... lipids of plant and animal origin, have been traditionally called fatsand oils.1 The difference between oils andfats is that fats are solids at room temperatures.2 Lipids, Fats, and Oils 133 TABLE ... 5.2 MAJOR OILS ANDFATS All oils and fats, with their high carbon and hydrogen content, can be traced back to organic sources Oils andfats are also produced by plants, animals, and other organisms ... F.D., The Chemistry of Oils andFats Sources, Composition, Properties and Uses, CRC Press, Boca Raton, FL, p 7, 2004; FSA (Food Standards Agency), Fatsand oils, McCance and Widdowson’s the Composition...
... (5¢-CTTCCTCCGCTTCCATGA-3¢) and QaCgClp1 (5¢-CCATGAAGTCCGCGAATC-3¢); and QsCgClp2 (5¢-GCATAGCGATGTGGACGA-3¢) and QaCgClp2 (5¢-GAGGACCGAGACCGTGAA-3¢) The abbreviations ‘Qs’ and ‘Qa’ refer, respectively, to sense and ... primer, 2.5 mm MgCl2 and U of Taq DNA polymerase (Eurogentec, Liege, Belgium) The reaction was cycled between 94 °C, 50 °C and 72 °C (45 s, 60 s and 90 s, respectively), and this was followed ... edge and postspawning gonads Metamorphosis represents the ultimate stage of oyster development, and is characterized by the degeneration of larval tissues, such as the velum and the foot, and...
... identified messengers coding for proteins involved in cell proliferation (MIF and cenpA), survival (i.e survivin, BI-1, and dad1), and ribosome biogenesis (members of the small and large ribosomal subunits) ... availability of growth factors, hormones, and nutrients, result in the activation of signaling pathways that rapidly and reversibly modulate the translation of RP mRNAs and the other TOP mRNAs (Fig 1) ... mammalian RPs, 49 are related to archeal RPs, and 32 are homologous to bacterial proteins [8] The remaining 11 RPs are specific for eukaryotic ribosomes and may be involved in additional particular...
... behaviour of TAadm and TAdmdR1 strains regarding production of antibiotic suggests interaction between the actions of the Adm and DmdR1 proteins (see below) Lack of both DmdR1 and Adm proteins prevents ... formation of desferrioxamines B and E (labelled as DesB and DesE) and complementation of desferrioxamine production (A) Cultures of S coelicolor A3(2) and the TAdmdR1, TAadm and DdmdR1 ⁄ adm mutants ... genes and iron regulation A B C Fig Immunodetection of DmdR1 and Adm proteins (A,B) Western blot reactions of wild-type S coelicolor A3(2) (lane 2) and the TAadm (lane 3), TAdmdR1 (lane 4) and...
... also reports of dual-targeted proteins to chloroplasts and the nucleus [7], to chloroplasts and the peroxisome [8,9], and, in Chlamydomonas reinhardtii, to chloroplasts and the endoplasmic reticulum ... provided, and the insights that may be gained from a greater understanding of the mechanism of dual targeting for the targeting of location-specific proteins is discussed Targeting signals and mature proteins ... locationspecific proteins Why dual target proteins? Mitochondria and chloroplasts share many common enzymatic steps that are catalysed by location-specific proteins [44] Thus, it is unclear why some and, ...
... between a strand and helix; and (f) between the side chains Arg452 and Glu437 of strands FEBS Journal 272 (2005) 1819–1832 ª 2005 FEBS S Rajagopal and S Vishveshwara Short hydrogen bonds in proteins ... the formation of SHB in this figure and all the subsequent figures are shown in ‘ball and stick’ representation and the secondary structures (helices and strands) in ‘cartoon’ representation c ... of strands (Fig 13, a), strands moving away in different directions (Fig 13, b), SHBs in the beginning of the sheet (Fig 13, c), at the end of the sheet (Fig 13, d) and between a strand and helix...
... CSPs on transcription and translation Many CSPs have been shown to have a high afnity to single-stranded nucleic acids and therefore are thought to act on transcription and or translation For ... Y-box proteins that are known to bind DNA and RNA [4] It is a member of the protein family of bacterial CSPs who form a group of highly homologous 7.4 kDa proteins known to bind to RNA and ssDNA ... results in a prominent protein band on the SDS PAGE whereas the two wildtype sequences pET14bRasc and pET14bRas not result in a visible band on the Coomassie stained gel and can only be detected by...
... and MRPs Silymarin and quercetin were the best inhibitors for both MRP1- and MRP5-mediated efflux Naringenin, hesperetin, resveratrol and daidzein at 50 lm had moderate to no effect on MRP1- and ... exposed to [32P]8-azidoATP[aP] alone, lanes 2, and membranes treated with 10, 50 and 100 lM quercetin, respectively, and lanes 6, and 8, with 10, 50 and 100 lM silymarin, respectively Lane 9, crude ... MRP1, MRP4 and MRP5, and that some of them, i.e quercetin and silymarin, may well prove to be substrates for MRPs They modulate both the transport function and ATPase activities of MRP1 and MRP4...
... respectively, were: -0.54 and -0.25 for rice, -0.54 and -0.35 for wheat flour, -0.09 and -0.03 for coarse grains, -0.96 and -0.33 for pork, -0.03 and -0.40 for eggs, and -0.39 and -0.47 for edible ... on all fats, a tax on saturated fats, a tax on added sugar and subsidies on fibres For most consumers, a tax on fats (whether all fats or saturated fats) would reduce total energy intake and its ... participants were randomized into five groups including no treatment, standard behavioural treatment (SBT), SBT and food provision, SBT and incentives and SBT, food provision and incentives The...
... an open question if ESP and NSP share any biochemical properties and whether nitrile formation mediated by these two proteins occurs via the same mechanism Because ESP and NSP activities have ... myrosinase After a washing step, ESP and bound proteins were eluted Loading, washes and elution were monitored by western blot analysis with an anti-ESP serum (A) and by myrosinase assays (B) (L1, ... Burow et al Nitrile-forming proteins from plants and insects Table Nitrile and epithionitrile formation from different glucosinolates in the presence of myrosinase, ESP and NSP in vitro Assays were...
... membrane vesicles shown in Fig The maximum uptake rates, Umax1 and Umax2, the dissociation constants, Kd1 and Kd2, and the Hill coefficients, n1 and n2 are defined as indicated in Eqn (1) The data were ... erythrocyte membrane vesicles The maximum uptake rates, Umax1 and Umax2, the dissociation constants, Kd1 and Kd2, and the Hill coefficients, n1 and n2 are as defined in Eqn (1) The data were collected ... MRP family Immunodetection of MRP4 and MRP5 proteins in human erythrocytes and COR-L23/R cells Fig Effect of cGMP on the DNP-SG transport in human erythrocytes and vice versa (Top) ATP-dependent...
... [19] and Hedge & Das [20] were studying the proteins p36 and p38, respectively The proteins may be identical: both proteins are phosphorylated in response to the presence of Ras protein and guanine ... osmoregulation system in yeast The SLN1 and SSK1 proteins of Saccharomyces cerevisiae have sequence similarities to both the histidine kinase and the response regulator proteins from bacteria, respectively ... twocomponent proteins Two-component systems are present in both Gram positive and Gram negative bacteria In addition to housekeeping functions, they also control expression of toxins and other proteins...
... structure and function in proteins to select for structurally related proteins These experiments shed light on the sequence determinants of folding and the evolutionary pressures on protein folding and ... residue random proteins, Davidson & Sauer explored characteristics of a simplified library containing only glutamine (Q), leucine (L) and arginine (R) They estimated that > 5% of the proteins ... Escherichia coli and 1–2% showed cooperative unfolding (a characteristic of small folded proteins) and helical secondary structure, but lacked good tertiary packing [21,22] Few, if any, of the proteins...
... E coli HypC and HybG [14], in R eutropha HypC and HoxL [33,35], in R leguminosarum [36], R capsulatus and Bradyrhizobium japonicum [2] HypC and HupF, and in T roseopersicina HypC1 and HypC2 Our ... consists of 156 amino acids (16.6 kDa) and the transposon is inserted into Table Identity between the accessory proteins of T roseopersicina and the corresponding proteins from other organisms T roseopersicina ... the proteins appeared variable The highest homology was found at the C-terminus and, the hynD gene at bp 107 of the 471 bp-long gene Thus, the hypC2 and the hynD genes are separated by 120 bp, and...
... [1-3] Components of this system include the ligands IGF1 and IGF2, their cell surface tyrosine kinase receptors IGF1R and IGF2R, and seven IGF binding proteins (IGFBP) IGF1R has been shown to play ... mean and median IGFBP-3 and -4 tumor expression and sera concentrations, patients were grouped into those with primary disease and those with metastatic disease The relationship between IGFBP-3 and ... percentile and are indicated by points outside of the box and whiskers 39 patients had both sera and tumor specimens available for correlation of IGFBP-3 expression and 56 patients had both sera and...
... activity of RVFV Gn and Gc proteins Fusogenic activity of RVFV Gn and Gc proteins A) Insect cells were infected with a baculovirus expressing RVFV N, Gn and Gc for 24 hours and a monoclonal antibody ... purification of proteinsand VLPs, and EM studies CC carried out cell surface expression and cell to cell fusion studies PR contributed in the coordination and design of the study and helped in ... baculovirus expressing Gn, Gc and N proteinsand 30 hours post-infected cells were fixed and processed for immunofluorescence Since these cells were not permeabilized only proteins expressed in the...
... Bavari, and Jasenosky [4,5,13], and expand upon these findings by incorporation of NP and VP35 into VLPs Our data demonstrate that VLP density increased with the inclusion of the nucleocapsid proteins ... study EBOV proteins is transfection and co-expression of plasmids coding for individual viral proteins Using this approach, Bavari et al have demonstrated that co-expression of VP40 and GP yielded ... transferred and probed with anti-VP40 antibody Transfected cells were lysed in RIPA buffer and electrophoresed over a 10% SDS-PAGE gel, transferred and probed with anti-VP40, anti-GP, anti-NP, and anti-HA...
... sites for the Cbf1 and CBF3 proteins [49] The consensus DNA sequences and their binding proteins are recognizably similar in yeasts as distantly related as Candida glabrata and Kluyveromyces lactis, ... Agriculture, Bernd Weisshaar, and Ian Wilson for supplying cDNAs, and Andreas Houben for supplying wheat and barley genomic DNAs References K-estimator [31] was used to estimate Ka and Ks in comparisons ... Positive Negative Figure Comparisons of CENP-C proteins in animals, yeast and plants The CENPC motif and conserved regions found at the termini of CENP-C proteins are indicated For pairwise comparisons...
... to prevent and to stabilize complexes of proteins with macromolecules and with small molecules A correlation has been found between the pH optimum of stability of monomeric proteinsand of their ... based on the known number and calculated pKa of weak acids and bases, and on emerging data of protein localization and concen trations There is also need for improved understanding of the diffusion ... of thermophilic proteinsProteins from thermophilic organisms usually have a higher number of charges and ion pairs than their mesophilic homologs [7] An analysis of the number and types of charges...
... fibrous matrix and osteoblasts BMP3 was found in osteoblasts and osteoclasts, BMP6 in osteocytes and osteoclasts, and BMP7 in hypertrophic chondrocytes, osteoblasts and osteocytes Nakase and colleagues ... cells and immune cells are likely to be critical in this process and may contribute to the differences between destructive and remodeling arthritis In this context it is noteworthy that we and ... Flanders (FWO-Vlaanderen), a grant from the KU Leuven (GOA) and a EULAR Young Investigator Award to Rik Lories Rik Lories is a post-doctoral fellow from the Fund for Scientific Research Flanders...