 49 B GP1-MBP MBP 38 28 17 GP1 14 Expression and purification of LASV ... sole members of the Board of Directors of BioFactura, Inc., and have received salary and other compensation from the company, such as founder's stock, as it pertains to the execution of this work...

14
411
0

báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx

Ngày tải lên : 21/06/2014, 02:20

... protein One unit is defined as the amount of enzyme that catalyzes the oxidation of μmol of substrate per minute Results Cloning, expression and purification of 2, 5-diketocamphane 1,2-monooxygenase ... cultivations of the wild type strain P putida NCIMB 10007 with subsequent multiple purification and separation steps of the involved enzymes We report here the first recombinant Figure Operon of the ... though separation of the distinct activities was tried by purification, the presence of impurities could not be excluded Therefore, reproducible and reliable methods for separation and purification...

8
548
0

purification of some heavy metals from paper making

Ngày tải lên : 20/07/2014, 12:47

... distribution characteristics of heavy metals at different parts of the reed parts absorption of Cu (mg/L) absorption of Zn (mg/L) absorption of Pb (mg/L) absorption of Cd (mg/L) root 0.0340+0.0080a ... distribution of four kinds of heavy metals Cu Zn Pb Cd at different parts of the reed Distribution feature of heavy metals in different parts of reed is shown in Table Absorptive capacity of the whole ... This study only considers one kind of wetland plants for the purification of heavy metals from papermaking wastewater But in practice a combination of a variety of plants may be needed to purify...

6
231
0

Báo cáo y học: "Inhibition by Natural Dietary Substances of Gastrointestinal Absorption of Starch and Sucrose in Rats and Pigs: 1. Acute Studies" pot

Ngày tải lên : 08/08/2014, 16:23

... influence the appearance of circulating glucose after sucrose challenge L-Arabinose slowed the absorption of sucrose, but not that of rice starch The inability of any of these agents to influence ... at the initiation of study, were deprived of food and water for hours at the time of study Then, they were given challenges of 200 g sucrose (table sugar) and/or 100 g rice starch individually ... doubling of the initial dose caused further lowering of the absorption of rice starch compared to the standard dose after one and two hours as estimated by the Int J Med Sci 2007, appearance of circulating...

7
394
0

Báo cáo y học: "Inhibition by Natural Dietary Substances of Gastrointestinal Absorption of Starch and Sucrose in Rats 2. Subchronic Studies" pptx

Ngày tải lên : 08/08/2014, 16:23

... acutely with two grams of rice starch One-half hour prior to the CHO challenges and at the time of challenges a total of four ml of water was given to control, or two gram of formulation in four ... addition of Lactobacillus acidophilus and Bifidobacterium bifidum Carbohydrate Tolerance Tests: After baseline blood was drawn, rats received either a gavage of two ml of water alone or two g rice starch, ... two grams of the novel formulation dissolved in a similar volume of water is depicted Mean + SEM shown for eight rats in each group All rats drank a solution of 10% w/v sucrose in place of water...

7
290
0

Báo cáo lâm nghiệp: "Effects of phosphate deficiency on photosynthesis and accumulation of starch and soluble sugars in 1-year-old seedlings of maritime pine (Pinus pinaster Ait)" doc

Ngày tải lên : 08/08/2014, 18:21

... convexity of the quantum response of the potential electron transport of needles and was fixed at 0.79 (Leverenz and Jarvis, 1979) a is the initial slope of the quantum response curve of potential ... 22 °C, 75% of relative humidity, and various levels of CO and under a range of light intensities Spe, 2 cifically, light and CO curves were generated The on total leaf area suming a of the needles ... the effects of P deficiency on the photosynthesis and nonstructural carbohydrate content in maritime pine seedlings The main parameters of the biochemical model of CO assimilation of Farquhar...

10
305
0

báo cáo khoa học: "Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation" pps

Ngày tải lên : 11/08/2014, 00:23

... Comparison of purification yield and protein recovery of three Nickel-magnetic matrices for His-ProT and His-Mre11 recombinant proteins Purification yield was defined as the sum of the percents of the ... concentration of beads, the higher fraction of the protein remained attached to the matrix (Figure 5) Effect of bead concentration In order to clarify the effect of bead concentration on the purification ... considerable amount of non-specific proteins eluted in conjunction with this protein Final purity of the purified proteins is without any doubt an excellent measure of the performance of protein purification...

11
289
0

Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system" docx

Ngày tải lên : 11/08/2014, 21:21

... fragment was about 1840 bp 3.2 Expression and purification of polyhistidine-tagged VP2 of PPV We have previously shown that formation of chimeric VLPs of PPV fusion constructs is feasible in pET-PPV ... products purified by IMAC as described below Page of 2.6 Hemagglutination assay Following the method of Senda et al [20], two-fold dilutions of samples of VP2 protein were prepared, mixed with guinea ... rpm) to a concentration of about × 106 cells per ml of culture medium (30 ml growing volume) and infected with the recombinant viruses at a multiplicity of infection (MOI) of 2-3 At 72 h postinfection...

6
387
0