pneumophila infection of transfected cho cells
Báo cáo y học: "TLR2 and TLR4 triggering exerts contrasting effects with regard to HIV-1 infection of human dendritic cells and subsequent virus transfer to CD4+ T cells" docx
... affecting the direct productive infection of IM-MDDCs with HIV-1 since treatment with EFV reduced the level of viral transfer close to that observed for untreated cells Acute infection studies performed ... secretion of other proinflammatory cytokines or recruit other immune cells, thus possibly increasing the number of Page 11 of 16 (page number not for citation purposes) Retrovirology 2009, 6:42 cells ... a final volume of 200 μl Supernatants (half of medium) were harvested either at day 3, and (infection with NL4-3/Balenv) or 5, and 12 (infection with 93TH054) following HIV-1 infection and kept...
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Báo cáo y học: " HIV infection of non-dividing cells: a divisive problem" ppt
... Hatziioannou T, Goff SP: Infection of nondividing cells by Rous sarcoma virus J Virol 2001, 75:9526-31 Katz RA, Greger JG, Darby K, Boimel P, Rall GF, Skalka AM: Transduction of interphase cells by avian ... import of viruses' genome in mammalian cells, let us now focus on retroviruses The ability of lentiviruses to infect terminally differentiated, non-dividing cells has been taken as proof that ... the genome of such viruses is imported into the nucleus The inability of simple retroviruses to infect non-dividing cells on the other hand has been taken as proof that the genome of such viruses...
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Báo cáo y học: " Legionella pneumophila infection induces programmed cell death, caspase activation, and release of high-mobility group box 1 protein in A549 alveolar epithelial cells: inhibition by methyl prednisolone" pot
... substrate of caspase 3, PARP, in 30 20 ∗ ∗ -10 Days after infection MOI MOI 10 MOI 100 MOI 400 Figure effect of L pneumophila on A549 cells Cytotoxic Cytotoxic effect of L pneumophila on A549 cells ... fragmentation of L pneumophila- infected A549 cells We investigated nuclear DNA fragmentation in infected A549 cells as a possible mechanism of L pneumophilainduced cytotoxicity by TUNEL staining A549 cells ... fragmentation of L pneumophila- infected A549 cells detected by the TUNEL method Nuclear DNA fragmentation of L pneumophila- infected A549 cells detected by the TUNEL method MOI doseresponse relationship of...
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Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc
... time of infection (24 h post-transfection), the cells were 44% positive for murine CD40 vs 2.4% background staining of mock -transfected cells (data not shown) At 20 h postinfection, 43.7% of CD40 -transfected ... experiments, SKBR3 cells at 90–95% confluence in a 6-well plate were transfected with lg of pRSV-mCD40 (gift of G Bishop, University of Iowa) using Lipofectamine 2000 (Invitrogen) in the presence of FBS Media ... as determined by fluorescence of uninfected cells Some 43.7% of CD40 transfected cells were determined to be GFP positive vs 12.6% of mock -transfected cells DC (Fig 3B) In contrast, the scrambled...
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Báo cáo hóa học: " Human adenovirus type 19 infection of corneal cells induces p38 MAPK-dependent interleukin-8 expression" potx
... Figure HAdV-19 infection of keratocytes induces activation of p38 MAPK HAdV-19 infection of keratocytes induces activation of p38 MAPK Lysates were prepared 30 after HAdV-19 or mock infection (A) ... by which HAdV-19 infection of the eye induces corneal cells to express spe- HAdV-19 infection activates p38 MAPK To determine whether adenovirus infection induced the activation of p38 MAPK, we ... IL8 expression, we studied the role of p38 MAPK in HAdV19 infection of keratocytes and their subsequent expression of IL-8 Herein, we show that infection of keratocytes with HAdV-19 activates...
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báo cáo khoa học: "Upregulated expression of indoleamine 2, 3-dioxygenase in CHO cells induces apoptosis of competent T cells and increases proportion of Treg cells" pptx
... analysis of IDO protein expression in CHO- IDO cells using anti-IDO antibody In transfected group, CHO cells transfected with IDO expressed the 42 kDa IDO protein, indicating that CHO cells stably transfected ... inhibitor of IDO in co-culture of CD3+T cells and IDO+ CHO cells, the apoptosis could not be induced (only 33.1 ± 4.87% of CD3+T cells were apoptotic) (Figure 2) Page of 10 cells co-cultured with CHO/ EGFP ... expressed by transfected CHO cells possessed functional activity and could metabolize tryptophan (Figure 1D) Effect of IDO+ CHO cells on CD3+T cell apoptosis After 72 h of co-culture of CD3+T cells...
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Báo cáo y học: "Japanese Encephalitis Virus wild strain infection suppresses dendritic cells maturation and function, and causes the expansion of regulatory T cells" doc
... activities to T cells To test whether P3 infection will impair the ability of DCs to activate allogeneic naïve T cells, the direct effect of P3-infected DCs in activation of naïve T cells was analyzed ... the virus-induced alteration of DCs is a likely cause of the immunosuppression found in JEV infection Results JEV P3 infection of DCs in vitro and in vivo The purity of the bmDCs fraction from ... impairment of activating of allogeneic naïve T cells of P3 infected DCs could be involved in the JE development Treg is a subset of CD4+ T-cell with regulatory properties Previous studies on the role of...
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Báo cáo khoa học: " Selective receptor expression restricts Nipah virus infection of endothelial cells" pot
... endothelial cells) [28] and Ea.hy 926 cells derived from human umbilical vein endothelial cells [29] As a control, Vero cells (permissive to NiV infection) and non-permissive HeLa cells were used ... cells were used [30] For infection studies, cells were seeded on coverslips, grown to confluency and subsequently infected with NiV at a multiplicity of infection (MOI) of 0.2 All work with live ... receptor-positive cells; this assay therefore allows testing of cell lines for functional receptor expression independent of NiV replication HeLa cells which not support NiV-mediated fusion were transfected...
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Báo cáo y học: " Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells" pdf
... between the two routes of infection in terms of release of A B Figure enza virus infection experiment Representation of the transwell insert-setup during the influRepresentation of the transwell insert-setup ... whether the apical and basolateral infection route led to qualitative or quantitative differences in the profile of cytokines induced The efficiency of infection of the cells by the apical route was ... basolateral infection of type I-like alveolar epithelial cells, the titers of influenza H5N1 virus at the apical aspect of the cells is significantly more seen with H1N1 infected cells Single...
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Báo cáo y học: "Phosphatidylserine treatment relieves the block to retrovirus infection of cells expressing glycosylated virus receptors" ppt
... staining of NIH 3T3 cells was undertaken as described in Materials and Methods Middle panel: LAPSN(RD114) infection of NIH 3T3/ASCT2 cells Bottom Panel: LAPSN(RD114) infection of NIH 3T3 cells Data ... infection of the NIH 3T3/ASCT2 cells However, infection of NIH 3T3 cells was not detectable until a higher concentration of PS was reached, further support- Here we report that PS treatment of ... amount of PS (200 µM) (Figure 3) The total amount of PS incorporated into the plasma Figure infection PS at a reduced Effects of of NIH 3T3 cells concentration on RD114 vector Effects of PS at...
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Báo cáo y học: " Involvement of claudin-7 in HIV infection of CD4(-) cells" pdf
... CLDN-7158 Effect of CLDN-7 molecules upon infection of 293T cells by HIVNL4-3 Figure Effect of CLDN-7 molecules upon infection of 293T cells by HIVNL4-3 A) HIVNL4-3 Env(-) virus infection of either ... viral infection The susceptibility of these transfected cells to Env(-) HIVNL4-3 infection is significantly increased Compared with non -transfected cells, the numbers of infected cells in the transfected ... that the infection rate of LNCaP cells by HIV was similar to that of HeLa-CD4, suggesting that the infectivity of HIV-1 for some types of CD4(-) cells is high Because 12% HIV infectivity of HeLaCD4...
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Báo cáo y học: " Targeted infection of HIV-1 Env expressing cells by HIV(CD4/CXCR4) vectors reveals a potential new rationale for HIV-1 mediated down-modulation of CD4" doc
... numbers (104 to × 104) of Env- BJAB cells, the parental cells of TF228 As shown in Figure 6, addition of increasing numbers of Env- cells dramatically decreased the number of neomycin resistant ... expressing target cells Env+ and Env- Kinetics of HIV(CD4) particle adhesion to cells Equal amounts of virus particles (ng p24) generated using HDPack1 in HeLa cells were added to HeLa and CHO cells that ... portions of HIV-1 Env appeared to be expressed at slightly lower levels A similar difference was observed by immunofluorescence of transfected cells (Figure 1C) Gentle permeabilization of the fixed cells...
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Identifying key signatures of high productivity from transcriptome and proteome profiles of CHO cells
... FACS profile of the transfected population in Figure 3.7 Two plates each of high fluorescing and low fluorescing cells were sorted Figure 3.7: FACS profile of non -transfected (CHO/ dhfr-) and transfected ... map of pSV2-dhfr-GFP 23 Figure 3.6: Work flow for the construction of pSV2-dhfr-GFP .24 Figure 3.7: FACS profile of non -transfected (CHO/ dhfr-) and transfected (CHO- dhfrGFP) cells ... selective media (a) Cells under normal illumination (b) Fluorescent cells 43 Figure 4.3: FACS profile of CHO/ dhfr- and CHO- dhfr-GFP after weeks in selective media A shift of profile towards...
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Optimal design of PEM fuel cells to generate maximum power: A CFD study
... the influence of the operating parameters on fuel cell performance to investigate the in situ total displacement and degree of the deformation of the polymer membrane of PEM fuel cells The model ... decreases with increasing of the cell operating pressure This is because of the exchange current density of the oxygen reduction reaction increases with increasing of the cell operating pressure ... simulation of the processes under realistic conditions is essential to the development and optimization of fuel cells, improve long-term performance and lifetime, the introduction of cheaper...
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Tài liệu Báo cáo Y học: Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium pptx
... (Fig 2) Lysates of homogenized wild-type cells were spun to yield an organellar pellet consisting of mitochondria and peroxisomes, and a cytosolic supernatant Equal fractions of each of the protein ... signi®cance of this similarity is currently being addressed Demonstration of the cytosolic location of Mls1p in wild-type cells grown on ethanol completes the picture of the extra-peroxisomal location of ... mutant cells often grow to some extent on these plates but transparent zones in the opaque medium around regions of cell growth indicate utilization of the fatty acid substrate [25] Application of...
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Cytoskeleton reorganization mediates alpha beta integrin-associated actions of laminin on proliferation and survival, but not on steroidogenesis of ovine granulosa cells pdf
... legend of Figure (a) and (b): numbers of cells at 144 h of culture; (c): proliferation rates of GC from small follicles at 48 h of culture; (d): pyknotic rates of GC from large follicles at 48 h of ... (percentage of labeled cells) and the pyknotic index (percentage of pyknotic cells) after 48 h of culture This has been shown to allow the be the optimal culture time for the study of the effects of various ... The percentage of the different morphological states of GC (spread cells, spindle-shaped cells, round cells) was established by counting 1000 to 1500 cells per culture well Page of 17 (page number...
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Báo cáo khoa học: Differential post-translational modification of CD63 molecules during maturation of human dendritic cells potx
... immature and mature dendritic cells, the amount of the 34 kDa form of CD63 disappeared gradually during the chase period, concomitantly with the appearance of isoforms of CD63 of 50 and 70 kDa, respectively ... indicate that the 70 kDa isoform of CD63 is a result of post-translational modifications of the 34 kDa isoform that occurred exclusively in mature dendritic cells Characterization of post-translational ... Fig CD63 isoforms in immature and mature dendritic cells (A) Immature dendritic cells, mature dendritic cells (DC) and Mel JuSo cells incubated for 48 h in the absence or presence of IFN-c were...
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Báo cáo khoa học: Pituitary adenylate cyclase-activating polypeptide attenuates streptozotocin-induced apoptotic death of RIN-m5F cells through regulation of Bcl-2 family protein mRNA expression pdf
... released from dead cells, due to the loss of cell membrane integrity observed in both necrotic and apoptotic cells The long-term exposure of RIN-m5F cells to STZ at concentration of > 10 mm induced ... indicated that h of exposure of RIN-m5F cells to STZ induced a rapid and significant elevation in the caspase-3 activity within h, which preceded the loss of cell viability The activation of caspase-3 ... decrease in cell viability, the co-exposure of RIN-m5F cells with some neuropeptides at concentrations of 10)7 m attenuated the cytotoxicity of STZ (Table 1) Of all glucagon-secretin-family peptides...
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Detection of breast cancer cells using targeted magnetic nanoparticles and ultra-sensitive magnetic field sensors potx
... Mexico School of Medicine, MSC07 4025, University of New Mexico, Albuquerque, NM 87131, USA 3Department of Pathology, University of New Mexico School of Medicine, MSC08 46401 University of New ... highest level of cell-associated anti-Her2 antibody-conjugated nanoparticles, MDA-MB-231 cells showed intermediate binding of targeted nanoparticles, and CHO cells showed a low level of nanoparticle ... Department of Cell Biology & Physiology, University of New Mexico School of Medicine, MSC08 4750, University of New Mexico, Albuquerque, NM 87131, USA 2Cancer Research & Treatment Center, University of...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... establishment of embryonic stem (ES) cells, which maintain the ability to form all types of cells in the body, and can differentiate into a variety of cell types in vitro [8] The use of ES cells in ... contrast, TS cells derived from the trophectoderm (TE) of blastocysts contribute only to placenta Although there is an apparent ED between ES cells and EG cells, the ED of TS cells to ES cells (77)...
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