... erythrocytes in the extent of binding with thelectins concanavalin A, Lens culinaris agglutinin(LCA), and Ricinus communis agglutinin (RCA)(Fig. 3), ruled out the blood origin and supported therenal ... added before heating for 2 min at 42 °C.Then, 200 U of Moloney murine leukaemia virus reversetranscriptase was added, and synthesis of cDNAs was per-formed for 50 min at 42 °C in a volume of 20 ... butyrylcholinesterase activityassayed in kidney. This proposal is in agreementwith the cytochemical staining of acetylcholinesterase in the capsule of Bowman [41] andof acetylcholines-terase and...
... vitamin E, vitamin A, vitamin C, ascorbic acid, Se, β-carotenoids, copper and zinc.8–10 Vitamin E is a fat soluble vitamin and is well-known forits cellular antioxidant and lipid lowering ... distribution and nitrogen adsorption-desorption isotherm of the samples were obtained using a gas sorption analyzer (NOVA 1000). Each of the AC and MAC samples was heated by passing N2 gas for 45 min ... (NAA),26 and the integrity of the sample is not changed in any manner by prechemistry or the addition of any foreign materials before irradiation, thus the problem of reagent-introduced contaminants...
... levelincreased in OccWT-overexpressing cells, and that2-APB inhibited the effects of OccWT. Therefore,the increase in intracellular calcium may explain theinduction of apoptosis and reduction ... additionaloccludin variant deleted in exon 9 (OccDE9). On thebasis of a comparative analysis of the involvement of wild-type occludin (OccWT) and variant occludin in apoptosis and invasion, as determined ... roles in the induction of mitochondria-mediated apoptosis and the inhibition of invasion, along withthe downregulation of matrix metalloproteinase expression. In addition, byusing the calcium indicator...
... 2001. Xiandai Hanyu Yufa Yanjiu. Beijing: Com-mercial Press.118 Categorial Fluidity in Chinese andits Implications for Part -of- speech TaggingOiYeeKwongBenjamin K. TsouLanguage Information ... tagged corpus for use in lexicography and other natural language processing tasks. Focuswill also be on a more detailed synchronous and longitudinal study of the verb-noun and other categorial ... captured in thetagging of large corpora to provide an importantresource for the study of this special linguisticphenomenon, as well as for lexicography and natural language processing. In Section...
... Poland´{AbstractDue to the presence andsignificanceof alumina in the natural aquatic environment andits increasing application in drinking and wastewater purification, the knowledge of ... attraction, for- mation of a coordinate link with a single donor group and formation of a chelate complex. This variety of HS-alumina bonds formation results from HS complexstructure and randomness of ... structure of alumina andits possible interactions with organic and inorganiccompounds in water are of great importance. This is of particular importance in both the understanding of natural...
... November2006)doi:10.1111/j.1742-4658.2006.05596.xThe zinc-finger cellular nucleic acid binding protein (CNBP) is a strikinglyconserved single-stranded nucleic acid binding protein essential for normalforebrain formation during mouse and chick ... hpfembryos were incubated during 15 min with different kinaseinhibitors at 30 °C and then used to analyse in vitro recom-binant CNBP phosphorylation in kinase buffer containing100 lm ATP and 5 lCi ... concentration of 2nm, and accuratemolar amounts of fusion proteins were added as indicated.Final reaction volume was 20 lL. Binding reactions wereincubated for 30 min at 37 °C and then loaded...
... c.secombes@abdn.ac.ukAbbreviations: IL, interleukin; IL-1Ra, interleukin 1 receptorantagonist; ICE: interleukin 1b converting enzyme; IL)18 BP,interleukin 18 binding protein; IFN, interferon; c-IP10, interferongamma induced ... Cloning and expression of interleukin-18binding protein. FEBS Lett. 445, 338–342.32. Novick, D., Kim, S.H., Fantuzzi, G., Reznikov, L.L., Dinarello,C.A. & Rubinstein, M. (1999) Interleukin-18 ... sequence of theIL-18 gene using primers F1 and RR1 and the PCRproducts sequenced. The PCR cycling programs for genomic PCR were 1 cycle of 94 °C for 3 min; 30 cycles of 94 °Cfor20s,60°C for 20...
... Kuhne,Pierre Flandin, Jean-Paul Giacobino and Patrick MuzzinDepartment of Medical Biochemistry, Faculty of Medicine, University of Geneva, SwitzerlandUncoupling protein-3 (UCP3) is a mitochondrial inner-membrane ... protein levels in SS and IMF mitochondria of gastrocnemius muscle in 24 h-fastedmice. Figure 5 illustrates the effects of 24-h fasting on theexpression of muscle UCP3 and COX per mg of protein in both ... expressionWe and others have shown that fasting induces upregula-tion of UCP3 mRNA expression in skeletal muscle of mice and rats (for review see [5]). To study further the regulation of UCP3 in fasting,...
... further examined with the relevant economic theories which are introduced in the Chapter 2 of the thesis. By applying economic theories, the roles and mechanisms of international agreements in conservation ... the path of balancing between industrialization and environmental degradation. Japan suffered quite heavily from pollutions during the fast industrialization process during 60‟s and 70‟s. ... envisage to contributing to conservation of the global environmental assets that are diminishing rapidly at an increasing rate. It is very timely and important in three main reasons. First,...
... levels of survivin give new insights in numerous alterations of theapoptosis machinery during the course of RA.We observed that survivin levels were clearly increased in synovial fluid and plasma ... variable and with RF, duration of RA, gen-der, and the survivin level as independent variables. Afteradjusting for the presence of RF, gender, and the duration of RA, a high level of survivin was ... course of RAAn ELISA was used for the evaluation of antibodies againstsurvivin of IgG and IgM isotypes in plasma andin synovialfluid of 129 patients with RA andof 34 healthy controls.The absorbance...
... an ideal candidate for linking inflammation and bone loss. Indeed, the therapeuticblockade of TNF is highly effective in preventing and/ or reduc-ing skeletal damage in conditions of chronic ... paraformaldehyde for 10 minutes at roomtemperature, rinsing with 0.1% Tween in PBS and digestingwith proteinase K (1 àg/ml; Roche, Mannheim, Germany).After rinsing again with 0.1% Tween in ... processin experimental arthritis [15].The relation of the metabolic activities of osteoclasts, evokingbone erosion, andof osteoblasts, mediating repair, is crucial for understanding the process...
... themethodology of looking at all treatment terminations and thenat only those terminations for the reason of ineffectiveness and terminated for the reason of toxicity. This was done bycensoring patients ... adjudication of the reason for withdrawal. In summary, the study by Saad and colleagues gives usimportant insights into daily-life use of TNF inhibitors in PsA, adisease that too often is secondary ... treatment terminations other than thereason of interest in the respective subanalysis. For example,if survival of drug effectiveness is analysed, a patient withtoxicity-related termination of therapy...
... observations and our tests of tentative hybridization and reinforce our hypothesis of thedistinction of three subspecies, I. c. bocqueti, I. c. mediterranea and I. c. chelipes of ... similarity of interbreedings between and within I. chelipes subspecies. Negative results and unsuccessful matings wereobserved when I. b. basteri was involved. According to ... estimate the intraspecific and the interspecificgenetic divergence.2. MATERIALS AND METHODSLaboratory cross-breedings were tested: intrasubspecific, intersubspecific and interspecific...
... the programme and the philosophy o fSDM by s eminars, outreach visits, and a brochureexplaining details of the programme.Recruitment and samplingWe invited a convenience sample of 91 primary ... one-half of patients want to be involved in decision making,but a far smaller percentage of them are actuallyinvolved. The willingness of physicians to involve theirpatients in decision making ... for multiple testing. This has to beconsidered when interpreting the results [28].Figure 2 Individual risk information with smileys. Within the module regarding oral antidiabetics (metformin),...
... narrowing down of the pool of possible victim matches in the forensic investigation processand help in establishing identification of the individual. Stature can be estimated from skeletal remains ... be used in estimation of stature in forensic and legal examinations. Stature estimation is commonly reported in forensic case work pertaining to adult populations and less commonly in sub-adult ... in a sub-adult population and hence the findings of the study cannot be compared per se. In such a forensic investigation, inter-observer and intra-observer error play an important role in...