preparation of cells for the transmigration assay seeding endothelial cells on glass coverslips
Báo cáo y học: "Comparison of capillary based microflurometric assay for CD4+ T cell count estimation with dual platform Flow cytometr" pps
Ngày tải lên :
10/08/2014, 05:20
... highlights the need of development of laboratory based quality control check on the equipment Conclusion 100 0 samples Figure EasyCD4 assay performance of stabilized blood samples in the guava performance ... per second) The high event rate was found to occur in case of the samples having a high proportion of non-lymphocyte population in the sample So the run was considered valid if the variation between ... shows ± SD of the mean CD4 counts/mm3 (-) for that proficiency run The CD4 counts obtained by Guava EasyCD4 assay ( ) are between the limit of ± 2SD In conclusion, the availability of EasyCD4...
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báo cáo hóa học:" Regulatory activity of azabisphosphonate-capped dendrimers on human CD4+ T cell proliferation enhances ex-vivo expansion of NK cells from PBMCs for immunotherapy" potx
Ngày tải lên :
18/06/2014, 15:20
... capacity, C the concentration of the 3a-G1-Julo, Kd is the dissociation constant and k the coefficient of the non-specific fixation component Interestingly, competition experiments revealed that the ... different donors and then estimated the proportion of CD4+/FoxP3high cells vs NK cells in both conditions For all donors 3a-G1 prevented the generation of regulatory T cells and together with ... PBMCs In the absence of monocytes, the proliferation of purified CD4+ T cells is abrogated; therefore monocytes are required for the priming of autologous T cell proliferation Co-culturing monocytes...
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Tài liệu Báo cáo khoa học: TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses pdf
Ngày tải lên :
18/02/2014, 14:20
... represent the mean counts of triplicate wells, and the error bars represent standard deviations of triplicate well counts The concentrations on the x-axes are concentrations in the incubation reaction ... three-step assay: initial incubation of the sample and probe, addition and incubation of the sample and acceptor beads in the plate wells, and addition of donor beads with incubation (Fig 1B) The assay ... The units on the x-axes are concentrations in the acceptor bead preincubation reaction The signal level of untreated sample is indicated by the label ‘Heat shock’ on the graph (C) Replacing the...
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báo cáo hóa học:" Protective CD8+ T-cell responses to cytomegalovirus driven by rAAV/GFP/IE1 loading of dendritic cells" pdf
Ngày tải lên :
18/06/2014, 15:20
... a large proportion of the human population, and plays a significant role in the control of HCMV disease [49] The previous study led to the identification of several new classes of I MHC-restricted ... second reason could be the increased expression of CD80, CD86, and CD40 that may also contribute to generating the robust CTL response Conclusion In summary, our results demonstrate that the ... minutes before addition of the stimulated T -cells K562 cells were used as targets to observe natural killer (NK) cell activity In all of these CTL killing assays, spontaneous release of chromium...
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Báo cáo hóa học: " Characterization of the IFN-γ T-cell responses to immediate early antigens in humans with genital herpes" doc
Ngày tải lên :
20/06/2014, 01:20
... beads confirmed that the response was from CD4 cells Considering the efficiency of the magnetic bead separation used to prepare the samples, and the different assay conditions needed for the two ... CD8 cells and the free CD8 cells generate the same response under these assay conditions The ability of CD8 cells to respond to peptide when bound to beads suggests that antigen presenting cells ... that the dose dependency of the CD8 IFN-γ ELISPOT assay is related to the length of the peptides Although longer peptides may be less active at low doses, the concentration of peptide used for the...
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Báo cáo y học: "Collagen-induced arthritis in C57BL/6 mice is associated with a robust and sustained T-cell response to type II collagen" pot
Ngày tải lên :
09/08/2014, 10:21
... carried out most of the experiments, and contributed to the preparation of the manuscript GC carried out some experiments and contributed to the preparation of the manuscript MM performed some experiments ... mixed beyond weeks of age Page of (page number not for citation purposes) Each experiment was performed on a minimum of two occasions For macroscopic assessment of arthritis, the thickness of each ... detection antibody pairs (BD Biosciences) Drug therapy The therapeutic responses of arthritic B6 and DBA/1 mice to intraperitoneal administration of dexamethasone (0.5 mg/kg daily), anti-TNF monoclonal...
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Báo cáo khoa học: " Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo" ppsx
Ngày tải lên :
12/08/2014, 04:20
... numbers of pVP3 for real-time PCR reaction under the optimized conditions On the basis of the results of correlation coefficient (0.999) and PCR efficiency (98.7%), it was confirmed that the standard ... sensitive than the conventional PCR method; the former method can detect pVP3 copies down to dilutions of 2.8 × 101 copies/reaction and the latter one that can detect copies up to the dilutions of 2.8 ... final concentrations of each primer as 0.2 μmol/L and that of probe as 0.16 μmol/L The MgCl2 concentration was adjusted to 10 mM to obtain optimal FQ-PCR assay conditions Therefore, the optimized...
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Báo cáo y học: "Isoniazid prophylaxis differently modulates T-cell responses to RD1-epitopes in contacts recently exposed to Mycobacterium tuberculosis: a pilot study" pdf
Ngày tải lên :
12/08/2014, 15:20
... controversy is whether these immune assays can be used for monitoring the response to TB treatment Conflicting results are available on the effect of therapy for active TB disease on RD1 responses with ... bacilli may be the cause of the longer persistence of the T cell response over time and also be responsible for the generation and maintenance of a large population of memory cells, similar to ... among all these recent contacts nine did not respond at the first time of observation to RD1 selected peptides Five of them did not respond to QTF-G either, and therefore it is likely that these...
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Design of artificial microenvironment for cord blood CD34+ cells expansion ex vivo
Ngày tải lên :
04/10/2015, 15:44
... issue of the covalent strategy is the conservation of the desired bioactivity of the immobilized FN The degree of activity depends on the binding procedure and the substrate A combination of conjugation ... product for transplantation 2.2.1 Selection of candidate cells for expansion There are a number of issues related to the selection of a candidate stem population for ex vivo expansion Before determining ... free condition And as the CB cells are non adherent cells, the exchange of medium is not allowed during the expansion process, which will lead to the loss of cells and the increase the consumed...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
Ngày tải lên :
19/02/2014, 13:20
... in the peptide and the proximity of amide protons due to compact nature of the structure However, the coupling constant of most of the amide protons was in the range of 6–8 Hz, suggestive of ... of the calculated /-values from JHNa [28] The final structures which satisfy most of the NMR distance constraints were clustered together based on the rms deviation of the backbone atoms and the ... results in the formation of E-rosettes The ability of each of the designed CD2 peptides to inhibit CD2–CD58 interaction was evaluated by inhibition of E-rosette formation between Jurkat cells and...
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Báo cáo y học: " PDZ domain-binding motif of human T-cell leukemia virus type 1 Tax oncoprotein is essential for the interleukin 2 independent growth induction of a T-cell line" ppsx
Ngày tải lên :
13/08/2014, 09:21
... whether these two activities utilize the same mechanism Both the number and size of the transformed colonies of Rat-1/Tax1 cells were greater than those of Rat1/Tax2B cells, but the presence of ... essential for outgrowth of CTLL-2/Tax cells in the absence of IL-2 PBM is essential for outgrowth of CTLL-2/Tax cells in the absence of IL-2 (A, B) CTLL-2 cells (107) were transfected either with the ... growth, consistent with the absence of PBM in Tax2 Taken together with the strict conservation of PBM only in HTLV-1 Tax1 [33], these results suggest that HTLV-1 and Page of (page number not for citation...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
Ngày tải lên :
21/02/2014, 00:20
... phosphorylation state On the contrary, the modification of eEF1A mobility on bidimensional PAGE by increasing its pI value should be associated with the presence of other post-translational modifications ... Abbott, C.M (1998) The lethal mutation of the mouse wasted [wst] is a deletion that abolishes expression of a tissue-specific isoform of translation elongation factor 1alpha, encoded by the Eef1a2 gene ... showed that the labelled GT oligomer mainly recognized the more basic form of eEF1A, whereas a very weak interaction was found for the isoform of eEF1A focusing at a pH of 9.0 The recognition seemed...
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Báo cáo khoa học: Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycan Glycoengineering for enhanced cell binding and serum clearance of the neoglycoprotein pot
Ngày tải lên :
30/03/2014, 16:20
... to the preparation of the other N-glycan-bearing probes tested previously [12–14] Solid-phase assay The matrix for the assay was established by adsorption of neoglycoprotein to the surface of ... designation of spacer atoms illustrated for compound in Fig Preparation of neoglycoprotein A For conjugation of the derivatized dodecasaccharide to the carrier protein the amino group was transformed ... to the three tested types of sugar receptor in this assay system In contrast, the consideration of the data for the N-glycan with bisecting GlcNAc (B) with reductions in affinity underscores the...
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Báo cáo hóa học: " Strategy Escalation: An emerging paradigm for safe clinical development of T cell gene therapies Richard Paul Junghan" pdf
Ngày tải lên :
18/06/2014, 16:20
... cells respond to viral infections Further, the added costimulation renders designer T cells resistant to regulatory T cell suppression [14] The benefits of these modifications for improving therapy ... therapy Appendix 2: Future directions One may consider the structure of the × matrix for Strategy Escalation as deriving from inherent elements of T cell biology One dimension is how many T cells ... discussion that accounts for the molecular and biological features of the modified cells and the method of their proposed administration This Commentary presents an example of such a discussion from...
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Báo cáo hóa học: "Comparison of anti-CD3 and anti-CD28-coated beads with soluble anti-CD3 for expanding human T cells: Differing impact on CD8 T cell phenotype and responsiveness to restimulation" pot
Ngày tải lên :
18/06/2014, 16:20
... stimulation (Figure 5) T cell response to restimulation On occasion, the number of cells generated by one cycle of T cell expansion may be insufficient for the desired purpose, and further expansion ... To compare the impact of beads and anti-CD3, the expression of these markers on CD4 and CD8 cells was assessed before and after 2-3 weeks of in vitro stimulation The results from one representative ... and the number of viable cells detected per well by flow cytometry using the formula: The proportion of cells undergoing 0-6 divisions could then be quantitated based on the pattern of CFSE fluorescence...
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Báo cáo hóa học: "IL-2 as a therapeutic target for the restoration of Foxp3+ regulatory T cell function in organ-specific autoimmunity: implications in pathophysiology and translation to human disease" doc
Ngày tải lên :
18/06/2014, 16:20
... contexts of homeostasis and inflammation Therefore, the waning of Treg cells, and ensuing breakdown in the self tolerance, could depend on the in situ IL-2 environment These data all together suggest ... In the implications of such considerations lies the relevance of studies examining defects on a global population of Treg cells obtained from the peripheral blood, as opposed to examining the ... Modulation of the IL-2/IL-2R pathway for therapeutic purposes Given the strong link between IL-2 and autoimmunity, it seems appealing to consider the use of IL-2 as a Page of 12 therapeutic tool for...
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báo cáo hóa học: " Persistently elevated T cell interferon-γ responses after treatment for latent tuberculosis infection among health care workers in India: a preliminary report" pot
Ngày tải lên :
20/06/2014, 00:20
... they can be used for monitoring treatment responses [7,8,10] It has been hypothesized that short incubation assays (e.g both commercial assays use 16 – 24 hours incubation) detect responses of ... 26(Suppl 49):22S Wilkinson KA, Kon OM, Newton SM, Meintjes G, Davidson RN, Pasvol G, Wilkinson RJ: Effect of Treatment of Latent Tuberculosis Infection on the T Cell Response to Mycobacterium tuberculosis ... reduction of the antigen load by treatment decreases T cell responses [19,20] Although studies have examined the effect of active TB treatment on IFN-γ responses, the results have been inconsistent...
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Báo cáo sinh học: " Systematic identification of regulatory proteins critical for T -cell activation" doc
Ngày tải lên :
06/08/2014, 18:20
... Clone 4D9 was selected for low basal levels of CD69 expression and strong induction following TCR stimulation (see Additional data file with the online version of this article for details of the ... node and bone marrow (see Additional data file with the online version of this article for details of construction and assessment of the pTRA-cDNA libraries) After library infection, cells were ... desired cells were allowed to rest for days before another round of stimulation and sorting (c) Cells were split into two populations after the third round of sorting One half of the cells were...
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Báo cáo y học: " Chitosan Interferon-c Nanogene Therapy for Lung Disease: Modulation of T-Cell and Dendritic Cell Immune Responses" pps
Ngày tải lên :
08/08/2014, 21:20
... expression of TLR-2, -4, -5, -6, and -9 at the mRNA level (Figure 5) and, therefore, that CIN affects the activation of dendritic cells independently of TLR signalling Conclusion These studies demonstrate ... shows the percentage of cells that were positive for CD11c and for each of the markers Activation marker expression in CD11c+ cells from the bronchoalveolar lavage (BAL) fluid and lymph nodes of ... compared with untreated controls (Figure 1C); therefore, the decrease in IFN-c production observed in the CD8+ T cells does not appear to be the result of destruction of the cells but represents...
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