... N-terminal regions of the a-subunits serves as catalytic domains, and the C-terminal regions of the a-subunits and the b-subunits act as regulatory domains [10,11] The regulatory domains of AK contain ... composed of a catalytic domain from BsAKII and regulatory domains (a regulatory domain in the a -subunit, and a b -subunit with the same sequence as the regulatory domain) from TtAK, improved thermostability ... TtAK subunits of SDS ⁄ PAGE in (B) The a-subunits and b-subunits are indicated by a solid line with circles and a dotted line with squares, respectively (E) Densitometric calibration of TtAK subunits...
... b subunits to form dimers composed of two b subunits, of two btes subunits and, of the mixture of b/btes subunits These interactions are weaker than interaction of a subunit with btes or b subunit, ... with the a subunit are the canonical features of the regulatorysubunit of CK2 CK2btes protein interacts with CK2 a subunit in yeast two-hybrid system To examine whether the CK2 a subunit is ... testis-specific isoform of the CK2 regulatorysubunit in normal males The CK2 regulatorysubunit is ubiquitous among eukaryotes, but an amino-acid sequence of different b subunits is far less conservative...
... that PP2A may play important roles in developing eyes, and the functions of PP2A appear to be highly regulated by various regulatory subunits [17] The mRNA isoforms of the PP2A Asubunit and B -subunit ... gene promoters Among the various PP2A genes, we found that the Ppp2r5c (PP2A- B56c), Ppp2r2b (PP2A- B55b; data not shown) and Ppp3cc (protein phosphatase 3, catalytic subunit c; data not shown) genes ... novel regulatorysubunit of protein phosphatase 2A FEBS Lett 460, 462–466 Zolnierowicz S, Csortos C, Bondor J, Verin A, Mumby MC & DePaoli-Roach AA (1994) Diversity in the regulatory B-subunits...
... Catalytic subunit Core regulatorysubunit of Pph3 ⁄ Ppp4c Regulatorysubunit of Pph3 ⁄ Ppp4c Core regulatorysubunit of Ppp4c Catalytic subunit Catalytic subunit Catalytic subunit Common regulatorysubunit ... Ppp4 and Pph3 subunit structures may thus resemble those of PP2A and Pph21 ⁄ Pph22, where a core regulatorysubunit forms a complex with the catalytic subunit to which a third variable subunit may ... the identification of two regulatory subunits of Ppp4c that not interact with the closely related PP2Ac, namely R1 (105 kDa) [15] and R2 (55 kDa) [16] These regulatory subunits form independent...
... T cells from 21SHL transgenicmice and 21SHL-CL transgenicmice were impaired in their proliferative response to CD3e antibody stimulation to the same extent as in WASP15 transgenic T cells [24] ... anti-WASP scFv transgenic T and B cells, and lymphoid development in anti-WASP scFv transgenicmice (A) T-cell proliferation Splenic T cells from anti-WASP scFv 21SHL transgenic, 21SHL-CL transgenic, ... of at least three male mice for each analysis at the age of weeks purified T cells from spleens of wild-type, WASP15 transgenic, 21SHL transgenic and 21SHL-CL transgenicmice were stimulated with...
... neurons of transgenic HD mice Experimental procedures Animals Two transgenic HD mice colonies were established and maintained by crossing hemizygous R6/2 or R6/1 males with CBAxC57BL/6 females Mice ... striatum of wild-type mice and 3-week-old R6/1 and R6/2 transgenic HD mice (Fig 1A) The CB1-specific hybridization signal was decreased in the lateral striatum of older transgenic HD mice There was no ... derived from the striatum of wild-type and HD transgenicmice Striatal RNA was extracted from 3-, 5-, 6-, and 12 week-old wildtype and R6/1 transgenic HD mice (n ¼ per age and genotype) Three gene-specific...
... the cTnTR141W transgenicmice and CYP2E1 transgenicmice beginning at months of age, with respective death rates of 17.5% in cTnTR141W transgenicmice and 13.75% in CYP2E1 transgenicmice being ... also triggered in CYP2E1 transgenicmice and cTnTR141W transgenic mice, and the apoptosis of myocytes was increased in CYP2E1 transgenicmice and cTnTR141W transgenicmice (Fig 6), suggesting ... WT, cTnTR141W, CYP2E1(#26) and CYP2E1(#36) transgenicmice (Fig 3A and Table 1) The cTnTR141W transgenicmice and the two lines of CYP2E1 transgenicmice exhibited the DCM phenotype (i.e chamber...
... tg-ArcSwe mice (data not shown), consistent with the observed difference in escape latency Representative swim paths of nontransgenic and transgenicmice are shown in Fig 4B Nontransgenic mice clearly ... FEBS 999 Amyloid-b protofibrils in transgenicmice A A Lord et al Fig Spatial learning and memory in tg-ArcSwe miceTransgenic (tg-ArcSwe) and nontransgenic (non-tg) mice were tested in the Morris ... procedures Histology and image analysis Transgenicmice APP transgenicmice with the Swedish (K670N, M671L) and Arctic (E693G) mutations (tg-ArcSwe mice) [20], non- 1002 Mice were anesthetized with 0.4...
... and the transgenic N171-82Q mice (B–D) Measured (circle for control, triangle for transgenic N171-82Q mice) and simulated (solid line for control, dashed line for transgenic N171-82Q mice) curves ... the transgenic HD mice (Table 2), whereas no change was detected in the unaffected regions (data not shown) In the 3-NP mice, the activity of HK had increased more than in the transgenicmice ... mgÆkg)1 into CFLP mice (twice a day) Mice from the same strain received NaCl ⁄ Pi vehicle and were used as a control Mice were sacrificed after the seventh 3-NP injection Transgenicmice expressing...
... its biological properties PKA regulatory subunits control the release of catalytic subunits from the inactive tetramer complex upon binding of cAMP to the regulatory subunit- dimer Thus, we examined ... they contained 1500 bp DNA, 1120 bp of which encoded for the C-terminal portion of the RIIa regulatorysubunit of PKA No hepatic cell line expressing HIP/PAP was available Thus we have established ... HIP/PAP and RIIa were further analyzed using a fractionation method (Fig 1B) The regulatory RIIa and the catalytic Ca subunits of PKA were detected in the 100 000 g ultracentrifugation pellet and...
... days Results obtained with transgenic (n ¼ 11) and control (n ¼ 11) mice are shown in black and gray, respectively 150 s ± 16 versus 146 s ± 18 for transgenic and control mice, respectively) Similarly, ... recombination pattern in the transgenic mouse line, by analyzing one transgenic line in detail (BAC-DATiCre line), and crossing this line with the R26R reporter line [9] In transgenic animals carrying ... populations Efforts have recently been made to generate transgenicmice expressing the recombinase in restricted neuronal populations [8] We generated a transgenic mouse line [bacterial artificial chromosome...
... in all homologs) q FEBS 2001 PKA regulatorysubunit from T brucei (Eur J Biochem 268) 6201 Fig Sequence comparison of cNMP-binding domains of PKA regulatory subunits and of protein kinase G cNMP-binding ... further difference between TbRSU and the PKA regulatory subunits from other eukaryotes is seen in Val319 All cAMP-binding domains of the regulatory subunits carry an alanine residue at this position, ... family of regulatory enzymes Annu Rev Biochem 59, 971 –1005 19 Su, Y., Dostmann, W.R.G., Herberg, F.W., Durick, K., Xuong, N.H., Ten Eyck, L., Taylor, S.S & Varughese, K.I (1995) Regulatory subunit...
... of month old APP [V717I] transgenic mice, while absent in non-transgenics (data not shown) and most significantly increased in the brain of old APP [V717I] transgenicmice at 16 months (Figure ... cortical GFAP-immunostaining was practically absent in non -transgenic control mice at months (data not shown), whereas APP [V717I] transgenicmice had randomly distributed foci of astrocytes strongly ... Methods Animals Transgenicmice expressing APP [V717I] under the mouse thy1 gene promoter in the FVB/N genetic background [6] aged and 16 months were used in this study with nontransgenic mice of the...
... affinity interactions Experimental design Transgenicmice Tg2576 APP mice [2]) were acquired from the breeding colonies at the University of South Florida Multiple mice were housed together whenever ... (untreated) hippocampal regions of 20 mo old APP transgenicmice Panels A, C, and E show total Aβ staining in right hippocampal regions of 20 mo old APP transgenicmice receiving intracranial injection ... hippocampal regions of 20 mo old APP transgenicmice Panels A, C, and E show total thioflavine S staining in right hippocampal regions of 20 mo old APP transgenicmice receiving intracranial injection...
... parallel Materials and methods Transgenic animals The heterozygous Tg197 transgenic mouse was generated and described previously by our research group [3] Briefly, Tg197 mice carry a human TNF transgene ... weeks of age all mice were killed and the hind ankle joints were removed for histology Histologic processing and scoring of joints Ankle joints were removed from the transgenicmice and were fixed ... only 11% of the joints of the mice treated with low dose diacerein and none of the joints of mice treated with dexamethasone or anti-TNF had a similar score, whereas in mice treated with methotrexate...
... between young and aged mice Cartilage in young mice treated with anti-TNF-α was significantly improved relative to saline-treated and baseline mice By comparison, treatment of aged mice with anti-TNF-α ... in both young and aged mice, and a significant reduction in cartilage degradation in comparison with baseline was maintained in young mice Cartilage degradation in aged mice treated with antiTNF-α ... anti-TNF-α monoclonal antibodies reverses established arthritis in Tg197 mice (a) Young mice (at to weeks of age) or (b) aged mice (at 27 to 28 weeks of age) with established arthritis were randomized...
... macrophages from HLA-DR1 transgenicmice for antigen presentation of both the glycosylated and non-glycosylated forms of the arthritogenic CII259– 273 epitope to CD4 T cells Treatment of mice with an inhibitor ... chromatography Animals In all experiments described in this study we used previously reported micetransgenic for HLA-DR1 on a major histocompatibility complex (MHC) class II-deficient background ... glycosylated and non-glycosylated arthritogenic epitope CII259–273 generated from HLA-DR1 -transgenic mice [27], which allowed us to test the effect of post-translational modification on uptake...
... mice were to weeks old; wild type mice and the recipient mice, both HCV transgenic and non -transgenic mice, were to months old The establishment and characterization of these HCV transgenicmice ... immunized mice into HCV transgenicmice Our results showed that the carboxyfluorescein succinimidyl ester (CFSE)-labeled T cells from HCV immunized mice homed to the liver of HCV transgenic mice, ... non -transgenic recipient mouse groups when they received transfers from nonimmunized donors In contrast to wild-type mice, donor cells from immunized mice homed to the liver of transgenic mice...
... shuttle vector [18] 2.2 Generation of transgenicmice Isolation and micro-injection of the modified BAC insert was performed as previously described [15] Transgenicmice were identified both by Southern ... in transgenicmice [9] Expression of this gene was assessed by RT-PCR in the three transgenic lines obtained with the modified BAC It revealed that the 244 S Soulier et al Figure Detection of transgenic ... transgenicmice and CAT expression analysis A: Detection of transgenicmice by Southern analysis of PstI-digested genomic DNA The origin of the genomic DNAs are indicated on the top lane N.Tg.: non-transgenic...
... Additionaltumors in differentiallymice micehyperplastic mammary at thehere forMMTV-Wnt- 1transgenic in MMTV-Wnt-1 micemicetransgenic Filetransgenic/Pten three-fold transgenic/ Ptentissues than fromMMTV-Wnt-1 ... MMTV-Wnt-1 and MMTV-Neu transgenicmice MMTV, mouse mammary tumor virus; NeuT, mammary tumors from MMTV-Neu transgenic mice; WntT, mammary tumors from MMTV-Wnt-1 transgenicmice reference RNAs, ... glands from nontransgenic mice and in hyperplastic mammary glands from MMTV-Wnt-1 transgenic mice, but was readily and widely detected in the tumor samples from MMTV-Wnt-1 transgenicmice (Figure...