practical strategies for protein contaminant detection by high performance ion exchange chromatography
Simultaneous determination of sildenafil, vardenafil and tadalafil as forbidden components in natural dietary supplements for male sexual potency by high performance liquid chromatography–electrospray ionization
Ngày tải lên :
02/09/2015, 13:06
... applied So, high concentration of modifier was not recommended 3.2 MS conditions The MS parameters were optimized attentively by flow injection analysis (FIA) ESI is a soft ionization technique, ... obtained by employing UV detection at 292 nm dards to three blank samples (wine, beverage, oral liquid formulation) at low, medium and high levels Each sample of the same concentration was injected ... selective ionization recording (SIR), the cone voltages for sildenafil, vardenafil and tadalafil were set at 50, 50, and 20 V, respectively 2.5 Linearity, limit of detection, limit of quantification The...
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Strategies for learning english writing by 10th form students at high schools = các chiến lược ngôn ngữ được ứng dụng trong việc học môn viết tiếng anh cho học sinh lớp 10 tại các trường THPT
Ngày tải lên :
20/12/2013, 18:33
... and to ask questions for clarification The subcategories of social strategies are: • Asking questions strategies: asking for clarification or verification, and asking for correction 16 • Co-operating ... 25 • Edit for truth and accuracy • Edit for organization • Edit for paragraph structure • Edit for sentence structure • Edit for word choice • Edit for spelling and punctuation • Edit for conciseness ... memory and retrieving information when needed for communication” (Oxford, 1990, cited in Clouston 1997) i.e Memory strategies enable learners to store and retrieve new information They are broken...
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Báo cáo hóa học: " Research Article Hardware/Software Codesign in a Compact Ion Mobility Spectrometer Sensor System for Subsurface Contaminant Detection" ppt
Ngày tải lên :
22/06/2014, 00:20
... for flexibility in the duration of the extraction and injection states, so that the system can be optimized for differing environmental conditions A single 32-bit control word contains extraction ... fully optimized The high performance FPGA DSP allows for upgrades and higher-order digital filtering techniques that can help improve the signal-to-noise ratio (SNR) and, therefore, the resolving ... into the ionization region of the IMS sensor In the data acquisition stage, the ion gate is cycled open and closed Sin Ming Loo et al RS232 Solenoid drivers FPGA A/D Gate controller High voltage...
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Báo cáo hóa học: " Research Article Hardware/Software Codesign in a Compact Ion Mobility Spectrometer Sensor System for Subsurface Contaminant Detection" potx
Ngày tải lên :
22/06/2014, 06:20
... for flexibility in the duration of the extraction and injection states, so that the system can be optimized for differing environmental conditions A single 32-bit control word contains extraction ... fully optimized The high performance FPGA DSP allows for upgrades and higher-order digital filtering techniques that can help improve the signal-to-noise ratio (SNR) and, therefore, the resolving ... into the ionization region of the IMS sensor In the data acquisition stage, the ion gate is cycled open and closed Sin Ming Loo et al RS232 Solenoid drivers FPGA A/D Gate controller High voltage...
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12 CRITICAL STRATEGIES FOR EFFECTIVE EMAIL COMMUNICATION by Jeff Finkelstein pot
Ngày tải lên :
27/06/2014, 23:20
... Thank you for respecting the author's work ***** Thanks to the whole Customer Paradigm team for helping me hone these strategies into working principles ***** 12 CRITICAL STRATEGIES FOR EFFECTIVE ... followed by supporting material, an attachment or a link to more information on a website Enough said I’ll try to keep this section short ***** RULE # 3: KEEP IT RELEVANT I believe that attention ... done by tomorrow ***** RULE # 8: ALWAYS INCLUDE YOUR CONTACT INFORMATION I can’t tell you how many times I’ve not returned a call promptly because I didn’t have someone’s contact information readily...
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Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx
Ngày tải lên :
08/03/2014, 16:20
... 50 (v/v/v) High performance liquid chromatography Protein separation by HPLC was performed using a reversed phase Vydac Protein C-4 column (250 · 4.6 mm internal diameter, The Separation Group, ... higher than average contribution to the UV optical absorption and protonation of amino groups by electrospray ionization, leading to falsely high estimates of protein loss by these methods (L Zolla, ... estimation of components present in individual subcomplexes obtained by dissociation in a low ionic strength solution Information was collected on the possible supramolecolar organization and...
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Báo cáo y học: "Quasi-MSn identification of flavanone 7-glycoside isomers in Da Chengqi Tang by high performance liquid chromatography-tandem mass spectrometry." ppsx
Ngày tải lên :
13/08/2014, 15:21
... microscopic identification method Instruments and conditions High- performance liquid chromatography- diode array detection (HPLC-DAD) analysis was performed on a liquid chromatography system (Agilent ... electrospray mass spectrometry; LC-ESI/MS: liquid chromatography- electrospray mass spectrometry; HPLC-DAD: highperformance liquid chromatography- diode array detection; MS/MS: tandem mass spectrometry; RDA: ... The ions at m/z 227 would undergo further loss of CO at the position carbon 4' of B-ring and generate ion at m/z 199 A retrocyclisation fragmentation involving the and bonds was proposed to form...
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Báo cáo y học: "Comparison of raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry" pptx
Ngày tải lên :
13/08/2014, 15:21
... limits of detection, evaluated by a signal-tonoise ratio of about 3:1 for the standard solution, were 0.575 μg/ml, 0.343 μg/ml and 0.523 μg/ml for compounds 1, and respectively The correlation coefficients ... chromatographic peaks and calculated the correlation coefficients for similarity of the chromatograms Results and discussion Optimization and validation of HPLC conditions Figure The change of relative contents ... flame ionization and mass spectrometric detection J Chromatogr A 2008, 1200:43-48 34 Li BY, Hu Y, Liang YZ, Huang LF, Xu CJ, Xie PS: Spectral correlative chromatography and its application to analysis...
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studying histamine in seafood products by high performance liquid chromatography
Ngày tải lên :
06/10/2015, 13:05
... determined by means of standard chromatographic techniques such as thin layer chromatography, gas chromatography, capillary electrophoresis, flow injection analysis, and high performance liquid chromatography ... the highest histamine concentration was from 0.6M perchloric acid for samples analysed by dansylation procedure The samples extracted by trichloroacetic acid 6% and analysed by benzoylation procedure ... (B): chromatography derived by benzoylation procedure; (C) and (D): chromatography derived by dansylation procedure Figure 6: Mean of histamine concentrations in tuna samples determined by dansylation...
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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc
Ngày tải lên :
07/03/2014, 21:20
... performed the preliminary analysis and detection for all the effluent from CPC240 constantly The chromatogram was recorded with Agilent ChemStation Fractions were collected by a CF-1 fraction ... essential oil of Curcuma wenyujin and (B) Fraction 1, (C) Fraction 2, (D) Fraction 3, (E) Fraction 4, (F) Fraction and (G) Fraction from HPCPC separation 1, curdione; 2, curcumol; 3, germacrone; 4, curzerene; ... gives a higher retention level of the stationary phase, improves the peak resolution and also requires a longer separation time Using a lower revolution speed reduces the volume of the stationary...
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Humic acid removal from aqeous solution by hybrid eletrodialysis ion exchange
Ngày tải lên :
22/10/2015, 21:14
... ion exchange resin is used and an anion exchange resin is arranged besides the cation ion exchange membrane and the cation exchange resin is arranged besides the anion ion exchange membrane Performance ... Co-ions Cation exchange resin (-) Cations (+) Anions (-) Anion exchange resin (+) Anions (-) Cations (+) An ion exchange process can be described as a reaction Suppose that the ion exchange resin ... solution The composition of the two main types of ion exchange resin can be summarized in Table 2-1 Table 2-1 Composition of the resins Type of ion exchange Fixed charges Counter-ions Co-ions Cation...
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High-Performance Thin-Layer Chromatography(HPTLC)
Ngày tải lên :
06/10/2016, 09:26
... evaluation because of the possibility of sequential detection by complementary techniques, postchromatographic derivatization for identification and quantification, archiving a separation for evaluation ... For the purpose of investigation, atmospheric pressure-matrix-assisted laser desorption/ionization is chosen for a certain applications depending on the analytes Combination of laser desorption ... and, though higher numbers are possible, they will lead to long separation times When the development length is optimized, the separation performance of conventional and high- performance TLC...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
Ngày tải lên :
07/03/2014, 12:20
... type information, the protein ligand interaction can be probed by [15N]-HSQC FEBS Journal 273 (2006) 4154–4159 ª 2006 The Authors Journal compilation ª 2006 FEBS 4157 15 N-labelled proteins by cell-free ... production of uniformly [15N ⁄ 13C]labelled samples for in-depth NMR analysis Much information can be gleaned already from a single selectively labelled sample For example, binding interactions ... yields in vivo are also suitable for efficient production by cell-free synthesis In order to compensate for the increased effort and expense required for the production and selective isotope labelling...
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Báo cáo y học: "A novel method for purifying bluetongue virus with high purity by co-immunoprecipitation with agarose protein A" ppsx
Ngày tải lên :
12/08/2014, 04:20
... everyday for days And TCID50 was calculated using Karber formula Purification of BTV-10 by Reverse Co-immunoprecipitation with Agarose Protein A Acknowledgements This study was supported by National ... just BTV virions and small amount of microorganisms left And the final step was the condensation of the virus solution by Polyethylene Glycol (PEG-20000) and sterilization filtration by 0.1 μ membrane ... photographed by digital camera and the virions were marketed by arrows The two-layer structure of BTV virion was perfectly shown in picture A Picture B was photographed by film and the virions were...
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NOVEL RESTORATION TECHNOLOGY FOR PUZRIFICATION OF WATER QUALITY IN CLOSED WATER BODY BY HIGH CONCENTRATED OXYGEN WATER
Ngày tải lên :
05/09/2013, 08:40
... E l p se d ti e (d a y) a m Table Operational conditions of Vial experiment condition of experiment The water of bottom layer (after the treatment by high concentrated oxygen dissolver)+soil ... of oxygen In this way, the oxygen accounted for 30 % of the gas supplied to the high concentrated oxygen dissolver, therefore, DO concentration in the high concentrated oxygen water was much lower ... production of high concentrated oxygen water was available by using the high rate, high efficiency oxygen dissolver The 2.3 L lab scale apparatus could make 0.015 m3/min (22 m3/d) of high concentrated...
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Tài liệu IPTV Guide Practical Deployment Strategies for Network Operators ppt
Ngày tải lên :
24/01/2014, 11:20
... ensure reliability and performance for applications ranging from HD mobile production to post-production PPP1248-E90-HN with PPP-15-CHAS-KIT EDAC 90-pin Chassis with 1.5 Designation Strips The UniPatch® ... standard ADC's high performance copper riser and plenum cable for highly automated manufacturing processes that ensure consistent quality and dependability with superior electrical performance produce ... office ADC also offers fiberdistribution terminals, PON splitter modules, connectors and drop cables, as well as solutions for high- performance interconnection of fiber cables and equipment at...
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Báo cáo khoa học: Hyperthermophilic enzymes ) stability, activity and implementation strategies for high temperature applications pot
Ngày tải lên :
07/03/2014, 05:20
... contamination in food and drug biosynthesis applications; (g) enzyme immobilization may increase heat stability and therefore, improve biocatalyst performance; and (h) protein engineering by rational ... utilizing enzymes for biotechnological applications is to ensure that upon immobilization the enzyme remains functional Protein adsorption mechanisms and events The interaction of proteins with surfaces ... adsorption (i.e excess accumulation of protein at the interface compared to the bulk) Physical adsorption is a mild method of immobilization Protein adsorption events are largely directed by interfacial...
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Báo cáo khoa học: TICL – a web tool for network-based interpretation of compound lists inferred by high-throughput metabolomics doc
Ngày tải lên :
16/03/2014, 01:20
... structure transformation patterns for substrate–product pairs (reactant pairs) We can build a global ‘reaction network’ (reactions are nodes, compounds are edges) by connecting edges and reactions that ... Journal compilation ª 2009 FEBS ¨ A V Antonov et al TICL – a tool for interpretation of compound lists Fig Visualization of the inferred network model D3 returned by TICL after submission of 82 compounds ... influenced by the incomplete information currently available for metabolic reactions Another reason might be that the identified markers not necessarily reflect structural metabolic variations, because...
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Báo cáo khoa học: Construction of a novel detection system for protein–protein interactions using yeast G-protein signaling pdf
Ngày tải lên :
16/03/2014, 01:20
... sample buffer for SDS ⁄ PAGE at an A600 of 20 Fractionated cell lysates were prepared by glass bead vortex homogenization for 15 Detection system for protein protein interactions Protein extracts ... In conclusion, we have established a novel detection system based on G -protein signaling for detection of protein protein interactions, using a mutated Gc that lacks membrane-localization ability ... establish a rapid and reliable method for the detection of protein protein interactions using yeast G -protein signaling In our system, protein protein interactions were detected utilizing the knowledge...
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