prospects for high throughput structure determination of proteins by nmr spectroscopy
DEVELOPING SOFTWARE TOOLS FOR STRUCTURE DETERMINATION OF LARGE PROTEINS BY NMR SPECTROSCOPY
Ngày tải lên :
04/10/2015, 15:45
... DEVELOPING SOFTWARE TOOLS FOR STRUCTURE DETERMINATION OF LARGE PROTEINS BY NMR SPECTROSCOPY ZHANG LEI B.SC (HONS.), NUS A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE GRADUATE ... cycle of refinement may go on several times before a high resolution structure can be determined Figure 1.5: Outline of the procedure for protein structure determination by NMR For the context of ... representation of pulse sequences used in multidimensional NMR experiments Figure 1.5: Outline of the procedure for protein structure determination by NMR 15 Figure 1.6: Effects of protein size on NMR signals...
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Structural and equilibrium unfolding studies of sam domain of DLC1 by NMR spectroscopy
Ngày tải lên :
14/09/2015, 14:04
... interaction 1.1.3 Structures of SAM domains 1.2 Protein structure determination by NMR spectroscopy 1.2.1 Fundamentals of NMR spectroscopy 1.2.1.1 NMR phenomenon 1.2.1.2 Basic NMR parameters 1.2.1.2.1 ... UNFOLDING STUDIES OF SAM DOMAIN OF DLC1 BY NMR SPECTROSCOPY THESIS BY YANG SHUAI SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMISTRY NATIONAL UNIVERSITY OF SINGAPORE 2009 ... method for high resolution structure determination 1.2.3 General strategy of NMR structure determination Protein NMR spectroscopy has become a very important technique in determining high- resolution...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
Ngày tải lên :
07/03/2014, 12:20
... particular promise for accelerated studies of proteins by NMR spectroscopy by assigning residue type information to every amide cross-peak observed in [15N]-HSQC spectra We anticipate that high yield ... the synthesis of proteins in the presence of other proteins provided in excess at the start of or during the reaction, e.g., for the purpose of rescuing nascently produced insoluble proteins into ... Dixon NE & Otting G (2004) Optimization of Escherichia coli system for cell-free synthesis of selectively 15 N-labelled proteins for rapid analysis by NMR spectroscopy Eur J Biochem 271, 4084–4093...
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Development and application of advanced proteomic techniques for high throughput identification of proteins
Ngày tải lên :
16/09/2015, 08:30
... AND APPLICATION OF ADVANCED PROTEOMIC TECHNIQUES FOR HIGHTHROUGHPUT IDENTIFICATION OF PROTEINS HU YI (B.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES ... aid of DNA sequence information, the elucidation of cellular functions of proteins is facilitated by large-scale protein profiling, i.e proteomics The significance of proteomics will be highlighted ... different aspects for high- throughput identification of enzymes and their associated proteins in yeast proteome (catalomics) Firstly, to validate the high- throughput capacity of differential gel...
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Báo cáo sinh học: "Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" doc
Ngày tải lên :
18/06/2014, 18:20
... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... class of systems described by (1) and ask which of those states are stable equilibria For this purpose denote an arbitrary positive steady state of the system by X ∗ , i.e X ∗ is a placeholder for ... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer...
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Báo cáo hóa học: " Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" pdf
Ngày tải lên :
20/06/2014, 01:20
... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... class of systems described by (1) and ask which of those states are stable equilibria For this purpose denote an arbitrary positive steady state of the system by X ∗ , i.e X ∗ is a placeholder for ... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer...
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Báo cáo toán học: " Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" ppt
Ngày tải lên :
20/06/2014, 21:20
... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... class of systems described by (1) and ask which of those states are stable equilibria For this purpose denote an arbitrary positive steady state of the system by X ∗ , i.e X ∗ is a placeholder for ... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer...
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Báo cáo y học: "ISsaga is an ensemble of web-based methods for high throughput identification and semiautomatic annotation of insertion sequences in prokaryotic genomes" pdf
Ngày tải lên :
09/08/2014, 22:24
... transpositionrelated (for example, regulatory) gene models (provided by ISfinder) for ‘.fasta’ input files The recommended genome input file for ISsaga is the GenBank format because this file format normally ... approaches with the advent of metagenomics ISsaga can contribute fundamentally to such studies in two ways: firstly by enriching the ISfinder database by high throughput annotation of completely assembled ... For example, for an IS composed of two ORFs, ISsaga will extract the nucleotide sequence starting from the coordinates of the beginning of the first ORF to the coordinates of the end of the second...
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Báo cáo y học: "Establishment of a novel CCR5 and CXCR4 expressing line which is highly sensitive to HIV and suitable for high-throughput evaluation of CCR5 and CXCR4 antagonists" pdf
Ngày tải lên :
13/08/2014, 13:20
... verified by viral replication assays in U87.CD4.CXCR4 and U87.CD4.CCR5 cells Therefore, we hypothesize that this partial blocking of R5 viruses by AMD3100 and of X4 virus by SCH-C is caused by disturbance ... after infection of cells The coreceptor use of each isolate is shown between brackets The data of one representative experiment out of two are presented Page of 13 (page number not for citation ... vitro models is a prerequisite for the successful setup of an accurate screening program for chemokine receptor antagonists Here, we Page of 13 (page number not for citation purposes) Retrovirology...
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Tài liệu Topical Fluoride Recommendations for High-Risk Children Development of Decision Support Matrix pptx
Ngày tải lên :
12/02/2014, 19:20
... fluoride use for high- risk children This matrix was developed primarily for a nondental audience—programs, paraprofessionals, and professionals without formal dental education working with higher-risk ... consensus on several key areas for the purpose of informing the content of the decision support matrix (agenda in Appendix C) Discussions addressed the definition of high risk, which children meet ... prevention with the risk of fluorosis for high- risk children? n What are the areas of agreement among the existing professional guidelines? n How we stratify these guidelines by age group? Prior to...
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Báo cáo khoa học: ˚ cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosaminebinding hemagglutinin from Parkia platycephala seeds potx
Ngày tải lên :
16/03/2014, 13:20
... buffer A for min, followed by gradients of 0–30% of solution B (66.6% acetonitrile in A) for min, 30–40% B for 30 min, 40–70% B for min, 70–80% B for 10 min, 80–100% B for min, and 100% B for 10 ... and molecular mass determination of PPL2 (A) MALDI-TOF mass determination of native PPL2 purified by affinity chromatography as illustrated in the insert Insert: the fraction of a Parkia platycephala ... discarded and the lower chloroform phase was extracted four times with mL of distilled water The chloroform phases were freed of water by filtration through small columns made of a Pasteur pipette filled...
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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
Ngày tải lên :
16/03/2014, 18:20
... Escherichia coli by in-cell NMR spectroscopy J Am Chem Soc 123, 8895–8901 Gronenborn, A.M & Clore, G.M (1996) Rapid screening for structural integrity of expressed proteins by heteronuclear NMR spectroscopy ... applicability of the system with regard to protein analysis by NMR spectroscopy The catalogue of 15 N-HSQC spectra in Fig provides the basis for the straightforward identification of metabolite ... Cell-free protein synthesis enhanced by T7 RNA polymerase Prior to the preparation of 15N-labelled samples of hCypA and analysis by NMR spectroscopy, the performance of the cell-free expression system...
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laser bending for high precision curvature adjustment of microcantilevers
Ngày tải lên :
06/05/2014, 08:55
... developed a highly sensitive laser bending technique for adjusting the curvature of microcantilevers Such a technique is simple to implement, yet very useful for applications involving arrays of cantilevers ... cantilevers for parallel chemical and biological sensing The authors would like to thank Amit Gupta and Professor Rashid Bashir of Purdue University for supplying the microcantilever samples Support for ... field is about 10 m wide A total of eight lines can be applied to the cantilever of 110 m long, causing a maximum bending angle of 112 rad using laser energy of 0.3 J A finite element model...
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![bioinformatics for high throughput sequencing [electronic resource]](https://media.store123doc.com/images/document/14/y/gu/medium_Yb8L87hXir.jpg)
bioinformatics for high throughput sequencing [electronic resource]
Ngày tải lên :
29/05/2014, 14:33
... followed by the release of the Solexa Genome Analyzer by Illumina in 2006, which used sequencing by synthesis to generate tens of millions of 32 bp reads, and of the SOLiD and Heliscope platforms by ... steps of a high throughput sequencing project The “newbies” eager to learn the basics of high throughput sequencing technologies and data analysis will find what they yearn for specially by reading ... Bioinformatics for High Throughput Sequencing Naiara Rodríguez-Ezpeleta Ana M Aransay ● Michael Hackenberg Editors Bioinformatics for High Throughput Sequencing Editors...
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Báo cáo y học: "A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language" ppt
Ngày tải lên :
09/08/2014, 22:23
... accuracy Therefore, we always set the threshold for signal-to-noise ratios of peaks at the value of 3.0, which is a commonly accepted estimate for calculating the limit of detection (LOD) of analytical ... time -of- flight mass spectrometer [16] The interpretation of the MPIS dataset by LipidProfiler software confirmed 36 species representing 95% of the species identified manually The intersection of ... interepretation of a shotgun dataset by LipidXplorer takes advantage of independent use of several signature ions for each lipid class If detected at the high Table Multifaceted identification of bovine...
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báo cáo khoa học: " HAPIscreen, a method for high-throughput aptamer identification" pptx
Ngày tải lên :
11/08/2014, 00:23
... candidates Page of 10 Given the increasing demand for aptamers [13] it would therefore be more efficient to screen directly for the desired property i.e affinity of the target for a candidate ... demonstrate the interest of HAPIscreen (High throughput APtamer Identification screen), an AlphaScreen ® -based method for the detection of aptamers targeted to different RNA hairpins of eukaryotic or ... molecules of interest respectively immobilized on the two beads [20] (Figure 2A) Therefore monitoring the emission signal allows for the detection of complex formation We used this technology for monitoring...
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Báo cáo y học: "A method for high-throughput gene expression signature analysis" ppt
Ngày tải lên :
14/08/2014, 16:21
... were highly correlated, with 97.9% of data points falling within twofold of their corresponding means (Figure 2) Much of the variability was explained by a single transcript, accounting for 34% of ... the plate covered, spun as before, and incubated at 45°C for one hour followed by 65°C for 10 minutes Wells were emptied by centrifugation, as before A volume of 15 μl of a HotStarTaq DNA Polymerase ... analysis of the same material Plots depict log ratios of expression levels (tretinoin/DMSO) reported by both platforms for each transcript, in each of nine classes Correlation coefficients of the...
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BS EN 205 2003 adhesives wood adhesives for non structural applications determination of tensile shear strength of lap joints
Ngày tải lên :
30/08/2014, 11:14
... prepared by Technical Committee CEN/TC 193, "Adhesives", the secretariat of which is held by AENOR This European Standard shall be given the status of a national standard, either by publication of ... treatment of the test pieces before and after bonding; EN 205:2003 (E) Scope This European Standard describes tests for adhesives for wood and derived timber products for the assessment of their ... thickness of bond-line b = 20,0 ± 0,2 : width of test piece l1 = 150 ± 5: length of test piece s = 5,0 ± 0,1 : thickness of the panels c = grooves for thick bond-line Figure — Example of a bonded...
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