polarized targeting in epithelial cells
Báo cáo y học: "Nipah virus infection and glycoprotein targeting in endothelial cells" ppsx
... endothelial cells whereas only tyrosine 525 is involved in targeting in epithelial cells Neither the di-tyrosine nor the di-leucine motif in the cytoplasmic tail of the G protein influenced G ... Glycoprotein distribution in NiV-infected polarized cells Glycoprotein distribution in polarized cells upon single expression Distribution of glycoproteins with mutations in potential cytoplasmic sorting ... cytoplasmic tail (Y525RSL; [11-15]) Interestingly, the same motif is also involved in basolateral sorting of the F protein in polarized epithelial cells In a very recent study in which we attempted to elucidate...
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Báo cáo y học: " Antioxidant components of naturally-occurring oils exhibit marked anti-inflammatory activity in epithelial cells of the human upper respiratory system" pdf
... aqueous control preparation containing 0.75% peppermint oil as a masking agent was administered in a single-blinded manner as a single 50 μl application in each nostril using a metered spray applicator ... early as hr following exposure [47] suggesting very rapid initiation of pro-inflammatory signaling In the present study, the effectiveness of the oil in reducing inflammation following exposure to ... occasional mononuclear and infrequent eosinophilic cells This increase in inflammatory response was statistically significant within this treatment group In the same subjects undergoing pretreatment with...
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Báo cáo y học: " Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells" pdf
... Cytokine and chemokine gene expression in type I-like alveolar epithelial cells after influenza virus infection Figure Cytokine and chemokine gene expression in type I-like alveolar epithelial cells ... released from the basolateral domain of polarized cells, the symmetrical or asymmetrical binding, internalization and budding of virus from polarized epithelial cells in culture therefore would have ... previous finding the differential induction of proinflammatory cytokine by influenza H5N1 virus in alveolar epithelial cells [10] We now have thus confirmed that these differences also apply in polarized...
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Tight junctions and adherens junctions quantifying adhesion and role in mechanotransduction in epithelial cells
... subjected to mechanical strain with each heart beat, epithelial cells lining the alveoli in the lungs are stretched during inspiration, and epithelial cells lining the gastrointestinal tract and renal ... stimuli The influence of external stimuli, in particular mechanical stimuli, on endothelial cells lining blood vessels and epithelial cells lining the respiratory, gastrointestinal and urinary tract ... tail interacts with β-catenin which interacts with α-catenin α-catenin in turn links it to the 13 actin cytoskeleton[46] There are pockets in between the extracellular domains for the binding...
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Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf
... enrichment in iNOS activity within the particulate fraction of epithelial cells Apical distribution of iNOS in intestinal epithelial cells To get more insight into the localization of iNOS in intestinal ... to apical domains of intestinal epithelial cells Particulate fraction association of iNOS in vivo In order to determine the distribution of iNOS in intestinal epithelial cells in vivo, experiments ... undetected in control cells, reached maximal levels within 10 h of cytokine exposure and then remained stable during the following h [37] To investigate iNOS induction in polarized epithelial cells, ...
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Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx
... may occur during the initial h window of infection We infected RPE cells with K26GFP [27], and stained cells for F-actin, using phalloidin at 30 and h postinfection, and examined the cells under ... entry into RPE cells V Tiwari et al Fig Role of nectin-1 during HSV-1 entry into RPE cells (A) Antibody against nectin-1 significantly inhibits HSV-1 entry into cultured RPE cells Cells (indicated) ... HSV-1 infection of all target cells, including RPE cells, our study provides new starting points for understanding viral pathogenesis in the retina and advancing novel therapies to control retinal...
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Tài liệu Báo cáo khoa học: Transcriptional upregulation of inflammatory cytokines in human intestinal epithelial cells following Vibrio cholerae infection pptx
... release of cytokines upon V cholerae infection in intestinal epithelial cells Reports have shown the induction of IL-8 in intestinal epithelial cells upon V cholerae infection [11–13] The induction ... Cytokine response in infected epithelial cells A Bandyopadhaya et al Fig Induction of cytokine expression in intestinal epithelial cell lines by V cholerae (A) Int407 and T84 cells were infected ... even upon infection (Table 1) This indicates that cytokines produced by intestinal epithelial cells are likely to play a more important role in initiating and regulating the innate mucosal in ammatory...
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Tài liệu Báo cáo khoa học: Caveolin-1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells docx
... immunofluorescence of Caveolin-1 and P2X7 in alveolar epithelial E10 cells To examine the possible colocalization of the purinergic P2X7 receptor with Caveolin-1 in alveolar epithelial E10 cells, we analyzed ... subconfluent cells was determined Figure 5A shows a Caveolin-1 staining pattern at the cell surface, confirming the known caveolar location of Caveolin-1 In addition, intracellularly distinguishable ... receptors in nonraft microdomains of submandibular gland cells are solely involved in ion channel activity, and that receptors present in lipid rafts may regulate the activities of proteins included in...
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Báo cáo Y học: RING finger, B-box, and coiled-coil (RBCC) protein expression in branchial epithelial cells of Japanese eel, Anguilla japonica pot
... (Sigma) containing 10% (v/v) fetal bovine serum and 100 unitsÆmL)1 penicillin The cells were maintained in humidified atmosphere with 5% (v/v) CO2 at 37 °C The eRBCC cDNA was introduced into the pcDNA3 ... determine the type of cells expressing eRBCC Serial sections were stained with anti-eRBCC and anti-(Na+,K+-ATPase) In freshwater specimens, anti-eRBCC immunostaining was observed mainly in epithelial ... Conserved cysteine/ histidine residues in the RING finger domain and B box domain are circled The potential coiled-coil and B30.2 domain are underlined Potential polyadenylation site in the 3¢-untranslated...
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Báo cáo khoa học: Transport of the phosphonodipeptide alafosfalin by the H+/peptide cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells doc
... for Caco-2 cells, 20 and 50 lM for SKPT cells) in the presence of increasing amounts of alafosfalin The linear, nonmediated transport components, i.e simple diffusion plus tracer binding, of 19.2% ... alafosfalin; in the presence of alafosfalin the Kt value of Gly-Sar uptake in Caco-2 cells was 1160 lM compared to 580 lM in the absence of the inhibiting phosphonodipeptide The same alafosfalin effect ... was observed in SKPT cells where the Vmax values were 2.2 ± 0.1 nmolÆ(10 min)1)Æ(mg protein))1 in the absence and 2.0 ± 0.2 nmolÆ(10 min)1)Æ(mg protein))1 in the presence of alafosfalin, but the...
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Báo cáo khoa học: Expression of heme oxygenase-1 is repressed by interferon-c and induced by hypoxia in human retinal pigment epithelial cells pot
... RPE cell lines In contrast to interferon-c, hypoxia induced the expression levels of HO-1 mRNA in D407 cells and exerted no or only marginal effects in ARPE-19 cells (Fig 2) In D407 RPE cells, the ... ARPE-19 cells, indicating that Bach1 is not a sole determinant for HO-1 mRNA expression in ARPE-19 cells Likewise, hypoxia induced expression of HO-1 and Bach1 mRNAs in D407 cells, indicating that ... of interferon-induced inhibition of Toxoplasma gondii replication in human retinal pigment epithelial cells Infect Immun 64, 4188–4196 Arden, G.B (2001) The absence of diabetic retinopathy in...
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Báo cáo khoa học: Human airway trypsin-like protease induces amphiregulin release through a mechanism involving protease-activated receptor-2-mediated ERK activation and TNF a-converting enzyme activity in airway epithelial cells doc
... performed NCI-H292 cells were nonenzymatically collected by incubation in NaCl ⁄ Pi containing mm EDTA for at 37 °C After washing in NaCl ⁄ Pi containing 1% FBS, the cells were stained with anti-TACE ... subsequent AR protein production; however, an increase in AR protein release in NCIH292 cells was only observed by HAT stimulation Further, the increase in the AR content in PAR-2 AP-treated cells did ... ERK activation through PAR-2 induces AR protein production but not protein release into the culture supernatant Since HAT-induced initial ERK activation results in induction of AR gene expression,...
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báo cáo hóa học:" Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson''''s disease" doc
... is found in the RPE cells in our study However, the depolarization-induced DA release is not detected in the cells, indicating that the DA release machinery as seen in most excitable cells is ... brain injection on a stereotaxic frame (Myneurolab, USA) 6-OHDA (6 μg in μl) was dissolved in normal saline containing ascorbic acid (0.2 mg/ml), and injected into the right medial forebrain ... peroxidase-coupled avidin-biotin staining kit (ABC kit, Vector Laboratories, UK) were used For HE staining, the tissue sections were submerged into the hematoxylin solution (0.5% hematoxylin, 5% aluminium ammonium...
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Báo cáo hóa học: " Role of protease-activated receptor-2 on cell death and DNA fragmentation in Helicobacter pylori-infected gastric epithelial cells" ppt
... by inhibition of PAR-2 expression using PAR-2 AS ODN in AGS cells Additionally, inhibition of PAR-2 activity using trypsin inhibitor increased cell death and DNA fragmentation in H pylori-infected ... and JNK1/2 increased by H pylori in AGS cells H pylori did not affect total forms of p38, ERK1/2, and JNK1/2 in AGS cells H pylori-induced activation of MAPK was inhibited in the cells transfected ... pyloriinduced activation of MAPK in gastric epithelial cells H pylori -induced increases in phospho-specific forms of p38, ERK1/2, and JNK1/2 were inhibited by treatment by treatment of SBTI in...
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báo cáo hóa học: " Inhibition of gap junctional Intercellular communication in WB-F344 rat liver epithelial cells by triphenyltin chloride through MAPK and PI3-kinase pathways" pdf
... to be critical in mediating several aspects of PDGF actions in various cells [23,58-62] To explore the potential role of PI3K signaling in the signaling processes involved in TPTC-induced disruption ... rat liver epithelial cells [43] were cultured in D medium supplemented with 5% fetal bovine serum and 1% [v/v] penicillin/streptomycin antibiotic The cells were grown at 37°C in a 5% CO2 incubator ... fluorescent intensity did decrease in FITC stain after treatment with 1.5 ppm of TPTC for 30 was also described in cancer cells [77,78] Alteration in expression of connexins may be involved in the...
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Báo cáo khoa học: "Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells" pptx
... increased the MDA in human [23] In this study, PM2.5 induced more MDA formation than PM10 suggesting that PM2.5 induced more oxidative stress than PM10 In the single strand breakage assay using ... lung epithelial cell, rat type II epithelial origin, was obtained from ATCC (Manassas, USA) and cultured Ham’s F-12 media (Gibco) containing mM L-glutamine supplemented with 0.01 mg/ml bovine ... (Sigma) hydrolyzed in 1% sulfuric acid Hoechst 33258 dye staining The cells were fixed with a 10% formalin phosphate buffer solution (pH 7.4) for minutes at room temperature After washing with distilled...
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Báo cáo y học: "B cell-activating factor of the tumor necrosis factor family (BAFF) is expressed under stimulation by interferon in salivary gland epithelial cells in primary Sjögren''''s syndrome" ppt
... salivary epithelial cells Cells were obtained from minor salivary glands (a–e) and the HSG cell line (f–i) Positive cells staining for cytokeratin (a) and cytokeratin 19 (b) and the absence of staining ... staining with MPO, CD20, CD3, CD45 and smooth muscle actin excluded the possibility of contamination with myeloid cells, B cells, T cells or myoepithelial cells (Figure 2d–h) The HSG cell line ... [49] Interestingly, IFN-α and IFN-γ increased BAFF mRNA and protein levels in epithelial cells both from patients with pSS and from controls but to a higher level in cells from patients Indeed,...
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báo cáo khoa học: " Salivary a-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cells" docx
... precursor cells [34] but may also appear in cultured epithelial cells [35] To distinguish between different populations of cells, double labeling of cellular cytokeratin and vimentin was performed Cells ... of all cells were both, cytokeratin- and vimentin-positive It is known that epithelial cells in culture might express vimentin [34], so that only those cells exclusively stained for vimentin were ... The proportion of epithelial cells in culture was determined by cytokeratin as epithelial cell marker Additionally, expression of vimentin was determined, which is expressed in fibroblasts and...
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Báo cáo y học: "Inflammatory responses in epithelia: endotoxininduced IL-6 secretion and iNOS/NO production are differentially regulated in mouse mammary epithelial cells" pot
... p65 and p50, increased their binding activity in response to ET compared to the basal binding activity in SCp2 cell nuclear protein extracts Figure 3A shows an abrupt increase in ET-induced p65 ... epithelial cells, likely to be the first cells in contact with infecting bacteria of the mammary gland, play an important role in innate immunity and in recruiting immune cells and orchestrating their ... (FBS), insulin (5 μg/ml) and gentamicin (50 μg/ml) at 37°C in a humidified atmosphere (95% Air; 5% CO2) ET-induced inflammation in SCp2 mouse mammary secretory -epithelial cells To assay for the inflammatory...
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