pulmonary langerhans cell histiocytosis lymphangioleiomyomatosis and cryptogenic organizing pneumonia
Báo cáo y học: "Proteome analysis of bronchoalveolar lavage in Pulmonary Langerhans cell histiocytosis" pptx
... smoking controls and Pulmonary Langerhans cell Histiocytosis (PLCH) are visualized by and significant differences occurring between no-smoking controls and Pulmonary Langerhans cell Histiocytosis ... 6(23):6354-69 Aricò M Langerhans cell histiocytosis in adults: more questions than answers? Eur J Cancer 40 (2004); 1467-1473 10 Crausman RS Pulmonary histiocytosis X: pulmonary function and exercise ... controls Plastin triggers immune response, cell migration, proliferation and cell- adhesion (25) and its role in actin cytoskeleton rearrangement and T -cell activation is crucial Another function...
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Báo cáo khoa học: "A rare case of “switch on and off” multi-system Langerhans cell histiocytosis in an adult patient" doc
... Vassallo R, Ryu JH, Colby TV, Hartman T, Limper AH: Pulmonary Langerhans -cell histiocytosis N Engl J Med 2000, 342:1969-1978 Arico M: Langerhans cell histiocytosis in adults: more questions than answers? ... Pneumothorax in pulmonary Langerhans cell histiocytosis Chest 2004, 125:1028-1032 doi:10.1186/1752-1947-5-302 Cite this article as: Karpathiou et al.: A rare case of “switch on and off” multi-system Langerhans ... Radiography and (B) bone scan of the right humerus at initial diagnosis showing Langerhans cell histiocytosis localization on the one-third medium which resolved (C and D) at the time of pulmonary...
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Báo cáo y học: "Langerhans cell histiocytosis involving the liver of a male smoker: a case report" pot
... Follath F: Langerhans' -cell histiocytosis in adults Med Pediatr Oncol 1997, 28(1):9-14 Islinger RB, Kuklo TR, Owens BD, Horan PJ, Choma TJ, Murphey MD, Temple HT: Langerhans' cell histiocytosis ... JJ: Pulmonary Langerhans' cell histiocytosis: radiologic resolution following smoking cessation Chest 1999, 115(5):1452-1455 Vassallo R, Ryu JH, Colby TV, Hartman T, Limper AH: Pulmonary Langerhans' -cell ... HIV and hepatitis testing were negative Coagulation, hepatic function and urine sediment were unremarkable Bronchofibroscopy and bronchoalveolar lavage were negative for malignant cells, and...
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Báo cáo y học: " Augmentation of Pulmonary Epithelial Cell IL-8 Expression and Permeability by Pre-B-cell Colony Enhancing Factor" docx
... expression on in vitro cell permeability of Effectscells and HPAEC Effects of PBEF expression on in vitro cell permeability of A549 cells and HPAEC A549 cells or HPAEC cells were seeded onto ... expression affected cell permeability in A549 cells and human pulmonary artery endothelial cells (HPAEC) To assess whether PBEF expression affected lung alveolar epithelial cell and HPAEC permeability, ... induced cell permeability in both A549 and HPAEC cells (Figure 7A and 7C) Even under baseline (TNFa) conditions, overexpressing PBEF significantly increased cell permeability in A549 cells (over-expression...
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Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor
... for controlling cell cycle progression (Morgan 1997; Zachariae and Nasmyth 1999) These studies and others demonstrated the universality of the cell Modeling Cell Cycle Control and Cancer with ... diabetes and Cdk4 activation results in betaislet cell hyperplasia Nat Genet 22:44–52 Reed SI (2003) Ratchets and clocks: the cell cycle, ubiquitylation and protein turnover Nat Rev Mol Cell Biol ... Cdc28 and classes of vertebrate cyclins (D- and E-type cyclins in G1 and A -and B-type cyclins in G2/M) that control a family of Cdks (cyclindependent kinases) greatly enhanced our understanding...
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The Retinoblastoma Gene Family in Cell Cycle Regulation and Suppression of Tumorigenesis
... most cell cycle responses While each single pocket protein can mediate cell cycle arrest upon cell- cell contact, pRb is the critical mediator of cell cycle arrest upon growth factor deprivation and ... (Rod/cone photoreceptor cells in ONL) pituitary gland tumors (Rod/cone photoreceptor cells in ONL) pituitary gland tumors (Rod/cone photoreceptor cells in ONL) pituitary gland tumors, pinealoblastoma ... delayed cell cycle exit of double knockout cells may indicate that oncogenic signals can induce a bypass of differentiation more easily in cells that lack both Rb and p107 or Rb and p130 In cells...
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Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf
... promotes growth activity of MGC803 cells MGC803 cells were transfected with miR-23a ASO or pri-23a vector, and cell growth activity was detected through the MTT (A) and colony formation (B) assays ... gastric cancer growth L.-H Zhu et al Cell culture and transfection Human gastric adenocarcinoma cell line MGC803 was maintained in RPMI1640 (GIBCO BRL, Grand Island, NY, USA) supplemented with 10% ... Acquisition and Analysis Software (UVP, Upland, CA, USA) was used to quantify band intensities All primers were purchased from AuGCT Inc MTT assay MGC803 cells were seeded in 96-well plates with · 103 cells...
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Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
... easier handling of the cells, treatment was performed on HeLa cells instead of CHO K1 cells Cells were then incubated with of lgámL)1 Tat±GFP fusion protein or with lM ¯uorescein Tat peptide and ... was observed for all cell lines (Fig 2A) In order to minimize cell handling prior to FACS analysis, no ®xation step was included Avoiding cell ®xation and working on living cells eliminates potential ... use Cells and cell cultures HeLa cells were cultured as exponentially growing subcon¯uent monolayers on 90-mm plates in RPMI 1640 medium (Gibco) supplemented with 10% (v/v) fetal bovine serum and...
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A COMPARATIVE STUDY OF THE DIAGNOSIS OF PULMONARY TUBERCULOSIS USING CONVENTIONAL TOOLS AND POLYMERASE CHAIN REACTION* pot
... serial-c) These cases were as follows, two each of asthma, pneumonia and cancer lung, one each of post CABG, COPD and lung abscess and 12 with cough and cold All samples from these individuals had tested ... Tuberculosis (MOTT), identified as M scrofulaceum and M intracellulare PCR and microscopy were negative for both samples identified as MOTT and positive for 98% (47/48) of the remaining samples ... like to thank Dr A K Chakraborty and the anonymous reviewers for comments and suggestions on the manuscript, Dr N S Deodhar for comments and suggestion on the work and Drs Anil Ravetkar, former...
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Báo cáo khoa học: Cell biology, regulation and inhibition of b-secretase (BACE-1) potx
... isophthalamide derivatives as the P2–P3 ligands showed good selectivity between BACE-1 and BACE-2, nanomolar potency in vitro and in cell- based studies, and a significant reduction in Ab40 levels ... 10001–10006 110 Andrau D, Dumanchin-Njock C, Ayral E, Vizzavona J, Farzan M, Boisbrun M, Fulcrand P, Hernandez JF, Martinez J, Lefranc-Jullien S et al (2003) BACE1- and BACE2-expressing human cells: characterization ... FEBS 1849 Biology and chemistry of b-secretase (BACE-1) C E Hunt and A J Turner BACE-1 promoter, and several studies have reported the upregulation of BACE-1 mRNA both in vitro and in vivo following...
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Báo cáo khoa học: Programmed cell death Apoptosis and alternative deathstyles pot
... induced caspaseindependent cell death No DNA fragmentation was observed and dying cells showed neither condensation nor fragmentation of cells and nuclei, but the cells and nuclei swelled in a manner ... autophagy and caspase-dependent cell death pathways Camougrand and coworkers [133] showed in yeast that the expression of Bax induces cell death with characteristics of both apoptosis and autophagy, ... SAPK/JNK and p38, and increased a-synuclein expression Both cell viability and the increase in a-synuclein expression were prevented by antioxidants, by the specific inhibitors of p38 and SAPK/JNK, and...
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Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
... tumor cell lines and compare them to primary cells Cellular lysates were prepared from human foreskin fibroblasts, a primary cell line, and the tumorigenic cell lines Saos, MDAMB231, MCF7, HeLa and ... geminin and actin (as loading control) are shown in serum deprived NIH3T3 cells, and and 12 h following serum re-addition, and compared with mRNA levels in proliferating cells Both Cdt1 and geminin ... sections and their expression levels in primary and normal diploid vs cancer cell lines Our data show a correlation of Cdt1 and geminin expression levels with cell proliferation Materials and methods...
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Diagnosis of pulmonary tuberculosis by smear microscopy and culture in a tertiary health care facility: Biology and Medicine docx
... Sensitivity and Specificity was found to be 93.45% and 88.10 % for Primary and secondary smear respectively The Positive predictive value and Negative Predictive value was 96.91 % and 77.08 % ... clinical and radiological presentations were enrolled Old cases of Pulmonary Tuberculosis were also included in our study The clinical criteria and patient history was taken Children and cases ... gold standard With the emergence of MDR and XDR TB, case detection and identification retains a very high priority With this study we wish to emphasize the efficiency of ZN staining method and Culture...
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Báo cáo khoa học: Plant oxylipins: role of jasmonic acid during programmed cell death, defence and leaf senescence doc
... of EDS1 ⁄ PAD4 ⁄ SAG101 and BON1 ⁄ BAP1 + in cell death and plant pathogen resistance Whereas EDS1, PAD4 and SAG101 are positive regulators of cell death, BON1, BAP1 and BAP2 operate as negative ... senescence-associated and senescence-induced kinases, called SARK and SIRK, respectively [125] SARK and SIRK share similar structures and consist of an extracellular leucine-rich domain, a transmembrane domain, and ... and JA-mediated cell death in irradiated flu plants is likely to be a form of programmed cell death (PCD) [29] In many aspects it resembles PCD and apoptosis in animals [95–99] This includes cell...
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Báo cáo khoa học: Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos pot
... A549 cells, H1299 and monkey Cos-1 cells has been also reported [34] Tat-eGFP and VP-22 linked N-terminus of diptheria toxin A fragment have shown restricted translocation in muscle and Vero cells, ... mechanism of cell entry of CPPs [15], which may also involve extracellular heparan sulfate and various endocytosis and macropinocytosis pathways [16–23] It is also suggested that classical and nonclassical ... Madin–Darby canine kidney renal epithelial cells and CaCo-2 colonic carcinoma cells has been achieved by treatment with the cell membrane permeabilizing agent digitonin and acetone ⁄ methanol [32] The effect...
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Báo cáo khoa học: Extraenzymatic functions of the dipeptidyl peptidase IV-related proteins DP8 and DP9 in cell adhesion, migration and apoptosis doc
... Table 1, and DP8 was assessed by cell stain (not shown) DP8 and DP9 have been localized to Golgi and endoplasmic reticulum [7,8] Concordantly, in the 293T cells transfected with DP8–GFP and DP9–GFP, ... and DP9 overexpression to impair monolayer wound healing on ECM DP8 and DP9 impaired cell migration Cell migration was also assessed in transwells In vitro cell migration assays showed that cells ... in vitro wound healing, cell migration and apoptosis, and for DP8 in wound healing, cell migration and apoptosis enhancement (Table 4) DP9 overexpression impaired 2452 cell behavior with regard...
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Báo cáo khóa học: High level cell-free expression and specific labeling of integral membrane proteins doc
... bromide (2.5 lM) 16 and nigericine (5 lgÆmL)1) (Sigma) Green fluorescent protein (GFP) fluorescence was measured at excitation and emission wavelengths of 395 and 509 nm, and at 474 and 509 nm for the ... the cell- free system Soluble GFP production in a standard cell- free reaction with a membrane cut-off of 25 kDa and an RM/FM ratio of : 17 was monitored by fluorescence at an emission at 509 nm and ... after standard cell- free expressions Modification of the cell- free expression system by addition of detergents and lipids The results obtained with the EmrE-sgGFP fusion gave evidence that a cell- free...
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cancer cell culture, methods and protocols
... A and Flandermeyer, R R (1977) Inter- and intraspecies contamination of human breast tumor cell lines HBC and BrCa5 and other cell cultures Science 195, 1343–1344 Gey, G O., Coffman, W D., and ... 1.2 Routine Feeding and Maintenance Cell cultures should be examined regularly and routinely (preferably daily) both macroscopically and microscopically The cell morphology and cell density should ... give a single cell suspension is often referred to as “harvesting.” The use of proteolytic enzymes such as trypsin, breaks the cell- cell and cell- substrate links and creates a single -cell suspension...
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cancer cell signaling methods and protocols
... instead of feeder cells for a short period, particularly when we needed pure ES cells without feeder cells for biochemical analysis etc (see Note 1) 2.1.1 Cells R1 cells and STO cells were kindly ... Stock Thaw cells at 37ºC and wash once with medium Add mL of medium and pipeting Transfer cells into T-25 flask with feeder cells and culture at 37ºC, 5% CO2 Change medium at d or d and passage ... trypsin/EDTA and incubate for 10 at 37ºC Pipet the cells and transfer to a 24-well plate with feeder cells and mL of medium After the cells have grown to 50% confluence, wash once with PBS(-) and incubate...
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cell cycle checkpoints and cancer
... blood, activated T cells, natural killer cells, mast cells and some megakaryocytic cells can synthesize IL-3.17-19 For optimal IL-3 expression, T cells must be activated via the T -cell receptor (TCR)/CD3 ... 1-58706-067-1 (alk paper) Cancer cells Regulation Cell cycle Cellular signal transduction Cell transformation Carcinogenesis [DNLM: Cell Transformation, Neoplastic Cell Cycle QZ 202 C39253 2001] ... Protein and mRNA during the Cell Cycle 126 Subcellular Localization 127 Activity at Cell Cycle Checkpoints 129 Interactions with Cell Cycle Proteins 130 Transcription of Cell...
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