prbp pdedelta interacting proteins using prbp pdedelta sepharose beads
Báo cáo khoa học: Novel brain 14-3-3 interacting proteins involved in neurodegenerative disease doc
... were captured by incubation with glutathione Sepharose beads for h at °C After centrifugation, beads were washed four times with lysis buffer Bound proteins were eluted with SDS sample buffer ... applied to a GST 14-3-3 f column After extensive washing, bound proteins were eluted by directly boiling of the glutathione Sepharose beads in SDS ⁄ PAGE sample buffer and analysed by 6% SDS ⁄ PAGE ... isopropyl bd-thiogalactopyranoside for h at 25 °C The fusion proteins were purified by affinity chromatography on glutathione -Sepharose beads (Amersham Pharmacia Biotech.) For large-scale preparation...
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Báo cáo khoa học: The protein tyrosine phosphatase PTP-Basophil/Basophil-like Interacting proteins and molecular functions doc
... PTP-Bas/BL Knowledge about PTP-Bas/BL has increased due to the identification of a number of proteins interacting with one or two of the interaction domains present in PTP-Bas/BL A summary of these ... indicate that an association has been determined but direct interaction was not investigated The interacting proteins can be divided into three groups Known or potential regulators of the actin cytoskeleton ... are currently no proteins known to interact directly with the FERM domain of PTP-Bas/BL PDZ domains PDZ domains are able to interact selectively with the C-termini of their target proteins [21,35,36]...
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Báo cáo hóa học: " A comparative analysis of viral matrix proteins using disorder predictors" docx
... reflected in some peculiar features of the corresponding viral proteins This paper examines matrix proteins of several related viruses using computational tools such as intrinsic disorder predictors ... to proteins with known 3D-structure This approach revealed some peculiar patterns of PID that can be used to better understand behavior of the HIV matrix proteins Table 1: A summary of matrix proteins ... Disordered Matrix Proteins: Immune Evasion The question then arose: What are the potential implications of more rigid or more disordered matrix proteins? It is likely that more rigid p17 proteins may...
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Báo cáo hóa học: " Sensitive and molecular size-selective detection of proteins using a chip-based and heteroliganded gold nanoisland by localized surface plasmon resonance spectroscopy" pptx
... the proteins and the active sites of nanoholes Third, the transduction system was modified for application to a chip-based detection method This would premit the fabrication of materials using ... adsorption of proteins J Chem Phys 2004, 121:2264 33 Kim H, Cho IH, Park JH, Kim S, Paek SH, Noh J, Lee H: Analysis of a nonlabeling protein array on biotin modified gold surfaces using atomic ... 20:O25703 38 Han XX, Huang GG, Zhao B, Ozaki Y: Label-free highly sensitive etection of proteins in aqueous solutions using surface-enhanced Raman scattering Anal Chem 2009, 81:3329 39 Marinakos SM,...
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Báo cáo y học: "Comparison of metal-dependent catalysis by HIV-1 and ASV integrase proteins using a new and rapid, moderate throughput assay for joining activity in solution" ppt
... HIV-1 and ASV IN proteins at μM concentration, using our fluorescence joining assay (Figure 4) The concentration dependence for compound inhibition of joining by HIV-1 and ASV IN proteins is shown ... preparation The ASV and HIV-1 IN proteins used in these studies were purified from the soluble fraction of bacterial lysates after expression of untagged versions of the proteins from plasmid vectors ... biotin-conjugated target and joined products to bind to the beads The wells were then washed 10 times with 200 μl Wash Buffer (1× PBS, 0.05% SDS, mM EDTA) using a vacuum manifold (Pall Life Sciences; Multi-well...
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Báo cáo y học: "Development of a method for screening short-lived proteins using green fluorescent protein" pps
... developed an innovative technology to identify labile proteins using GFP-fusion expression libraries Using this technology we have discovered short-lived proteins in a high-throughput format This technology ... is consistent with the expectation that labile proteins tend to be of lower abundance than more stable proteins refereed research Turnover of the proteins associated with clone 19 and clone 25 ... fusion proteins diminished in the presence of CHX (Figure 4a) The half-life of the proteins determined by western blot analysis was similar to that determined by FACS analysis Two of the proteins...
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Báo cáo y học: "nferring protein domain interactions from databases of interacting proteins" ppt
... pair of interacting proteins is considered a potentially interacting domain pair (b) The frequency of proteins with domain i interacting with proteins with domain j, Sij is computed (c) Using Sij ... a pair of proteins is defined as potentially interacting if the proteins belong to the same organism Thus, Oτ ,y is only defined when proteins x and y both belong to x organism τ All proteins ... minority: the probability of two random proteins in a proteome interacting is quite small For example, in an organism with 6,000 proteins, each with an average of four interacting partners, the probability...
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Báo cáo y học: "Comparative analysis of Saccharomyces cerevisiae WW domains and their interacting proteins" pps
... respectively, using a binomial test) relative to all proteins present on the microarrays In the S cerevisiae proteome, approximately 250 proteins contain PY motifs (4% of all proteins) and 400 proteins ... introduced into cells, and interacting proteins would be identified using immunoprecipitation and western blotting or affinity interactions We searched the network for groups of proteins having conserved ... the proteins in our network for occurrences of these motifs Within the network, 28 proteins have canonical PY motifs and have poly-proline motifs Twentysix proteins have PPR motifs, and 38 proteins...
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Báo cáo y học: "Choosing the right path: enhancement of biologically relevant sets of genes or proteins using pathway structure" ppt
... (estimated using 1,000 random networks) are given in Table Significant Pvalues across the comparisons support our use of the DR as a reasonable score for differentiating between pathways Analysis using ... pathway network (Figure S1 in Additional data file 2) using the meta_DR term in Equation The P-value for the significance of meta_DR is computed using 1,000 random networks whose generation is described ... pathways found using the training set versus the test set Curves for sensitivity versus '1 - specificity' and positive predictive value versus negative predictive value are obtained by using different...
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Local induction of heat shock proteins using magnetic fluid hyperthermia for ocular neuroprotection in glaucoma
... LOCAL INDUCTION OF HEAT SHOCK PROTEINS USING MAGNETIC FLUID HYPERTHERMIA FOR OCULAR-NEUROPROTECTION IN GLAUCOMA MINHONG JEUN A THESIS ... modality for the ocular neuroprotection in glaucoma [26-28] The HSPs, called by stress proteins, are a group of proteins that exist in all of the living creatures covering bacteria to human beings ... Kit-8 Assay 3.4.2 TEM Study and Cellular Uptake 3.5 59 59 Identification of Induction of Heat Shock Proteins 72 - Cell Staining (Fluorescein Isothiocyanate and 4’, 6-Diamino-2Phenylindole) and Western...
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Regulation of cullin e3 ubiquitin ligases by the ubiquitin like protein nedd8 and cullin interacting proteins
... cellular quality control by eliminating defective proteins from the cytosol and endoplasmic reticulum These defective proteins include misfolded proteins, proteins that fail to assemble into complexes, ... products encode proteins that contained a conserved BTB/POZ domain In the same study, biochemical assays using the BTB protein MEL-26 and its genetic target MEI-1 further indicate that BTB proteins ... glutathioneSepharose 4B (Amersham Biosciences) was equilibrated with 10 column volume (CV) lysis buffer The cleared lysate was applied to the column and was mixed gently with glutathione -Sepharose beads...
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Identification and characterization of svp interacting proteins of short vegetative phase in arabidopsis thaliana
... Heat shock proteins 26 1.4.1 Heat shock proteins in plants 26 1.4.2 Hsp40 27 1.4.2.1 General features of Hsp40 27 ii 1.4.2.2 J-domain proteins in Arabidopsis 1.5 Function of J-domain proteins during ... modulate meristem determinacy 25 1.4 Heat Shock Proteins 1.4.1 Heat shock proteins in plants Heat shock proteins (Hsps), or also called as stress proteins, are present in virtually all living organisms, ... 1.4.2.2 J-domain proteins in Arabidopsis In Arabidopsis, there are a large number of J-domain proteins identified (Miernyk, 2001; Rajan and D'Silva, 2009) A total of 89 J-domain proteins were identified...
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Mapping the binding site of ligands to proteins using chemical exchange parameters by NMR spectroscopy
... ‘Bax like’ proteins and ‘BH3 only proteins The ‘Bax like’ proteins contain three BH domains namely, BH1 through BH3, but lack the BH4 domain additionally present in the pro-survival proteins Bax, ... proteins which regulate (stimulate/inhibit) the activity of the apoptosome and caspases at various levels[50] Bcl-2 family proteins are broadly classified into pro-survival and pro-apoptotic proteins ... Classification of BCL-2 family proteins 12 1.7 Sequence alignment of Pro-survival and pro-apoptotic proteins 13 1.8 Sequence alignment of pro-apoptotic proteins ...
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Báo cáo y học: "Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System"
... Membrane Proteins Interacting with HBsAg A number of cellular proteins have been isolated using the conventional yeast two hybrid screening [10, 11] However, these reported HBV interacting proteins ... cDNA library using the yeast membrane proteins in vivo Proc Natl Acad Sci USA 1998; 95: 5187split-ubiquitin system Sequence analysis revealed three 5192 interacting cellular membrane proteins as ... to comprehensively isolate and characterize, in an environment, cellular proteins interacting not only with soluble HBV proteins, but also with membrane bound HBsAg To this end, a linearised form...
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Interacting with the Outside World Using Simple IO Devices
... ****************************************************************************** 22 Interacting with the Outside World Using Simple I/O Devices Program 2.3 LED ON-OFF by using shift operators This application is one step towards ... 20 Interacting with the Outside World Using Simple I/O Devices show pieces, etc Many of the embedded system products ... connected to port B and outputs the same on LEDS, which are connected to port D */ 24 Interacting with the Outside World Using Simple I/O Devices // Data Lines (DIP switched is connected to PORT B //...
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Báo cáo khoa học: Accumulation of polyubiquitinated proteins by overexpression of RBCC protein interacting with docx
... to glutathione Sepharose beads, incubated with Lys48-linked polyubiquitin chains, and subjected to a GST pull-down assay The polyubiquitin chains pulled down with the GST-fused proteins were detected ... of cellular functions mediated by Lys63-linked polyubiquitinated proteins Splice variants of RING proteins More than 1000 RING proteins have so far been found in eukaryotic cells, and many of ... against FLAG Whereas endogenous proteins were polyubiquitinated, which was not detected under reduced sensitivity (Fig 1B, lane 1), a significant amount of polyubiquitinated proteins was found to unexpectedly...
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Báo cáo khoa học: " Inhibition of Tomato Yellow Leaf Curl Virus (TYLCV) using whey proteins" doc
... transferring using Agro-Infiltration with TYLCV-IS infectious clone After 10 - 20 days from infection, each plant was sprayed using 20 ml of the native and chemically modified whey proteins fraction ... using the modified Dellaporta extraction method [23,24] Antiviral activity of modified whey proteins fractions Antiviral activity was assessed on TYLCV particles replicated in plant tissue, using ... esterification of milk proteins Milchwissenschaft 2001, 56:127-131 Ryan DS: Determinants of the functional properties of proteins and protein derivatives in foods in Food proteins Improvement through...
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Báo cáo y học: "Psoriasin, one of several new proteins identified in nasal lavage fluid from allergic and non-allergic individuals using 2-dimensional gel electrophoresis and mass spectrometry" potx
... Uppsala, Sweden) In-gel rehydration and isoelectric focusing (IEF) was performed over night (~60000 Vh) using Ettan IPGphor Isoelectric Focusing System (Amersham Biosciences, Uppsala, Sweden) After ... an overview of which proteins that can be of importance for the development of allergic diseases Most of the 21 proteins identified in the present study correlate well with proteins found in NLF ... samples In this study both previously identified and newly identified proteins were detected in NLF using this method Some of these proteins, like psoriasin, Wnt-2B and PIP were found to be differently...
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Báo cáo y học: "Host proteins interacting with the Moloney murine leukemia virus integrase: Multiple transcriptional regulators and chromatin binding factors" pot
... of interacting proteins in the screens: Group I, transcription factors and chromatin binding proteins; Group II, RNA binding proteins; and Group III, miscellaneous proteins A subset of the proteins ... factors and DNA binding proteins We have examined interactions between 18 of these proteins in vitro using binding assays with both MoMLV and HIV-1 integrases Of the 18 proteins examined in vitro, ... Polycomb and Trithorax proteins will reveal a role for these proteins in retroviral integration, given that Trithorax proteins are transcriptional activators and Polycomb proteins are transcriptional...
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Báo cáo y học: "Functionally important segments in proteins dissected using Gene Ontology and geometric clustering of peptide fragments" potx
... to assess function [20] One such recent study [21] identifies function-associated loops in proteins using Gene Ontology (GO) [22] molecular function (MF) terms In this case, the starting information ... relationship to experimentally determined known functional sites in proteins A recheck of the results with FL16 fragments using level GO MF terms showed occasional overlap with FL8 results, indicating ... indicate residues putatively interacting with the guanidinium group of agmatine Green inverted triangles indicate the residues observed in the crystal structure to be interacting with the bound...
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