properties of a computed statistic

Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx

Ngày tải lên : 19/02/2014, 17:20

... Correia IJ, Paquete CM, Coelho A, Almeida CC, Catarino T, Louro RO, Frazao C, Saraiva LM, ˜ Carrondo MA, Turner DL et al (2004) Proton-assisted two-electron transfer in natural variants of tetraheme ... da Silva JJR, Amorim MTS, Cabral MF, Chaves S & Costa J (1991) Dissociation constants of Bronsted acids in D2O and H2O: studies on polyaza and polyoxa–polyaza macrocycles and a general correlation ... The half-height widths of the NMR signals were used as a measure of the uncertainty of each NMR data point and an experimental uncertainty of 2% was assumed for the experimental points of the...

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Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx

Ngày tải lên : 21/02/2014, 15:20

... explain the higher antibacterial activity on Gram-negative bacteria for parabutoporin compared to opistoporin Also with magainin analogs, an increase in antibacterial activity against Gram-negative ... (34 amino acids) [19], peptides consisting of amino acids 7–22 of parabutoporin (an a- helical part having the four amino acids LAKK identical to mastoparan) and of the ﬁrst 28 amino acids of the ... being least active against S marcescens Mastoparan is far less active in inhibiting growth of Gram-negative bacteria and melittin is only active against three of the Gram-negative bacteria at the...

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Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf

Ngày tải lên : 07/03/2014, 11:20

... were made with PYMOL [47] able sequences, some bacterial family 19 chitinases have catalytic domains that are at least as large as those of the plant enzymes and that may contain at least six ... role of these glutamates was demonstrated by site-directed mutagenesis Table shows that all mutants had greatly reduced catalytic activity Some detectable activity was still left upon mutation of ... Haemophilus inﬂuenzae, and Pseudomonas aeruginosa Although many plant family 19 chitinases are known, crystal structures are available for only two of these [9,15–17] The ﬁrst structure of a...

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Báo cáo khoa học: Dissociation/association properties of a dodecameric cyclomaltodextrinase Effects of pH and salt concentration on the oligomeric state pot

Ngày tải lên : 07/03/2014, 12:20

... mutant, 5Â-TCTGCTGCAGCA GGGTGTT GAGAAGCGCTGGATG-3Â (forward) and 5Â-CATCCAG CGCTTCTCAACACCCT GCTGCAGCAGA-3Â (reverse); for the H539V mutant, 5Â-CGACAAGGCGGGCGTC ACGTTA ACGCTGCCTGTCC-3Â (forward) ... dissociation of dodecameric CDase I-5, sedimentation equilibrium 110 Fig Apparent molecular mass of CDase I-5 at various pH values determined by analytical ultracentrifugation analysis analysis was performed ... the enzymatic properties of CDase I-5 was examined at various concentrations of KCl First, the role of KCl in dissociation of CDase I-5 was investigated by analytical ultracentrifugation As the...

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Báo cáo khóa học: Localization, puriﬁcation and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot

Ngày tải lên : 07/03/2014, 14:20

... These data indicated that the fractionation procedure was satisfactory and that the tetrathionate hydrolase is a periplasmic enzyme Puriﬁcation of the tetrathionate hydrolase from tetrathionate-grown ... conditions at temperatures of up to 80 °C (data not shown) The interference of ammonium sulfate with the HPLCassay of tetrathionate was also tested The height of the tetrathionate peak decreased and a ... cell fractions Distribution and activity of assayed enzymes are given as a percentage of the total protein concentration and total activity in the cell free extract Tetrathionate hydrolase activity...

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Báo cáo khoa học: Puriﬁcation and properties of a new S-adenosyl-Lmethionine:ﬂavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot

Ngày tải lên : 08/03/2014, 08:20

... of molecular mass markers are indicated in kDa F 4¢-OMT activity towards the different assayed substrates, transformation products and kinetic analysis The enzyme became inactive when the assayed ... B and eluted with 80 mL of a 0–0.3 M linear gradient of NaCl in buffer B, at a flow rate of 0.4 mLÆmin)1 Fractions containing an F 4¢-OMT activity were pooled, dialyzed and concentrated as above ... was a mixture of 0.05 M NaPi buffer, pH 3, and acetonitrile (6 : 1, v/v); separation was performed isocratically, at a flow rate of mLÆmin)1, and the volume of injected samples was 10 lL The amounts...

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Báo cáo Y học: Assignment of molecular properties of a superactive coagulation factor VIIa variant to individual amino acid changes potx

Ngày tải lên : 08/03/2014, 09:20

... subtracted and the net amount of factor Xa generated was derived from a standard curve The kinetic parameters of FX activation were calculated using GRAFIT 4.06 (Erithacus Software, Ltd) The rates ... (Amersham Pharmacia Biotech) Activity and inhibition assays The enzymatic activity and inhibition rates of the FVIIa variants were measured as described [9] using an assay buffer containing 50 ... to achieve an amidolytic activity similar Table Enzymatic activity of free FVIIa variants All values are means SD (n ẳ 3) The amidolytic activity is given as the ratio between the activity of...

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Báo cáo khoa học: Ligand binding and antigenic properties of a human neonatal Fc receptor with mutation of two unpaired cysteine residues docx

Ngày tải lên : 16/03/2014, 06:20

... Sundaresan G, Subbarayan M, Carter NH, Ikle DN, Yazaki PJ, Chatziioannou AF, Gambhir SS et al (2005) Tailoring the pharmacokinetics and positron emission tomography imaging properties of anti-carcinoembryonic ... I-related receptor consisting of a heavy chain (HC) with three ectodomains (a1 , a2 and a3 ), a transmembrane part and a short intracellular signaling tail Like MHC class I HC, the FcRn counterpart ... human HC variants Table S3 Numbers of cysteine residues of FcRn HCs across speciesa Table S4 Numbers of cysteine residues of nonclassical MHC class I HCa This material is available as part of the...

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sensitivity properties of a novel no2 gas sensor based on mesoporous wo3 thin film

Ngày tải lên : 20/03/2014, 13:07

... Tainan, Taiwan, 2001 His major research has related to mesoporous materials, semiconductor gas sensor, and nanomaterials Min Hsiung Hon is a professor in the Department of Materials Science and ... rhodium–alumina catalyst, J Catal 43 (1976) 131–142 225 at National Cheng Kung University, Tainan, Taiwan His major research has related to mesoporous materials, and battery materials Jiann Shieh received ... oxidizing and reducing gases The stability of the sensing characteristics was examined several times in a week It was found that the initial resistance was approximately maintained, but with a slightly...

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synthesis, electrical measurement, and field emission properties of a-fe2o3 nanowires

Ngày tải lên : 20/03/2014, 13:08

... is a constant for all of the as-produced a- Fe2O3 NWs Using the mean length and radius of the NWs, RNW (or Roverall) of samples A, B, C, and D was calculated as 1.25 1011, 1.4 1011, 1.4 1011, and ... Nanoscale Res Lett (2008) 3:330–337 331 thermally stable, resistant to oxidation, and have a high aspect ratio, a- Fe2O3 NWs are a candidate emitters for FE applications It has been reported ... emission of large-area and graphitized carbon nanotube array on anodic aluminum oxide template J Appl Phys 93, 5602–5605 (2003) doi:10.1063/1.1564882 J.M Bonard, M Croci, I Arfaoui, O Noury, D Sarangi,...

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Báo cáo khoa học: Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa doc

Ngày tải lên : 23/03/2014, 10:20

... m)1Æcm)1) after dilution in water AMA was from Sigma Aldrich (Milan, Italy) All other reagents, purchased from different companies, were of analytical grade Rabbit serum raised against HNE–protein adducts ... considered as an extracellular counterpart of the chemical inactivation of HNE that occurs intracellularly via GST and other enzymes that 5138 are abundantly expressed in nasal mucosa [22] Furthermore, ... concentration of AMA Competitions between HNE and AMA are shown for porcine (C) and bovine (D) OBP Protein samples were incubated with a ﬁxed saturating amount of AMA and increasing HNE Each point...

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Báo cáo Y học: Puriﬁcation and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans doc

Ngày tải lên : 23/03/2014, 21:20

... catalyzing this reaction at high speciﬁc rates The enzyme preparation was found to be composed of a CO dehydrogenase and a hydrogenase Amino-terminal sequence analysis allowed the assignment of ... indicated, the enzyme was activated with mM Ti(III)citrate prior to the assay and mM Ti(III)citrate was added to the assay mixture The gas phase was 100% CO (1 · 105 Pa) or as indicated The reaction ... was started by the addition of enzyme The solution was stirred vigorously with a magnetic bar At 1.5-min intervals, samples from the gas phase were withdrawn, and H2 was quantiﬁed after separation...

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Báo cáo khoa học: Protein dissection enhances the amyloidogenic properties of a-lactalbumin potx

Ngày tải lên : 30/03/2014, 16:20

... EDTA, the LA variants Th1-LA and desb-LA adopt a conformation similar to the MG state displayed by intact LA at low pH, retaining a native-like a- helical content (Fig 2A, B) Furthermore, Th1-LA and ... during aggregation at 35 °C pH 2.0 The extent of cleavage was calculated by integration of the area of the peaks in the RP-HPLC chromatograms of protein samples analysed at different time intervals ... increases after incubation of LA at pH 2.0 for 6–10 h We may infer that LA, at the initial stages of aggregation, exists in a more expanded and ﬂexible conformational state and, for this reason,...

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báo cáo hóa học: " Psychometric properties of a single-item scale to assess sleep quality among individuals with fibromyalgia" potx

Ngày tải lên : 18/06/2014, 18:20

... medication up to and including Day1 If fewer than scores are available then baseline consists of all scores that are available Page of (page number not for citation purposes) Health and Quality of ... clinical trials During the baseline phase, study patients had to have an average daily diary pain score of at least (within the last days) on a numeric rating scale ranging from ("no pain") to ... 1056 and 91.0% in study 1077) (Table 1) In study 1056, the mean age of patients was 48.8 years and the average duration of FM was years In study 1077, the mean age of patients was 50.1 years and...

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Báo cáo hóa học: " Nanomechanical properties of a-synuclein amyloid fibrils: a comparative study by nanoindentation, harmonic force microscopy, and Peakforce QNM" potx

Ngày tải lên : 21/06/2014, 04:20

... uncertainty of approximately 39% of the average measured value In addition to these errors, experimental data are also influenced by the expected statistical variation due to heterogeneity of a large sample ... was calibrated with a reference sample (model PS-LDPE, Veeco, Santa Barbara, CA, USA) [20] Since HarmoniX assumes a spherical tip that indents an infinitely large and thick flat elastic surface, ... increased data throughput [18-20] Two commercially available approaches are PeakForce QNM and Harmonic force microscopy or HarmoniX (Veeco, Santa Barbara, CA, USA) PeakForce QNM is based on the...

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báo cáo hóa học:" Structural and optical properties of a radio frequency magnetron-sputtered ZnO thin film with different growth angles" pdf

Ngày tải lên : 21/06/2014, 17:20

... Torr A pre-sputtering treatment was performed to clean the target surface for 10 using argon plasma A distance between the target center and the sample substrate was kept at 9.5 cm, and we manually ... H-SH performed data acquisitions and analysis All authors read and approved the final manuscript Average (400~800 nm) 25 20 15 Competing interests The authors declare that they have no competing ... with a 0° growth angle has the lowest reflectance of 10.81% at 418 nm The 15° angled film has the best condition on average and low values The transmittance and reflectance are slightly changed...

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Báo cáo hóa học: " Synthesis, Electrical Measurement, and Field Emission Properties of a-Fe2O3 Nanowires" potx

Ngày tải lên : 22/06/2014, 01:20

... is a constant for all of the as-produced a- Fe2O3 NWs Using the mean length and radius of the NWs, RNW (or Roverall) of samples A, B, C, and D was calculated as 1.25 1011, 1.4 1011, 1.4 1011, and ... Nanoscale Res Lett (2008) 3:330–337 331 thermally stable, resistant to oxidation, and have a high aspect ratio, a- Fe2O3 NWs are a candidate emitters for FE applications It has been reported ... emission of large-area and graphitized carbon nanotube array on anodic aluminum oxide template J Appl Phys 93, 5602–5605 (2003) doi:10.1063/1.1564882 J.M Bonard, M Croci, I Arfaoui, O Noury, D Sarangi,...

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Báo cáo hóa học: " Research Article Monotonic and Logarithmically Convex Properties of a Function Involving Gamma Functions" pdf

Ngày tải lên : 22/06/2014, 02:20

... Analysis, Cambridge Mathematical Library, Cambridge University Press, Cambridge, UK, 1996 B.-N Guo and F Qi, “Inequalities and monotonicity for the ratio of gamma functions,” Taiwanese Journal ... Diﬀerential- und Integralrechnung II, VEB Deutscher Verlag der Wissenschaften, Berlin, Germany, 1966 M Abramowitz and I A Stegun, Handbook of Mathematical Functions with Formulas, Graphs, and Mathematical ... “Generalizations of Euler numbers and polynomials,” International Journal of Mathematics and Mathematical Sciences, vol 2003, no 61, pp 3893–3901, 2003 14 F Qi and B.-N Guo, A new proof of complete...

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Structural characterization and study of immunoenhancing properties of a glucan isolated from a hybrid mushroom of Pleurotus florida and Lentinula edodes pot

Ngày tải lên : 28/06/2014, 11:20

... Structural characterization and study of immunoenhancing properties of a glucan isolated from a hybrid mushroom of Pleurotus florida and Lentinula edodes Praloy K Majia, Ipsita K Sena, Birendra Beherab, ... prepared with standard dextran PS-I was hydrolyzed with 2M trifluroacetic acid and then alditol acetate23 was prepared for GLC analysis GLC analysis of alditol acetate of hydrolyzed product of ... and then alditol acetate was prepared to know the linkages of sugar moieties The GLC-MS analysis of the partially methylated alditol acetate of PS-I revealed the presence of 1,3,5,6-tetra-O-acetyl-2,4-di-O-methyl-glucitol,...

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Structural characterization and study of immunoenhancing properties of a glucan isolated from a hybrid mushroom of Pleurotus florida and Lentinula edodes potx

Ngày tải lên : 28/06/2014, 11:20

... Structural characterization and study of immunoenhancing properties of a glucan isolated from a hybrid mushroom of Pleurotus florida and Lentinula edodes Praloy K Majia, Ipsita K Sena, Birendra Beherab, ... prepared with standard dextran PS-I was hydrolyzed with 2M trifluroacetic acid and then alditol acetate23 was prepared for GLC analysis GLC analysis of alditol acetate of hydrolyzed product of ... and then alditol acetate was prepared to know the linkages of sugar moieties The GLC-MS analysis of the partially methylated alditol acetate of PS-I revealed the presence of 1,3,5,6-tetra-O-acetyl-2,4-di-O-methyl-glucitol,...

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