preparation of vero cells
Báo cáo hóa học: " The synergistic effect of IFN-α and IFN-γ against HSV-2 replication in Vero cells is not interfered by the plant antiviral 1-cinnamoyl-3, 11-dihydroxymeliacarpin" pptx
... Enumeration of viable cells Vero cells were established at a density of × 104 cells/ well in 96-well plates and 12 h later fresh culture medium or medium containing 50 μg/ml of CDM or 100 IU/ml of IFN-α ... presence of CDM (50 μg/ml) The number of surviving cells was determined at 12, 24, 36 and 48 h p.i (Figure 2c) A comparison of Figure 2b and 2c shows that in the presence of CDM alone Vero cells ... assays, Vero cells were seeded in 24well plates at a density of 105 cells per well, and 12 h later cultures were treated with vehicle, 100 IU/ml of IFN-α and/or 100 IU/ml of IFN-γ After 16 h of IFN...
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báo cáo hóa học:" Preparation of TiO2 nanotube/nanoparticle composite particles and their applications in dye-sensitized solar cells" doc
... nanotubes [TNTs] The preparation of TNTs by a hydrothermal treatment of TiO2 powder in a 10-M NaOH aqueous solution has been reported [8-9] The use of oxide semiconductors in the form of nanorods, nanowires, ... level of dye adsorption on TiO2 in the form of nanorods and nanotubes is expected because of the high surface area of these nanostructures It is expected that the photoelectrical performance of ... curves of DSSCs based on the pure TiO2 cell, 10, 20, 30, 50, and 100 wt.% TNT cells, and cells without any TNT under AM 1.5 illumination (100 mW/cm2).One of the most important parameters of a solar...
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Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
... successful coupling of the RGD to the surface of the liposomes Assay of particle sizes and morphological observation of RGD-M-LCL The average size of liposomes processed by the microfluidizer was ... of matrine in cancer cells RGD-M-LCL significantly inhibited the growth of Bcap-37, HT-29 and A375 cells, compared with cells treated with the free matrine (P
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Tài liệu Experiment 17 Preparation of Methyl Orange ppt
... excess of a reagent that could decompose or cause decomposition and produce tar to contaminate our dye, you need to weigh the quantities of solid reagent very carefully to the accuracy of 0.05 ... calculate for yourself some of the amounts of needed reagents After you have calculated them, confirm your results with the instructor before proceeding Dissolve 0.010 mole of sulfanilic acid (anhydride) ... Dissolve 0.010 mole of sulfanilic acid (anhydride) in about 50 ml of a solution of sodium carbonate containing 0.010 to 0.0125 moles of sodium carbonate in a 125 ml Erlenmeyer flask The solution...
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Tài liệu EXPERIENCE OF THE INSTITUTE OF ENERGY IN THE PREPARATION OF THE RENEWABLE MASTER PLAN docx
... has been prepared by Institute of Energy Institute of Energy Main Contents of RE Master Plan and Some Preliminary results 2.1 Organization of RE MP Present status of RE development 1.1 Existing ... 10 million tons of oil) Types of biomass can be exploited at the industrial scale: rice husks; leaves, top of sugar canes & baggage; coffee husks and wood residues Institute of Energy Biogas ... assessment on potential RE resources, cost, and benefits of environment Lack of manpower Institute of Energy THANK YOU FOR YOUR ATTENTION! Institute of Energy ...
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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx
... (CLP), the hepatic mRNA level of CD163 was similar with that of the sham group, which markedly decreased to approximately 7.0% of that of the CLP group (Fig 1) Table Effect of GdCl3 on serum aminotransferase ... to the inhibition of CYP activity observed in the absence of changes in protein expression in rabbit hepatocytes [22] ROS indirectly reduced the activity of selected isoforms of CYP by inducing ... role of KCs in the regulation of CYP isoform activities and gene expression profiles, partly by investigating the inflammatory signaling pathway Sham CLP GdCl GdCl3 + CLP CD163 β-actin Fig Effect of...
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Tài liệu The Electrical Properties of Cancer Cells pptx
... normal cells proliferate and either replace the destroyed and damaged cells with new cells or scar tissue One characteristic feature of both proliferating cells and cancer cells is that these cells ... charge carriers and electrical properties of cells • The cells of the body are composed of matter Matter itself is composed of atoms, which are mixtures of negatively charged electrons, positively ... properties of cancer cells part • Some of the characteristic features of cancerous cells that affect their electrical activity are: Cancer cells are less efficient in their production of cellular...
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Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx
... stimulation of ERK1 ⁄ and Pak1 pathways, gefitinib might lead to inhibition of invasiveness of human cancer cells through the inhibition of ERK1 ⁄ and Pak1 The use of gefitinib in cells with activated ... phosphorylation state of their proteins, thereby changing the amount and localization of Bcl-2 family members However, each type of cancer has its own way of disrupting the balance of the networks of signaling ... inhibition of Bax phosphorylation Regulation of Bax also occurs by mechanisms other than phosphorylation Gefitinib inhibits growth of human gallbladder adenocarcinoma cells (HAG-1) by arresting the cells...
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Tài liệu Báo cáo khoa học: Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells doc
... orientation of the primers Bold font represents the sequence of mature miR9* and italic fonts the sequence of mature miR9 (C) RT-PCR analysis of pri-miR9s during the neuronal differentiation of P19 cells ... constructions of reporter gene to monitor primary and mature form of miR9 To detect the transcript level of pri-miR9, the upstream region of pri-miR9-1 was isolated from the genomic DNA of HeLa cells ... for for for for for for expression of pri-miR9-1 expression of pri-miR9-1 expression of pri-miR9-2 expression of pri-miR9-2 expression of pri-miR9-3 expression of pri-miR9-3 neuronal differentiation...
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Tài liệu Báo cáo Y học: Differential response of neuronal cells to a fusion protein of ciliary neurotrophic factor/soluble CNTF-receptor and leukemia inhibitory factor pot
... Neuronal differentiation of PC12 cells by Hyper-CNTF (A) Morphology of PC12 cells grown for 48 h in serum-containing medium in the absence of factors (medium) or in the presence of 100 ngÆmL)1 CNTF, ... consequences such as stimulation of proliferation, stimulation of differentiation, prevention of differentiation, prevention of apoptosis and activation of a family of genes coding for the acute ... Hyper-IL-11 constructs First, the spectrum of target cells is more restricted All cells in the body express gp130, whereas only some cells including most cells of the nervous system express the LIF-R...
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Therapeutic ultrasound as a potential male contraceptive: power, frequency and temperature required to deplete rat testes of meiotic cells and epididymides of sperm determined using a commercially available system doc
... al [3] In a series of publications, it was shown that a single application of ultrasound could result in a dramatic loss of germ cells from testes and that this loss of germ cells was reversible ... bottom of the ring to maintain a minimum distance of cm between the bottom of the ultrasound chamber and the proximal portion of the scrotum Determining the true effective radiating area (ERA) of ... percentage of counts that fell within each range For example, if 75% of a group’s sperm counts fell in the second range of 11-20 × 10 and the remaining 25% of the counts fell in the fourth range of 41-80...
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Báo cáo khoa học: Isoquinoline-1,3,4-trione and its derivatives attenuate b-amyloid-induced apoptosis of neuronal cells pdf
... studies have suggested that inhibition of caspase-3 activity can block induction of apoptosis by Ab in primary neuronal cells and PC12 cells [14] The neurotoxicity of Ab seems to depend on its ability ... evaluated from the ability of cell cultures to reduce MTT, an indication of metabolic activity The assay is based on the ability of the mitochondrial dehydrogenase enzyme of viable cells to cleave the ... using PC12 cells Using a phase-contrast microscope, we observed significant morphological changes of PC12 cells treated with Ab(25–35) and caspase-3 inhibitors after 48 h (Fig 3A) In cells treated...
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Báo cáo khoa học: Factors affecting habituation of PC12 cells to ATP potx
... determine the effect of a test condition on the response of PC12 cells to a stimulant, the first response of cells under test conditions was divided by the response of the cells to an identical ... response of PC12 cells to 300 lM ATP under control conditions The scaled response of PC12 cells to ATP and 2MeSATP in the various conditions studied are shown in Fig Habituation of the cells to ... solution similar to that of 30 lM ATP at the same [Mg2+] Figure (bars 6–8) shows the effect of altering the [Mg2+] on the response of PC12 cells to 60 lM 2MeSATP The response of the cells in a 0.0-mM...
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Preparation of dairy products potx
... top of the milk and form a layer of cream Buffalo milk also forms some cream on top, but other kinds of milk, such as that of sheep and goats, hardly form a layer of fat at all For these types of ... making cheese The quality of milk used to make cheese Coagulation of the milk Separating curd and whey The use of cheese whey Collection and preservation of the curd Maturation of the cheese Cheese ... 5.5 Starter cultures The development of lactic acid bacteria Cultivation of starter cultures of lactic acid bacteria Growth of starter cultures Maintenance of starter cultures Preparing your own...
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Mechanical preparation of root canals: shaping goals, techniques and means potx
... ProFile 0.04 & 0.06 ProFile 0.04 ProFile 0.06 Ni–Ti system K3 Deflection angle Frequency of use Type of Ni–Ti instrument Preflaring torque Cleaning procedures ProFile ProTaper K3, ProTaper & ProFile ... Non-Instrumental Technique of Lussi demonstrating insufficient cleaning ability with lots of remaining debris and tissue (courtesy of Prof T Attin, ¨ Gottingen and Prof A Lussi, Bern) Preparation of oval root ... canal preparation include manual preparation, automated root canal preparation, sonic and ultrasonic preparation, use of laser systems, and NITs Ingle (21) described the first formal root canal preparation...
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Báo cáo " Preparation of nano-structural MnO2 in ethanol-water media coated on calcinated laterite and study of its arsenic adsorption capacity " docx
... working solutions of Mn(II) are series of 0, 5, 10, , 100 % of ethanol in 3.10-2 MnSO4 solution Similarly, working solutions of Mn(VII) include series of 0, 5, 10, , 100 % of ethanol in 2.10-2 ... solution The procedure of MnO2 nanoparticles formation was followed: slowly add series of KMnO4 solutions one by one into the series of MnSO4 solutions The dropped rate of mixed reagent was 2.5 ... dried denaturated laterite with size of 0.5 – 1.0 mm diameter and dropped into colloidal solution of MnO2 Then softly shook the mixture in 60 When almost of MnO2 particles adsorbed on the laterite...
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Báo cáo khoa học: Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin pptx
... induced apoptosis of human tumor epithelial cells [21] Accumulation of nCLU correlated with inhibition of cell proliferation and induction of apoptosis of human tumor epithelial cells caused by ... that c-fos-induced apoptosis of HaCaT cells was Bax-mediated and involved downregulation of sCLU and upregulation of nCLU Differential effects of constitutive expression of sCLU and Bcl-2 on vanadium-induced ... treatment of HaCaT NeoT and HaCaT sCLU cells with increasing concentrations of VOSO4 resulted in the induction of DNA fragmentation at concentrations of VOSO4 as low as 25 lm, enforced expression of...
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Báo cáo khoa học: A novel serine protease highly expressed in the pancreas is expressed in various kinds of cancer cells potx
... al A novel serine protease in various cancer cells A B C Fig Secretion of prosemin protein and expression of prosemin mRNA in various kinds of cancer cells (A) Conditioned medium (25 lg) from various ... various kinds of cancer cell lines and in clinical samples of ovarian carcinomas The characterization and functions of prosemin are described Results cDNA cloning and structure of the prosemin ... protease in various cancer cells 4913 A novel serine protease in various cancer cells identity, respectively (Fig 2) Amino acid alignment of these proteases shows that 10 of the 12 cysteine residues...
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Báo cáo khoa học: Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells pot
... effect of HePC on HepG2 cells was determined by the MTT test, based upon the conversion by viable cells of a tetrazolium salt into a blue formazan product [19] The toxicity of HePC against HepG2 cells ... rate of incorporation of CDP-[methyl-14C] choline into PC, according to the method reported by Cornell [24], using at least 50 lg of membrane protein Fig Cytotoxic effect of HePC on HepG2 cells Cells ... biosynthesis of PC in HepG2 cells at the level of CT activity It interferes with the translocation process of CT from the cytosol to the membrane and finally leads to an inhibition of the biosynthesis of...
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Báo cáo Y học: Growth inhibition of mammalian cells by eosinophil cationic protein pptx
... 10 lM Effect of ECP on A431 and K562 cells At lM ECP, the growth of K562 cells was completely suppressed whereas growth inhibition of A431 cells was % 50% (Fig 2) As ECP is a member of the supergene ... demonstrate that ECP impairs the growth of cells Cellular localization of ECP Fig Growth-inhibitory eect of ECP on K562 cells (A) Cells were seeded at 25 000 cells per 35-mm dish Simultaneously, ... was shown after days of treatment with ECP, which was Fig Suppression of cell growth in the presence of ECP Left, the percentages of viable cells under various concentrations of RNase A (s), poly...
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