preparation of mrna morpholino and needles
Genetic approaches to study liver development in zebrafish core component sec13 in COPII complex is essential for liver development in zebrafish
... formation of the rostral diverticulum of the gut, remodeling of the extracellular matrix around the hepatoblasts and of E-cadherin-based connections between the cells, and proliferation and migration ... for genetic screening 57 2.15 Microinjection 57 2.15.1 Preparation of mRNA, morpholino, and needles 57 2.15.2 Microinjection 58 2.16 Alkaline phosphatase ... initiates a series of slow and progressive changes, such as the arising of germ layers, proliferation of cell linages and subsequent formation of different tissues and organs This process does...
- 172
- 397
- 0
Báo cáo khoa học: The propagation of hamster-adapted scrapie PrPSc can be enhanced by reduced pyridine nucleotide in vitro pdf
... absence of NADPH After seven rounds of PMCA, clear PrPSc signals were observed at ScBH dilutions of 10)4 to 10)10, and even at a 10)12-fold dilution a weak band was observed in the presence of NADPH ... NADPH and the other added to an equal volume of NaCl ⁄ Pi After 24 cycles PMCA as the first round, 10 lL of product was mixed with 90 lL of fresh NBH for the next round of PMCA The five left-hand ... vitro Fig Influence of other analogues of NADPH and ascorbic acid on PrPSc propagation in PMCA ScBH of SHa-263K were mixed with NBH at : 100 dilutions by the addition of 10 lM of NADPH, NADP, NADH,...
- 10
- 342
- 0
Báo cáo khoa học: Tyrosine-dependent basolateral targeting of human connexin43–eYFP in Madin–Darby canine kidney cells can be disrupted by the oculodentodigital dysplasia mutation L90V ppt
... loss of tooth enamel, and abnormal soft tissue separation of two or three digits [24,25] Depending on the particular Cx43 mutation, a wide range of other abnormalities, including neurological and ... Tiwari-Woodruff PhD (Department of Neurology, UCLA School of Medicine) for the use of her microscope, Guido Faas PhD (Department of Neurology, UCLA School of Medicine) for performing all of the transepithelial ... observed [2637] As yet, there is no clear understanding of the relationship between genotype and phenotype, although functional evaluations of most of the mutations that have been studied have demonstrated...
- 14
- 433
- 0
Báo cáo y học: "Radiographic joint damage in rheumatoid arthritis is associated with differences in cartilage turnover and can be predicted by serum biomarkers: an evaluation from 1 to 4 years after diagnosis" pot
... score is the sum of the joint space narrowing and the erosion scores of both hands and feet, ranging between and 448 (a higher score indicating more damage) Because combinations of markers measuring ... (n = 20) and interday (n = 200) coefficients of variance for each biomarker were, respectively: for C2C, 10– 17% and 14%; for C1,2C, 5–14% and 13%; for CS846epitope, 4–12% and 12%; and for CPII, ... the process of bone and cartilage damage, which may therefore be predictive of radiographically assessed progression of joint damage As a result of cartilage damage, key components of the extracellular...
- 9
- 525
- 0
Báo cáo y học: "Granulocyte-CSF induced inflammation-associated cardiac thrombosis in iron loading mouse heart and can be attenuated by statin therapy" pptx
... infiltration and inflammatory profile between them As expected, I +G hearts had higher levels of 4-HNE and 8-OHdG (both are index of oxidative stress), and increased expression of CD45 (leukocyte ... end of the second and fourth week Similar protocols of iron loading and G-CSF supplement to mice were previously described [3,20] Simvastatin or tirofiban treatment to I+G mice, blood counts and ... group, and I+G plus statin (I+G+St) and the control group was collected at the end of 4th week and compared Incidence of thrombi formation were 0/10 in the control group, 7/10 in the I+G, and 2/10...
- 15
- 342
- 0
Strategic environmental assessments may be used to compare different energy scenarios, and a more sustainable power plan can be developed by incorporating the wider impacts considered during the assessment process
... in demand under the energy efficiency scenario allows a reduction of 16 GW of lignite and coal capacity under the global impacts case and of GW of large hydropower capacity (22 plants), GW of nuclear ... generation to obtain estimates of the total costs of alternative PDPs.15 These costs represent the costs of health impacts of pollutants, the loss of productive land and of fisheries, the need to ... Cambodia and the Lao PDR are weighted averages of those for Thailand and Viet Nam The additional renewable energy capacity in the renewable energy scenario allows the displacement of GW of lignite and...
- 50
- 456
- 0
PERFECT PRESENTATIONS Presenting with impact is a skill that can be learned by anyone
... dozens of really boring slides read off by the presenter Design of the presentation • Crucial • Words (text) • Vocal Input • Visual 7% 38% 55% Presenter • Must deliver the mesage clearly and with ... Effectively with interest and enthusiasm, • in a way that keeps the attention of your audience Presentation • Always about selling something • An idea • A different way of thinking Presentation ... application and good training anyone can be a fluent and confident speaker • A great asset you will use throughout your career Presentation • • • • To get across the message, To inform, To explain, and...
- 23
- 393
- 0
Báo cáo khoa học: "From Extractive to Abstractive Meeting Summaries: Can It Be Done by Sentence Compression?" pdf
... final FPs and about a hundred simplification rules Examples of the final FPs are: ‘you know’, and I think’, ‘some of , ‘I mean’, ‘so far’, ‘it seems like’, ‘more or less’, of course’, ‘sort of , ‘so ... “for example”, of course”, and “sort of Removing these fillers barely causes any information loss We propose to use web information to automatically generate a list of filler phrases and filter them ... S Renals, and J Carletta 2005a Extractive summarization of meeting recordings In Proc of INTERSPEECH G Murray, S Renals, J Carletta, and J Moore 2005b Evaluating automatic summaries of meeting...
- 4
- 342
- 0
Báo cáo y học: "Chylopericardium after cardiac surgery can be treated successfully by oral dietary manipulation: a case report" docx
... However on application of low pressure (10 cm of water) suction on day nine, a small piece of debri was dislodged from the drain and there was a sudden drainage of 450 ml of chyle Therefore thrice ... production of lymph and allow the spontaneous closure of the fistula in the majority of cases This option is also more palatable for the patient and avoids the potential complications of total ... the method of choice [6,9,11], we advocate one of minimal intervention with a dual strategy of decreasing lymph production and ensuring adequate protein intake to counter any effects of the potential...
- 3
- 426
- 0
Thuyết trình tài chính quốc tế CAN CENTRAL BANKS’ MONETARY POLICY BE DESCRIBED BY A LINEAR (AUGMENTED) TAYLOR RULE OR BY A NONLINEAR RULE
... Surico (2007) xem xét vai tròn cung tiền ECB Cecchetti cộng (2000), Borio Lowe (2002), Goodhart Hofmann (2002),… đưa chứng cho ngân hàng trung ương nên quan tâm đến giá tài sản Montagnoli Napolitano...
- 63
- 928
- 13
Thuyết trình tài chính quốc tế Can central bank’s monetary policy be described by a linear (augmented) Taylor rule or by a monetary rule
... current values of these variables Fourc¸ ans and Vranceanu (2004) and Sauer and Sturm (2007) also stress the importance of considering a forward-looking Taylor rule in the analysis of the ECB’s ... BRIEF REVIEW OF LITERATURE The role of asset prices is an important issue considered in some studies Cecchetti et al (2000), Borio and Lowe (2002), Goodhart and Hofmann (2002), Sack and Rigobon ... G(η, c, st ) is continous and bounded between zero and one, the combined parameters, ζ, will fluctuate between and ψ and ψ+ω and change monotonically as a function of st COMPANY LOGO www.themegallery.com...
- 59
- 1,096
- 0
Thuyết trình Can central banks’ monetary policy be described by a linear (augmented) Taylor
... Bank of England - Bank of Japan Libik and Schorfheide (2007) found a similar result for - Central Banks of Canada - Central Banks of England LITERATURE REVIEW Extent by cosidering the effect of ... low power and poor performance of these tests in small samples, we report the results of two different unit root tests (Dickey and Fuller, 1979 and Ng and Perron, 2001) and the results of the KPSS ... Inflation and Output Gap ASTRACT RESULT OF PAPER The monetary behaviour of the European Central Bank (ECB) and Bank of England (EOB) is best descibed by a nonlinear rule The behaviour of the Federal...
- 90
- 626
- 1
Tiểu luận tài chính quốc tế Can central banks’ monetary policy be described by a linear (augmented) Taylor rule or by a nonlinear rule
... et al., 2005), nonlinear central bank preferences (Dolado et al., 2000; Nobay and Peel, 2003; Ruge-Murcia, 2003 and Surico, 2007a) or both (Surico, 2007b) Đặc biệt, Surico (2007b) nghiên cứu ... hay không nên nhắm mục tiêu đến loại biến Cecchetti et al (2000), Borio Lowe (2002), Goodhart Hofmann (2002), Sack Rigobon (2003), Chadha et al (2004) Rotondi Vaciago (2005) xem xét điều quan ... biến ngoại sinh thêm vào sai số Phương trình (5) ước lượng phương pháp GMM (generalized method of moments) Theo Clarida cộng (1998, 2000), phương pháp thích hợp với phân tích toán kinh tế nguyên...
- 26
- 500
- 1
Báo cáo y học: "The association of meat intake and the risk of type 2 diabetes may be modified by body weight"
... lians (50g/lian) per unit of time A lian is the unit of weight in China equivalent to 50 g The reproducibility and validity of the FFQ was assessed in a random sample of 200 participants who completed ... alcohol consumption and history of hypertension, coronary heart disease (CHD), stroke, and cancer Anthropometric measurements, including weight, height, and circumferences of the waist and hips, were ... part of a dietary pattern 155 associated with a higher risk of type DM [17] To our knowledge there are no other reports of a direct link between poultry consumption and risk of diabetes or of interactions...
- 8
- 701
- 0
EU ANTI-DUMPING LAWSUIT AGAINST VIETNAM - WHAT CAN BE LEARNT FROM THE FOOTWEAR CASE
... research, with data and information obtained from the Official Journals of the European Union, Action Aids Vietnam, the Vietnam Leather and Footwear Association, official and unofficial reports, ... value” of the product, which is calculated on the basis of the cost of production, plus selling, general, and administrative expenses, and profits Costs shall normally be calculated on the basis of ... production and opinions of the complaint Examining the complaint A complaint shall contain the following information: - Identity of the complainant and a description of the volume and value of the...
- 66
- 538
- 4
Without grammar very little can be conveyed, without vocabulary nothing can be conveyed
... participants and the findings of the study Chapter discusses the use of vocabulary learning strategies by the students of HV Gifted high school and the differences among groups of students by gender and ... system, realization of language as a means of communication and interaction, management of affective demands, and monitoring of L2 performance O’Malley and Charmot (1990) analyzed learning strategies ... this group of strategies, remembering parts of speech and learning the words of an idiom together were used with high frequency Up to 76.1% of the students employed the former and 59.7% of the total...
- 48
- 1,320
- 0
Without grammar very little can be conveyed, without vocabulary nothing can be conveyed
... participants and the findings of the study Chapter discusses the use of vocabulary learning strategies by the students of HV Gifted high school and the differences among groups of students by gender and ... system, realization of language as a means of communication and interaction, management of affective demands, and monitoring of L2 performance O’Malley and Charmot (1990) analyzed learning strategies ... employed and became automatized They are changeable Table 1: Components of learning strategies Source: Wenden and Rubin (1987: 7-8) Oxford (1990:9) offers a more comprehensive list of the features of...
- 35
- 1,830
- 1
EU ANTI-DUMPING LAWSUIT AGAINST VIETNAM - WHAT CAN BE LEARNT FROM THE FOOTWEAR CASE?
... research, with data and information obtained from the Official Journals of the European Union, Action Aids Vietnam, the Vietnam Leather and Footwear Association, official and unofficial reports, ... production and opinions of the complaint Examining the complaint A complaint shall contain the following information: - Identity of the complainant and a description of the volume and value of the ... description of the volume and value of Community production of the like product accounted for by such producers - A description of the allegedly dumped product, the names of the country or countries of...
- 84
- 544
- 0
Drilling Can Be Fun
... words Then point to a piece of paper and ask a student to tell you which word it is Do this with all the words Then screw all the pieces of paper into balls Take three and ask the students to tell ... in large letters on pieces of paper Draw a picture on the paper, too, to show the meaning Put all the pieces of paper in a line on the desk Hold one up, say the word and get the class to repeat ... throw the pieces of paper to each other, saying the word as they so For the second one you need pictures of the words you are going to drill Write the words in a list on the board and practise them...
- 3
- 304
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25