prostacyclin thromboxanes and intracellular granules
Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf
... cells and their passive uptake [10,31] and (b) competition between the active pumping of the agents out of the cells and their intracellular binding to receptors and ⁄ or pumping into intracellular ... [15–18] On the other hand, MRP1 is present in virtually all human tissues and in most human tumor cell lines and tumor samples [4] Using mice genetically deficient in both the mdr1a and mdr1b genes ... cells remain free in the cytoplasm and are passively bound to various intracellular sites Likely sites are the intracellular membranes because they are hydrophobic and negatively charged A comparison...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... four-band pattern at SDS/PAGE (Fig 1) At comparison with insect cell produced recombinant cystatin F (Fig 1, lanes and 2), which appears as one mono- (band B) and one di-glycosylated (band A) ... allowed to sink and the supernatant was discarded Sample buffer containing SDS and reducing agent was added and proteins were separated electrophoretically on 16% SDSpolyacrylamide gels and the buffer ... were rinsed in · NaCl/ Cit and washed in · NaCl/Cit and 0.1% SDS for 45 at room temperature; 0.2 · NaCl/Cit and 0.5% SDS for 45 at room temperature; 0.2 · NaCl/Cit and 0.5% SDS for 30 at 50 °C...
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Báo cáo sinh học: " Genome degeneration affects both extracellular and intracellular bacterial endosymbionts" pot
... Nutrition Location Intracellular in bacteriocyte Intracellular in bacteriocyte* Intracellular in bacteriocyte Cell associated Intracellular in bacteriocyte Intracellular in bacteriocyte* Intracellular ... regulators, and recombination and DNA repair factors, and high mutation rates [6-8] Similar genomic features are also observed in pathogens, including Mycoplasma species and obligate intracellular ... intra- and extracellular endosymbionts, future genome analysis of Ishikawaella and Rosenkranzia will reveal whether there are basic differences in the gene pools retained between extraand intracellular...
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Báo cáo y học: "Expression and intracellular localization of duck enteritis virus pUL38 protein" pptx
... JX, GPM and SCZ carried out most of the experiments and drafted the manuscript ACC, MSW, DKZ, RYJ, QHL, ZLC and XYC helped in experiments and drafted the manuscript All authors read and approved ... kinase pUS3 and dUTPase, were first detected at h.p.i and h p.i respectively [9,10] In contrast, identified late products– tegument protein pUL31 and pUL51–were first detected at h.p.i and h.p.i., ... with DEV at an MOI of The cells were processed at h, 18 h, 30 h, and 56 h.p.i., and pUL38 was detected using pUL38-specific antibody and fluorescein isothiocyanate (FITC)-conjugated secondary antibody...
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Báo cáo y học: " Elemental analysis of lung tissue particles and intracellular iron content of alveolar macrophages in pulmonary alveolar proteinosis" ppt
... the distribution of S The intracellular content and distribution of Fe, S and P in an alveolar macrophage are shown in mixed colors (Mix) as follows: Fe (red), S (green), and P (blue) A serial section ... Si, and S in lung tissue is shown by gray to white dots Cell morphology was identified by the distribution of P located in normal lung area The intracellular content and distribution of Fe, S and ... in particles and alveolar macrophages, and for determining the intracellular iron content in alveolar macrophages Secondary PAP is associated with exposure to inhaled particles and accumulation...
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The study of interactions of transmembrane receptors and intracellular signaling proteins in live cells by fluorescence correlation and cross correlation spectroscopy
... to investigate the intracellular interactions between Cdc42 and its effectors The concentrations of bound complexes ( cGR ) and unbound EGFP- and mRFP-fusion proteins ( cG and c R ) were determined ... length of optical lens dimer fraction ρ β f R2 ω and z f G and f R η or cps λ μs μW 2D and 3D coefficient of determination ω and z are the radial and axial distances of the focal volume at which ... ligands and receptors (31, 131-133), enzyme kinetics (134), and DNA reactions (135) For example, Pramanik and Rigler used Rh-EGF to plot the association/dissociation constants between EGF and...
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Tài liệu Intracellular Traffic and Neurodegenerative Disorders pptx
... Lindquist and Karen L Allendoerfer Tau and Intracellular Transport in Neurons 59 E.-M Mandelkow, E Thies, S Konzack, and E Mandelkow Signaling Between Synapse and Nucleus ... Intracellular Traffic and Neurodegenerative Disorders RESEARCH AND PERSPECTIVES IN ALZHEIMER’S DISEASE Peter H St George-Hyslop Yves Christen • William C Mobley Editors Intracellular Traffic and ... generation; and (4) sorting of APP and/ or its α- and β-carboxyl-terminal fragments (C83 and C99, respectively) toward the trans-Golgi network is under the influence of presenilins and the VPS35/retromer...
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Tài liệu Báo cáo khoa học: PC1⁄3, PC2 and PC5⁄6A are targeted to dense core secretory granules by a common mechanism doc
... secretory granules by PC enzymes such as PC1 ⁄ 3, PC2 or PC5 ⁄ 6A Understanding how these enzymes and their substrates are targeted to secretory granules is thus critical in understanding this ... with [35S]methionine for h, and chased for h in complete medium The chase supernatant was simultaneously immunoprecipitated for the fusion protein and endogenous PC1 ⁄ 3, and the precipitated proteins ... endogenous ACTH (present primarily in dense core secretory granules) and its precursor POMC (primarily present in the endoplasmic reticulum and Golgi apparatus) Note the relative staining distribution...
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Tài liệu Báo cáo khoa học: Platelet factor 4 disrupts the intracellular signalling cascade induced by vascular endothelial growth factor by both KDR dependent and independent mechanisms ppt
... VEGF-induced MAP kinase activation and cell proliferation [29] To distinguish between the extracellular effects of PF-4 acting on ligand/receptor activation and intracellular effects on signalling ... obtained ` through local maternity units (Lariboisiere Hospital and Saint Isabelle Clinic) under approval, and with appropriate understanding and consent of the subjects DNA synthesis HUVEC were seeded ... induced by VEGF165 and VEGF121 was maximal between 10 and 15 of stimulation and decreased thereafter (Fig 4C,E) PF-4 strongly decreased ERK phosphorylation, to only 34% (VEGF165) and 22% (VEGF121)...
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Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf
... in the T-cell lines Jurkat (Fig 9A) and D23II-7 (Fig 9B) both TNF and PMA/Ionomycin, induced a significant and reproducible up-regulation of TRAF4 mRNA TNF- and PMA-induced TRAF4 expression was ... Localization and transcriptional regulation of TRAF4 (Eur J Biochem 269) 4827 Fig TNF and PMA up-regulate TRAF4 mRNA in T-cells Jurkat (A) and D23II-7 T-cells (B) as well as primary human T-cells and ... mRNA signal To obtain relative TRAF4 and L32 expression values the ratio between the signal intensities of bands of treated cells and the corresponding band of the untreated group were calculated...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... anti-HA and anti-BiP Ig, and processed for fluorescence microscopy Co-localization masks were created as described in Materials and methods, and represent areas with overlapping green and red ... class I molecules and the protein is organized into a1, a2 and a3 structural domains that resemble those described for MHC class I and related proteins [4,16] The N-terminal a1 and a2 domains come ... and methods Cell culture HEK293T and HuTu80 cells were obtained from the American Type Culture Collection and cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum and...
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Báo cáo khoa học: Induction of PPARb and prostacyclin (PGI2) synthesis by Raf signaling: failure of PGI2 to activate PPARb potx
... Cox-2 and ERK-dependent increase in the synthesis and release of PGE2 and PGI2 which was mainly due to a strong transcriptional activation of the Cox-2 gene (and to a lesser extent of PGIS and ... fatty acids and the synthetic agonists carbaprostacyclin (cPGI) and GW01516 For experimental details see legend to Fig Values represent the average of triplicates; error bars show the standard deviation ... agonists carbaprostacyclin (cPGI) and GW501516, it was consistently and reproducibly seen Treatment with AA resulted in an approximately sixfold increase in 6-k-PGF1a in the culture medium, and this...
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Báo cáo khoa học: The ROQUIN family of proteins localizes to stress granules via the ROQ domain and binds target mRNAs pdf
... novel and highly conserved ROQ domain mediates induction and recruitment to stress granules HsROQUIN, HsMNAB, RLE-1 and DmRoquin are highly conserved in the N-terminus and all localize to stress granules, ... localizes to stress granules and binds RNA in the ability to induce stress granules and importantly was not recruited to TIA-positive stress granules even in stressed cells (Figs and S3D) By contrast, ... localizes to the nucleus and cytoplasm and, upon environmental stress, concentrates in stress granules The novel ROQ domain mediates localization to stress granules and induces spontaneous stress...
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Báo cáo khoa học: Lysosomal localization of GLUT8 in the testis – the EXXXLL motif of GLUT8 is sufficient for its intracellular sorting via AP1- and AP2-mediated interaction docx
... sorting, and (c) understand the role of the EXXXLL motif in GLUT8 sorting The data provide evidence that endogenous GLUT8 co-localizes with the lysosomal proteins LAMP1 and LAMP2 in spermatocytes and ... completely abolish AP1 ⁄ and AP2 ⁄ GLUT8 NICD interactions (Fig 2C,D) This residual association with AP1 and AP2 might be due to high and variable concentrations of AP1 and AP2 in these extracts ... its intracellular retention and represents a strong intracellular sorting signal Our data are supported by a recent study that compared the [D ⁄ E]XXXL[L ⁄ I] sorting motifs between GLUT8 and...
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Báo cáo khoa học: Cytosolic phospholipase A2-a and cyclooxygenase-2 localize to intracellular membranes of EA.hy.926 endothelial cells that are distinct from the endoplasmic reticulum and the Golgi apparatus pdf
... downstream of cPLA2-a and convert arachidonic acid into prostaglandin H2 [8,9] To date, two distinct COX isoforms, COX-1 and COX-2, have been identified and characterized, and an alternative splice ... against COX-1 and COX-2 proteins Both COX isoforms have been shown to be constitutively localized on both the inner and outer surfaces of the nuclear membrane [14], and recently, COX-1 and prostacyclin ... cPLA2-a expression levels and subcellular distribution in EA.hy.926, HUVEC, A549 and HeLa cells (A) Total cell lysates from EA.hy.926, HUVEC, A549 and HeLa cells were prepared and 20 lg protein of...
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Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis pptx
... structural and sequence homology with EcCM, the active site residues of 90-MtCM can be identified, and are shown in Fig The striking difference between EcCM and 90MtCM is that the EcCM residues Ser84 and ... none of these residues is a candidate for performing the role of Gln88 in EcCM Of the other two structures, PfCM has the conserved Ser70 and Gln74, and TtCM has Ser81 and Glu85 There are two Protein ... (*EhCM) [16], *MtCM [5-7], and *YpCM; and AroH class CMs such as Bacillus subtilis (BsCM) [18]’ was corrected to ‘Erwinia herbicola CM (*EhCM) [16], *MtCM [5-7], and *YpCM; and AroH class CMs such...
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Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution by Spaska Angelova Stanilova pot
... levels of intracellular and soluble IL-1RA [82] Thus, an imbalance between IL-1 and IL-1RA may play an important role in the pathogenesis of GO and gene polymorphisms in IL-1α, -1β and/ or IL-1RA ... R, Greenberg, D A, & Davies, T F Common and unique susceptibility loci in Graves and Hashimoto dis‐ 25 26 Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution eases: results ... use, distribution, and reproduction in any medium, provided the original work is properly cited 34 Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution and the subsequent...
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Báo cáo khoa học: Spectroscopic characterization of the oxyferrous complex of prostacyclin synthase in solution and in trapped sol–gel matrix doc
... accompanied by a decrease in intensity, whereas the Q band (a collective term for the a and b bands), with an a-band peak at 570 nm and a b-band peak at 537 nm, was replaced with a broad peak at ... re-oxidized PGIS and sealed the cuvette with parafilm Again, the Soret peak was gradually red-shifted and after h of incubation it reached 422 nm, whereas the a bands and b bands were 557 and 528 nm, ... ligands We chose U46619 (an O-based ligand; a substrate analog whose oxygen atom at the C9 position is replaced with a carbon atom), NaCN (a C-based ligand) and clotrimazole (an N-based ligand)...
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Báo cáo khoa học: Characterization of a hemocyte intracellular fatty acid-binding protein from crayfish (Pacifastacus leniusculus) and shrimp (Penaeus monodon) pdf
... similarity in the ligand-binding pocket to bovine and human CRABP [5] However, recent ligand-binding studies with recombinant msCRABP showed high affinity binding for fatty acids and negligible interaction ... Vertebrate FABPs are involved in cellular fatty acid transport and utilization and compartmentalization of intracellular fatty acid storage, and also in fatty acidinduced regulation of gene expression ... Crayfish intracellular fatty acid-binding protein I Soderhall et al ¨ ¨ A B Fig Nucleotide sequence and deduced amino-acid sequence of plFABP and pmFABP (A) P leniusculus FABP cDNA nucleotide and...
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Báo cáo khoa học: Enhancement of intracellular concentration and biological activity of PNA after conjugation with a cell-penetrating synthetic model peptide docx
... 0.5 p.s.i and the separations were performed at 650 VÆcm)1 and 25 °C The peaks of the references appeared after 1.8 (I), 2.1 (e-fluoresceinyl lysine) and 4.2 (II) Apart from free I and II, the ... FACS and CLSM by providing absolute quantitation of internalized PNA CLSM revealed extensive fluorescence in the cytosol and nucleus of CM and CHO cells after exposure to both the naked PNA I and ... (trypan blue) and emission was measured at 515 nm and 570 nm, respectively Three regions of interest (16 · 16 pixel; 30 scans with a scan time of s with double averaging) in the cytosol and one in...
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