ptdins 3 p binding proteins in endosomal membrane trafficking
Tài liệu Báo cáo Y học: Systematic search for zinc-binding proteins in Escherichia coli potx
... Zn(II) -binding proteins The specificity of Zn(II) binding was also examined for the purified Zn(II) -binding proteins The binding of radioactive Zn(II) to AckA, Def, DnaJ, Fur, MerR, Ppa, RplM, and RpoA ... represents the specific binding of Zn(II) Fig Dose-dependent binding of Zn(II) to purified E coli zinc -binding proteins The newly identified E coli Zn(II) -binding proteins, AckA and Ppa, were analysed, ... (ribosomal protein L 13) and RpoA (RNA polymerase a subunit), in parallel with four known heavy metal -binding proteins, Zn(II) -binding Def (peptide deformylase) [22, 23] , Hg(II) -binding MerR (mercury export...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
... EP (19 93) Orientation and topography Actin and ABPs in transcription regulation 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 of RNA polymerase III in transcription complexes Mol Cell Biol 13, ... nuclear actin -binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand -binding domain Actin -binding protein Targeting sequence Gelsolin Direct or indirect association ... (g) actin is a component of pre-mRNP particles, and is incorporated into pre-mRNAs by binding to a specific subset of RNA -binding proteins [4,22]; and (h) actin is a component of PICs and depletion...
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Báo cáo Y học: Co-existence of two regulatory NADP-glyceraldehyde 3-P dehydrogenase complexes in higher plant chloroplasts ppt
... In this paper, we have attempted to characterize such a PRK-containing complex, having in mind the postulated existence of a mixed GAPDH–PRK complex containing CP12 as described previously [33 ] ... lg 1 ,3- bisphosphoglycerate (+ DTT/1,3bisPGA) DTT, dithiothreitol Table Percentage coprecipitation of PRK-, GAPDH- and CP12containing complexes from oxidized stroma The activities remaining in the ... 96.1% of the PRK This again indicates the presence of an independent GAPDH-A8B8 complex apart from the PRK/GAPDH/CP12-mixed complex The fact that the antiserum raised against recombinant CP12 only...
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báo cáo hóa học:" Assessing the clinical utility of measuring Insulin-like Growth Factor Binding Proteins in tissues and sera of melanoma patients" potx
... Insulin-like Growth Factor-1 receptor; (IGFBP -3) : Insulin-like Growth Factor Binding Protein -3; (IGFBP-4): Insulin-like Growth Factor Binding; Protein-4; (MAPK): Mitogen-activated protein kinase; ... (56.7) 34 (56.7) 26 ( 43. 3) 26 ( 43. 3) 34 (56.7) 20 (33 .3) 26 ( 43. 3) (3. 33) (10.0) (10.0) IGFBPs are produced and/or degraded differ between the tumor microenvironment and plasma, leading to increases ... apoptosis through pathways mediated by the secreted protein IGFBP7 Cell 2008, 132 (3) :36 3-74 Durai R, Yang SY, Sales KM, Seifalian AM, Goldspink G, Winslet MC: Insulin-like growth factor binding...
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báo cáo khoa học: " The Puf family of RNA-binding proteins in plants: phylogeny, structural modeling, activity and subcellular localization" potx
... (AtPum7-GFP:AtPum18-RFP, AtPum9-GFP:AtPum18RFP, AtPum12-GFP:AtPum18-RFP, AtPum14-GFP: AtPum18-RFP, AtPum10-GFP:AtPum8-RFP) were coexpressed in epidermal cells Each pair of co-expressed AtPum ... exon-junction complex of proteins, RNA -binding proteins, and other proteins localize to nucleoli in plant cells supports a role for the nucleolus in mRNA processing, silencing, surveillance and export [44,45] ... mitochondrial biogenesis: a role for the PUF RNA -binding protein Puf 3p in mRNA localization PLoS ONE 2008, 3( 6):e22 93 Spassov DS, Jurecic R: The PUF family of RNA -binding proteins: does evolutionarily conserved...
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Molecular analysis of oxysterol binding proteins in yeast
... Ethanolamine; PH: Pleckstrin Homology; PIK1: yeast PhosphatidylInositol 4-Kinase; PI /PtdIns: PhosphatidylInositides; PIP: PhosphatidylInositol Phosphate or Phosphoinositides; PLO: Protein Lipid Overlay ... cerevisiae; PA/PtdOH: Phosphatidic Acid; PAGE: PolyAcrylmide Gel Electrophoresis; PBS: Phosphate Buffered Saline; PC/PtdCho: PhosphatidylCholine, PCR: Polymerase Chain Reaction; PE/PtdEth: Phosphatidyl ... Oxysterol Binding domain; O.D: Optical Density; OPI1: OverProduction of Inositol; ORD: OSBP Related Domain; ORP: OSBP Related Protein in humans; OSBP: Oxysterol Binding Protein; OSH: OSBP Homologues in...
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Báo cáo khoa học: A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2 pptx
... phosphatidylinositol 3, 4-bisphosphate [PtdIns( 3, 4 )P2 ], PtdIns( 3, 5 )P2 , PtdIns( 4,5 )P2 and phosphatidylinositol 3, 4,5-triphosphate [PtdIns( 3, 4,5 )P3 ], and weakly with PtdIns( 3) P, PtdIns( 4 )P and PtdIns( 5 )P ... 5-monophosphate; PtdIns( 3, 4 )P2 , phosphatidylinositol 3, 4-bisphosphate; PtdIns( 3, 5 )P2 , phosphatidylinositol 3, 5-bisphosphate; PtdIns( 4,5 )P2 , phosphatidylinositol 4,5-bisphosphate; PtdIns( 3, 4,5 )P3 , phosphatidylinositol ... crystallographic study needs to be performed to determine the binding site In plants, PtdIns( 3) P, PtdIns( 4 )P, PtdIns( 5 )P, PtdIns( 3, 4 )P2 , PtdIns( 3, 5 )P2 and PtdIns( 4,5 )P2 have been detected In contrast, PtdIns( 3, 4,5 )P3 ...
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Tài liệu Báo cáo khoa học: Disulfide bridge regulates ligand-binding site selectivity in liver bile acid-binding proteins ppt
... lead to distinct binding and functional properties [22, 23] In line with this, we have shown here that the presence of a disulfide bridge, while maintaining the same binding stoichiometry, induces ... values in the apo protein and in all of the investigated holo proteins, thus plays a key role in regulating the positioning of the helix–loop–helix motif with respect to the b-barrel in order ... determinants of cooperativity in the liver bile acid binding protein Proteins Struct Funct Bioinformatics doi:10.1002/prot.22496 Tochtrop GP, Bruns JL, Tang C, Covey DF & Cistola DP (20 03) Steroid...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... sequence-nonspecific preferential binding to globally cis-platinated DNA [31 ,33 ,34 ] Binding of p 73 proteins to the recognition elements is affected by DNA cis-platination in a similar way to p 53 binding In agreement ... described previously [18 ,33 ] Proteins p7 3b and p7 3d were prepared using the TNTÒ Quick Coupled Transcription ⁄ Translation System (Promega) Plasmids pcDNA3HA -p7 3b or pcDNA3-HA -p7 3d (1 lg) coding the ... resulted in a decrease of p 53 binding to the p2 1 target [in agreement with formation of the adducts within both p2 1 and pPGM1 p5 3DBSs; see (i)] In contrast, apparent p 53 binding to the p2 1b target increased...
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Tài liệu Báo cáo khoa học: Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae docx
... aqueous phase containing peripheral membrane proteins, a detergent-phase in which proteins with a-helical transmembrane domains are soluble and a ‘phospholipid-enriched’ pellet fraction containing proteins ... nuclearencoded preproteins into the mitochondrial outer membrane Bioessays 22, 36 4 37 1 13 Rapaport D (20 03) Finding the right organelle: targeting signals in mitochondrial outer -membrane proteins EMBO Rep ... 276, 32 399 32 402 Integral proteins in the mitochondrial outer membrane Tokuda H & Matsuyama S (2004) Sorting of lipoproteins to the outer membrane in E coli Biochim Biophys Acta 16 93, 5– 13 Bos MP...
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Tài liệu Báo cáo khóa học: A multi-protein complex containing cold shock domain (Y-box) and polypyrimidine tract binding proteins forms on the vascular endothelial growth factor mRNA Potential role in mRNA stabilization pptx
... of partner proteins in solution [22, 23] Involvement of the VEGF 3 -UTR in binding CSD and PTB proteins As sequences in the 3 -UTR are also involved in posttranscriptional regulation of VEGF expression, ... and PTB (3 ) protein binding sites are underlined Both sequences are required for cytoplasmic CSD/PTB complex formation and recombinant CSD protein binding (B) A comparison of CSD protein RNA binding ... roles CSD and PTB proteins may play in directing CCV1 complex binding to the 5¢-ACCUCUU -3 and 5¢-UUUUCUU -3 sequences, recombinant CSD (GST-dbpB/YB-1) and PTB (GSTPTB) binding to wild-type (V1) and...
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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf
... 42 .38 32 . 93 32.75 43. 26 39 .79 42 .38 29. 63 27.04 20.69 18.15 5.8 5.2 5.2 5.2 4.7 4.7 6.6 8.8 5.2 6.9 6.9 5.0 4.1 P0 7725 P9 9041 P3 1001 P9 9041 P5 8774 P5 8771 P0 731 0 Q9CP09 P9 9041 P1 6015 P1 7751 P0 5977 ... freshly prepared protease inhibitor cocktail (0.2 mM pefabloc, 1.4 lM pepstatin, 0.15 lM aprotinin, 0 .3 lM E-64, lM leupetin, 0.5 mM soybean trypsin inhibitor and mM EDTA) [33 ] In order to eliminate ... calsequestrin-like proteins and impaired calcium binding in dystrophic mdx muscle J Appl Physiol 92, 435 –445 30 Dowling, P. , Doran, P & Ohlendieck, K (2004) Drastic reduction of sarcalumenin in Dp427-deficient...
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Báo cáo khoa học: Proteomic and biochemical analysis of 14-3-3-binding proteins during C2-ceramide-induced apoptosis pot
... Proteins that bind to the phosphopeptide -binding site of 14 -3- 3 proteins were 14 -3- 3 -binding status during apoptosis eluted with 12 mL of mm ARAApSAPA phosphopeptide in buffer A Samples (12 mL) ... isolated proteins bind to the phosphopeptide -binding sites on the 14 -3- 3 proteins, either directly or as components of protein complexes As mentioned above, 14 -3- 3 interacts with apoptosis-related proteins ... ⁄ HCl (pH 7.4) The 14 -3- 3 -binding proteins were eluted using a phosphopeptide (ARAApSAPA) that competes with proteins for the 14 -3- 3 binding Eluted pools were then subjected to electrophoresis...
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Báo cáo khoa học: Pherokine-2 and -3 Two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections ppt
... as the retinol -binding protein and the cholesterol -binding protein apoD [42], or of RYA3, which exhibits significant sequence homology to the LPS -binding protein [ 43] This latter example finally ... observed in white pupae (0–72 h) Expression decreases in black pupae (72–96 h) and adults phk-2 is also expressed in larvae and pupae, with a strong peak of expression in black pupae Both phk-2 and phk -3 ... bacteria [38 ] Pherokines may be involved in a similar type of chemosensory behavior in Drosophila Another interesting possibility is that pherokines may participate in the control of reproduction in...
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Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt
... (1.95–1.90) P2 12121 a = 60 .33 b = 65 .39 c = 96.49 a = b = c = 90° 0.052 (0.21) 13. 8 (3. 3) 98.9 (95 .3) 3. 3 (2.6) 30 432 0. 135 0.168 0.014 1 .35 0.09 32 11 38 . 23 2.00 (2.11–2.00) P2 12121 a = 58.57 b = 64. 63 ... 0.0 23 1.94 0.14 32 54 58 405 15 17 39 2PF0 30 3O6A 33 2PC8 38 24 16 29 3N9K a BGC1 is the glucose residue from the nonreducing end of laminaripentose (L5) bound to the exterior of the protein b ... mutation-containing pFOX fragments into vector pGSB1, a pUC19 derivative containing the complete EXG ORF The entire ORFs containing each mutation were then cloned into the SnaBI site of pPIC9K After linearization...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
... membrane protein C precursor Outer membrane porin protein nmpC precursor Outer membrane protein F Glucosamine-fructose-6-phosphate aminotransferase 6707 Glucosamine-fructose-6-phosphate aminotransferase ... altered expression, and were thus selected for identification by peptide mass fingerprinting (PMF) Preparation of E coli protein extract for 2D-PAGE Protein identification by peptide mass fingerprinting ... variations in protein expression (11 protein spots) as compared with untreated cells The majority of these spots (Fig 12) were identified by peptide mass fingerprinting, reported in Table In particular,...
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Báo cáo khoa học: Autoregulatory binding sites in the zebrafish six3a promoter region define a new recognition sequence for Six3 proteins potx
... The following deletion constructs were made: pS3aPG, pS3aPGDa1,a2,t2 (or pS3aPGDa1), pS3aPGDa3–a5 (or pS3aPGDa3), pS3aPGDa6,a7,t 13 (or pS3aPGDa6), pS3aPGDa8–a11,t15 (or pS3aPGDt15), pS3aPGDa12,t17 ... pS3aPGDa12,t17 (or pS3aPGDa12), pS3aPGDa 13 a15 (or pS3aPGDa14), pS3aPGDa16–a18 (or pS3aPGDa18), pS3aPGDa1.2, and pS3aPGDat15.2 Following PCR amplification of the whole plasmid except the region to ... Δ424 bp + pS3aPGΔa14 + Δ470 bp pS3aPGΔt15 * + 36 8 bp pS3aPGΔa6 pS3aPGΔa12 + Δ406 bp + 38 6 bp pS3aPGΔa1 * Fig Deletion of particular fragments within the six3a promoter region affects reporter...
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Báo cáo Y học: Investigations into the mechanisms used by the C-terminal anchors of Escherichia coli penicillin-binding proteins 4, 5, 6 and 6b for membrane interaction ppt
... (1997) Membrane binding of Escherichia coli penicillin -binding protein is predominantly electrostatic in nature and occurs at a specific binding site Prot Peptide, Lett 5, 63 66 13 Jackson, M.E & Pratt, ... the membranebinding domain of penicillin -binding protein of Escherichia coli Mol Microbiol 2, 5 63 568 Phoenix, D.A & Pratt, J.M (19 93) Membrane interaction of Escherichia coli penicillin -binding ... B., Leppimaki, P & Slotte, J .P (2001) Cholesterol interactions with phospholipids in membranes Prog Lipid Res 41, 66–97 Mottl, H., Terpstra, P & Keck, W (1991) Penicillin -binding protein of Escherichia...
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Báo cáo khoa học: "Immunohistochemical localization of calcium binding proteins and some neurotransmitters in myenteric plexus of goat stomach" doc
... etairporppa eht htiw )aeroK ,ygolonhceTMi( aremac latigid 0002TMI na ot dehcatta )napaJ ,supmylO( epocsorcim 15XB supmylO na rednu devresbo erew snoitaraperp edils ehT lorecylg %08 gniniatnoc SBP ... ,84-12 pp de eussieR slamina yrotarobal fo esu dna erac eht rof ediuG licnuoC hcraeseR lanoitaN 11 631 -32 1 , 03 ,2991 lohproM J ruE senoruen ciretne gip-aeniug fo yrtsimehcotsihonummi editpep dna ... sneH 744- 134 ,8 23 ,2891 loisyhP J peehs dezimotogav yllacinorhc eht ni ytilitom hcamotseroF CP yrogerG 5111-6011 , 433 ,6991 deM J lgnE N metsys suovren ciretne ehT I onariH ,KR layoG 1 03- 392 ,652...
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Báo cáo y học: "Pharmacological disruption of insulin-like growth factor 1 binding to IGF-binding proteins restores anabolic responses in human osteoarthritic chondrocytes" ppt
... – 3. 3 3. 3 3. 3 3. 3 – 13. 3 13. 3 13. 3 13. 3 NBI -31 772 (µM) – – 0.1 10 – – 0.1 10 Effecthuman osteoarthritic chondrocytes incubated with IGF-1of insulin-like growth factor (IGF)-1 to IGF -binding proteins ... growth factor binding proteins using Western blotting: use of the method for titration of the binding proteins and competitive binding studies Anal Biochem 1986, 154: 138 -1 43 37 Bhakta NR, Minerva Garcia ... the binding of insulin-like growth factor (IGF) to IGF -binding proteins Bioorg Med Chem Lett 20 03, 13: 1 931 -1 934 50 Roelfsema V, Lane MH, Clark RG: Insulin-like growth factor binding protein (IGFBP)...
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