potential enzyme activity—kinetic approach
Báo cáo khoa học: A kinetic approach to the dependence of dissimilatory metal reduction by Shewanella oneidensis MR-1 on the outer membrane cytochromes c OmcA and OmcB potx
... the reduction potential and the topology and accessibility of the presented metal play crucial roles in terms of binding efficiencies and subsequent reduction by the appropriate enzyme The identification ... bacterial dissimilatory metal reduction in controlling the fate and transport of metals and their potential for remediation purposes are well recognized, the terminal reductases involved are not ... reduction of a variety of metals, i.e Fe(III), V(V), U(VI), and Se(VI) A ‘whole cell’ kinetics approach was used to determine the kinetic parameters for OmcA- and OmcB-dependent chelated Fe(III)...
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Báo cáo Y học: Restoring enzyme activity in nonfunctional low erucic acid Brassica napus fatty acid elongase 1 by a single amino acid substitution pdf
... condensing enzymes is limited due to the fact that these enzymes are membrane-bound, and as such are inherently more difficult to characterize biochemically than soluble condensing enzymes Recently, ... condensing enzymes Their results have shown that cysteine 223 is essential for FAE1 KCS activity, and that it seems to have a similar role to the active-site cysteines present in other condensing enzymes ... FAE1 condensing enzyme activity Here we report and discuss the results of analyses of heterologous expression in yeast and site-directed mutation of Brassica FAE1 condensing enzymes MATERIALS...
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Báo cáo khoa học: Peptides that bind the HIV-1 integrase and modulate its enzymatic activity – kinetic studies and mode of action pptx
... important to identify new targets and, at the same time, to develop new approaches for the design of anti-HIV therapy A promising approach is the use of peptides for inhibition or activation of certain ... block different stages of the HIV-1 life cycle, such as entry into cells [5,6], or inhibit viral enzymes such as the reverse transcriptase [712] and protease [1318] A major problem with the currently ... viral cDNA into the host chromosomal DNA, a step that is crucial for the virus life cycle [35] This enzyme has no human homolog, so it is an ideal target for developing anti-HIV drugs [36,37] The...
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Báo cáo khoa học: Identification of preferred substrate sequences for transglutaminase 1 – development of a novel peptide that can efficiently detect cross-linking enzyme activity in the skin pot
... transglutaminase (TG2) – a wound response enzyme Front Biosci 11, 867–882 Kim SY, Chung S-I & Steinert PM (1995) Highly active soluble processed forms of the transglutaminase enzyme in epidermal keratinocytes ... erythroderma and lamellar ichthyosis [21–25] Thus, TGase appears to be an essential and non-redundant enzyme for CE formation in the epidermis under functional regulation [26] Generally, TGases display ... insight into the substrate specificity of TGases, and expand the range of application using the enzyme reactions [35] In the current study, we used this system to identify preferred TGase 1-specific...
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Báo cáo khoa học: Human salivary a-amylase Trp58 situated at subsite )2 is critical for enzyme activity potx
... reaction of the W58L enzyme with CNP-G4 As a result of increased transglycosylation activity of this enzyme significant amounts of CNP-G5, CNP-G6 and CNP-G7 are produced The wild-type enzyme did not ... enzymes based on Western blotting, enzymes with greater than 99% purity were obtained at approximately mgÆL)1 of the culture medium The mass and purity of the enzymes were confirmed by mass spectral ... acceleration potential Samples were analyzed in delayed extraction linear mode, calibrated externally with bovine serum albumin (Sigma Chemical Co.) All spectra were the result of averaging 200 shots Enzyme...
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Báo cáo khoa học: The C-terminal region of the proprotein convertase 1⁄ 3 (PC1⁄ 3) exerts a bimodal regulation of the enzyme activity in vitro pdf
... Furthermore, control of enzyme activity is accomplished through limited proteolysis of the zymogen molecule A role in controlling the enzymatic activity of PC1 ⁄ has also been ascribed to a potential endogenous ... demonstrated role of the proregion in controlling activation of the enzyme, it appears that the CT-peptide might be implicated in regulating enzyme activity This adds an additional level of complexity ... CT-peptide having an affinity for the enzyme in the same range as the fluorogenic substrate is unlikely to directly compete with substrate at the active site The majority of enzymes sensitive to essential...
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Báo cáo khoa học: Human airway trypsin-like protease induces amphiregulin release through a mechanism involving protease-activated receptor-2-mediated ERK activation and TNF a-converting enzyme activity in airway epithelial cells doc
... measured as follows Anti-AR mAb (clone 12111.333, Genzyme) was used as a capture antibody, and a biotinylated antihuman AR polyclonal antibody (Genzyme) was used as a detection antibody for ELISA ... TAPI-1, a relatively selective metalloprotease inhibitor for tumour necrosis factor a-converting enzyme (TACE), on HAT-induced AR release was determined as it is well known that metalloproteases, ... steps of the pathway leading to HATinduced AR production have been analysed using pharmacological enzyme inhibitors The following results suggest the involvement of PAR-2-mediated activation of...
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Báo cáo y học: " Hyperglycemia alters enzyme activity and cell number in spinal sensory ganglia" ppsx
... as reflected by the endogenous activity of specific homeostatic enzymes, is sensitive to injury and perturbation Therefore, these enzymes were selected as putatively reflective of mitochondrial ... histochemical reaction product in the fifth lumbar dorsal root ganglion from control and diabetic mice Enzyme Enzyme histochemical reaction product in the fifth lumbar dorsal root ganglion from control ... http://www.JBPPNI.com/content/2/1/11 Figure Enzyme histochemical reaction product in the dorsal horn of the fifth lumbar spinal cord from control and diabetic mice Enzyme histochemical reaction product...
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Báo cáo y học: "Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes" pptx
... of enzyme activity is delayed compared to the peak of transcript level by an interval that varies from enzyme to enzyme Two further aspects of the data highlight that transcript levels and enzyme ... transcript levels for 82 genes, which encode the 23 enzymes analyzed in [4] The number of genes is larger than the number of enzymes because many enzymes are encoded by small gene families For each ... and/or variations in enzyme activities Gibon et al R76.9 comment enzyme activities became apparent, while the changes in metabolites became only slightly larger Only data for enzyme activities and...
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Báo cáo Y học: Domain V of m-calpain shows the potential to form an oblique-orientated a-helix, which may modulate the enzyme’s activity via interactions with anionic lipid potx
... of enzyme activity of a number of proteins [23] A number of studies have suggested that interaction of m-calpain domain V with lipid or membranes may also be involved in the modulation of the enzyme s ... showed that a C-terminal segment of domain V, G17TAMRILGG, is required for lipid interaction of the enzyme [24] More recent investigations, using peptides homologous to various regions of m-calpain’s ... activity [46,48] It is clear from our theoretical analyses that the GTAMRILGGVI segment has the potential to form an a-helix with strong structural similarities to the obliqueorientated a-helix...
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Tài liệu Understanding Potential Changes to the Veterans Equitable Resource Allocation (VERA) System - A Regression-Based Approach ppt
... using either the single- or two-equation approach. 1 As will be described in Chapter Three, this single-equation approach performed as well as the two-equation approach and is much easier to estimate ... Congress Cataloging-in-Publication Data Understanding potential changes to the Veterans Equitable Resource Allocation System (VERA) : a regression-based approach / Jeffrey Wasserman [et al.] p cm “MG-163.” ... approach was similar to that of Phase II, with several important differences: • We used more recent data sets to estimate costs and to simulate VISN allocations • We simplified our modeling approach...
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Tài liệu Báo cáo khóa học: UDPgalactose 4-epimerase from Saccharomyces cerevisiae A bifunctional enzyme with aldose 1-epimerase activity docx
... a-D-galactose) during an enzyme catalyzed mutarotation reaction [10] a-D-Glucose (65 mM) was dissolved in mM Tris HCl, pH 7.4 buffer containing mM EDTA, immediately before addition of the enzyme The solution ... purified enzyme (Fig 3) A similar kinetics was observed when a-D-galactose was used as the substrate Additionally, a coupled assay method using b-D-glucose dehydrogenase as the coupling enzyme ... as + D-glucose or L-arabinose, the NAD bound form of the enzyme is slowly but irreversibly reduced to NADH The formation of NADH on the enzyme surface is accompanied by progressive enhancement...
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Continuing Challenges and Potential for Collaborative Approaches to Education Reform pot
... successes of this approach to school improvement During this period, RAND tracked the sites’ progress toward CERI’s goals and xi xii Continuing Challenges and Potential for Collaborative Approaches ... improvement? xiv Continuing Challenges and Potential for Collaborative Approaches To help answer these research questions, we chose a replicated case-study approach, viewing each collaborative and ... Purpose and Approach Remainder of the Monograph CHAPTER TWO Approach, Concepts,...
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Báo cáo khoa học: Kinetic properties of catecholoxidase activity of tarantula hemocyanin pot
... number and [ET] is the enzyme concentration Substrate inhibition was tted according to the simplest model, assuming that a second substrate molecule can bind 1526 to the enzymesubstrate complex ... physiological function [2,3] The activation of hemocyanin by either antimicrobial peptides or clotting enzyme, as observed in the horseshoe crab Tachypleus tridentatus, supports this idea [18,19] Although, ... 1) As an equilibrium condition (i.e constant activity) is a prerequisite for the measurement of enzyme kinetics, the conditions with constant activity were established This was accomplished by...
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Báo cáo khoa học: The pH dependence of kinetic isotope effects in monoamine oxidase A indicates stabilization of the neutral amine in the enzyme–substrate complex ppt
... attributed to aggregation of the detergent solubilized enzyme at the high concentrations required for stopped-flow assays To minimize this potential effect, the same concentration of MAO A was ... pH dependence in MAO A ionizations may be due to the substrate rather than to groups on the enzyme A potential way to identify substrate ionizations is to perturb the substrate pKa (e.g by deuteration) ... binds to the enzyme, with subsequent substrate deprotonation required for catalysis to proceed Therefore, the macroscopic ionization in the ES complex was assigned to a group on the enzyme, rather...
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Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf
... lysosomal enzyme widely distributed in almost all mammalian cells [5,9,11,12] Studies with CatE-deficient and CatD-deficient mice have provided additional evidence of the association of these enzymes ... properties This approach distinguishes between the activities of the enzymatically similar proteinases, CatE and CatD, and can therefore be used to investigate the involvement of these enzymes in ... localization of CatE and CatD in different cell types, but there are few reports on the activity of these enzymes in organelles relevant to antigen-processing [5,30] Previous reports have described highly...
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Báo cáo khoa học: Spectroscopic and kinetic properties of the horseradish peroxidase mutant T171S Evidence for selective effects on the reduced state of the enzyme potx
... lignin peroxidase [3] This region of the structure has particular relevance in both enzymes because of its potential to provide structural coupling between the proximal Ca2+ ion and the residues ... have proven to be particularly intriguing and specic to the reduced state of the enzyme The Fe(II) state of the enzyme has features in common with both the Phe221Met mutant and Ca-depleted proteins ... wild-type enzyme at pH 6.8 [13] and indicative of a 5-c HS haem species However, the low frequency region of the RR spectrum of the T171S variant differs markedly from that of the parent enzyme...
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Team building approach: Unlocking true potential of team members
... Create a “bias for action” 20 Basics - SWOT Analysis Critical to a team understanding itself and potential Strengths Weaknesses Opportunities Threats How can I avoid team disasters? Every ... A concept that had previously failed Only one chance for success Selected people with potential , but weren’t a team (yet) Prioritized how our field sales people would judge us Didn’t ... “Group Development” Bruce Truckman’s Five Stages of Group Development Forming – scope of task and approach Everyone is nice and polite Storming – dealing with group conflict Real issue now being...
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Internal Audit 2012*: A study examining the future of internal auditing and the potential decline of a controls-centric approach docx
... other, potentially more effective, players in the risk management discipline When discussing these possibilities, a number of CAEs interviewed for this report said they could foresee potential ... need to identify and develop potential leaders, advised the audit leader of a global consumer products company “Ideally,” he said, “internal audit will train high -potential employees in key areas ... in 2012, internal auditors need to move beyond a static, cyclical audit approach and adopt a continuous, comprehensive approach to audit and risk assessment—one that optimizes the use of technology...
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