pulmonary epithelial cell targeting
Báo cáo y học: " Augmentation of Pulmonary Epithelial Cell IL-8 Expression and Permeability by Pre-B-cell Colony Enhancing Factor" docx
... PBEF may have an important role in epithelial cell barrier regulation A549 cells are an alveolar Type II tumor epithelial cell line While the type II epithelial cell only covers 7% of the alveolar ... expression on in vitro cell permeability of Effectscells and HPAEC Effects of PBEF expression on in vitro cell permeability of A549 cells and HPAEC A549 cells or HPAEC cells were seeded onto ... expression affected cell permeability in A549 cells and human pulmonary artery endothelial cells (HPAEC) To assess whether PBEF expression affected lung alveolar epithelial cell and HPAEC permeability,...
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epithelial cell culture protocols
... many types of cells, including smooth muscle cells, mesenchymal cells, vessel forming cells, nerve cells, blood cells, including immune cells and gastric gland cells Gastric epithelial cells can ... Cell Culture 1 Human Lens Epithelial Cell Culture Nobuhiro Ibaraki Introduction Crystalline lens consists of epithelial cells, fiber cells, and a capsule They originate from lens epithelial cells ... electrical properties or enzyme production of epithelial cell sheets or transepithelial permeability, plate cells at 106 viable cells/cm2 onto MillicellCM or Millicell-HA inserts coated with Vitrogen...
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Báo cáo sinh học: " Kinetics of antibody-induced modulation of respiratory syncytial virus antigens in a human epithelial cell line" docx
... immunofluorescence of RSV antigen in infected epithelial cells Indirect Indirect immunofluorescence of RSV antigen in infected epithelial cells Viral proteins in HEp-2 cells, which had been infected at m.o.i ... hypertonic medium Kinetics intracellular RSV proteins in cells incubated in Kinetics of intracellular RSV proteins in cells incubated in hypertonic medium HEp-2 cells had been infected with anti-RSV ... infected cell modify cellsurface viral determinants, the intracellular viral polypeptide concentration and interfere with viral induced destruction of the cell Alterations of RSV intracellular...
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Báo cáo hóa học: " Kinetics of antibody-induced modulation of respiratory syncytial virus antigens in a human epithelial cell line" pot
... immunofluorescence of RSV antigen in infected epithelial cells Indirect Indirect immunofluorescence of RSV antigen in infected epithelial cells Viral proteins in HEp-2 cells, which had been infected at m.o.i ... hypertonic medium Kinetics intracellular RSV proteins in cells incubated in Kinetics of intracellular RSV proteins in cells incubated in hypertonic medium HEp-2 cells had been infected with anti-RSV ... infected cell modify cellsurface viral determinants, the intracellular viral polypeptide concentration and interfere with viral induced destruction of the cell Alterations of RSV intracellular...
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Báo cáo hóa học: " Respiratory syncytial virus glycoproteins uptake occurs through clathrin-mediated endocytosis in a human epithelial cell line" docx
... envelope proteins in Hep-2 cells Endocytosis of RSV envelope proteins in Hep-2 cells Hep-2 cells were infected with RSV at a multiplicity of infection of After 12 h, the cells were incubated with ... microscopy For this purpose, HEp-2 cells were RSV infected for 12 h Origin of cells, virus propagation and infection procedures were previously reported [8] The infected cells were washed and incubated ... mechanism [8] With the aim to confirm whether internalization of RSV cell surface antigen-antibody complexes in epithelial cells occurs through clathrin, the present study was undertaken The uptake...
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Báo cáo hóa học: " Characteristics of functionalized nanohydroxyapatite and internalization by human epithelial cell" pdf
... shown) Cell toxicity of Arg-Eu-HAP The effect of varying concentrations and exposure time of Arg-Eu-HAP on cell toxicity was evaluated using human epithelial lung cancer cell line (A549) The cell ... instructions were followed Cell binding and cellular internalization of Arg-Eu-HAP To track the internalization of Arg-Eu-HAP, A549 cells were seeded in 12-well plates at × 105 cells per well and incubated ... affect its interaction with cells [28,29] We performed uptake studies in cell culture medium with free serum Cellular uptake of Arg-Eu-HAP was investigated in A549 cell line In order to visualize...
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Báo cáo y học: "Proteome analysis of bronchoalveolar lavage in Pulmonary Langerhans cell histiocytosis" pptx
... Respir Cell Mol Biol 2008;38(6):655-60 Soler P, Moreau A, Basset F, Hance AJ Cigarette smoking-induced changes in the number and differentiated state of pulmonary dendritic cells/Langerhans cells ... smoking controls and Pulmonary Langerhans cell Histiocytosis (PLCH) are visualized by ¥and significant differences occurring between no-smoking controls and Pulmonary Langerhans cell Histiocytosis ... controls Plastin triggers immune response, cell migration, proliferation and cell- adhesion (25) and its role in actin cytoskeleton rearrangement and T -cell activation is crucial Another function...
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Báo cáo khoa hoc:" Diffuse idiopathic pulmonary neuroendocrine cell hyperplasia (DIPNECH) in association with an adenocarcinoma: a case report" ppsx
... Castro CY: Diffuse idiopathic pulmonary neuroendocrine cell hyperplasia Ann Diagn Pathol 2007, 11:122-126 Gosney JR, Travis WD: Diffuse idiopathic pulmonary neuroendocrine cell hyperplasia In Tumours ... idiopathic pulmonary neuroendocrine cell hyperplasia and a typical carcinoid tumor J Thorac Cardiovasc Surg 2006, 131:1207-1208 Gosney JR: Diffuse idiopathic pulmonary neuroendocrine cell hyperplasia ... idiopathic pulmonary neuroendocrine cell hyperplasia (DIPNECH; B; primary magnification ×10) Conclusion DIPNECH is a rare disorder and it is considered to be a precursor lesion for pulmonary carcinoid...
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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
... monocytes, eosinophils, basophils, and CD4+ T cells [45] CCL-5/RANTES production from bronchial epithelial cells contributes to infiltration of inflammatory cells in the airway during viral infection ... protein concentrations of cytokines/chemokines in cell culture supernatants were measured (Figure 3, Table 3) The results showed that the epithelial cells secreted high amounts of IL-6, IL-8, CXCL-10/IP-10, ... proliferation and differentiation of B cells, and proliferation of T cells As a result, all these TNF-a activated mediators could contribute to the infiltration of inflammatory cells into the influenza infected...
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Báo cáo y học: " KL-6 concentration in pulmonary epithelial lining fluid is a useful prognostic indicator in patients with acute respiratory distress syndrome" ppt
... the epithelial cell membrane, KL-6 can diffuse into pulmonary epithelial lining fluid (ELF) In the normal lung, this glycoprotein can be predominantly found on alveolar type II epithelial cells, ... II cells can proliferate when alveolar epithelial cell damage is mild or moderate, but when the damage is very severe, even type II cells cannot survive and are replaced by the epithelial cells ... alveolar type II cells following injury to alveolar type I cells [21], and therefore its level must reflect the severity of alveolar epithelial injury The degree of alveolar epithelial injury...
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Báo cáo y học: " Differentiated transplant derived airway epithelial cell cytokine secretion is not regulated by cyclosporine" pdf
... basal cells, Mucin 5AC (clone C-20, Santa Cruz Biotechnology, Santa Cruz CA) binding goblet cells and b-tubulin (catalogue # ab6046, Abcam Inc., Cambridge, MA) marking ciliated cells Epithelial cell ... (ng/ml) A † 100 50 Vehicle 150 Figure Pulmonary allograft epithelial cells in culture A Phase-contrast image of cells in submersion culture B Phasecontrast image of cells in air liquid interface culture ... Confocal microscopy of air liquid interface cells at weeks Cells were labeled with antibodies directed against ciliated cells (blue), goblet cells (red), or basal cells (green) White represents the overlap...
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Báo cáo y học: " Epithelial cell senescence impairs repair process and exacerbates inflammation after airway injury" doc
... CC10-positive cells by the total number of CC10-positive cells, or the number of Ki-67-labeled nuclei in the CC10-negative epithelial cells by the total number of CC10-negative epithelial cells Epithelial ... CC10-positive epithelial cells that consists of a pollutant-resistant subpopulation of Clara cells that retain expression of CC10 (variant CC10/CCSP-expressing cells; vCE cells), bronchoalveolar stem cells ... of the epithelial cell exfoliation into the airway lumen (Figure 1B) or reduction and subsequent recovery in the number of Clara cell 10-kDa secretory protein (CC10)-positive cells (Clara cells)...
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Báo cáo y học: " Upregulation of pirin expression by chronic cigarette smoking is associated with bronchial epithelial cell apoptosis" ppsx
... primary human bronchial epithelial cells were exposed to cigarette smoke extract in vitro Human bronchial epithelial cells were used, as they most closely mimic airway epithelial cells in their natural ... to the epithelial cell itself, and to a lesser extent, disruption in the tight junctions between epithelial cells are partly responsible [10,11] Consistent with the bronchial epithelial cell studies, ... cigarette smoke extract (CSE) in a variety of cell types, including airway epithelial cells, alveolar macrophages, endothelial cells, alveolar epithelial cells, and fibroblasts [13-15] In contrast,...
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Báo cáo y học: " The role of IFN-γ in regulation of IFN-γ-inducible protein 10 (IP-10) expression in lung epithelial cell and peripheral blood mononuclear cell co-cultures" potx
... two human lung epithelial cell lines, A549 (alveolar epithelial cell line), Calu-3 (bronchial epithelial cell line) in addition to primary normal human bronchial epithelial (NHBE) cells Materials ... of human epithelial cell lines Cells from a human bronchial epithelial cell line (Calu-3) and from a human alveolar epithelial cell line (A549) were used for the present studies Both cell lines ... many cell types including lung epithelial cells [13,5,14] and it has been shown to be involved in epithelial cell movement via p38 MAPK and PI3K dependent signalling pathways in human airway epithelial...
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Báo cáo y học: " Protection of pulmonary epithelial cells from oxidative stress by hMYH adenine glycosylase" pptx
... carcinoma cellline, which likely differs both in proliferative properties as well as in response to oxidative stress in comparison to primary epithelial cells The enhanced cell growth observed with cell ... to in vivo pulmonary cells Conclusions In summary, we have demonstrated that over-expression of the DNA glycosylase repair enzyme hMYH may enhance survival of a pulmonary epithelial cell line ... Four days after exposure, cells were fixed and assessed for cell growth/survival by the SRB assay LTR Bam HI pSF91.1 EcoRI SalI Western Analysis Cell pellets of sorted cells were resuspended in...
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Báo cáo y học: "Melatonin promoted chemotaxins expression in lung epithelial cell stimulated with TNF-α" docx
... obtained from all subjects in the study Cell Culture A549 cells, human type II-like epithelial lung cells, were obtained from ATCC (Manassas, VA, USA) The cells were cultured in tissue flasks incubated ... TNF-α is expressed primarily by the alveolar cells and tissue macrophages, mast cells, and bronchial epithelial cells Additionally, in most other airway cell systems studied, conditions simulating ... Activation Normal T-cells Expressed and Secreted) are C-C chemotaxins that can recruit eosinophils to the airway in asthma[4] A variety of tissues and cell types, including lung epithelial cell, produce...
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Báo cáo y học: " Airway epithelial cell tolerance to Pseudomonas aeruginosa" pps
... understanding tolerance mechanisms in airway epithelial cells, we studied AALEB cells, a novel SV40 and telomerase immortalized hTBE derived cell line [42] AALEB cells on plastic detached from the culture ... lipid rafts at the apical epithelial cell surface [15] TLR1-10 expression and positive responses to several TLR agonists were recently reported in airway epithelial cells[16] Thus, the TLR signal ... to be heat-resistant Cell culture Under an Institutional Review Board-approved protocol, hTBE cell cultures were prepared as previously described[41] Briefly, epithelial cells were removed from...
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Báo cáo y học: "Atorvastatin reduces lipopolysaccharide-induced expression of cyclooxygenase-2 in human pulmonary epithelial cells" ppsx
... COX2 in cultured vascular smooth muscle cells [4] However, it is not clear whether Atorvastatin also affects COX-2 expression in human pulmonary epithelial cells Because of importance of COX-2 in ... smooth muscle cells Atherosclerosis 2002, 160:49-58 Mitchell JA, Belvisi MG, Akarasereenont P, et al.: Induction of cyclooxygenase-2 by cytokines in human pulmonary epithelial cells: regulation ... using the ImageQuant densitometer (Gel Doc 2000, BioRad Co) Methods 2.1 Materials Human pulmonary epithelial cell line (A549) was purchased from American Type Culture Collection (ATCC) Medium DMEM,...
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Báo cáo y học: " Alveolar macrophage-epithelial cell interaction following exposure to atmospheric particles induces the release of mediators involved in monocyte mobilization and recruitment" pot
... [4-7] and epithelial [7-10] and other lung cells [11] When AM and airway epithelial cells are directly exposed to inhaled atmospheric particles these small particles are phagocytized by both cells ... Then the cells were trypsinized and placed in 100-mm cell culture dishes and cultured in BEGM Light microscopy showed that 95% of the isolated cells had features of bronchial epithelial cells, ... Exposure of cells to PM10 Primary HBEC from the third or fourth passage of cells from each patient were cultured to 90–100 % confluence in 100-mm cell culture dishes (approximately 2.5–3.0 × 106 cells/dish)...
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Báo cáo khoa học: "The early responses of VEGF and its receptors during acute lung injury: implication of VEGF in alveolar epithelial cell survival" ppt
... lung epithelial cells Knock-down of either VEGF or VEGFR-1 reduced lung epithelial cell viability To further determine the roles of VEGF and VEGFR-1 in lung epithelial cell survival, human lung epithelial ... VEGF-positive cells were clearly decreased, especially in alveolar epithelial cells and bronchial epithelial cells (Figure 2c, Table 2) The percentage of identifiable VEGF-positive cells in the ... Relationship between TUNEL-positive -epithelial cells and VEGF-positive cells (d) Relationship between TUNEL-positive -epithelial cells and VEGFR-1-positive cells however, is largely unknown Recent...
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