... characteristics ofthe "Sassi of Matera" and "Trulli of Alberobello" In the South of Italy, the sites ofthe "Sassi of Matera" (Figure 1a) (classified as humanity world heritage in 1993) and "Trulli of Alberobello" ... if the present structures are characterized with local materials, namely, “Tufo of Matera” for the structures ofthe "Sassi of Matera," and “Stone of Fasano” for the structures ofthe "Trulli of ... and the knowledge ofthe temperature coefficient ofthe material of which it is made A single measure allows for the determination of diffusivity, thermal conductivity, and specific heat of the...
... the SH sub-domain (item 1: ‘‘How often does your child have a lot of fun?’’ and item 4: ‘‘How often does your child feel happy?’’) The items ofthe SPS sub-domain Psychometric propertiesofthe ... smaller The RA and the PA sub-domains had lower correlations with their domains than most ofthe other domains at baseline, at endpoint, and Psychometric propertiesofthe CHIP-CE also for the change ... use the sub-domains rather than the domains ofthe CHIPCE when evaluating ADHD patients This is supported by the factor analysis based on the sub-domains and the correlation analysis ofthe sub-domains,...
... by presenting the pulse of Src activity in the plane ofthe inactive and active fractions, si and s1 If the duration ofthe stimulus exceeds the critical value, the trajectory in the (si, s1) ... state (in the bistability domain) Regardless ofthe slope, the active Src fractions increase during the Off to On transition, whereas the value ofthe inactive fraction (si) decreases if the slope ... all steady states ofthe Src cycle When there are three intersections, the steady state O1 at the lower branch ofthe Z-shaped curve (i.e low Src activity) and the state O3 at the upper branch...
... containing the structural zinc at the mouth ofthe cleft and thus exposed to the solvent [8] Gradual depletion ofthe enzyme first ofthe structural and then ofthe catalytic zinc has also been ... rate under the conditions present in the cytoplasm of Picrophilus The kinetics of NADPH degradation was followed by measuring the rate of decrease of its absorbance at 340 nm over the pH range ... abolished by the chelating agent EDTA However, the addition of Zn2+ did not affect the specific activity ofthe enzyme, and even high concentrations of EDTA (20 mm) could not decrease the activity of GdhA...
... cooperativity ofthe other redox centers In order to discriminate the contributions ofthe cofactors, inhibitors uncoupling or changing the cooperativity can be used Addition of cyanide strongly shifts the ... spectra and the deconvolution ofthe amide-I region A particular problem ofthe assignment in the difference spectra is the superposition of signals from different constituents ofthe oxidase, ... modes on the basis ofthe data presented here, in spite ofthe fact that bands in the difference spectra are observed in the region where the modes were attributed The vibrational modes of bound...
... below) The comparison of CD spectra of complexes also points toward slight differences in the conformation ofthe chelate rings The characteristic alternate pattern ofthe dd bands in the CD ... complexes of GGH, where the a carbon ofthe His residue is the sole source of chirality [10] However, while positions ofthe component dd bands and of CT transitions are relatively constant, their ... via the limitation of conformational freedom ofthe complex moiety The CD spectra of HSA complexes are intermediate between those of DAHam and DAHKam, suggesting that the conformation of the...
... as the pressure ofthe left upper corner ofthe loop; the significance of this pressure will be discussed in detail below Moving to the bottom ofthe loop, we can reason that the pressure ofthe ... instantaneous atrial volume) The pressure ofthe point at the bottom right corner ofthe loop is the pressure in the ventricle at the end ofthe cardiac cycle and is called the end-diastolic pressure ... mechanical properties by plotting the time course of change in the slope ofthe instantaneous PVR Above, we referred to the slope ofthe ESPVR as an elastance Similarly, we can refer to the slopes of the...
... b sheet with the b12 ofthe other monomer, and towards the C-terminus, the subsequent residues, Phe182Lys186, pass through the a2 vicinity ofthe other monomer For the FeR dimer, the N-terminal ... Resolution ofthe structure of NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side ofthe isoalloxazine ring, and that the 2Â-P-AMP moiety is held in the groove between the ... in the binding ofthe phosphate group(s) of FMN or FAD Lys92 forms a salt bridge with the O3P of FMN A salt bridge that involves avin species has not been reported in the structures ofthe other...
... domain of PABPN1 consisting of amino acids 1–125 ofthe wild-type protein (N-WT) Because the aim of this study was to investigate the effect on the fibrillation propertiesofthe most extreme disease-associated ... using the nanoscope analysis software Chemical stability of fibrils The chemical stability of fibrils was tested after the fibrillation process was completed (no further rise of ANS signals) The sample ... [26] The formation of amorphous aggregates was independent ofthe presence or length ofthe poly l-alanine sequence In contrast, no amorphous aggregates were observed with the N-terminal domain of...
... the total number of charges measured by electrospray MS Theoretical mass is the mass calculated on the basis ofthe protein amino-acid sequence The relative abundance refers to the ratio ofthe ... acetylation at one ofthe three residues, because the signal ofthe triacetylated species was less intense than that ofthe diacetylated species The MS/MS Ó FEBS 2003 Structural propertiesof SV-IV (Eur ... the mass increase of 42 mass units The relative level of acetylation of a peptide was estimated on the basis ofthe intensity ratio ofthe native and acetylated species From the data summarized...
... way to the wild type [16,17] The pKa for the alkaline transition being similar to that ofthe wild type, % 11.1 [18] Finally, comparison ofthe X-ray structures ofthe oxidized forms ofthe native ... disposition ofthe proximal His and Asp residues, affecting the imidazolate character ofthe His, but also by the formation of a LS species The latter indicating the probable binding of His42 to the ... impact on thepropertiesof His170 The strength ofthe hydrogen bond between the proximal His and Asp residues, and thus the imidazolate character ofthe His is expected to modulate not only the strength...
... amount at the 105–110 kDa size The amount of 105–110 kDa protein was reduced and in many instances evidence of aggregation was evident at the top ofthe gels The amount of aggregate present at the ... b-cyclodextrin-treated cells ofthe Na+/H+ exchanger, biochemical characterization ofthepropertiesofthe full length protein has proven difficult because of its low abundance and because ofthe difficulty ... that could either alter the distribution ofthe Na+/H+ exchanger or the constituency ofthe lipid rafts, we used an established detergent-free lipid raft isolation procedure for some of our studies...
... Cc of Asp and Cd of Glu, and Og of Tyr Positive unit charges were added at the following atoms: Nt ofthe N-terminus, Nf of Lys, Cf of Arg, and Nd or Ne of His (depending on the position ofthe ... shows the van der Waals model ofthe top face ofthe best structure of RIIa D/D in the region )1 to 44 This view is generated by rotating the orientation of RIIa D/D of Fig 4A by 90° about the ... hydrophobic interface ofthe two monomers (Fig 6B) A further rotation ofthe orientation of RIIa D/D of Fig 4B by 180° about the x-axis shows the bottom face of RIIa D/D (Fig 4C) There is some hydrophobic...
... conserved in the primary structure ofthe GTPase domain of hGBP5, so the formation of GMP must be impaired in an as yet unknown manner Sequence alignments ofthe large GTP-binding domains of hGBP1, ... for the lack of GMP formation (see Fig S2) The similar hydrolytic activities ofthe a ⁄ b and C-terminally truncated forms of hGBP5 are in contrast to that ofthe analogous deletion mutant of ... mass (Mapp) in the GDP-bound form of approximately 70 kDa, most likely due to an elongated shape ofthe protein The same behavior is observed in the presence of GDPÆAlFx Because ofthe unexpected...
... the presence ofthe nucleotide These experiments indicate that the binding and/or hydrolysis of ATP not have any effect on the conformation of PfPDO, possibly because ofthe localization ofthe ... participation of both N-terminal and C-terminal active sites Refolding ofthe scrambled RNase in the presence of PDI was used as a positive control, and the absence ofthe recovery ofthe RNase activity ... using PDI However, the temperature ofthe assay, which is limited by the stability ofthe protein substrate RNase, is very far from the optimal growth temperature ofthe hyperthermopilic micro-organism...
... stability ofthe mutant Tms The mutations did not affect the structure ofthe protein as there was no significant alteration in the function or in the amount ofthe secondary structure However, the ... probe Thus, the analysis ofthe melting profile of human Tm was enhanced by the use of different probes The mutations affect the stability ofthe protein Heller et al ([19] and references therein) ... Summary ofthe thermodynamic parameters determined by DSC for the wild type (WT) and mutant Tms The uncertainties listed are the standard errors ofthe mean and included the uncertainty in the determination...
... features of its hysteretic properties Thus, the structures ofthe binary and ternary complexes of hPAH have provided a detailed picture ofthe protein contacts involved in the binding of biopterin cofactor ... and hydrogen bonding network ofthe pterin cofactor in the three structures Superpositions ofthe binary and ternary complexes of hPAH onto the crystal structure ofthe ligand-free dimeric rPAH ... (relative to the catalytic iron) and the hydrogenbonding network ofthe bound H4biopterin Moreover, the substrate induced repositioning ofthe cofactor also accounts for the release of its interaction...
... for the four substrates shown in the reaction It seems likely that under the appropriate conditions in the cell, the enzyme can catalyze either the synthesis or the degradation of trehalose Another ... emerged in the region suggesting a molecular weight of 220 kDa (Fig 3) These data suggest that the active TPS probably exists as a tetramer Propertiesofthe recombinant TPS The activity ofthe purified ... compared to the amount of NAD+ produced (nmol) in the coupled assay, as an indirect measure ofthe amount of trehalose-P synthesized However, for most ofthe studies described here, the anthrone...
... lines ofthe g ¼ 1.89 signal in the titrations in the presence of redox dyes was comparable to the titration in the absence of these dyes (Fig 9) The g ¼ 2.01 signal found in the absence of dyes ... the species causing it has a relaxation rate at 10 K which is ofthe same order of magnitude as that ofthe g ¼ 1.92 species We also note that the g-values and the temperature dependence ofthe ... 269) 6107 ofthe g ¼ 1.92 signal, whereas the gz ¼ 2.078 and the trough at g ¼ 1.887 form the gz and gxy lines ofthe g ¼ 1.89 signal At pH 7.0 both ofthe two gz lines as well as the two gxy...
... of these novel EPR signals to either the cofactor or the P-cluster has proven elusive due to the fact that both of these metal clusters present in the Fe-only nitrogenase are diamagnetic in the ... identical with the control spectrum in the absence of Rc1Mo Furthermore, with the exception ofthe classical S ¼ 3/2 signal ofthe MoFe protein at lower temperatures (< 12 K), no other signal was ... type of nitrogenase cluster [27–31] In view ofthe lack of analogous studies on the cofactor and the P-cluster ofthe MoFe protein from R capsulatus (Rc1Mo), as well as our intention to use the...