prbp pdedelta interacts with many proteins
Báo cáo khoa học: Mouse cytosolic sulfotransferase SULT2B1b interacts with cytoskeletal proteins via a proline⁄serine-rich C-terminus doc
... C-terminus may facilitate interactions with other intracellular proteins rather than executing a catalytic reaction mSULT2B1b interacts with cytoskeletal proteins through its proline ⁄ serine-rich ... serine-rich C-terminal tail of mSULT2B1b may interact with other intracellular proteins, and set out to identify the proteins that it may react with The extended C-terminus is not involved in catalytic ... et al Interaction of SULT2B1b with cytoskeletal proteins using MALDI-TOF MS The results shown in Table revealed that, of the five proteins, three were cytoskeletal proteins (actin, a-actinin, and...
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Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf
... (uniprotkb:Q9R064) physically interacts (MI:0915) with MT1-MMP (uniprotkb:P50281) by two hybrid (MI:0018) MINT-7898002: MT1-MMP (uniprotkb:P50281) physically interacts (MI:0914) with Furin (uniprotkb:P09958) ... (uniprotkb:Q9R064) physically interacts (MI:0915) with Furin (uniprotkb:P09958) by two hybrid (MI:0018) MINT-7898052, MINT-7897410: MT1-MMP (uniprotkb:P50281) physically interacts (MI:0915) with GRASP55 (uniprotkb:Q9R064) ... (uniprotkb:Q9R064) physically interacts (MI:0915) with PC7 (uniprotkb:Q16549) by two hybrid (MI:0018) MINT-7897866: GRASP55 (uniprotkb:Q9R064) physically interacts (MI:0915) with MT5MMP (uniprotkb:Q9Y5R2)...
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Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf
... functional link between several proteins involved in prerRNA processing The exosome subunit Rrp43p interacts with Rrp46p, Nip7p and Nop17p [10,13,28] Nop17p interacts with Nop58p and Nop53p [28] ... with the snoRNPs and to participate in cleavage reactions as well as methylation and pseudouridylation of specific nucleotides of rRNA [24–28] Among these proteins is Nop17p, which interacts with ... associated with the core proteins Cbf5p, Gar1p, Nhp2p and Nop10p and function in the conversion of uridine into pseudouridine [17–23] In addition to the core snoRNP proteins, other proteins have...
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Báo cáo khoa học: CD91 interacts with mannan-binding lectin (MBL) through the MBL-associated serine protease-binding site doc
... CD91 interacts with MBL at or near the MASP-binding site (A) Using SPR, nM of MBL was injected over a sensor chip on which CD91 was immobilized (16 000 RU), with or without preincubation with ... immobilized (14 600 RU) RU, resonance units CD91 interacts with MBL at or near the MASP binding site It has previously been shown that calreticulin interacts with MBL at the MASP-binding site [28] FEBS ... the binding sites on C1q for CD91 were shared with calreticulin [19] Here, it is shown that CD91 interacts directly with MBL and, to a lesser extent, with L-ficolin and therefore has the potential...
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Báo cáo khoa học: A zinc finger HIT domain-containing protein, ZNHIT-1, interacts with orphan nuclear hormone receptor Rev-erbb and removes Rev-erbb-induced inhibition of apoCIII transcription potx
... high identity with its homologues (100% with Canis familiaris, 97% with Mus musculus, 87% with Xenopus tropicalis, 87% with Danio rerio, 68% with Drosophila melanogaster and 33% with Schizosaccharomyces ... interacted with Rev-erbb The zinc finger HIT domain is a motif found in many proteins, and plays an important role in gene regulation and chromatin remodelling [25] So far, five human proteins containing ... transfected with plasmids pEGFP-Rev-erbb and pDsRed-ZNHIT-1, or cotransfected with both plasmids After 36 h of transfection, the cells were fixed with 4% formaldehyde and stained with lgÆmL)1...
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Báo cáo khoa học: The chaperone and potential mannan-binding lectin (MBL) co-receptor calreticulin interacts with MBL through the binding site for MBL-associated serine proteases pdf
... buffer with EDTA added to test whether Ca2+ was a limiting factor, and in TN buffer (25 mm Tris, 0.15 m NaCl, pH 7.5) with Ca2+ added Control experiments with non-coated wells and wells coated with ... Interaction of calreticulin with MBL bound to immobilized mannan Wells were coated as indicated with rMBL and pMBL (1 lgÆmL)1), or with mannan (1 mgÆmL)1) followed by incubation with rMBL and pMBL Subsequently, ... not bind MBL point mutants with defective MASP interaction Steinø et al [53] showed that calreticulin interacts strongly with immobilized C1q, whereas pMBL (associated with the MASPs) only exhibits...
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Báo cáo khoa học: Trigger factor interacts with the signal peptide of nascent Tat substrates but does not play a critical role in Tat-mediated export pptx
... away from L23 and L29 and interacts with TF Furthermore, the combined data obtained with nascent TorA/P2 and SufI constructs strongly suggest that interaction of TF with the signal peptide early ... material was immunoprecipitated with TF antiserum as indicated An 170 kDa complex that could be precipitated with anti-HA, but not with anti-TF serum is indicated with an asterisk Samples were ... the crosslinked partners are peripheral membrane proteins and not one of the known Tat proteins [25] The adducts could not be immunoprecipitated with various antisera tested and remain to be identified...
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Báo cáo Y học: Double-stranded RNA-activated protein kinase interacts with apoptosis signal-regulating kinase 1 Implications for apoptosis signaling pathways docx
... analysis was performed with Kodak DIGITAL SCIENCE 1D software (Eastman Kodak) PKR interacts with ASK1 (Eur J Biochem 269) 6127 washed four times with PKR buffer I and then once with PKR buffer III ... RESULTS PKR interacts with ASK1 Indirect immunofluorescence The day after transfection, cells were fixed with 4% paraformaldehyde containing 0.2% Triton X-100 in NaCl/Pi for 30 and washed with NaCl/Pi ... Takizawa et al (Eur J Biochem 269) Ó FEBS 2002 Fig PKR interacts with ASK1 (A) Cytoplasmic localization of ASK1 with PKR COS-1 cells were transfected with pcDNA-PKRKR (a), pcDNA-ASK1-HA (b), or both...
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Báo cáo khoa học: NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and integrin binding protein (CIB) and shows developmentally restricted expression in the neural tube pptx
... The NBR1 protein is highly conserved, with 87% sequence similarity with the murine homologue The murine Nbr1 gene lies head to head with the Brca1 gene, with the intragenic region of 289 bp suggesting ... D19 developer, ®xed with Kodak UNIFIX and counter stained with malachite green Sections were photographed under dark ®eld with an Olympus BH-2 microscope and photographed with an Olympus camera ... microscope (Zeiss) RESULTS NBR1 interacts with the PKC zeta interacting protein FEZ1 and the calcium and integrin binding protein CIB In order to identify proteins that interact with NBR1, a yeast two-hybrid...
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Báo cáo khoa học: Nucling interacts with nuclear factor-jB, regulating its cellular distribution potx
... NF-jB apparently overlapped with that of Nucling (Fig 5B,F) or NuclingDC (Fig 5C,G), but not with that of NuclingDN (Fig 5D,H), suggesting that Nucling interacts with NF-jB through its N-terminal ... detect IjBa in the lanes for bands a–c (Fig 4B) Thus, we concluded that Nucling interacts with NF-jB-p50 and possibly with NF-jB-p65 in primary cells To assess the potential of endogenous Nucling ... Taken together with the results of the reporter assays in this study, we concluded that Nucling is important for the regulation of the NF-jB signaling pathway Nucling interacts with NF-jB Considering...
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Báo cáo khoa học: UXT interacts with the transcriptional repressor protein EVI1 and suppresses cell transformation ppt
... for these two proteins To date, several Evi1-binding proteins have been identified using a candidate protein approach It Evi1 binding proteins remains possible that Evi1 interacts with as yet unknown ... A1 with Rp is specific because A1 does not interact with laminin, p53 (Fig 1A–C; pGBKT7Lam, pGAD10 A1 and pGBKT753, pGAD10 A1) or mCtBP2 (Fig 1D,E; pGBT9 A1 ⁄ pGAD10mCtBP2) identity of A1 with ... resolved by SDS–PAGE (10%) and sequentially probed with a-haemagglutinin (Boehringer Mannheim, Mannheim, Germany; WB a-12CA5) and a-myc (WB a-9E10) mAb Proteins were visualized by ECLTM (Amersham Pharmacia...
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Báo cáo khoa học: Ki-1⁄57 interacts with PRMT1 and is a substrate for arginine methylation pptx
... localization of the proteins Ki-1 ⁄ 57, CGI)55 and PRMT1 HeLa cells were grown on coverslips and incubated for 16 h with or without Adox or for h with actinomycin D at 37 °C, with the addition ... or physically with PRMT1, including tubulin [24] or ILF3 [36] Most of these proteins contain one (86%) or more (66%) RGG ⁄ RXR boxes (Table 1) Two of the proteins are ribosomal proteins that ... RACK1 interacts with p73, a paralog of p53, and that RACK1 reduces p73-mediated transcription by direct physical binding with it [12] Arginine methylation is a post-translational modification of proteins...
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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc
... nonreducing SDS/PAGE with (+) or without (–) 20 mM dithiothreitol in the sample buffer Detection of protein bands was carried out with a His6-antibody and chemiluminescence with the exception of ... reaction mixture was determined from a sample without enzyme Western analysis For immunological studies, aliquots with about 200 ng of purified Erv1p and mutant proteins were applied to 4%-12% nonreducing ... check the in vivo activity of the mutated proteins we used a haploid yeast strain with a temperature-sensitive Erv1 protein [12] Yeast cells were transformed with single copy pRS416 constructs containing...
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Báo cáo khoa học: Dematin interacts with the Ras-guanine nucleotide exchange factor Ras-GRF2 and modulates mitogen-activated protein kinase pathways doc
... regulation of Ras proteins Ras GTPases cycle between an active GTPbound state and an inactive GDP-bound state GTPase activating proteins (GAPs) catalyze the intrinsic GTPase activity of Ras proteins, ... domain that Sos is constitutively associated with the SH3 domain of the adaptor protein Grb2 [20] Grb2 protein also contains an SH2 domain that interacts with a phosphorylated tyrosine residue of ... transfected with either vector, or constitutively active Ras, or dematin, or Ras-GRF2 Cells were stimulated with ionomycin, as described in the Experimental procedures, and lysates were immunoblotted with...
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Báo cáo khoa học: Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA docx
... bound by four core proteins, Nop1p, Nop58p, Nop56p and Snu13p [8] In addition to the core proteins, the U3 snoRNP is associated with other proteins specific for this snoRNP The first proteins to be ... injected with 10 lg purified His6-Utp25 mixed with complete Freund’s adjuvant (Sigma, St Louis, MO, USA) each The mice were injected three more times with 1-week intervals with 10 lg purified protein with ... for interaction with Utp25p (Fig 9A, lower) 2844 Fig Utp25p interacts with SSU processome subunits (A) Utp25p was fused to lexA DNA-binding domain (BD) and tested for interaction with Mpp10p, Sas10p,...
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Báo cáo khoa học: Hepatocyte nuclear factor-4a interacts with other hepatocyte nuclear factors in regulating transthyretin gene expression pot
... reduced by treatment with cytokines HepG2 cells were treated with or without cytokines for 18 h The interaction of HNF protein with the DNA binding site was determined by ChIP assays with antibodies ... exogenous HNF-1a, HNF-3a, HNF-3b and HNF-6a on TTR transcription (comparing bar with 7, with 10, 12 with 13, and 15 with 16 in Fig 3) The loss of response to the overexpression of HNF-3a or HNF-3b ... the TTR promoter with WT or individually mutated HNF binding sites was incubated with nuclear protein extracted from HepG2 cells; the DNA–protein complexes were analyzed by ELISA with antibodies...
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Báo cáo khoa học: CmtR, a cadmium-sensing ArsR–SmtB repressor, cooperatively interacts with multiple operator sites to autorepress its transcription in Mycobacterium tuberculosis pptx
... A competition assay was performed with the F-1 fragment and lM of CmtR with no competitor (lane 2), with 50 · non-specific competitor [poly(dI-dC), lane 3], or with 10· (lane 4) and 50· (lane 5) ... CmtR interacts cooperatively with multiple binding sites that span 90 bp of sequence up- and downstream of its own translational start site ()80 to +10 bp) Our results show that CmtR interacts with ... sites, we performed EMSA with oligonucleotides of approximately 20 bp carrying the predicted binding sites 1–4 (Fig 5G) The EMSA results clearly show that CmtR protein interacts with each of these...
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Báo cáo khoa học: Weak toxin WTX from Naja kaouthia cobra venom interacts with both nicotinic and muscarinic acetylcholine receptors potx
... inhibited by native WTX with an IC50 of 2.95 lm (data not shown), in good agreement with the previously reported IC50 value Taken together, these data show that WTX interacts with both muscle-type ... interaction with the mAChRs, and that WTX does not interact directly with the G -proteins themselves: significant differences in [35S]GTP[cS] binding according to receptor subtype are not consistent with ... ligands, suggesting that WTX interacts with both nAChR and mAChR Experiments using heterologous expression of mAChRs revealed that the mechanism of WTX interaction with mAChRs is allosteric This...
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Báo cáo khoa học: A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2 pptx
... Nagasaki et al associated with the plasma and organelle membranes via covalent modification with lipids, formation of complexes with membrane integral proteins and association with membrane components ... G-protein heterotrimers, some small G -proteins and several non-receptor-type tyrosine kinases are N-myristoylated proteins Proteins with lipid modifications come in many shapes, sizes and functions, ... treatment with Na2CO3 removes peripheral membrane proteins, which are associated with membrane intrinsic proteins, and a mild detergent Triton X-100 is used to solubilize membrane proteins, but...
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Báo cáo khoa học: Peroxin Pex21p interacts with the C-terminal noncatalytic domain of yeast seryl-tRNA synthetase and forms a specific ternary complex with tRNASer potx
... have also been found to associate with aaRSs Yeast TyrRS interacts with Knr4, a protein involved in cell wall synthesis [29], and heat shock protein 90 interacts with human glutamyl-prolyl-tRNA ... His-tag Ni2+–nitrilotriacetic acid agarose precharged with Pex21p was incubated with yeast or maize SerRS variants Proteins bound to resin were eluted with buffer containing 300 mm imidazole and analyzed ... protein cofactor could bind tRNA, as interactions with two proteins might enable more efficient tRNA turnover We coincubated tRNASer and the proteins at 25 °C, and then performed gel-shift analysis,...
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