preparation microarray hybridization and scanning
Báo cáo y học: "Polarized expression of the membrane ASP protein derived from HIV-1 antisense transcription in T cells" potx
... the research; IC, SL, CT, SL, SB, AV, CAA, and BG performed research and collected data, BB and JMM analyzed data and wrote the paper All authors read and approved the final manuscript Competing ... analysis, and normal-sized mature virions were found in preparations of WT and ASPmut12 particles (Figure 1C) The presence of unambiguous coneshaped nucleoids was also observed in WT and ASPmut12 ... reporter gene and deficient for Env and Vpr synthesis [26]) was generously provided by Dr N.R Landau The pNL4.3AsLucE-R- vector has previously been described [7] The Flag-ASP, Flag-ASPΔATG, and FlagASPmut66...
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Báo cáo y học: "Biochip sensors for the rapid and sensitive detection of viral disease" pps
... Sample preparation and hybridization DNA array Scanning of array Protein array Image analysis Figure The structure of microarray experiments (a) To obtain gene-expression profile data from a cDNA microarray, ... smallpox and has two subtypes, variola major and variola minor, of differing pathogenicity Laassri et al [7] addressed the problem Infected cell (a) (b) RNA PROTEIN Sample preparation and hybridization ... and host chips, and each can be applied not only to detection and identification but also to the monitoring of viral populations In 1999, we and colleagues [6] described the first viral DNA microarray...
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Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf
... Q219), HQTQQK (loricrin, Q305), SSQQQKQ (SPR1, Q5 and Q7) and SQQVTQT (loricrin, Q215 and Q216) were determined as cross-linking sites by TGase and TGase 3, respectively However, differences in ... the prostate and is reported to be involved in plug formation in rodents [10] The biochemical characterization and physiological roles of TGase (expressed in keratinocytes), TGase and TGase remain ... situ enzymatic activity in both human and mouse skin [16,19] These studies have provided new insight into the substrate specificity of Tgases and have expanded the range of application of the...
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Báo cáo sinh học: " Biochemical prevention and treatment of viral infections – A new paradigm in medicine for infectious diseases" doc
... research and development Several strategies have emerged over the years and are being tested pre-clinically and clinically They include: antisense-oligonucleotides (AS-ONs), ribozymes, and recently, ... respiratory tract infection in infants and young children producing bronchiolitis and pneumonia worldwide RSV infection leads to more than 90,000 hospitalizations and a 2% mortality rate among infants ... preclinical and clinical development of CFY196 is warranted to fully evaluate its potential as a prophylaxis and therapeutics for the HRV induced common colds Biochemical Prevention and Treatment...
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Báo cáo sinh học: " CODEHOP-mediated PCR – A powerful technique for the identification and characterization of viral genomes" pptx
... presented (in bold and boxed) and the other amino acids found at each position are aligned vertically above the consensus amino acid The coding strand sequence and the complementary strand corresponding ... presented (in bold and boxed) and the other amino acids found at each position are aligned vertically above the consensus amino acid The sequences of the coding strand and complementary strand corresponding ... in the text The CODEHOP primers, YGDTB, IYG and GDTD1B are all derived from the antisense DNA strand and are shown below the codons for the sense strand Parameters for refinement of the "TVG-IYG"...
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báo cáo hóa học:" Biochemical prevention and treatment of viral infections – A new paradigm in medicine for infectious diseases" pdf
... research and development Several strategies have emerged over the years and are being tested pre-clinically and clinically They include: antisense-oligonucleotides (AS-ONs), ribozymes, and recently, ... respiratory tract infection in infants and young children producing bronchiolitis and pneumonia worldwide RSV infection leads to more than 90,000 hospitalizations and a 2% mortality rate among infants ... preclinical and clinical development of CFY196 is warranted to fully evaluate its potential as a prophylaxis and therapeutics for the HRV induced common colds Biochemical Prevention and Treatment...
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báo cáo khoa học: " Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen" pot
... SDS-PAGE gel and processed essentially according to Shevchenko [58] Bands were sliced into mm3-pieces Bands at 14, 19 and 35 kDa were cut out and analyzed as well Proteins were reduced with DTT and alkylated ... isoform 02C01 and comprises 17% of all fragment IIIvariants, while peptides IIIb and Xb are unique for 01B01 and comprise 1819% of all fragment III and X-variants The isoforms 02A01 and 02B01 could ... Da) and VIIIa (1854,89 Da), and Ij (1840,89 Da) and VIIIc (1840,88 Da) had a similar mass Fragment VIIIk overlaps with a keratin peptide * Fragments Xa and Xg have exactly the same mass and cannot...
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Báo cáo y học: "Eukaryotic large nucleo-cytoplasmic DNA viruses: Clusters of orthologous genes and reconstruction of viral genome evolution" pps
... humans and other mammals These viruses infect animals and diverse unicellular eukaryotes, and replicate either exclusively in the cytoplasm of the host cells, or possess both cytoplasmic and nuclear ... 130-380 170 100-220 150-190 100-220 1,180 370 Nucleus and cytoplasm Cytoplasm Cytoplasm Nucleus and cytoplasm Nucleus and cytoplasm Cytoplasm Nucleus and cytoplasm(?) Page of 13 (page number not for ... conserved NCLDV genes and the homologs of host genes encoded by the mimivirus indicate that the Mimivirus is a bona fide NCLDV and appears to be related to phycodnaviruses and iridoviruses [6]...
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Báo cáo y học: " Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies" potx
... Diagnostics and the NCRR grant to the Yerkes National Primate Center DRR000165 and to RHS, grants from NIH (NCI) CA103943, US Department of Defense W81XWH-07-1-338; and Mal and Lea Bank and (to RHS and ... Institute, Cleveland Clinic Foundation, 9500 Euclid Av, Cleveland, OH, 44195, USA 4Glickman Urological and Kidney Institute and LRI, Cleveland Clinic Foundation, 9500 Euclid Av, Cleveland, OH, 44195, ... HIV and HTLV are also primarily to the envelope proteins (HIV-gp120, HTLV-gp46), transmembrane proteins (HIV-gp41, HTLV-gp21), and core proteins of capsid and matrix (HIV-p24 and p17, HTLVp24 and...
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Báo cáo y học: "Oral keratinocytes support non-replicative infection and transfer of harbored HIV-1 to permissive cells" ppsx
... min, 95°C, min, and 95°C, 15s and 60°C, 30s, for 50 cycles 50°C, min, 95°C, min, and 95°C, 15s and 60°C, 30s, for 50 cycles 95°C, and 95°C, s, 54°C, 10 s, 72°C, s for 40 cycles 95°C, and 95°C, 10 ... of the study KR and MH conceived of the study, contributed to the design and coordination of the experiments, and critically reviewed and edited the manuscript All authors read and approved the ... trypsinization and were sensitive to cold and colchicine appeared to be harbored within TERT-2 cells and transferred to MOLT4/CCR5 cells by direct cell-to-cell interactions Discussion Lining the oral and...
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Báo cáo y học: "Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model" pptx
... were further amplified by PCR to add BamH1 and Bsp1407I sites at the 5' and 3' end of the fusion constructs, respectively and were ligated between BamH1 and Bsp1407I sites of pEFGFP vector [27] ... further amplified by PCR to delete a stop codon and to add KpnI and BamHI sites at the 5'- and 3'- termini, respectively The pEF/RT1AlSCTax180L-EGFP and pEF/ RT1AlSCNLEnv371L-EGFP vectors were generated ... confocal microscope system and a pair of GFP and CD8 images was overlaid (merge) Arrowheads indicate SCT-EGFP in 4O1/C8 cells Arrows indicate co-localization of SCT-EGFP and CD8 at the contact site...
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Báo cáo y học: "Common principles and intermediates of viral protein-mediated fusion: the HIV-1 paradigm" pps
... stalk-pore model of lipid bilayer fusion (A) and consensus models for class I and class II protein-mediated membrane fusion (B and C) SU and TM are the surface and transmembrane subunits of a fusion ... fusion between single HIV-1 and Avian Sarcoma and Leukosis Virus (ASLV) particles and target cells [48,49] Model systems for studying viral fusion Entry pathways and modes of activation While ... proximity of the MSDs and fusion peptides in the conserved hairpin structures and from extensive biochemical and functional data (Fig 1B, C) When triggered by receptor binding and/ or by low pH,...
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Báo cáo y học: " PRMT6 diminishes HIV-1 Rev binding to and export of viral RNA" pps
... recombinant histidine-tagged Tat86 and Rev Both sequences are chimeric and consist of BH10 (amino acids 2–66 and 2–15, respectively) and HXB2 (amino acids 67–86 and 16–116, respectively) Underscored ... control and R35K was not detectable The R39A and R39K substitutions resulted in band intensities that were either undetectable or 3% of wild-type, respectively Methylation of the R38A and R38K ... the manuscript BX cloned and isolated Tat86, PRMT6, and wild-type TatRev fusion protein, and stably transfected HeLa cells with siRNA directed against PRMT6 or mock siRNA, and carried out the experiments...
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Báo cáo sinh học: "Analysis of a simulated microarray dataset: Comparison of methods for data normalisation and detection of differential expression (Open Access publication)" pps
... gene id (gene), mean and standard deviation of the unnormalised log ratio (M and SD), mean and standard deviation of the LOESS normalised log ratio (M LOESS and SD LOESS) and the number of methods ... contains the gene id (gene), mean and standard deviation of the unnormalised log ratio (M and SD), mean and standard deviation of the LOESS normalised log ratio (M LOESS and SD LOESS), the number of ... normalised data, and (C) LOESS followed by scale normalised data column and found not needed) and INRA_J (correction by block) applied any further spatial normalisation SLN1 and SLN2 applied...
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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples ... recovery of soyasapogenols A and B without producing artifacts (Ireland and Dziedzic 1986; Rupasinghe, Jackson et al 2003) Moreover, Ireland and Dziedzic (Ireland and Dziedzic 1986) showed that ... confounding authentic saponin identification and quantification The purification process is complex and laborious with long sample preparation and extraction times and yielding relatively low amounts...
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Analysis and detection of human motion in time frequency domain
... falls (left hand 126 side and right hand side) and backward fall 5.14 Typical body configuration at threshold level for backward 127 14 fall and sideways fall 5.15 The mean and one side standard deviation ... walking, ascending stairs and descending stairs However, in sitting, standing, and lying down detection, instead of detecting static postures, we detect stand-sit/sit-stand, and liesit/sit-lie posture ... activity) (1 = left hand side fall (FW), = left hand side fall (S), = right hand side fall (FW), = right hand side fall (S), and 5= backward fall (RU)) 5.16 The angular rate of right hand side fall...
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Analysis and detection of human emotion and stress from speech signals
... pair-wise and grouped classifications using data from ESMBS (Emotional Speech of Mandarin and Burmese Speakers) emotion database that is build for this study and SUSAS (Speech Under Simulated and Actual ... signal-to-noise-ratio 46 Time waveforms and respective spectrograms of Disgust and Fear emotions of Burmese and Mandarin speakers from emotion database in noise free and noisy conditions Noise is additive ... Chapter 5: Subband Based Feature Extraction Methods and Analysis 5.1 Selection of Stress and Emotion Classification Features 5.2 79 80 Feature Extraction Techniques for Stress and Emotion Classification...
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Application of support vector machine to prediction of cross reactive allergens and recognition of viral sequences
... networks (McCulloch and Pitts, 1943), nearestneighbour rule (Fix and Hodges, 1951), Bayesian classifiers (Duda and Hart, 1973), hidden Markov model (Baum and Petrie, 1966) and support vector machines ... mutational biases and natural selection thus being non-random (Sharp and Matassi, 1994; Clarke, 1970) Analysis of codon usage in mammalian, viral, bacteriophage, bacterial, mitochondrial and lower eukaryote ... APPEL employs SVM and sequence-derived protein structural and physicochemical properties as feature vectors (Cui et al., 2005) Cui and coworkers trained APPEL using known allergens and putative non-allergens...
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Comparative studies on the infection and colonization of maize leaves by fusarium graminearum, f proliferatum and f verticillioides
... with5mLofsporesuspensionatthe56leafstageasdescribedaboveandmaintained in the growth chamber at 1820oC and 2224 oC, and 60 and 80%, relative humidity respectively and a dayand night photoperiod of 15hours. Control ... 4.AssessmentofinfectionbyFusariumgraminearum,F.proliferatumandF. verticillioidesonmaizeleavesusingquantitativePCRandmicrobiologicalassays 93 4.1.Introduction 93 4.2.Materialsandmethods 95 4.2.1.Fungalpathogenandinoculumpreparation ... 5thdaibutafterwardsdecreasedfromthe5thdaitothe20thdaiandincreasedagainuntil the40thdai.Diseaseseverityandfungalbiomass,diseaseseverityandcolonizationofthe6th and7 thleavesweresignificantlypositivecorrelationat10daiand40dai,respectively....
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