protocols of two dimensional difference gel electrophoresis to investigate mechanisms of toxicity
Báo cáo y học: "Two-dimensional difference gel electrophoresis (DIGE) analysis of sera from visceral leishmaniasis patients" ppt
... 15 mA per gel at 20°C for 30 minutes, followed by 30 mA per gel at 20°C until the bromophenol blue dye front ran off from the bottom of the gels Six such gels were run corresponding to six biological ... generated for each gel Eighteen images were generated in total corresponding to the six gels A representative DIGE gel showing the overlay of Cy3 and Cy5 images from one such gel is shown in Figure ... C1-inhibitor also has anti inflammatory property independent of its proteolytic activity [19] Hemolysis due to activation of alternate complement pathway is one of the major causes of anaemia...
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Báo cáo y học: "Proteome changes of lungs artificially infected with H-PRRSV and N-PRRSV by two-dimensional fluorescence difference gel electrophoresis" pps
... MALDI-TOF mass spectrometry and tandem TOF/TOF mass spectrometry analyses were performed on an AutoFlex TOF-TOF LIFT Mass Spectrometer (Bruker Daltonics) according to the manufacturer's instructions ... response to apoptotic stimuli All of these indicated that caspase cleavage of NDUFS1 is essential to several changes of mitochondrion during apoptosis[44] On the other hand, reduced expression of NDUFS1 ... http://www.virologyj.com/content/7/1/107 Page of 17 Table 3: Different expression of proteins between H-PRRSV and N-PRRSV (N96/H96, H168/H168) inoculated lungs identified by MALDI-TOF or MALDI-TOF/TOF Master no.a Accession...
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báo cáo khoa học: " Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis" pdf
... reference gel of the software-assisted analysis the values of pI predicted for any given length of the strip Both Mr and pI of the spots of interest were then determined by using software-automated ... The aim of this work was to apply a proteomic approach to study the changes in protein patterns of root and leaf organs of maize plants in the first phase of exposure to high availability of nitrate, ... content of total protein (mg g -1FW) and the % yield of the extraction of soluble proteins (% of extracted soluble proteins respect to the total content) for the roots and the leaves of the plants...
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Báo cáo khoa học: "Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites" ppt
... expression by two- Two- dimensional gel electrophoresis and its immunoblot analysis of Neospora caninum tachyzoites dimensional electrophoresis and mass spectrometry: proteomics of Toxoplasma gondii ... (KBA-2) mixed with Freunds complete (first Two- dimensional gel electrophoresis and its immunoblot analysis of Neospora caninum tachyzoites 141 Fig Comparison of two IEF conditions IEF was performed ... Immunodominant antigens of N caninum were detected as groups comprising molecules of 16/17, 29, 37, 46 kDa Two- dimensional gel electrophoresis and its immunoblot analysis of Neospora caninum tachyzoites...
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Báo cáo khoa học: "Contribution of two-dimensional electrophoresis of proteins to maritime pine genetics" ppt
... coding DNA) (Plomion et al, 1995b) Two- dimensional electrophoresis of denatured proteins (2D PAGE) allows the analysis of several hundreds of gene products in a single gel (O’Farrell, 1975) In gymnosperm ... visually by superimposition of the dried gels upon a light source Coelectrophoresis 1:1 of different tissues of the same genotypes were performed to ascertain the differences in spot position ... explained by the existence of major genes responsible for the determinism of polypeptide amounts A total of 38% of the proteins were clustered at two loci Each group of covariable spots could...
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Báo cáo khoa học: "Two-dimensional gel electrophoresis of membrane proteins from ectomycorrhizal fungi" doc
... awarded to FM and by a doctoral fellowship of the Institut National de la Recherche Agronomique and the Région de Lorraine to BH REFERENCES (1976) Two- dimensional gel electrophoresis of membrane ... incomplete solubilisation of the membrane sample leading to the formation of protein complexes and aggregates that remain at the top of the focusing gel or move slowly into the gel during focusing ... free of streaking, smearing and background staining, lack artifacts due to prote- olysis, and that protein patterns are reproducible from gel to gel The apparent resistance of membrane proteins to...
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Báo cáo hóa học: "Dynamics of a two-dimensional system of rational difference equations of Leslie–Gower type" doc
... β1 ⇒ λ1 < The eigenvector of T at E1 that corresponds to the eigenvalue l1 < is (1, 0) The rest of the proof is similar to the proof of part (ℛ6) (ℛ8, ℛ9) The first part of theorem follows from ... Asymptotic behavior of a linear fractional system of difference equations J Inequal Appl 127–143 (2005) Clark, D, Kulenović, MRS, Selgrade, JF: Global asymptotic behavior of a two dimensional difference ... 1.i) of Lemma 1, then the proof of Theorem is similar to the proof of parts (ℛ9) and (ℛ10) This completes the proof of Theorem in the regions ℛ12, ℛ13 This is an example of semistable non-hyperbolic...
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Báo cáo y học: "Psoriasin, one of several new proteins identified in nasal lavage fluid from allergic and non-allergic individuals using 2-dimensional gel electrophoresis and mass spectrometry" potx
... The controls were all symptom-free, had no history of allergic rhinitis and had negative SPT to the standard panel of allergens as described above They had no history of upper airway infection ... fluid Of the spots picked from the 2D-gels and submitted to MALDI-TOF analysis 61 spots were identified (figure and table 1) 21 of these were identified as separate proteins The majority of the ... pool of samples was used To be able to load as much as 600 µg on the preparative gels pooled samples were further concentrated using Page of (page number not for citation purposes) Respiratory...
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Báo cáo sinh học: " A heuristic two-dimensional presentation of microsatellite-based data applied to dogs and wolves" pot
... proven to give deeper insight into the population structure of dogs [22], we could show that the method of two- dimensional plotting of distance matrices including consensus 2D graph and 2D graph of ... remaining breeds into two clusters The composition of the two remaining clusters depended on the alternative clustering of PW, ES and GR breeds Therefore, the third cluster consisted of one to three breeds ... dyes We used M13MP18-tailing for primers to combine various four to six markers into multiplex sets labelled with one of three fluorescent dyes and to be able to exchange individual markers as necessary...
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Báo cáo y học: "Segment-orientated analysis of two-dimensional strain and strain rate as assessed by velocity vector imaging in patients with acute myocardial infarction"
... -11.67 ± 5.38 %; systolic SR (sSR) -0.65 ± 0.27 s-1; early diastolic SR (Sre) 0.60 ± 0.35 s-1 The analysis of tissue velocities demonstrated a gradient of systolic (S´) and diastolic (early E´ and ... cut-off value 15 for strain of –6.5 % a sensitivity of 23 % and specificity of 87 % was calculated for the detection of infarcted segments (AUC 0.6, confidence interval 0.53 – 0.64) A 111 cut-off ... wall thickness or no LE but LAD perfusion territory) and non-infarcted 107 (LE 0%, no LAD perfusion territory)9 The results of the VVI offline analysis of the tissue velocities (S´, E´, A´) derived...
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Tài liệu Huntington’s Borghese Style Urn as a Study of Two-Dimensional Art & The Production of a Piece of Two-Dimensional Art pptx
... Huntington & Scott Gallery Programs Relief Sculpture in Two Parts What does two- dimensional mean? What things are two- dimensional? Describe this sculpture Is it two- dimensional or three -dimensional? ... goal) The Huntington Library, Art Collections, and Botanical Gardens Huntington & Scott Gallery Programs Relief Sculpture in Two Parts PART II THE PRODUCTION OF A PIECE OF TWO- DIMENSIONAL ART ... available Tips: The top of a piece of drywall is the side that tapers down at the edges, this side usually has a lighter colored paper The bottom of a sheet of drywall tends to have the manufacturers...
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Báo cáo khoa học: "A Grammatical Approach to Understanding Textual Tables using Two-Dimensional SCFGs" docx
... for a dimensional model so on Tables were extracted from the collected sample, automatically cleaned, and tokenized into two- dimensional array of tokens 911 priate semantic interpretations of textual ... direction of parsing The two- dimensional grammar notation includes of a set of nonterminals, terminals, and two generation operators “–>” and “|->” Let X be a nonterminal and Y, Z, be two symbols ... construction of the two- dimensional grammar Each tokenized table was tagged by the human judge with a list of types T relevant to the table The relevance is defined as follows: a data model is relevant to...
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Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot
... structure of 282 residues of a total of 344 have been solved [19] The catalytic domain of PKCh possesses a high degree of homology ( 80%) with the 3280 catalytic domain of other isoforms of PKC ... reason it is of great interest to study the structure of the catalytic domain of PKCf and its interaction with substrates To date, no full structure of a complete PKC isoform, at an atomic resolution ... spectroscopy to study the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the presence and the absence of...
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Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx
... addition to the generation of Ab peptides in pathological processes Study of the characteristics of the PS complex will facilitate elaboration of the molecular processes involved in the production of ... before endoproteolysis to generate heterodimers of NTF and CTF components consistent with previous reports [47] Sensitivity of the PS complex to SDS and Triton X-100 and stability to reducing agent ... in vivo: suggestion of novel regulatory mechanisms leading to higher order complexes Biochem Biophys Res Commun 301, 119–126 50 Hu, Y & Fortini, M.E (2003) Different cofactor activities in gamma-secretase...
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Đề tài " (log t)2/3 law of the two dimensional asymmetric simple exclusion process " pdf
... proportional to |x|S(x, t) (1.1) x Therefore, Johansson’s result on the variance of the current can be interpreted as the spreading of S(x, t) being of order t2/3 The result of Johansson is for TWO DIMENSIONAL ... analysis of the Green function of the dynamics One first used the duality to map the resolvent equation into a system of infinitely-coupled equations The hard core condition was proved to be of lower ... conjugate To check the combinatorics, we notice that the total number of terms is n(n + 1) n(n + 1) (n − 1)(n − 2) , + 2(n − 1) + = 2 the same as the total number of terms in (AF )2 The factors are...
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Báo cáo khoa học: Analysis of the CK2-dependent phosphorylation of serine 13 in Cdc37 using a phospho-specific antibody and phospho-affinity gel electrophoresis doc
... phospho-affinity gel electrophoresis Analysis of Cdc37 phosphorylation and dephosphorylation by phospho-affinity gel electrophoresis To analyze the time course of Cdc37 phosphorylation in vitro, mixtures of ... factors, insulin, and epidermal growth factor cause rapid cytoskeletal reorganization in KB cells Clarification of the roles of type I insulin-like growth factor receptors and insulin receptors ... concentration of NaCl in the samples was adjusted to 265 mm before heat denaturation Electrophoresis was stopped when a prestained 40 kDa protein marker reached the front edge of the gels, to give...
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![Báo cáo khoa học: A dimer of the FeS cluster biosynthesis protein IscA from cyanobacteria binds a [2Fe2S] cluster between two protomers and transfers it to [2Fe2S] and [4Fe4S] apo proteins ppt](https://media.store123doc.com/images/document/14/rc/tl/small_tlm1396203606.jpg)
Báo cáo khoa học: A dimer of the FeS cluster biosynthesis protein IscA from cyanobacteria binds a [2Fe2S] cluster between two protomers and transfers it to [2Fe2S] and [4Fe4S] apo proteins ppt
... with sequence similarity to IscS, one to the C-terminal domain of IscU and two to IscA [16,17] Because of the lack of operon organization the assignment of other genes to FeS cluster biosynthesis ... required to clarify the role of residue C44 Replacement of C110 and C112 led to a complete loss of cluster-binding ability of IscA Since the Mossbauer data ¨ indicated an all-cysteine ligation of the ... and Fish [31] Gel electrophoresis and Western blotting SDS/PAGE was carried out according to Seidler [32] and the gels were stained with Coomassie brilliant blue Nondenaturing gel electrophoresis...
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photocatalysis of two-dimensional honeycomb-like zno nanowalls on
... preparation of two- dimensional honeycomb-like ZnO nanowalls/zeolite composite material includes the coating of ZnO seed layer on surface of zeolite and the growth of two- dimensional ZnO First of all, ... zeolite To illustrate the effect of the two- dimensional honeycomb-like ZnO nanowalls/zeolite, a series of composite photocatalysts were designed and synthesized The purpose of this study was to detailedly ... EDS of two- dimensional honeycomb-like ZnO nanowalls/zeolite, which shows correct stoichiometry of ZnO to Al2O3 and SiO2 in the nanocomposite structure The mass percentage of the element of Zn,...
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Báo cáo hóa học: "Research Article Determination of Three-Dimensional Left Ventricle Motion to Analyze Ventricular Dyssyncrony in SPECT Images" pot
... a thickness of pixel/slice (6.47 mm) The volume of transverse tomograms was reoriented, and sets of slices perpendicular to the long axis (short axis view) and of slices parallel to the long ... component The tones of blue represent counterclockwise rotation while the tones of red represent clockwise rotation Column (c) presents the images of the vertical component The tones of blue represent ... the top right of the figure Before CRT After CRT +0.3 Systole −0.3 +0.3 Diastole −0.3 Figure 6: Horizontal motion of a Group1 patient A slice from the midcavity is shown in systole and diastole,...
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