purification of polyphenol oxidases
PURIFICATION OF ORGANIC CHEMICALS pot
... agent because of the risk of EXPLOSION with acetone vapour Organic impurities have been removed from acetone by adding 4g of AgN03 in 3Oml of water to 1L of acetone, followed by lOml of M NaOH, ... alumina, followed by five batches of 100-15Og of anhydrous CaC12 (Water content of the solvent was then less than 0.2%) It was shaken for l h with log of 68 Purification of Organic Chemicals P2O5, twice, ... in the presence of 2g CrO3 per lOOml and then 66 Purification of Organic Chemicals fractionally distilling [Orton and Bradfield JCS 960 1924,983 19271 Instead of CrO3,2-5% (w/w) of KMn04, with...
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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc
... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... pulsation of the column because of elevated pressure [9] When revolution speed was 1200 rpm and flow rate of the mobile phase was 2.5 mL/min, more than 50% of the stationary phase (upper layer of petroleum ... increasing the volume ration of ethyl acetate result in the increasing of the K values of curdione (1), curcumol (2) and germacrone (3), and the decreasing of the K values of curzerene (4), 1,8cineole...
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PURIFICATION OF LABORATORY CHEMICALS pot
... 68 68 GENERAL METHODS FOR THE PURIFICATION OF CLASSES OF COMPOUNDS PROCEDURES CRITERIA OF PURITY 68 68 69 GENERAL PROCEDURES FOR THE PURIFICATION OF SOME CLASSES OF ORGANIC COMPOUNDS ACETALS ACIDS ... esters of some inorganic acids Sol vents 5 5 6 6 METHODS OF PURIFICATION SOLVENT EXTRACTION OF REAGENTS AND SOLVENTS AND DISTRIBUTION DISTILLATION Techniques Distillation of liquid mixtures Types of ... REMOVAL OF TRACES OF METALS FROM REAGENTS METAL IMPURITIES DISTILLATION SCAVENGER RESINS AND OTHER SUPPORTS USE OF ION EXCHANGE RESINS PRECIPITATION Removal of lead contaminants Removal of iron...
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purification of carbon nanotubes
... yield of SWCNTs with nitric acid treatment depend on the concentration of the nitric acid and the time of reflux The treatments of Fig – Mass balance of the normalized weight percentage of all ... the weight of the original carbon sample Furthermore, they found that the yield depended crucially on the flow rate of oxidant, the amount of initial sample, the manner of packing of the carbon, ... enhancing the removal of carbon particles as well as controlling the oxidation rate of carbon A purity of $95% SWCNTs with a yield of 20–50 wt% depending on the purity of raw material was reported...
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Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis pot
... activated by the addition of pyridoxal 5¢-phosphate (PLP), the catalytic cofactor of aminotransferase, to the reaction mixture [2] The addition of PLP did not affect the activities of AT1, AT2 or AT3, ... thermophilus The study of aminotransferases in this bacterium is of interest, firstly because of the need to characterize biochemically aminotransferases The importance of this is emphasized by ... tree of aminotransferases on the basis of the amino acid sequences The numbers at the nodes are bootstrap confidence values expressed as percentages of 1000 bootstrap replicates The order of the...
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Antisense expression of polyphenol oxidase genes inhibits enzymatic browning in potato tubers
... Construction of T-DNA vectors carrying antisense PPO cDNAs In order to maximize the chances ofachieving ahigh level of antisense inhibition of PPO gene expression, we designed a series of antisense ... patatin promoter activity" Ofthe large population of transgenic PPO lines generated, a small proportion of lines were not amenable to microtuber induction (
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Polyphenol oxidases in plants and fungi
... contributions to the store of information they not advance the basic understanding of the function of the enzyme and proof of causal relationships between observed phenomena and levels of PPO are mostly ... description of PPO in plant tissues has been the research on the multiplicity of genes coding for PPO, their description and the characterization of the expression pattern of some of these genes Some of ... multiplicity of genes, their differential expression in different parts of the plant and at different stages of development is one of the most important features of recent work on plant PPO The sequences of...
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armarego - purification of laboratory chemicals 5e
... mL of 0.2M Na2HPO4 + 61.5 mL of O IM citric acid Or 18 mL of 0.2M NaOAc + 82 mL of 0.2M acetic acid Either 70 mL of 0.2M NaOAc + 30 mL of 0.2M acetic acid Or 51.5 mL of 0.2M Na2HPO4 + 48.5 mL of ... pH values of selected buffers A knowledge of the pK allows the adjustment of the pH without the need of large excesses of acids or base In the case of inorganic compounds a knowledge of the pK ... 63 mL of 0.2M Na2HPO4 + 37 mL of O IM citric acid 82 mL of M Na2HPO4 + 18 mL of O IM citric acid Either 50 mL of O IM Tris buffer + 29 mL of O IN hydrochloric acid, in 100 mL Or 30 mL of 0.05M...
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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot
... production and collection of HHV-6A Our first concern was the presence of high concentration of serum in the culture medium Routinely, 10% serum was used for propagation of HHV-6A [19] We tested ... whether the majority of cells in culture were infected with HHV-6A and thereby contributing to efficient release of virions Immunofluorescence analysis showed that approximately 80% of the infected ... Infections of JJHAN cells with HHV-6A (U1102) Infections of JJHAN cells with HHV-6A (U1102) A Quantifications of cellular (diamonds) and extracellular (squares) HHV-6A DNA The log10 of the viral...
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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx
... production and collection of HHV-6A Our first concern was the presence of high concentration of serum in the culture medium Routinely, 10% serum was used for propagation of HHV-6A [19] We tested ... whether the majority of cells in culture were infected with HHV-6A and thereby contributing to efficient release of virions Immunofluorescence analysis showed that approximately 80% of the infected ... Infections of JJHAN cells with HHV-6A (U1102) Infections of JJHAN cells with HHV-6A (U1102) A Quantifications of cellular (diamonds) and extracellular (squares) HHV-6A DNA The log10 of the viral...
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Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc
... uridine, g/L of glucose, 0.372 mL/L of Dawes Trace 1, 2.14 mL/L of Dawes Trace 2, 0.072 mL/L of Dawes Trace 3, 0.606 mL/L of M calcium chloride dihydrate (CaCl2-2H2O), 0.30 mL/L of 0.43 g/ mL ... number of truncated forms of the protein (Figures 1, 2, 3) Factor Xa cleavage of GP1-MBP 28 60 40 30 20 GP1 17 14 GP2 B 98 62 49 B GP1-MBP MBP 38 28 17 GP1 14 Expression and purification of LASV ... sole members of the Board of Directors of BioFactura, Inc., and have received salary and other compensation from the company, such as founder's stock, as it pertains to the execution of this work...
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báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx
... protein One unit is defined as the amount of enzyme that catalyzes the oxidation of μmol of substrate per minute Results Cloning, expression and purification of 2, 5-diketocamphane 1,2-monooxygenase ... cultivations of the wild type strain P putida NCIMB 10007 with subsequent multiple purification and separation steps of the involved enzymes We report here the first recombinant Figure Operon of the ... though separation of the distinct activities was tried by purification, the presence of impurities could not be excluded Therefore, reproducible and reliable methods for separation and purification...
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purification of some heavy metals from paper making
... distribution characteristics of heavy metals at different parts of the reed parts absorption of Cu (mg/L) absorption of Zn (mg/L) absorption of Pb (mg/L) absorption of Cd (mg/L) root 0.0340+0.0080a ... distribution of four kinds of heavy metals Cu Zn Pb Cd at different parts of the reed Distribution feature of heavy metals in different parts of reed is shown in Table Absorptive capacity of the whole ... This study only considers one kind of wetland plants for the purification of heavy metals from papermaking wastewater But in practice a combination of a variety of plants may be needed to purify...
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báo cáo khoa học: "Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation" pps
... Comparison of purification yield and protein recovery of three Nickel-magnetic matrices for His-ProT and His-Mre11 recombinant proteins Purification yield was defined as the sum of the percents of the ... concentration of beads, the higher fraction of the protein remained attached to the matrix (Figure 5) Effect of bead concentration In order to clarify the effect of bead concentration on the purification ... considerable amount of non-specific proteins eluted in conjunction with this protein Final purity of the purified proteins is without any doubt an excellent measure of the performance of protein purification...
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Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system" docx
... fragment was about 1840 bp 3.2 Expression and purification of polyhistidine-tagged VP2 of PPV We have previously shown that formation of chimeric VLPs of PPV fusion constructs is feasible in pET-PPV ... products purified by IMAC as described below Page of 2.6 Hemagglutination assay Following the method of Senda et al [20], two-fold dilutions of samples of VP2 protein were prepared, mixed with guinea ... rpm) to a concentration of about × 106 cells per ml of culture medium (30 ml growing volume) and infected with the recombinant viruses at a multiplicity of infection (MOI) of 2-3 At 72 h postinfection...
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Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt
... immunodetection of fractions from asialofetuin affinity chromatography Analysis by Analysis by immunodetection of fractions from asialofetuin affinity chromatography Five mg of the glycoprotein asialofetuin ... PBS containing μg of CT adjuvant (Sigma), 15 μg of p24, 30 μg of RTB/p24, 15 μg of p24 mixed with μg of CT and, 30 μg of RTB/p24 mixed with μg of CT adjuvant Intranasal immunizations were performed ... demonstrate the integrity of the protein after purification (Figure 3, panel C) Purification of E coli expressed p24 HIV and chimeric RTB/p24 proteins In order to improve solubilization of the E coli-producedRTB/p24...
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PURIFICATION OF SIMPL ANTIBODY AND IMMUNOFLUORESCENCE OF SIMPL SUB-CELLULAR LOCALIZATION IN RESPONSE TO TNFα- AND IL-1
... protein were being eluted off of the column During previous purifications, all aliquots were pooled, but due to the irregularities of the elution profile for the 087J purification, the aliquots ... column or prevented the purification of antibodies that detected the native structure of SIMPL This wash was the most effective in the elution of detectable protein off of the column during the ... a family of transcription factors that greatly impacts the fate of the cell It can affect the fate of cells in many ways including induction of anti-apoptotic signaling, regulation of cell cycling,...
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studies of pathogenesis-related proteins in the strawberry plant partial purification of a chitinase-containing protein complex and analysis of an osmotin-like protein gene
... sites Binding of Ca++ to these sites caused inhibition of the antifreeze and chitinase activities of these complexes Strawberry is a member of the Rosaceae family, which consists of more than ... report the partial purification of a strawberry protein complex consisting of at least five chitinase isoforms To our knowledge, this protein complex represents the second example of a chitinase-containing ... lane and 3) In view of the existence of chitinasecontaining antifreeze protein complexes in winter rye leaves, a possible explanation of this discrepancy is that one of the isoforms resolved on...
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purification of phytase from aspergillus fumigatus
... 2.2.1 Preparation of fungi strain and culture medium 2.2.2 Extraction of the crude enzyme phytase 2.2.3 Phytase purification 2.2.3.1 Ammonium sulfate fractionation 2.2.3.2 Phytase purification by ... 3.2.1 Ammonium sulfate fractionation 3.2.2 Purification of phytase by hydrophobic interaction chromatography on Phenyl Sepharose 3.2.2 Purification of phytase by cation exchange chromatography ... about the purification of phytase from A fumigatus is available In order to obtain pure enzyme phytase from A fumigatus to apply in feed/food industries, the research on Purification of phytase...
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Optimization of expression and purification of HSPA6 protein from Camelus dromedarius in E. Coli
... Fingure1 Schematic diagram of the hexa-histidine tagged cHSPA6 fusion protein 2.2 2.2.1 Optimization of cHSPA6 overexpression in E Coli Effect of temperature on the overexpression of cHSPA6 Figure showed ... shown in Fig 7b, the level of cHSPA6 expression reached at its maximal level within 1h of induction and incubation at 37 oC The level of cHSPA6 remained unchanged up to 24h of post-induction incubation ... extraction of soluble cHSPA6 The growth of induced culture was ceased approx at OD600 = 2.5 Therefore, approximately 3g wet biomass per liter culture was obtained 2.4 Extraction and purification of cHSPA6...
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