... activity of ticks 50 3.1.1 Protein quantification of crude salivaryglandextracts 50 3.1.2 Activity of crude salivaryglandextracts 50 3.1.3 Purificationofsalivaryglandextracts51 ... 2.1 Salivary glands and extractsSalivarygland and salivaryglandextractsof ticks were obtained from collaborators RNDr Mirko Slovak and RNDr Maria Kazimorova from the Slovak Academy of Sciences, ... Anticoagulant activity of crude R pulchellus salivaryglandextracts51 Figure 3.2 Anticoagulant profile of R pulchellus SGEs 52 Figure 3.3 Anticoagulant profile of D reticulatus SGEs...
... analysis of a T infestans salivarygland extract and puried recombinant triafestin-1 and triafestin-2 A crude salivarygland extract (lane 1), lg of recombinant triafestin-1 (lane 2) and lg of recombinant ... components of the saliva emphasizes the biological importance of inhibition of the kallikreinkinin system for bloodfeeding behavior These observations may be an example of the convergent evolution ofsalivary ... Results cDNA cloning and expression of recombinant triafestin-1 and triafestin-2 A cDNA library was constructed from the salivary glands of unfed fth instar nymphs of T infestans cDNA fragments from...
... of T infestans saliva Fig Western blot analysis of triplatin-1 and -2 from the salivaryglandof T infestans Extracts from a pair ofsalivary glands, recombinant triplatin-1 and recombinant triplatin-2 ... Isolation and sequencing of cDNA clones Salivary glands of T infestans were dissected from thoraces of unfed adults, and poly A(+) RNA was isolated from 30 pairs ofsalivary glands using a MicroPrep ... Karczewski J & Connolly TM (1992) An inhibitor of collagen-stimulated platelet activation from the salivary glands of the Haementetia of cinalis leech II Cloning of the cDNA and expression J Biol Chem...
... homozygous double congenic strain The purpose of the present study was to monitor the influence of each of these two loci on the incidence and severity of sialadenitis, and to identify genes located ... samples included high amounts (from to μg/μl) of good quality RNA Salivarygland gene expression The total RNA was submitted to the RNA-in/data-out service of the Norwegian Microarray Consortium Core ... significance level of 0.05 For each assay, one reaction without Multiscribe RT enzyme was included, to ensure RNA specificity of the assays Results Influence of NOD Idd5 and Nss1 loci on severity of sialadenitis...
... Diseases of the salivary glands Inflammatory salivarygland diseases, next to benign neoplasms, are the most frequent causes ofsalivarygland swelling in juvenile age [3] The acute forms of sialadenitis ... to Eneroth [13] salivarygland tumors make up 0.3% of all human tumors, and less than 10% of all juvenile head and neck tumors are located in the salivary glands [14] Only 1% of all head and ... instance of persisting postoperative nerve injury or symptomatic Frey’s syndrome following total extirpation of the parotid gland due to chronic sialadenitis Page of Tumors of the salivary glands...
... differentiation of secretory acinus and related ducts, the functional unit ofsalivary glands Immunohistochemical studies demonstrated different degrees of EGFR expression in several salivarygland carcinomas, ... palliative management of advanced salivarygland cancers J Clin Oncol 2006, 24:2673-2678 Agulnik M, Siu LL: An update on the systemic therapy of malignant salivarygland cancers: role of chemotherapy ... antitumor activity of anti-EGFR targeted agents in the salivarygland neoplasms In a phase II study of cetuximab monotherapy in 22 patients with recurrent/metastatic salivarygland carcinomas,...
... the presence of proteins able to bind to the four DENV serotypes in mosquito salivaryglandextracts Because SGE might contain both salivarygland tissue (basal lamina or salivarygland epithelial ... phenylmethylsulfonyl fluoride and 10 μg/ ml of aprotinine) Each vial contained about 1,500 pairs ofsalivary glands and was stored at -80°C until needed [8] Salivary glands were then thawed and disrupted ... monoclonal polynesiensis salivary glands DENV-binding proteins from Ae polynesiensis salivary glands detected with anti-E monoclonal antibodies Ae polynesiensis salivaryglandextracts were treated as...
... disruption of mitochondrial membrane potential, activation of multiple caspases, arrest of cell cycle progression, and induction of differentiation The positive effect of this compound on other types of ... Department of Nutrition and Dietetics, King’s College London and honorary professor of clinical biochemistry in the Department of Clinical Biochemistry, King’s College Hospital He is also director of ... Medicine of the School of Medicine He is currently professor of health promotion sciences in the Mel and Enid Zuckerman Arizona College of Public Health Dr Watson is a member of several national...
... M Martins et al using twofold dilutions ofsalivary glands extracts, or stock solutions of the peptide proP7 Recombinant protein expression and purification An NL region of preprotrialysin (spanning ... amounts ofsalivarygland homogenates prepared in the presence (d) or absence (s) of APMSF In (A) the number of surviving cells was determined in a hemocytometer, and the percentage of lysis ... found in saliva is the active form of trialysin, and suggesting that the presence of 10–15 amino acids of the proregion of protrialysin found in the glands (instead of the predicted 33-mer acidic...
... the salivary glands of patients with SS There is clear evidence of an inflammatory environment with presentation of self-antigens characteristic of SS [22] that may permit the production of autoantibodies ... the CDR of VH and VL gene rearrangements of B cells obtained by minor salivarygland biopsies from two patients with SS [6] Detailed analyses of the frequency of occurrence and mutations of the ... (5.4 [98/18] of peripheral blood versus 3.7 [188 /51] of the parotid gland) were compared between the two compartments seemed to be selected positively in the gland A large number of silent mutations...
... profile but not with histopathologic changes in the salivarygland Arthritis Rheum 2006, 54:23002305 Delaleu N, Immervoll H, Cornelius J, Jonsson R: Biomarker profiles in serum and saliva of ... and the development of subsequent stages of glomerulonephritis in these rats [9] In MRLlpr mice a truncated fractalkine analogue with the capability of antagonizing the actions of fractalkine also ... initiation, accumulation and retention of inflammatory cells in the salivary glands This corresponds to properties of fractalkine Page of (page number not for citation purposes) reported in other inflammatory...
... negative in primary adenocarcinomas of the lung, a salivarygland origin was most likely Immunohistochemistry might also aid in the differential diagnosis ofsalivarygland carcinoma types In the present ... incidence of the adenocarcinoma (NOS) is decreasing with the establishment of new neoplastic entities of the salivary glands, this carcinoma still occurs and should be taken into account in case of ... Adenocarcinoma ofsalivary origin: Clinicopathologic study of 204 patients Am J Surg 1982, 144:423-31 Matsuba HM, Mauney M, Simpson JR, Thawley SE, Pikul FJ: Adenocarcinomas of major and minor salivary gland...
... Myoepithelioma is a rare benign tumor of the salivary glands and is usually seen in the parotid gland and the minor salivary glands It was once considered to be a type of pleomorphic adenoma (PA), but ... tumor is usually located in the parotid gland and the minor salivary glands of the soft palate and represents less than 1% of all salivarygland tumors [2] Several authors now consider this tumor ... epithelial neoplasms In Tumors ofSalivary Glands AFIP; 2002: 57-68 Ferri E, Pavon I, Armato E, Cavaleri S, Capuzzo P, Ianniello F: Myoepithelioma of a minor salivaryglandof the cheek: case report...
... with tumor-like lesions of the salivary glands at the outpatient care of an ENT-department (university hospital) 30.7% of the patients with tumor-like lesions in salivary glands underwent an imaging ... sensitivity of 98%, a specificity of 52% and an accuracy of 84% According to Takashima et al., MRI achieved a sensitivity of 60%, a specificity of 88% and an accuracy of 81% in the assessment of tumor ... Adenoma of the left parotid gland Pleomorphic Adenoma of the left parotid gland: Ultrasound image axial and Ultrasound image transversal ultrasound head or located deeper in the salivary gland, ...
... 1C) The effect of the length of the incubation period on the rate of inhibition of the HIV-1 infection of the CEM Table Mucin toxicity Sample Con CEM SS cells % of dead cells % of live cells MUC5B ... of the salivary glands which results in a decline in the amount of saliva and a change in its constituents [10] This in turn may affect the glycosylation pattern or sugar composition of the salivary ... between salivary MUC5B and MUC7 mucins Habte et al Virology Journal 2010, 7:269 http://www.virologyj.com/content/7/1/269 Page of Figure Anti-HIV-1 activities ofsalivary MUC5B and MUC7 of different...
... but a significant lowering 173 of average SBP by day 30 and throughout the remainder of the study The entire BP profile and heart rate were assessed near the end of the study as shown in Table ... Systolic blood pressure (SBP) of SD rats under Control conditions over a period of 458 days (n=8) The present study began on Day 338 of their lifespan The start of the present study is indicated ... has fallen from 56% to 46% of energy intake over the first 75 years of the 21st century, this has been wholly at the expense of complex CHO, because the consumption of refined CHO, especially...
... the chemopreventive effect of the gallic acid derivatives Because grape is a rich source of procyanidins Antiproliferative properties of natural extracts Fig Structure of the major polyphenols ... compounds of interest in the prevention of colon cancer In the present study, we investigated the relationship of different structural factors of procyanidins, such as the mean degree of polymerization ... properties of natural extracts D Lizarraga et al Table Comparative chemical characteristics and HT29 cell growth inhibition of grape and pine fractions Percentage of galloylation (%G), mean degree of...
... periphery of the protein (Fig 5A) Unexpectedly, the volume of the main cavity is one of the smallest among lipocalins of known three-dimensional structures The ˚ cavity of MUP has a volume of 510 ... respectively, in isoform A of SAL, the structure of which is described here, and are replaced in isoform B by Ala and Val, respectively Replacement of residues 43 and 75 with those of isoform B in the ... density maps SAL is formed of the classical Ó FEBS 2002 Boar salivary lipocalin structure (Eur J Biochem 269) 2 451 Fig Amino-acid sequence of SAL aligned with those of other mammalian lipocalins...
... anticoagulants of H longicornis It was previously reported that anticoagulants are present in the salivary glands of H longicornis [24] According to this report, an extract of the salivary glands prolonged ... Chemical Industry, Osaka, Japan Mass sequence analysis of cDNA clones H longicornis salivarygland mRNA was isolated from 10 pairs of glands of ticks at three distinct feeding stages (unfed, slow ... The prolongation of fibrin clot formation was measured using the coagulometer Assay of effects of madanin and on activation of factor V and factor VIII by thrombin The effect of the madanins on...
... the conversion of the Au(III), X the value of any of the five parameters of each broth, Cov(X, Y) the covariance value and rxy the correlation coefficient of X and Y with a range of [-1, 1], E the ... Effects of the Parameters on the Conversion of Au(III) Original pH During biosynthesis of GNPs, all of the ultimate reaction solutions possessed the characteristic red color, indicating generation of ... Original pH of the broths versus conversion of Au(III) the content of flavonoids, reducing sugars and proteins, as well as the anti-oxidant capability of the foliar broths should reflect the role of each...