propeptides of type i procollagen
Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc
... Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipV Cloning of sipV Cloning of sipV Cloning of sipV ... Cloning of sipV Cloning of sipV Cloning of sipV Construction of pOpacVh Construction of pOpacVh Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW ... cut with either PstI and EcoRI in case of sipV or EcoRI-SacI in case of sipW and ligated into corresponding sites of pUC18 DNA The ligation mixes were used for PCR with oligo nucleotides Uni1 or...
- 12
- 595
- 0
Báo cáo khoa học: Promoters of type I interferon genes from Atlantic salmon contain two main regulatory regions docx
... role in the activation of the salmon IFN promoter, but it cannot act alone to initiate transcription A hallmark of mammalian IFN-a ⁄ b is their rapid induction by virus infection mainly because of ... shown (Fig 7) PDTC is a metal chelator with antioxidant properties which specifically inhibits NFjB-induced pathways [39] Secondly, partial inhibition of promoter activity by 2-AP (Fig 7), indicates ... Atlantic salmon A role for NFjB in the activation of PR -I was further supported by two different inhibitor experiments First, dose-dependent inhibition of promoter activity by the NFjB inhibitor...
- 14
- 379
- 0
Báo cáo khoa học: A comparative study of type I and type II tryparedoxin peroxidases in Leishmania major pot
... TryX indicating that formation of an intramolecular disulde is important for interaction with TryX In contrast, this mutant displayed signicant activity with dithiothreitol (65% of wild -type) suggesting ... reduced with 10 mm dithiothreitol or oxidized with two equivalents of hydrogen peroxide, respectively Dithiothreitol, trace amounts of oxidized dithiothreitol or hydrogen peroxide did not inuence ... partial mobility shifts under reducing and oxidizing conditions by SDS PAGE with some higher aggregate formation evident following peroxide treatment (Fig 7) Abrogation of the mobility shift is...
- 16
- 483
- 0
Báo cáo hóa học: " Wild type measles virus attenuation independent of type I IFN" pdf
... ELISA Infection with UV-inactivated virus did not induce antibody production, strongly suggesting that generation of antibodies requires initial replication of G954-V13 after intranasal infection ... http://www.virologyj.com/content/5/1/22 Figure Adaptation to a specific cell type limits replication of MV Adaptation to a specific cell type limits replication of MV Replication kinetics of MVs in PBMCs ... infection and type I IFN addition] However, high doses of type I IFN could inhibit the G954-V13 strain infection [(F): hours between infection and type I IFN addition] Ed-Zagreb infections of both...
- 12
- 271
- 0
Báo cáo toán học: " On Systems of Quasivariational Inclusion Problems of Type I and Related Problems" potx
... a solution of one of the above systems, under some additional conditions, is also a solution of some other systems of quasi-optimization problems, quasi-equilibrium problems, quasivariational ... of this paper is to prove some new results on the existence of solutions to systems concerning the following quasivariational inclusions (UQVIP), Upper quasivariational inclusion problem of type ... short) if y − x ∈ C for every y ∈ A The set of ideal minimal points of A is denoted by IMin(A/C) (ii) x ∈ A is an efficient (or Pareto–minimal, or nondominated) point of A w.r.t C if there is no...
- 18
- 292
- 0
Dynamic studies of type i and type II cadherins EC domains
... chemical modification was not utilized in the unbinding experiments are i) it shows a high probability of non-specific binding, which cannot be distinguished from cadherin interactions ii) it ... cadherins form cadherin-catenin complex with catenins and p120, no vinculin is recruited B) In the released state, the vinculin binding site in α-catenin is inhibited by a putative inhibitory domain ... interaction Biotin-NTA Strepavidin-NTA NH2-NTA strepavidin BSA Strepavidin Biotin Biotin-strepavidin interaction SVA NH2 NTA SiO2 Figure 2.2 Chemical modification method for AFM unbinding experiments...
- 128
- 290
- 0
DISCOVERY OF NOVEL REGULATORS OF TYPE i INTERFERON RESPONSE
... the escalating of infection inside the host (Schoggins & Rice, 2011) 15 The ubiquitination mediated regulation of cell signalling The ubiquitin ligases and ubiquitination Ubiquitination is a post ... translational modification of proteins Ubiquitination regulates multiple aspects of protein functioning such as stability, signalling complex formation or dissociation etc Ubiquitination is the ... potent antiviral type- I interferons While ubiquitination is well known to regulate interferon response, a genome-wide understanding of the role of ubiquitin ligases in TLR3 signaling is yet to...
- 73
- 253
- 0
Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx
... compilation ª 2010 FEBS 3783 SR-BI induction by KLF4 T Yang et al Fig DNA binding activity and transcription activity of KLF4 to the KLF binding element of hSR-BI promoter in PMA-differentiated ... identified that induction of KLF4 by HDL3 promoted the expression of hSR-BI It has been shown that disruption of SR-BI in mice impairs HDL-cholesterol delivery to the liver and induces susceptibility ... carcinogenesis In normal conditions, the expression of KLF4 mRNA is most abundant in the colon and skin in mice, whereas expression of KLF4 is decreased in intestinal adenomas of multiple intestinal...
- 9
- 516
- 0
Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx
... immunoprecipitated with an anti-FGFR3 serum then immunoblotted with anti-ubiquitin and anti-FGFR3 sera (D) Disabling the c-Cbl ubiquitylating activity does not affect the ubiquitylation of the wild -type, ... with chloroquine as indicated Lysates were immunoprecipitated and processed for immunoblotting with anti-ubiquitin and anti-FGFR3 sera (C) Ubiquitylation of the wild -type receptor is increased by ... FGFR signals via different signalling pathways resulting in the regulation of many cellular processes including proliferation, differentiation, migration and survival [1–4] Dominant mutations in...
- 16
- 573
- 0
Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf
... substantial sequence similarities as indicated by six distinct regions with conserved amino acids [26] For instance, LepB from Escherichia coli is 323 amino acids long and exists only as a single ... analyzed by ESI-QTOF MS (Fig 3) in the positive ion mode, to get high quality fragmentation pattern for peptide sequencing Fifteen 2893 Signal peptidase I activity in M pneumoniae I Catrein et al ... labeled within the intact protein at its a-amino group and represents the amino terminal amino acid of P40 The efficiency of this reaction was quite high, as judged from the signal intensities of the...
- 9
- 559
- 1
Báo cáo khoa học: Enzymatic investigation of the Staphylococcus aureus type I signal peptidase SpsB – implications for the search for novel antibiotics ppt
... inhibitor, v0 is the initial velocity in the absence of inhibitor but with (2%) dimethylsulfoxide The IC50 value was determined by tting the percent inhibition versus inhibitor concentration using ... ve of these amino acids are perturbed by substrate binding [17], highlighting their signicance In SpsB, it is also likely that one or more of the amino acids immediately preceding the catalytic ... concentration) Percent inhibition was calculated using the equation [(1 (vi v0)] ã 100, where vi is the initial ab ỵ cxd b ỵ xd 2ị Activity at varying pH For determination of optimum pH, the in vitro...
- 13
- 464
- 0
Báo cáo khoa học: Involvement of lysine 1047 in type I collagen-mediated activation of polymorphonuclear neutrophils doc
... because it induced a dose-dependent inhibition of ROS production, indicating the impairment of the interaction This inhibitory effect was independent of any direct scavenger effect of 3230 B Fig Influence ... (contained within the cyclic peptide CTRKKHDNAQC derived from jararhagin disintegrin) is essential for binding to the I domain of a2 integrins [24] Similarly, members of the ‘lysine–threonine–serine ... A, Wiman B & Pap S (2003) Identification of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin Eur J Biochem 270, 2023–2029 Panizzi P,...
- 10
- 519
- 0
Báo cáo khoa học: Differential interactions of decorin and decorin mutants with type I and type VI collagens pptx
... chips and with 0.3 M NaCl in the case of immobilized type VI collagen To form a complex consisting of decorin, type I and type VI collagen, type VI collagen was first immobilized After binding of ... cells WT, Wild -type Type I collagen binding Type Type Type Type Fig Surface plasmon resonance measurement of immobilized pepsin digested type VI collagen (A) Interaction with wild -type decorin core ... collagen, white cylinder (A) Interaction involving dimerization of decorin and binding of both collagens to the inner surface of decorin (B) Interaction of type I collagen with the inner surface of the...
- 10
- 458
- 0
Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
... respectively The Table Kinetic properties of the wild -type and N130D variants of human arginase I The inhibitors used were ornithine (orn) and guanidinium chloride (Gdn) Substrate Arginine Agmatine ... compilation ª 2006 FEBS ´ R Alarcon et al Agmatinase activity of the N130D mutant arginase I Fig Inhibition of wild -type and N130D variants of human arginase I by agmatine (A) and putrescine ... ability to act on the decarboxylated derivative of arginine However, the possibility of interaction with residues such as Asp193, proposed as ligands for the a-amino group of arginine in arginase...
- 7
- 303
- 0
Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf
... metabolism The reduction in d-amino acid oxidase expression in the kidney proteome is interesting, in view of the contribution of this enzyme to glyoxylate production from glycine Thus, it could ... 13278579 16740777 gi: gi: gi: gi: gi: gi: gi: gi: gi: 14789706 387083 12850132 13542872 12805529 15488762 15488685 109571 18043201 gi: 81894107 gi: gi: gi: gi: gi: gi: gi: gi: gi: gi: NCBI no 10 10 10 ... peroxisomal fragility With this limitation, our fractionation method was mainly useful as a simple way to reduce the complexity of the proteome, facilitating the differential expression analysis...
- 9
- 481
- 0
Báo cáo khoa học: Type I receptor binding of bone morphogenetic protein 6 is dependent on N-glycosylation of the ligand pdf
... derived from E coli expression is inactive in these cells, probably due to its lack of binding to ActR -I (B) Signaling of BMP-6 and BMP-7 via ActR -I is shown in the inhibition of proliferation in ... koff > 10)1Æs)1), impeding the analysis of the dissociation rate and thus requiring analysis of the equilibrium binding ActR -I, is inactive in cell-based assays, as would be expected if ActR -I ... association and dissociation rates of the interaction; analysis of dose dependency of equilibrium binding yields higher (three-fold) values for KD(eq), as real equilibrium binding cannot be achieved,...
- 12
- 395
- 0
Báo cáo Y học: In vitro gene therapy of mucopolysaccharidosis type I by lentiviral vectors pdf
... Prof Elizabeth F Neufeld, UCLA School of Medicine, for providing us with the pBSIIKS-hIdu plasmid and anti-IDUA antibodies We thank the ÔLaboratorio di Diagnosi Pre-Postnatale Malattie Metaboliche ... high levels of IDUA expression in recipient cells in vitro and may also provide sufficient expression in deficient cells and tissues in vivo to similarly reduce or prevent storage accumulation in ... number of Table a-L-Iduronidase activity in MPS I fibroblasts after transduction with lentiviral vector Increasing amounts of IDUA vector were added to MPS I fibroblasts as indicated Cells, in triplicate...
- 8
- 526
- 0
báo cáo hóa học: " Clinical usefulness of the screen for cognitive impairment in psychiatry (SCIP-S) scale in patients with type I bipolar disorder" doc
... present investigation was designed to assess the sensitivity, reliability and validity of the SCIP for detection and quantification of cognitive impairment in euthymic patients suffering from a type ... Conclusion: With minimal training, physicians obtained a reliable and valid estimate of cognitive impairment in approximately 15 minutes from an application of the SCIP to type I BD patients Background ... baseline divided by the standard deviation of the baseline visit The validity of the SCIP for a quantification of cognitive impairment in bipolar disorder was examined with an assessment of construct...
- 10
- 631
- 0
Báo cáo hóa học: " Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15)" potx
... (Figure 1A) This finding is in keeping with the findings of NS1/NS2 antagonism of type I IFNs [4,46,47] and suggests the possibility that type I IFN antagonism is linked to NS1/NS2 induction of SOCS1 ... effective in regulating type I IFN expression SOCS1 can directly associate with high affinity to all Janus kinases (JAKs) directly inhibiting their catalytic activity, while SOCS3 functions in part ... virus infection of MLE-15 cells, it is unlikely NS1/ NS2 has a role in modifying ISG15 The finding in this study that G protein expression inhibits IFNβ and ISG15 protein expression is consistent...
- 11
- 435
- 0
Báo cáo khoa học: "Topology of Scavenger Receptor Class B Type I (SR-BI) on Brush Border Membrane" docx
... cys in mouse SR-BI; unknown in rabbit SR-BI There must be a lot of inter- or intra-disulfide bridges which might be important for maintaining the three dimensional structure of SR-BI for binding ... rabbit BBMV is located in extracellular domain, and SR-BI is only functional when it is part of dimeric forms which rationalize the previously found cooperative nature of the binding interaction ... SR-BI is only functional when it is part of dimeric forms which rationalize the previously found cooperative nature of the binding interaction Our previous study [10] showed that binding of apo...
- 8
- 162
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose