preparation of a high quality cdna library from a single cell quantity of mrna using chum rna
báo cáo khoa học: " Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system" pps
... bacterium was stimulated by aspartate Dashed lines show bacterial division points after 80 of stimulation by aspartate, localized Tar was diffused completely Then, the aspartate was removed from ... microchamber design, cell preparation, single cell observation, image analysis and drafted the manuscript DS and IK prepared Tar-GFP vector and discussed this study KY conceived of the study, and participated ... Yasuda: Analysis of singlecell differences using on-chip microculture system and optical trapping Fresenius' J Anal Chem 2001, 371:276-281 Umehara S, Wakamoto Y, Inoue I, Yasuda K: On-chip single- cell...
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Báo cáo y học: "Sustained High Quality of Life in a 5-Year Long Term Follow-up after Successful Ablation for Supra-Ventricular Tachycardia. Results from a large Retrospective Patient Cohort"
... diagnosis (Years) All patients AVNRT AVRT EAT From symptom to ablation (Years) All patients AVNRT AVRT EAT RF-Applications (Number) All patients AVNRT AVRT EAT Examination time (Minutes) All patients ... Abbreviations AVNRT: Atrio-Ventricular Nodal Reentry Tachycardia; AVRT: Atrio-Ventricular Reentry Tachycardia; AF: Atrial Fibrillation; EAT: Ectopic Atrial Tachycardia; F: French; INR: International ... EAT patients Panel A: Quantity and duration of episodes and the associated symptoms Panel B: Detraction in daily life generally and in parts of daily life variable PANAL A Detraction in daily...
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Báo cáo khoa học: Purification and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx
... Nilaparvata lugens Lucilia cuprina Anopheles dirus Bombyx mori Manduca sexta Anopheles gambiae Anopheles gambiae Blattella germanica Drosophila melanogaster Manduca sexta Anopheles gambiae Anopheles ... Removal of contaminating agents from the crude RNA extract was performed using a Qiagen RNeasy kit (Qiagen, Inc., Valencia, CA, USA) First-strand cDNA synthesis was carried out using a BD SMART RACE ... respectively Extraction of total RNA and cDNA synthesis Total RNA was isolated from adult German cockroaches using TRIzol reagent (Invitrogen Corp., Carlsbad, CA, USA), in accordance with the manufacturer’s...
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Báo cáo y học: "Screening of an endothelial cDNA library identifies the C-terminal region of Nedd5 as a novel autoantigen in systemic lupus erythematosus with psychiatric manifestations" ppt
... interpretation of data MR carried out the ELISA experiments and participated in analysis of data CA performed the statistical analysis and the clinical associations AS participated in the analysis and ... Psychiatric Association; 1994 14 Margutti P, Delunardo F, Sorice M, Valesini G, Alessandri C, Capoano R, Profumo E, Siracusano A, Salvati B, Rigano R, et al.: Screening of a HUAEC cDNA library ... were assayed Immunoscreening of the cDNA expression library A commercially available HUAEC cDNA library (Stratagene, Cambridge, UK) was used to screen for clones showing immunoreactivity with a...
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báo cáo khoa học: " A full-length enriched cDNA library and expressed sequence tag analysis of the parasitic weed, Striga hermonthica" pot
... Ishida J, Satou M, Sakurai T, Nakajima M, Enju A, Akiyama K, Oono Y, Muramatsu M, Hayashizaki Y, Kawai J, Carninci P, Itoh M, Ishii Y, Arakawa T, Shibata K, Shinagawa A, Shinozaki K: Functional annotation ... K, Kasuga M, Todaka D, Maruyama K, Nakashima K, Enju A, Mizukado S, Ahmed S, Yoshiwara K, Harada K, Tsubokura Y, Hayashi M, Sato S, Anai T, Ishimoto M, Funatsuki H, Teraishi M, Osaki M, Shinano ... 8:448 Ogihara Y, Mochida K, Kawaura K, Murai K, Seki M, Kamiya A, Shinozaki K, Carninci P, Hayashizaki Y, Shin IT, Kohara Y, Yamazaki Y: Construction of a full-length cDNA library from young...
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báo cáo khoa học: " An analysis of expressed sequence tags of developing castor endosperm using a full-length cDNA library" ppsx
... Akiyama K, Oono Y, Muramatsu M, Hayashizaki Y, Kawai J, Carninci P, Itoh M, Ishii Y, Arakawa T, Shibata K, Shinagawa A, Shinozaki K: Functional annotation of a full-length Arabidopsis cDNA collection ... Production of hydroxy fatty acids in the seeds of Arabidopsis thaliana Biochem Soc T Biochem Soc T 2000, 28:947-950 Seki M, Narusaka M, Kamiya A, Ishida J, Satou M, Sakurai T, Nakajima M, Enju A, Akiyama ... functional analysis of the cDNA clones, we constructed an oriented fulllength cDNA library in a lambda vector that incorporated the Gateway cloning system The quality of this library was assessed...
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Báo cáo khoa học: "The epidemiology of severe sepsis in England, Wales and Northern Ireland, 1996 to 2004: secondary analysis of a high quality clinical" pps
... frame and were unable to describe changes over time We present data from an analysis of a database arising from a national audit of patient outcomes from critical care units in England, Wales and ... interval Page of 10 (page number not for citation purposes) Case Mix Programme Database The Case Mix Programme Database is a high quality clinical database containing data on demographics, case ... care for England, Wales and Northern Ireland and is co-ordinated by the Intensive Care National Audit & Research Centre (ICNARC) [13] Patient data are abstracted by trained data collectors according...
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Báo cáo sinh học: "Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus" pps
... maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector, Proc Natl Acad Sci USA 89 (1992) 8794–8797 [21] Suzuki K., Asakawa S., Iida M., Shimanuki ... [18] Rogel-Gaillard C., Bourgeaux N., Billault A. , Vaiman M., Chardon P., Construction of a swine BAC library: application to the characterization and mapping of porcine type C endoviral elements, ... Katagiri T., Asakawa S., Hirono H., Aoki T., Shimizu N., Genomic bacterial artificial chromosome library of the Japanese flounder Paralichthys olivaceus, Mar Biotechnol (2000) 571–576 [13] Katagiri...
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ConstructionandCharacterizationofaFulllengthcDNALibraryandIdentificationof GenesInvolvedinSalinityStressinWild Eggplant(SolanumtorvumSwartz)
... ribosomal 28S and 18S -1 RNA; the concentration of total RNA was 1.365 µgµL , and the ratio of A2 60 /A2 80 of total RNA was 1.954 These data showed that high- quality total RNA was successfully isolated ... Meng, Y Kawamitsu, N Uehara, N Matsuda, Y Tanaka, H Ishikawa, S Baba, T Takabe, K Wada, T Ishii, and T Takabe 2001 Molecular cloning and functional characterization of two kinds of betaine-aldehyde ... electrophoretogram of total RNA extracted from the leaves of ‘Torvum Vigor’ M, pHY DNA size marker (Takara) Fig Agarose gel (1%) electrophoretogram of the purified mRNA from the total RNA in the leaves of...
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Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
... on the aromatic moiety of dibenzoylhydrazines on larvicidal activity against the Colorado potato beetle Leptinotarsa decemlineata Pest Manag Sci 57, 858–865 Nakagawa Y, Minakuchi C, Takahashi K ... Ogura T, Minakuchi C, Nakagawa Y, Smagghe G & Miyagawa H (2005) Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato ... the absence of hormone (assigned a value of 1.0), to allow for direct comparison of quantitative transcriptional activity All data points are based on n = 3; error bars indicate one standard...
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Tài liệu Báo cáo khoa học: A novel nuclear DNA helicase with high specific activity from Pisum sativum catalytically translocates in the 3¢fi5¢ direction docx
... cross-react with antibodies against plant helicases including PDH45 and PDH65 and also against human DNA helicases I, II, III and IV (data not shown) ssDNA-dependent ATPase activity was present at a ... and ssDNA cellulose were from Whatman and Pharmacia; T4 polynucleotide kinase and DNA polymerase I were from New England Biolabs; trypsin was from Serva (Heidelberg, Germany); the DNA-intercalating ... with varying concentration of MgCl2 (A) , KCl (B), or ATP (C) The concentrations used are given at the top of each lane of each gel The quantitative data are displayed on the left side of each autoradiogram...
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Tài liệu Báo cáo khoa học: "An Ensemble Method for Selection of High Quality Parses" pdf
... each trained on a different sample from the training data, to predict the quality of a parse The higher the agreement, the higher the quality of the parse Our approach assumes that if the parameters ... reranker Reranking and SEPA are thus relatively independent Bagging (Breiman, 1996) uses an ensemble of instances of a model, each trained on a sample of the training data1 Bagging was suggested in ... search the list of best parses output by a generative parser to find a parse of higher quality than the parse selected by the generative parser Thus, these algorithms in effect assess parse quality...
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Tài liệu The Economic Promise of Investing in High-Quality Preschool: Using Early Education to Improve Economic Growth and the Fiscal Sustainability of States and the Nation pptx
... international average on both academic and applied assessments of reading, math, and science, and rank among the lowest of more than 20 economically DEMOGRAPHIC CHALLENGES Favorable demographic and educational ... than those and 2000 Nobel Laureate in Economics most advantaged families aimed at remediation of The academic achievement past academic of advantaged students deficiencies Unless exceeds that of ... based on a number of parental and family circumstance factors were enrolled in the Carolina Abecedarian program The infants, who were primarily African American, either received early care and...
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Tài liệu Báo cáo khoa học: A single amino acid substitution of Leu130Ile in snake DNases I contributes to the acquisition of thermal stability A clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates docx
... DNase I cDNA was prepared from the total RNA derived from the pancreas by RT-PCR amplification using an Expanded High Fidelity PCR system with a set of two primers, 5¢-GAATTCGAGGCC ATGAAGACCATCTTG-3¢ ... quadrivirgata was prepared as described previously [15] Construction of the cDNA species encoding E quadrivirgata, E climacophora and A blomhoffii DNases I Total RNA was isolated from each snake ... obtained from the Japan Snake Institute, Gunma, Japan Phenyl Sepharose CL-4B, DEAE Sepharose CL-6B and Superdex 75 were purchased from Amersham Pharmacia Biotech; Concanavalin A (Con A) agarose...
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Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
... STIM1 domains gate and regulate Orai channels Nat Cell Biol 11, 337–343 Spassova MA, Soboloff J, He LP, Xu W, Dziadek MA & Gill DL (2006) STIM1 has a plasma membrane role in the activation of store-operated ... conformational changes in this motif The Ca2+ signal change and the accompanying conformational change in the canonical EF-hand are probably relayed to the SAM domain via the paired ‘hidden’ EF-hand, resulting ... via a short antiparallel b-sheet Ca2+ is coordinated by the mainchain carbonyl and side-chain carboxyl oxygens at the 12- or 14-residue loop One pair of EF-hands usually forms a globular domain...
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Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt
... peptide of MeMIH-B was amplified by PCR using T7 promoter-linked primers (forward, 5¢-TAATACGACTCACTATAGGTACTATG TATCGCATGCCAAT-3¢; reverse, 5¢-TAATACGACTC ACTATAGGTACTTTAAAGTCCCGGGTTGA-3¢) For ... successful RNAi has been reported for many animal models For example, Caenorhabditis elegans can be soaked in dsRNA or can be fed plasmids that make dsRNA and consequently exhibit RNAi effects In many ... Huberman A, Ramos L & Estrada MP (2006) Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference...
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Báo cáo khoa học: Characterization of SCP-2 from Euphorbia lagascae reveals that a single Leu/Met exchange enhances sterol transfer activity pdf
... (5¢-ATG AAAGTTCAAGAGAAGATCAATCTC-3¢); ELM99 (5¢ATGAGGGGTGCTATGAAGATCAAGG-3¢); ATL100 (5¢-ATTAGGGGTGCGCTGAAGATCAAGG-3¢); ATL14L15F18 (5¢-AAATCCGATGCAATCCTGGACCTGCTG AAGGAATTTCTTTCCACCGACGCC-3¢) For ... palmitic acid to the sample The values are mean ± SD from at least four different analyses Plant Type Ergosterol Palmitic Acid E lagascae E lagascae E lagascae E lagascae A thaliana A thaliana ... was amplified from E lagascae cDNA by the use of primers SCPElNE (5¢-ACTGGAATTCAACT CAAGTCCCAAAATATTTTGGAT-3¢) and SCPElCN (5¢-TCATGGCGGCCGCTCACAGCTTCGACGGCTTGG GGAA-3¢) The PCR fragment obtained...
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Báo cáo khoa học: Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ pdf
... 5¢-CAAAAAATTGAATGGCATG AACCGCCGAGCTCCAAC-3¢ and a2 : 5¢-AGCTTCAA AAATATCATTTAAACCCGACGGGCTGCTTTT-3¢ (the sequences for the biotin tag are underlined) followed by circularization with DNA ligase Preparation ... The DNA fragment coding the scFvLH (10 pg) was amplified with 0.4 lM each of the primers s1: 5¢-CTACC AGATCTGCCATGCAGATCGTTGTTACCCAGG-3¢ and a1 : 5¢-GGCTAAGAGCTCACGGTCAGGCTCG-3¢ by using a LATaq PCR ... was amplified on the pEU–scFvLH using SP6 primer (5¢-ATTTAGGTGACACTATAG-3¢) and anti-primer (5¢-ATGGCGCCAGCTGCAGGCTA-3¢, anti-stop codon in bold), and transcribed in the same way as above Translation...
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preparation of fe2o3 submicro - flowers by a hydrothermal approach
... through a hydrothermal method using Span80 or L113B as a soft template [17] Wan et al have proposed a soft-template-assisted hydrothermal route to prepare single crystal nanorods with an average diameter ... Probably due to the fine particle size and large surface area, the material shows superior electrochemical performance, with a high initial capacity of 905.8 mAh/g and good capacity retention of ... the sample is composed of uniform spheres with an average diameter of about 200 nm Fig 2(b) contains a SEM image at a higher magnification, from which it can be seen that there are some features...
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preparation of hexagonal wo3 from hexagonal ammonium tungsten bronze for sensing nh3
... instrument consisting of a XR3E2 X-ray source, a twin anode (Mg Ka and Al Ka) and a CLAM hemispherical analyser using Mg Ka radiation Detailed scans were recorded with 50 eV pass energy at (0.05 eV/1.5 ... detector using Cu Ka radiation In situ high temperature XRD patterns of h-WO3 in static air were collected by the same X-ray diffractometer in an Anton Paar HTK2000 high temperature XRD chamber using ... bands at 325, 300 and 264 cmÀ1 could be assigned to O–W–O deformation vibrations The band at 456 cmÀ1 was related to a small amount of reduced W atoms The peak at 184 cmÀ1 was a lattice mode of...
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