properties of polyphenol oxidases as proteins
Báo cáo khoa học: Comparison of functional properties of two fungal antifreeze proteins from Antarctomyces psychrotrophicus and Typhula ishikariensis ppt
... activity assay against ascomycetes We performed antifreeze activity assays on lL of the culture medium from each of 23 psychrotrophic ascomycetes (Fig 1) The assay was performed by observation of the ... TisAFP was dialyzed against 100 mm ammonium bicarbonate and then TH activity was measured 402 Protein analyses of AnpAFP The molecular mass of AnpAFP was measured using SDS ⁄ PAGE and MALDI-TOF ⁄ ... Antifreeze protein from ascomycetous fungus AnpAFP TisAFP AnpAFP B A.Acid Asx 10 20 AGLDLGAASX FGALAFEGVA AGPSAVPLGT AGNYVI LAST AGPTAVPLGT AGNYAI LAST AGPTAVPLGT AGNYAI LASA AGLDLGA ASX FGALAFEGVA 10...
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FOAMING PROPERTIES OF PROTEINS.pdf
... (FS) was observed around a gas flow rate (FR) of 20 ml/min and a pH of 5.0 At least three response surface graphs for each parameter [(percent of gas initially in the foam (Gi), volume of liquid ... significantly affected all measured foaming properties As protein content of the solution increased, each measured property improved as il- Table 4-Regression coefficients of surface response model ... condenserwithin 0S”C of the desiredtemperature (2-38°C) A speciaUy designed glassstopper(Fig 1) which permitted the introduction of gas and liquid was made in the Glass Shop (Dept of Physics,Cornell...
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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... hydro- Enzymatic properties of chitinase A from Vibrio carchariae lytic activity of chitinase A resulting in the production of a broad range of chitooligosaccharide products was measured simultaneously ... viscosity assay and HPLC-ESI MS suggested that the newly isolated chitinase acts as an endochitinase [25] We also reported isolation of the gene encoding chitinase A and functional expression of the ... When chitinase A was incubated with (GlcNAc)2, neither a decrease in (GlcNAc)2 nor an increase in GlcNAc was observed upon incubation up to 57 h (Fig 3A) In contrast, when (GlcNAc)3 was the substrate,...
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Báo cáo khoa học: Characterization of heme-binding properties of Paracoccus denitrificans Surf1 proteins doc
... oxidase biogenesis are a common cause of respiratory deficiencies that may lead to severe diseases such as Leigh syndrome In eukaryotic systems, an increasing number of protein factors has been associated ... efficient assembly of cytochrome c oxidase in yeast FEBS Lett 498, 46–51 13 Barrientos A, Korr D & Tzagoloff A (2002) Shy1p is necessary for full expression of mitochondrial COX1 in the yeast model of ... purification of Surf1 Heterologous expression of apo-Surf1 in E coli was performed in the absence of the Paracoccus heme a-synthesizing machinery as described previously [16] Expression of Surf1 proteins...
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Báo cáo " Effects of using wastewater as nutrient sources on soil chemical properties in peri‐urban agricultural systems " ppt
... problem of wastewater disposal on agricultural land is the potentially phytotoxic nature of organic wastes, mainly as a result of combination of factors such as high salinity or excess of ammonium ions, organic compounds ... assessing environmental risks of applying wastewater in agro‐ecosystems. The main objective of this paper was to quantify the effects of reuse of wastewater as nutrient sources by: ... 2.3. Soil sampling strategy and sample preparation For assessment of the impact of wastewater, soil samples were taken from the topsoil (0‐20 cm) of all study sites in peri‐urban areas of Hanoi (n=4) and Nam Dinh (n=8) using a soil ...
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Báo cáo khoa học: A novel plant protein disulfide isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins docx
... RNase A PDI activity was assayed by measuring RNase activity produced through the regeneration of the active form from reduced and denatured RNase A Reduced and denatured RNase A was prepared as ... Coomassie Brilliant Blue (B) Oxidative refolding activity of recombinant GmPDIM Activity was assayed by the measurement of RNase A activity restored through the regeneration of the active form of ... to that of the animal thiol-oxidoreductase P5 [7] The recombinant form of GmPDIM was active as it could refold RNase A that had been reduced and denatured The specific activity of GmPDIM was very...
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Báo cáo sinh học: " The Severe Acute Respiratory Syndrome (SARS)-coronavirus 3a protein may function as a modulator of the trafficking properties of the spike protein" docx
... expression of S will delay this cell-damaging effect and prevent the premature release of unassembled viral RNA It may also enhance virus packaging as it appears that the assembly of coronavirus ... trafficking properties of S Indeed, the dilysine endoplasmic reticulum retrieval signal, which is a different type of sorting signal from the YxxΦ motif, in the cytoplasmic tail of IBV was reported ... manifestations of SARS-CoV infection in contrast to the mild diseases caused by most coronaviruses, remains to be determined In addition, the exact roles of "accessory" proteins of SARS-CoV are...
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báo cáo hóa học:" The Severe Acute Respiratory Syndrome (SARS)-coronavirus 3a protein may function as a modulator of the trafficking properties of the spike protein" docx
... expression of S will delay this cell-damaging effect and prevent the premature release of unassembled viral RNA It may also enhance virus packaging as it appears that the assembly of coronavirus ... trafficking properties of S Indeed, the dilysine endoplasmic reticulum retrieval signal, which is a different type of sorting signal from the YxxΦ motif, in the cytoplasmic tail of IBV was reported ... manifestations of SARS-CoV infection in contrast to the mild diseases caused by most coronaviruses, remains to be determined In addition, the exact roles of "accessory" proteins of SARS-CoV are...
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Báo cáo hóa học: " Optical properties of as-grown and annealed InAs quantum dots on InGaAs cross-hatch patterns" doc
... cleaved into smaller (a) GaAs cap (b) In0.13Ga0.8 7As GaAs buffer (001)-GaAs [110] [1-10] Figure Structure of InAs QDs on InGaAs CHPs (a) Schematic cross-sectional diagram of the QDs on CHP structure ... optical properties of InGaAs/GaAs quantum dots J Cryst Growth 1998, 195:540-545 Leon R, Kim Y, Jagadish C, Gal M, Zou J, Cockayne DJH: Effects of interdiffusion on the luminescence of InGaAs/GaAs ... resulting from the state filling of each of the GS emerge, as expected Sample B (CHP) is basically an InGaAs quantum well (QW) sandwiched between the GaAs buffer and GaAs capping layers The lattice-mismatched...
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Báo cáo khoa học:" Properties of virion transactivator proteins encoded by primate cytomegaloviruses" doc
... rapid dispersal of Daxx from ND10 was not reflected in greater functional activity of S82; although S82 was approximately twice as active as pp71 in the expression assay, it was similar to B82 ... [24] For one set of cultures, histochemical staining for β-galactosidase was employed as a direct measure of continued gene expression mediated by the UL82 homolog A second set was superinfected ... of pp71 in activation of gene expression The intranuclear localization of the UL82 homologs was investigated by immunofluorescence after infection of HFFF2 cells at moi 0.1 with the in1374-based...
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Báo cáo khoa học: " Properties of virion transactivator proteins encoded by primate cytomegaloviruses" pptx
... rapid dispersal of Daxx from ND10 was not reflected in greater functional activity of S82; although S82 was approximately twice as active as pp71 in the expression assay, it was similar to B82 ... [24] For one set of cultures, histochemical staining for β-galactosidase was employed as a direct measure of continued gene expression mediated by the UL82 homolog A second set was superinfected ... of pp71 in activation of gene expression The intranuclear localization of the UL82 homologs was investigated by immunofluorescence after infection of HFFF2 cells at moi 0.1 with the in1374-based...
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Báo cáo y học: " Antiviral properties of two trimeric recombinant gp41 proteins" pot
... replication was monitored by measuring luciferase activity in cell extracts For each experiment, the inhibitory effect of recombinant proteins was compared to the effect of the same volume of solubilization ... artefactual decrease of the luciferase signal, the inhibitory effect of recombinant proteins was systematically compared to the same volume of buffer Figure shows the results of a typical experiment ... amount of recombinant proteins resulting in a 50% decrease in reporter activity compared to the value in the absence of inhibitor Luciferase and β-Galactosidase were used as reporters of infection...
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Properties of Convolution
... a phase that is a straight line, i.e., a linear phase Lastly, the frequency spectrum of a signal that is not symmetrical has a phase that is not a straight line, i.e., it has a nonlinear phase ... number The cutoff frequency of the filter is changed by making filter kernel wider or narrower If a low-pass filter has a gain of one at DC (zero frequency), then the sum of all of the points ... used as the input for the next system From the associative property, the order of the systems can be rearranged without changing the overall response of the cascade Further, any number of cascaded...
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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... release of drug was studied also in plasma and in PBS after addition of glutaraldehyde Pravastatin assay by UPLC A reversed phase UPLC method was developed and used throughout the study for pravastatin ... fragility was studied for each drug concentration In vitro releasing study The release of pravastatin as well as hemoglobin from carrier erythrocytes were determined as following, ml of packed ... using glutaraldehyde as a membrane stabilizer Also the figure shows the hemoglobin release The releasing curves of pravastatin show release phases, the first is rapid release phase and the second...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
... storage stability of PANs was studied in the aqueous medium, and the acute toxicity of PANs was evaluated in mice Morever, EPI was loaded into PANs and its pharmacokinetics was also assessed in rats ... the C-18 column was used and the mobile phase (0.02 M KH2PO4/CH3CN/CH3OH = 49:17:34 v/v/v) was delivered at a rate of 1 ml/min Sample (20 μl) was injected and the column effluent was detected with ... concentration of total EPI in plasma was 14.65 mg/L at 1 min after injection and then decreased to nearly undetectable levels after 24 h The maximum concentration (Cmax) of PA/EPI at 5 min was 9.80 mg/L,...
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Evaluation of Dredged Sediment as a Silt and Clay Source for Artificial Tidal Flats
... poly-aluminum chloride (PAC) as well as waste steel slag (WSS) (E5) A natural tidal flat (C3) at the same tidal level was chosen as a reference for monitoring benthic communities as well as physicochemical ... range of values observed in the five artificial tidal flats (E1―E5) of the seashore experiment Mountain sand (MS) was used because of its importance as the alternative to sea sand which is of limited ... survived in the mixtures of MS and DS as well as MS itself, however, its growth was dependent on the mixing ratio of DS At 5% silt and clay, the liability and shell length increase of R philippinarum...
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Thermal properties of the vernacular buildings envelopes: the case of the "Sassi di Matera" and "Trulli di Alberobello
... "Trulli of Alberobello" In the South of Italy, the sites of the "Sassi of Matera" (Figure 1a) (classified as humanity world heritage in 1993) and "Trulli of Alberobello" (Figure 1b) (classified as ... measurement of thermal conductance of two different typologies of building wall: a wall in blocks of calcarenite sandstone assembled with mortar, typical of the constructions of "Sassi of Matera," ... material of various natures 3.1 Masonry in Calcarenite sandstone of the "Sassi of Matera" The measurements were made on a 48-cm thick wall of blocks The external surface temperature of the masonry...
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Assessment of pretreatments and enzymatic hydrolysis of wheat straw as a sugar source for bioprocess industry
... hydrolysis of cellulose microfibrils to release glucose involves synergistic action of three enzymes: endo-glucanase, exo-glucanase and β-glucosidase Endo-and exo-glucanases are commonly referred to as ... to the profits of a biorefinery The use of oxidative pretreatments that rely on degradation of this component would not be feasible as great amounts of this valuable commodity would be wasted Besides ... efficient xylan hydrolysis such as α-arabinofuranosidases and α-Larabinases that release arabinan [31], α-glucuronidases that release glucoronic acids, acetyl xylan esterases that hydrolyze acetylester...
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Effects of time of heatsetting on the tensile properties of ingeo™ poly (lactic acid) (PLA) fabric
... % Increasing time of heatsetting had effect on tensile properties of PLA at varying times of 30s to 90s at temperature of 130°C Optimum effect on tensile properties occurred at times of 30 – ... Figure Effect of time of heatsetting on the linearity of load extension in weft direction of knitted Ingeo™ Poly (lactic acid) Figure Mean effect of time of heatsetting on the linearity of load extension ... (PLA) fabric Conclusion The effect of time of heatsetting on tensile properties of knitted Ingeo™ Poly (lactic acid) (PLA) was determined using the KES-FB system of fabric testing Tensile parameters...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose