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purification of salivary gland extracts 51

Characterization of novel anticoagulants from hematophagous arthropods

Characterization of novel anticoagulants from hematophagous arthropods

Thạc sĩ - Cao học

... activity of ticks 50 3.1.1 Protein quantification of crude salivary gland extracts 50 3.1.2 Activity of crude salivary gland extracts 50 3.1.3 Purification of salivary gland extracts 51 ... 2.1 Salivary glands and extracts Salivary gland and salivary gland extracts of ticks were obtained from collaborators RNDr Mirko Slovak and RNDr Maria Kazimorova from the Slovak Academy of Sciences, ... Anticoagulant activity of crude R pulchellus salivary gland extracts 51 Figure 3.2 Anticoagulant profile of R pulchellus SGEs 52 Figure 3.3 Anticoagulant profile of D reticulatus SGEs...
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Báo cáo khoa học: Identification and characterization of plasma kallikrein–kinin system inhibitors from salivary glands of the blood-sucking insect Triatoma infestans pptx

Báo cáo khoa học: Identification and characterization of plasma kallikrein–kinin system inhibitors from salivary glands of the blood-sucking insect Triatoma infestans pptx

Báo cáo khoa học

... analysis of a T infestans salivary gland extract and puried recombinant triafestin-1 and triafestin-2 A crude salivary gland extract (lane 1), lg of recombinant triafestin-1 (lane 2) and lg of recombinant ... components of the saliva emphasizes the biological importance of inhibition of the kallikreinkinin system for bloodfeeding behavior These observations may be an example of the convergent evolution of salivary ... Results cDNA cloning and expression of recombinant triafestin-1 and triafestin-2 A cDNA library was constructed from the salivary glands of unfed fth instar nymphs of T infestans cDNA fragments from...
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Báo cáo khoa học: Identification and characterization of a collagen-induced platelet aggregation inhibitor, triplatin, from salivary glands of the assassin bug, Triatoma infestans ppt

Báo cáo khoa học: Identification and characterization of a collagen-induced platelet aggregation inhibitor, triplatin, from salivary glands of the assassin bug, Triatoma infestans ppt

Báo cáo khoa học

... of T infestans saliva Fig Western blot analysis of triplatin-1 and -2 from the salivary gland of T infestans Extracts from a pair of salivary glands, recombinant triplatin-1 and recombinant triplatin-2 ... Isolation and sequencing of cDNA clones Salivary glands of T infestans were dissected from thoraces of unfed adults, and poly A(+) RNA was isolated from 30 pairs of salivary glands using a MicroPrep ... Karczewski J & Connolly TM (1992) An inhibitor of collagen-stimulated platelet activation from the salivary glands of the Haementetia of cinalis leech II Cloning of the cDNA and expression J Biol Chem...
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Báo cáo y học:

Báo cáo y học: "The influence of the NOD Nss1/Idd5 loci on sialadenitis and gene expression in salivary glands of congenic mice" docx

Báo cáo khoa học

... homozygous double congenic strain The purpose of the present study was to monitor the influence of each of these two loci on the incidence and severity of sialadenitis, and to identify genes located ... samples included high amounts (from to μg/μl) of good quality RNA Salivary gland gene expression The total RNA was submitted to the RNA-in/data-out service of the Norwegian Microarray Consortium Core ... significance level of 0.05 For each assay, one reaction without Multiscribe RT enzyme was included, to ensure RNA specificity of the assays Results Influence of NOD Idd5 and Nss1 loci on severity of sialadenitis...
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báo cáo khoa học:

báo cáo khoa học: " Diseases of the salivary glands in infants and adolescents" ppt

Báo cáo khoa học

... Diseases of the salivary glands Inflammatory salivary gland diseases, next to benign neoplasms, are the most frequent causes of salivary gland swelling in juvenile age [3] The acute forms of sialadenitis ... to Eneroth [13] salivary gland tumors make up 0.3% of all human tumors, and less than 10% of all juvenile head and neck tumors are located in the salivary glands [14] Only 1% of all head and ... instance of persisting postoperative nerve injury or symptomatic Frey’s syndrome following total extirpation of the parotid gland due to chronic sialadenitis Page of Tumors of the salivary glands...
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Báo cáo y học:

Báo cáo y học: "Cetuximab in the treatment of metastatic mucoepidermoid carcinoma of the salivary glands: A case report and review of literature" doc

Báo cáo khoa học

... differentiation of secretory acinus and related ducts, the functional unit of salivary glands Immunohistochemical studies demonstrated different degrees of EGFR expression in several salivary gland carcinomas, ... palliative management of advanced salivary gland cancers J Clin Oncol 2006, 24:2673-2678 Agulnik M, Siu LL: An update on the systemic therapy of malignant salivary gland cancers: role of chemotherapy ... antitumor activity of anti-EGFR targeted agents in the salivary gland neoplasms In a phase II study of cetuximab monotherapy in 22 patients with recurrent/metastatic salivary gland carcinomas,...
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Báo cáo khoa học:

Báo cáo khoa học: "Dengue viruses binding proteins from Aedes aegypti and Aedes polynesiensis salivary glands" ppt

Báo cáo khoa học

... the presence of proteins able to bind to the four DENV serotypes in mosquito salivary gland extracts Because SGE might contain both salivary gland tissue (basal lamina or salivary gland epithelial ... phenylmethylsulfonyl fluoride and 10 μg/ ml of aprotinine) Each vial contained about 1,500 pairs of salivary glands and was stored at -80°C until needed [8] Salivary glands were then thawed and disrupted ... monoclonal polynesiensis salivary glands DENV-binding proteins from Ae polynesiensis salivary glands detected with anti-E monoclonal antibodies Ae polynesiensis salivary gland extracts were treated as...
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Bioactive Foods and Extracts ppt

Bioactive Foods and Extracts ppt

Sức khỏe giới tính

... disruption of mitochondrial membrane potential, activation of multiple caspases, arrest of cell cycle progression, and induction of differentiation The positive effect of this compound on other types of ... Department of Nutrition and Dietetics, King’s College London and honorary professor of clinical biochemistry in the Department of Clinical Biochemistry, King’s College Hospital He is also director of ... Medicine of the School of Medicine He is currently professor of health promotion sciences in the Mel and Enid Zuckerman Arizona College of Public Health Dr Watson is a member of several national...
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Báo cáo khoa học: A short proregion of trialysin, a pore-forming protein of Triatoma infestans salivary glands, controls activity by folding the N-terminal lytic motif pdf

Báo cáo khoa học: A short proregion of trialysin, a pore-forming protein of Triatoma infestans salivary glands, controls activity by folding the N-terminal lytic motif pdf

Báo cáo khoa học

... M Martins et al using twofold dilutions of salivary glands extracts, or stock solutions of the peptide proP7 Recombinant protein expression and purification An NL region of preprotrialysin (spanning ... amounts of salivary gland homogenates prepared in the presence (d) or absence (s) of APMSF In (A) the number of surviving cells was determined in a hemocytometer, and the percentage of lysis ... found in saliva is the active form of trialysin, and suggesting that the presence of 10–15 amino acids of the proregion of protrialysin found in the glands (instead of the predicted 33-mer acidic...
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Báo cáo y học:

Báo cáo y học: "Analysis of immunoglobulin light chain rearrangements in the salivary gland and blood of a patient with Sjögren’s syndrome" pptx

Báo cáo khoa học

... the salivary glands of patients with SS There is clear evidence of an inflammatory environment with presentation of self-antigens characteristic of SS [22] that may permit the production of autoantibodies ... the CDR of VH and VL gene rearrangements of B cells obtained by minor salivary gland biopsies from two patients with SS [6] Detailed analyses of the frequency of occurrence and mutations of the ... (5.4 [98/18] of peripheral blood versus 3.7 [188 /51] of the parotid gland) were compared between the two compartments seemed to be selected positively in the gland A large number of silent mutations...
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Báo cáo y học:

Báo cáo y học: "Altered fractalkine cleavage results in an organ-specific 17 kDa fractalkine fragment in salivary glands of NOD mice" pot

Báo cáo khoa học

... profile but not with histopathologic changes in the salivary gland Arthritis Rheum 2006, 54:23002305 Delaleu N, Immervoll H, Cornelius J, Jonsson R: Biomarker profiles in serum and saliva of ... and the development of subsequent stages of glomerulonephritis in these rats [9] In MRLlpr mice a truncated fractalkine analogue with the capability of antagonizing the actions of fractalkine also ... initiation, accumulation and retention of inflammatory cells in the salivary glands This corresponds to properties of fractalkine Page of (page number not for citation purposes) reported in other inflammatory...
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báo cáo khoa học:

báo cáo khoa học: " The buccal minor salivary glands as starting point for a metastasizing adenocarcinoma – report of a case" pptx

Báo cáo khoa học

... negative in primary adenocarcinomas of the lung, a salivary gland origin was most likely Immunohistochemistry might also aid in the differential diagnosis of salivary gland carcinoma types In the present ... incidence of the adenocarcinoma (NOS) is decreasing with the establishment of new neoplastic entities of the salivary glands, this carcinoma still occurs and should be taken into account in case of ... Adenocarcinoma of salivary origin: Clinicopathologic study of 204 patients Am J Surg 1982, 144:423-31 Matsuba HM, Mauney M, Simpson JR, Thawley SE, Pikul FJ: Adenocarcinomas of major and minor salivary gland...
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báo cáo khoa học:

báo cáo khoa học: " Plasmacytoid myoepithelioma of minor salivary glands: report of case with emphasis in the " pot

Báo cáo khoa học

... Myoepithelioma is a rare benign tumor of the salivary glands and is usually seen in the parotid gland and the minor salivary glands It was once considered to be a type of pleomorphic adenoma (PA), but ... tumor is usually located in the parotid gland and the minor salivary glands of the soft palate and represents less than 1% of all salivary gland tumors [2] Several authors now consider this tumor ... epithelial neoplasms In Tumors of Salivary Glands AFIP; 2002: 57-68 Ferri E, Pavon I, Armato E, Cavaleri S, Capuzzo P, Ianniello F: Myoepithelioma of a minor salivary gland of the cheek: case report...
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báo cáo khoa học:

báo cáo khoa học:" Neither MRI, CT nor US is superior to diagnose tumors in the salivary glands – an extended case study" ppt

Báo cáo khoa học

... with tumor-like lesions of the salivary glands at the outpatient care of an ENT-department (university hospital) 30.7% of the patients with tumor-like lesions in salivary glands underwent an imaging ... sensitivity of 98%, a specificity of 52% and an accuracy of 84% According to Takashima et al., MRI achieved a sensitivity of 60%, a specificity of 88% and an accuracy of 81% in the assessment of tumor ... Adenoma of the left parotid gland Pleomorphic Adenoma of the left parotid gland: Ultrasound image axial and Ultrasound image transversal ultrasound head or located deeper in the salivary gland, ...
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Báo cáo y học:

Báo cáo y học: " Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts" doc

Báo cáo khoa học

... 1C) The effect of the length of the incubation period on the rate of inhibition of the HIV-1 infection of the CEM Table Mucin toxicity Sample Con CEM SS cells % of dead cells % of live cells MUC5B ... of the salivary glands which results in a decline in the amount of saliva and a change in its constituents [10] This in turn may affect the glycosylation pattern or sugar composition of the salivary ... between salivary MUC5B and MUC7 mucins Habte et al Virology Journal 2010, 7:269 http://www.virologyj.com/content/7/1/269 Page of Figure Anti-HIV-1 activities of salivary MUC5B and MUC7 of different...
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Báo cáo y học:

Báo cáo y học: "Maitake Mushroom Extracts Ameliorate Progressive Hypertension and Other Chronic Metabolic Perturbations in Aging Female Rats"

Y học thưởng thức

... but a significant lowering 173 of average SBP by day 30 and throughout the remainder of the study The entire BP profile and heart rate were assessed near the end of the study as shown in Table ... Systolic blood pressure (SBP) of SD rats under Control conditions over a period of 458 days (n=8) The present study began on Day 338 of their lifespan The start of the present study is indicated ... has fallen from 56% to 46% of energy intake over the first 75 years of the 21st century, this has been wholly at the expense of complex CHO, because the consumption of refined CHO, especially...
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Báo cáo khoa học: The importance of polymerization and galloylation for the antiproliferative properties of procyanidin-rich natural extracts doc

Báo cáo khoa học: The importance of polymerization and galloylation for the antiproliferative properties of procyanidin-rich natural extracts doc

Báo cáo khoa học

... the chemopreventive effect of the gallic acid derivatives Because grape is a rich source of procyanidins Antiproliferative properties of natural extracts Fig Structure of the major polyphenols ... compounds of interest in the prevention of colon cancer In the present study, we investigated the relationship of different structural factors of procyanidins, such as the mean degree of polymerization ... properties of natural extracts D Lizarraga et al Table Comparative chemical characteristics and HT29 cell growth inhibition of grape and pine fractions Percentage of galloylation (%G), mean degree of...
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Báo cáo Y học: Boar salivary lipocalin Three-dimensional X-ray structure and androstenol/androstenone docking simulations pot

Báo cáo Y học: Boar salivary lipocalin Three-dimensional X-ray structure and androstenol/androstenone docking simulations pot

Báo cáo khoa học

... periphery of the protein (Fig 5A) Unexpectedly, the volume of the main cavity is one of the smallest among lipocalins of known three-dimensional structures The ˚ cavity of MUP has a volume of  510 ... respectively, in isoform A of SAL, the structure of which is described here, and are replaced in isoform B by Ala and Val, respectively Replacement of residues 43 and 75 with those of isoform B in the ... density maps SAL is formed of the classical Ó FEBS 2002 Boar salivary lipocalin structure (Eur J Biochem 269) 2 451 Fig Amino-acid sequence of SAL aligned with those of other mammalian lipocalins...
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Báo cáo khoa học: Identification and characterization of novel salivary thrombin inhibitors from the ixodidae tick, Haemaphysalis longicornis doc

Báo cáo khoa học: Identification and characterization of novel salivary thrombin inhibitors from the ixodidae tick, Haemaphysalis longicornis doc

Báo cáo khoa học

... anticoagulants of H longicornis It was previously reported that anticoagulants are present in the salivary glands of H longicornis [24] According to this report, an extract of the salivary glands prolonged ... Chemical Industry, Osaka, Japan Mass sequence analysis of cDNA clones H longicornis salivary gland mRNA was isolated from 10 pairs of glands of ticks at three distinct feeding stages (unfed, slow ... The prolongation of fibrin clot formation was measured using the coagulometer Assay of effects of madanin and on activation of factor V and factor VIII by thrombin The effect of the madanins on...
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Báo cáo hóa học:

Báo cáo hóa học: " Biosynthesis of Gold Nanoparticles by Foliar Broths: Roles of Biocompounds and Other Attributes of the Extracts" pptx

Hóa học - Dầu khí

... the conversion of the Au(III), X the value of any of the five parameters of each broth, Cov(X, Y) the covariance value and rxy the correlation coefficient of X and Y with a range of [-1, 1], E the ... Effects of the Parameters on the Conversion of Au(III) Original pH During biosynthesis of GNPs, all of the ultimate reaction solutions possessed the characteristic red color, indicating generation of ... Original pH of the broths versus conversion of Au(III) the content of flavonoids, reducing sugars and proteins, as well as the anti-oxidant capability of the foliar broths should reflect the role of each...
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