production and partial purification of glycosidases obtained by solid state fermentation of grape pomace using aspergillus niger 10
studies of pathogenesis-related proteins in the strawberry plant partial purification of a chitinase-containing protein complex and analysis of an osmotin-like protein gene
... reactions were stopped by addition of 200 μL of 10% trichloroacetic acid The undigested substrate was pelleted by centrifugation at 15,000 g for 10 Avoiding the pellet, 220 μL of supernatant was ... PARTIAL PURIFICATION OF A CHITINASE-CONTAINING PROTEIN COMPLEX IN THE STRAWBERRY PLANT……………………………32 ISOLATION OF AN OSMOTIN-LIKE PROTEIN GENE FROM STRAWBERRY AND ANALYSIS OF THE RESPONSE OF THIS ... through lignification, suberization, and callose deposition; production of phytoalexins which are secondary metabolites, toxic to bacteria and fungi; and synthesis of pathogenesis-related (PR) proteins...
- 119
- 307
- 0
Báo cáo khoa học: Purification and partial characterization of seven glutathione S -transferase isoforms from the clam Ruditapes decussatus pptx
... molecular mass and the GST-specific enzymatic activities of purified GST isoforms of R decussatus All purified isoforms catalyzed the conjugation of GSH with CDNB, whereas isoforms 1-1, 2-2 and 4-4 had ... velocity and E0 is the initial concentration of enzyme SDS/PAGE Purification of GSTs All procedures were performed at 0–4 °C Fifty milliliters of the supernatant were dialyzed with a cut-off of 10 kDa ... parameters between those of GST isoforms 3-3 and 6-6, seemed to have the properties of each of its subunits Immunoblotting Fig RP-HPLC of each affinity fraction (C1E1, C2E1, C1E2 and C2E2) Protein samples...
- 8
- 338
- 0
Enhanced production of pectinase by Bacillus sp. DT7 using solid state fermentation docx
... effect of different solid substrates on pectinase production was studied by using a variety of solid substrates (wheat bran, rice bran, apple pomace) The original moisture content of the apple pomace ... coagulans in solid state fermentation Process Biochem 30, 305–309 Gessesse, A., Mamo, G., 1999 High level xylanase production by an alkalophilic Bacillus sp by using solid state fermentation ... mutants of Aspergillus niger adapted either to solid- state fermentation or submerged fermentation Enzyme Microb Technol 21, 25–31 Pandey, A., Soccol, C.R., Mitchell, D., 2000 New developments in solid...
- 4
- 591
- 1
Báo cáo y học: "Increased circulating levels and salivary gland expression of interleukin-18 in patients with Sjögren''''s syndrome: relationship with autoantibody production and lymphoid organization of the periductal inflammatory infiltrate" pptx
... histological evaluation of the number of foci, the presence of IL-18 in inflammatory foci and ducts, the degree of organization of the mononuclear aggregates, and the presence of germinal centers ... autoantibody production and clinical parameters of this condition Second, since SS is mainly a localized disorder, we examined the expression and distribution of IL-18 in salivary glands of SS Third, ... capable of directly inducing expression of proinflammatory cytokines such as TNF-α and IL-1β in mature Th1 cells, macrophages, and natural killer cells [1214], to up-regulate production of both CC and...
- 10
- 668
- 0
Báo cáo y học: " Identification of super-infected Aedes triseriatus mosquitoes collected as eggs from the field and partial characterization of the infecting La Crosse viruses" pot
... infection of Anopheles gambiae Proceedings of the National Academy of Sciences of the United States of America 2004, 101 :17240-17245 10 Campbell CL, Black WC, Hess AM, Foy BD: Comparative genomics of ... diluted 10- 1 to 10- 6 and 200 μl of each virus dilution was added to one well and incubated at 37°C for hour The virus inoculum was then removed and ml of overlay (1% agar in Medium 199, 10% fetal ... titers of SI+ mosquitoes were determined by endpoint titration in Vero E6 cells [33] The mosquito homogenates were serially diluted 10- 1 - 10- 6 and 200 μl of each dilution was added to one well of...
- 27
- 308
- 0
purification and characterizations of phytase enzyme form aspergillus niger
... concentration was measured by Bradford method (1976) and phytase activity was determined by the method of Heinonen and Lahti (1980) 2.2.1.3 Purification of phytase by cation exchange chromatography ... hexakisphosphate) is the primary storage form of phosphorus in plants They occupy about 1-3% of the seeds of grains and legumes, and 60-80% of the total amount of plant phosphorus (Nelson, 1967) Phosphorus ... continuation of this research, the thesis on "Purification and characterizations of phytase enzyme from Aspergillus niger" has been performed with the aim to obtain purifed phytase and some its...
- 25
- 296
- 0
Tài liệu Báo cáo khoa học: "Fast and Robust Part-of-Speech Tagging Using Dynamic Model Selection" pptx
... achieved near state -of- the-art results Shen et al 365 (2007) presented a tagging approach using guided learning for bidirectional sequence classification and showed current state -of- the-art results.3 ... algorithm using bidirectional dependency networks, and showed the best contemporary results Gim´ nez e and M` rquez (2004) used one-pass, left-to-right a and right-to-left combined tagging algorithm and ... index of the current word, f : SW, m: LSW, p: POS, a: ambiguity class, c∗ : character sequence in wi (e.g., c:2 : the 1st and 2nd characters of wi , cn−1: : the n-1’th and n’th characters of wi...
- 5
- 455
- 0
Direct growth of amorphous silica nanowires by solid state transformation of sio2 films
... by thermal decomposition of Fig FESEM photographs of a-SiONWs nanowires grown on the substrates after annealing at 105 0 °C in: (a) H2 (200 sccm) for 10 followed by CH4 :H2 (10: 200 sccm) for 10 ... limited by introducing ammonia (NH3 ) into the system to suppress the decomposition of TiN films The Ni islands act as a nucleation site for the a-SiONW growth, and expedite the reduction of TiN by ... formation of a titanium oxide (TiOx ) phase by removal of oxygen from the silica layer [9] The reduction of TiN seems to be a critical factor in the growth kinetics of a-SiONWs The growth of silica...
- 5
- 384
- 0
Monetary Policy in the Eurozone: Evaluating the European Central Bank’s interest rate decisions and the needs of member states using a Taylor rule ppt
... for Spain and Ireland, causing a substantial acceleration of their inflation and threatening their competitiveness Such disparities of demand conditions will undoubtedly persist 40 Lee and Crowley ... Challenge of the Enlargement of Euroland.” (HM Treasury 2002) 66 27 Srivangipuram additional concerns of the structure of the Economic and Monetary ... coefficients and constant (of 1) stated above through the assumption that the natural rate of interest is 2%, using aπ = and ay = In addition, rather than grouping countries into the core and peripheral,...
- 41
- 513
- 0
Báo cáo hóa học: " Dance-the-Music: an educational platform for the modeling, recognition and audiovisual monitoring of dance steps using spatiotemporal motion templates EURASIP Journal on Advances in Signal Processing 2012," doc
... one, instead of all eight windows at once (see Figure 10) The position and rotation of the window is thereby totally dependent of the position and rotation of the user at the beginning of each new ... Example of the internal mechanism of the template matching algorithm It represents the result of the comparison of a dance figure consisting of eight steps (defined each by 100 samples) performed by ... training data consists of pairs of input objects and a desired output value In our case, the training data consists of a set of p repetitive cycles of a specific dance figure of which we process the...
- 42
- 325
- 0
Báo cáo y học: "Annotating conserved and novel features of primate transcriptomes using sequencing" pdf
... Khaitovich P: Intergenic and repeat transcription in human, chimpanzee and macaque brains measured by RNA-Seq PLoS Comput Biol 2 010, 6:e1000843 Page of doi :10. 1186/gb-2 010- 11-7-125 Cite this article ... state than those of humans The study of Cavelier and colleagues [7] clearly illustrates that careful characterization of human and non-human primate transcriptomes can uncover large numbers of ... previous studies of the human and chimpanzee brain transcriptomes carried out using tiling arrays [8], high-throughput sequencing of expressed tags [9] and high-throughput sequencing of the complete...
- 3
- 229
- 0
Báo cáo y học: "Storage and allogeneic transplantation of peripheral nerve using a green tea polyphenol solution in a canine model'''' ppsx
... between the proximal and distal nerve stumps of the ulnar nerve using 10- 0 epineural sutures in the Nakayama et al Journal of Brachial Plexus and Peripheral Nerve Injury 2 010, 5:17 http://www.jbppni.com/content/5/1/17 ... μg of genomic DNA, 0.5 U Taq DNA polymerase (AmpliTaq Gold, PerkinElmer Cetus, Shelton, CT), pM of each oligonucleotide primer, μl of a 2.5 mM solution of each dNTP, 2.5 μl of 10 PCR buffer, and ... of the entire neural area of each specimen The number of myelinated axons, the neural area, and the shortest diameter of each myelinated axon were calculated for each field The total number of...
- 8
- 400
- 0
báo cáo khoa học: "Cellular transfer and AFM imaging of cancer cells using Bioimprint" pdf
... depressions having opening widths of approximately 600 nm and 100 nm deep and formed as concave submersions Page of 10 (page number not for citation purposes) Journal of Nanobiotechnology 2006, 4:1 ... reflects the profile of the imaging tip rather than of the rupture Further illustrating the range and variation of cell morphologies, and the potential effect the thick layer of polymer has in ... nature and response of cells, yielding insights into drug responses and effects Considerable variability in the sizes of dimple depressions and ruptures, as well as dynamic formation and grouping of...
- 10
- 263
- 0
Geochemical and Hydrological Reactivity of Heavy Metals in Soils - Chapter 10 ppsx
... absorption bands at 1572 and 1603 cm−1 for DOM from pig manure and green manure, respectively, together with the relative strong bands at 105 0 and 1 110 cm−1 suggested that DOM of green manure and pig ... behavior of DOM derived from organic wastes in soils and its effect on heavy metal mobility, and to propose areas of future research 10. 2 FRACTIONATION AND CHARACTERIZATION OF DOM The physical and ... hydrophilic fractions (Maxin and Kögel-Knabner, 1995; Totsche et al., 1997) Gel permeation chromatography by using Sephadex G-15, G-75, or G -100 and ultrafiltration by using a range of polymer-based membrane...
- 25
- 479
- 0
Báo cáo sinh học: "Distinguishing between hot-spots and melting-pots of genetic diversity using haplotype connectivity" pot
... Serrão E, Duarte CM: Spectrum of genetic diversity and networks of clonal organisms J R Soc Interface 2007, 4 :109 3- 1102 13 Excoffier L: Patterns of DNA sequence diversity and genetic structure after ... every pair of vertices is linked by an edge, which we denote by G * (Y) For every spanning tree T of G * (Y), that is, a subgraph of G*(Y) that is a tree and that contains all vertices of G*(Y), ... length of the restriction of the phylogeny to Y (i.e the length of the shortest subtree spanned by the elements in Y) which we denote by PD(Y) This measure has been used in the analysis of intraspecific...
- 10
- 281
- 0
Synthesis and molecular assemblies of d10 metal complexes bearing 9, 10 disubstituted anthracene ligand
... the ground and excited states of the molecule Such a similarity is also observed for the two crystal forms of complex The profiles of the 39 solid state emission spectra of complexes and are similar ... with occupancies of 0.5 and 0.5 The 24 (a) (b) (c) Figure 2.12 (a) ORTEP diagram of a monomer of 4·0.5Et2O (the disorder of one of the nitrate groups is shown by bonds of open lines and the dashed-open ... stretching signal of C C around 2100 cm-1 2.2.2 Crystal structures ( -PAnP)(AuCl)2 (1) Two crystal forms of 1·0.5Et2O18b and 1·CH2Cl2 were obtained by slow diffusion of Et2O and n-hexane into...
- 246
- 193
- 0
Condition monitoring and fault diagnosis of induction machine using artificial intelligence methods and empirical mode decomposition
... 20Hz 103 Figure 6.9: IMF10 and IMF11 (residue) of the machine signatures at 30Hz 103 Figure 6 .10: IMF10 and IMF11 (residue) of the machine signatures at 40Hz 103 Figure 6.11: IMF5-9 of the ... 103 6.5.5 Discussions: Significance of IMF5, IMF6, IMF7, IMF8, IMF9 104 6.6 Visualization of the Comparison results by SOM 107 6.7 Discussions: Discovery of Unique Features by SOM ... the topographical properties of the input space [105 , 106 ] It was invented by Professor Teuvo Kohonen, Helsinki University of Technology, Finland, during the 1960s, and it is sometimes called a...
- 180
- 655
- 0
Condition monitoring and fault diagnosis of induction machine using artificial intelligence methods and empirical mode decomposition
... 20Hz 103 Figure 6.9: IMF10 and IMF11 (residue) of the machine signatures at 30Hz 103 Figure 6 .10: IMF10 and IMF11 (residue) of the machine signatures at 40Hz 103 Figure 6.11: IMF5-9 of the ... 103 6.5.5 Discussions: Significance of IMF5, IMF6, IMF7, IMF8, IMF9 104 6.6 Visualization of the Comparison results by SOM 107 6.7 Discussions: Discovery of Unique Features by SOM ... the topographical properties of the input space [105 , 106 ] It was invented by Professor Teuvo Kohonen, Helsinki University of Technology, Finland, during the 1960s, and it is sometimes called a...
- 180
- 523
- 0
CHEMICAL AND OPTICAL PROPERTIES OF MOLECULAR COMPLEXES USING MATRIX ISOLATION SPECTROSCOPY
... Ar Ar Ar Ar Ar Ar Ar N2 ^3, cm 103 9.7 104 3.0 103 9.9 104 2.0 104 0.9 104 1.4 103 7.2 104 0.2 104 7.2 103 5.5 103 3.7 103 2.1 103 2.0 104 9.1 104 7.6 103 0.0 102 8.0 102 6.0 101 9.5 102 3.0 Ai^3, cm 0.2 1.0 1.2 1.7 ... (1983) 108 A Engdahl and B Nelander, J.Chem Phys 86, 4831 (1987) 109 A Engdahl and B Nelander, Chem Phys 100 , 273 (1985) 110 T L Tso and E K C Lee, J Phys Chem 89, 1612 (1985) 111 A Engdahl and ... of interaction depends on the difference in energy of the two concerned orbitals The concept of hard and soft acids and bases (HSAB principle) first developped by Pearson 33 then by Douglas and...
- 44
- 436
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose