pneumophila in cho fcgammarii cells

Báo cáo khoa học: Okadaic acid induces DNA fragmentation via caspase-3-dependent and caspase-3-independent pathways in Chinese hamster ovary (CHO)-K1 cells pot

Ngày tải lên : 22/03/2014, 21:20

... fragmentation in CHO- K1 cells, z-DEVD-fmk was successful at inhibiting OA-induced DNA fragmentation in caspase-3) ⁄ ) cells (Fig 2A) The difference between CHO- K1 cells and caspase-3) ⁄ ) cells is ... is involved in the mechanism by which OA induces DNA fragmentation in CHO- K1 cells In the case of caspase-3) ⁄ ) cells, DEVDase or DEVDase-like activity may play an important role in inducing ... fragmentation in CHO- K1 and caspase-3)/) cells To establish whether caspase-3 is required for OA-induced DNA fragmentation in CHO- K1 cells, we investigated the differences in OA response between CHO- K1...

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báo cáo khoa học: "Upregulated expression of indoleamine 2, 3-dioxygenase in CHO cells induces apoptosis of competent T cells and increases proportion of Treg cells" pptx

Ngày tải lên : 10/08/2014, 10:21

... kDa IDO protein, indicating that CHO cells stably transfected with IDO could produce IDO protein (D) Analysis of free amino acids in culture supernatant Amino acid level in CHO cells 72 h after ... OKT3 was inhibited in T -cells which were co-cultured with IDO-expressing CHO cells Furthermore, co-cultured of CHO/ IDO with T -cells could inhibit Vav1 mRNA and protein expression in T -cells However, ... obtain G418-resistant transformants CHO cells containing pIRES2-EGFP-IDO were then identified by flow cytometric analysis Detection of IDO gene transcripts in CHO cells and Foxp3 in co-cultured cells...

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Enhancing recombinant protein yield and quality using novel CHO GT cells in high density fed batch cultures 1

Ngày tải lên : 15/09/2015, 17:10

... online feeding strategy for CHO cells producing recombinant human interferon gamma (IFN-γ) and determining the impact on IFN-γ production and glycosylation quality, (2) Examining the signaling ... (CHO GT) cell lines 129 6.3 CHO GT Cells in BC 131 6.3.1 Growth of CHO GT Transfected Pools in BC 131 6.3.2 Proteolytic Activities of Caspases in BC 135 12 6.4 CHO GT Cell Lines in FBC 138 6.5 ... cells of CHO GTO FADD DN pool and CHO GTO FAIM pool in BC 132 Figure 6.5 CHO GTKD cells in BC The viable cell density, viability and percentage of apoptotic cells of CHO GTKD ALG-2 pool and CHO...

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Enhancing recombinant protein yield and quality using novel CHO GT cells in high density fed batch cultures 6c

Ngày tải lên : 15/09/2015, 17:10

... during FBC Sialylation of Recombinant Human IFN-g in CHO GT Cell Lines Figure Speciﬁc productivities of recombinant human IFN-g in CHO GT cells The four CHO GT cells, CHO GTO FADD DN pool ( ), CHO ... cell lines during fed-batch cultures Recombinant IFN-g yields for CHO GTO FADD DN cell line ( ), CHO GTO FAIM cell line ( ), CHO GTKD ALG-2 cell line (~), and CHO GTKD REQUIEM cell line (*) during ... Table I Fold increase/decrease in gene expression in CHO GT and control cell lines Relative increase/decrease in expression (fold change) Cell line CHO GTO Blank pool CHO GTO FAIM pool CHO GTO FAIM...

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Enhancing recombinant protein yield and quality using novel CHO GT cells in high density fed batch cultures b

Ngày tải lên : 15/09/2015, 17:10

... signaling genes in cell culture In this article, we examined the apoptosis signaling pathways induced during BC and FBC of a Chinese Hamster ovary (CHO) cell line producing recombinant human interferon ... signal-regulating kinase Activating transcription factor Baculoviral IAP repeat-containing protein 1/neuronal apoptosis inhibitory protein Baculoviral IAP repeat-containing protein 4/X-linked inhibitor ... repeat-containing protein 4/SURVIVIN Bruton agammaglobulinemia tyrosine kinase Caspase and FADD-like apoptosis regulator, Cﬂar Caspase Gadd153 Direct IAP binding protein with low PI mRNA Death inducing...

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Enhancing recombinant protein yield and quality using novel CHO GT cells in high density fed batch cultures

Ngày tải lên : 15/09/2015, 17:10

... limitation In addition, we found that maintaining a fixed glucoseto-glutamine ratio allowed for an indirect method of limiting glucose uptake Since glucose is being fed gradually by being linked to ... glutamine and glucose concentrations compared to batch so that feeding could be initiated earlier at f15 –18 h after seeding Once feeding had been initiated, glutamine concentrations can be maintained ... setpoint None Indirect glucose control through tagging of glucose to glutamine at a molar ratio of 5:1 0.5 0.5 4.0 4.0 Profile feeding to maintain glutamine at 0.3 mM 0.35 0.70 ture glutamine...

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Sensibility study of flooding and drying issues to the operating conditions in PEM fuel cells

Ngày tải lên : 05/09/2013, 14:58

... channel and the GDLs as in well as the channel inlet conditions ( SR in , RH in , Ptot , y in ) which give Qiin and yiin ISSN 2076-2895 (Print), ISSN 2076-2909 (Online) ©2010 International Energy ... focus on finding a pertinent location allowing to interpret the appearance of liquid water in terms of an ideal operating point 3.1.2 Onset of two-phase regime in the electrodes As mentioned in section ... change in temperature for the same operating current can lead to a drastic change in the cell humidification conditions, changing from flooding to drying or inversely Similar results are obtained in...

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Cell Cycle Regulation in Mammalian Germ Cells

Ngày tải lên : 25/10/2013, 21:20

... avail- Protein Name Cyclin dependant kinase Cell cycle regulating D-cyclin Cyclin E2 Proliferation of germ cells (POG) Cyclin D-dependant kinase inhibitors p27 Kip1 a Cdk inhibitor Cdk2 CyclinD2 Gcd/Pog ... partner, cyclin D, are responsible for the initial phosphorylation of the Rb protein by regulating cyclin E/Cdk2 and lifting the S-phase inhibition by preventing binding of its inhibitor, p27 ... RNA-recognizing motif involved in splicing, transport, translation and stability of mRNA Knockout results in decrease in the survival of germ cells at the genital ridge RNA-binding protein reported...

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Hướng dẫn nạp mực máy in cho tất cả các loại

Ngày tải lên : 26/10/2013, 15:11

... cách cài Win Windows có ưu điểm cho phép bạn cài đặt từ ổ cứng Khi bạn có cài Windows dạng chuẩn iso bạn giải nén vào thư mục Tiếp tục bạn chạy file setup.exe có thư mục để cài đặt Windows Việc ... thêm cho đề tài thêm phong phú cám ơn nhiều ^^ Mình viết tất lỗi máy in Cartridge mực muốn vài bạn hợp tác để chủ đề thật đầy đủ pm nha Cái đơn giản thôi, bạn cần USB, win (XP, Vista, win 7) ... không in hay không xuống mực ý mực in phun để lâu ngày bị vón cục không bạn phải xử lý trước bơm cách ngâm nước ấm cho mực bị vón cục tan Cách Bơm HP 4160 (Thông dụng) - Tháo bình mực khỏi máy in: ...

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Tài liệu Chọn máy in cho văn phòng nhỏ ppt

Ngày tải lên : 21/01/2014, 04:20

... hộp mực máy in đắt đỏ Trong đó, máy in laser đạt tiêu chuẩn vàng in text xác Còn máy in màu laser gợi ý kinh tế cho vẽ, lưu đồ logo Inkjet cho người in ít, laser để in nhiều Nếu bạn in với số ... chọn máy in đa Bạn tham khảo kinh nghiệm để chọn máy in cho phù hợp Máy in phun cho đồ họa, laser cho text Khi lựa chọn công nghệ máy in bạn cần xác định ứng dụng thường xuyên Bạn thường in in màu, ... trang ngày lòng với máy in màu inkjet Một số mẫu máy in phun inkjet định hướng cho văn phòng có tốc độ in nhanh hẳn, sức chứa khay giấy lớn chất lượng in text giấy thường Máy in đa - đầu tư hiệu...

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Tài liệu Báo cáo khoa học: Pyruvate reduces DNA damage during hypoxia and after reoxygenation in hepatocellular carcinoma cells pptx

Ngày tải lên : 18/02/2014, 16:20

... cells in a culture medium containing [13C3]pyruvate, under normoxia and hypoxia, for h Fig Effect of pyruvate on glutathione content during hypoxia ⁄ reoxygenation in HepG2 cells Cells were incubated ... cancer cells under such stressful conditions [36] Our present work provides new insights into the role of pyruvate in tumor cells during hypoxia We show here that tumor HepG2 cells are inclined ... shown) This is in line with our previous work [29] indicating enhancement of glycolysis activity in the presence of pyruvate Increased glycolysis might thus maintain the ATP supply during oxygen...

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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx

Ngày tải lên : 19/02/2014, 16:20

... and stained for F-actin with rhodamine-conjugated phalloidin The eﬀects of toxin B and latrunculin B on the actin ﬁlaments was examined by preincubating the cells with either 10 ngÆmL)1 toxin B ... levels in response to anisomycin, suggesting that an intact actin cytoskeleton is also necessary for anisomycin signaling Surprisingly, treatment of the cells with latrunculin B alone induced an increase ... further incubating the cells with actinomycin D (10 lgÆmL)1) for the indicated periods Likewise, the role of RhoA and p38 signaling was examined by preincubating the cells with the indicated drugs...

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Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf

Ngày tải lên : 19/02/2014, 16:20

... enrichment in iNOS activity within the particulate fraction of epithelial cells Apical distribution of iNOS in intestinal epithelial cells To get more insight into the localization of iNOS in intestinal ... localization of iNOS to apical domains of intestinal epithelial cells Particulate fraction association of iNOS in vivo In order to determine the distribution of iNOS in intestinal epithelial cells in vivo, ... the C-terminus of iNOS promotes in vitro interactions with the PDZ protein EBP50 [13] Interestingly, EBP50 has different binding partners including ezrin that can be anchored to the actin cytoskeleton...

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Tài liệu Báo cáo khoa học: Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells docx

Ngày tải lên : 19/02/2014, 16:20

... 4A), it was intense in VSMC in three distinct active RA sections (Fig 4Ba– c) In contrast, staining of synovial lining cells and sublining interstitum (Fig 4Ba–c), as well as cartilage chondrocytes ... showed that, in RA tissues, sPLA2-IIA is distributed in various cells, such as synovial lining and sublining cells and vascular cells, as well as in extracellular matrix ﬁbers [46] In our study, ... sPLA2IIE is induced rather speciﬁcally in VSMC in synovial tissues with active RA In inactive RA sections, sPLA2-IIF showed sporadic and weak staining in individual cells (Fig 4Ca,b), and a few interstitial...

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Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

Ngày tải lên : 19/02/2014, 17:20

... [s)1] 10)6 ã kass [M)1ặs)1] Cathepsin F Cathepsin K Cathepsin V Cathepsin S Cathepsin H Cathepsin C Cathepsin X Papain Cathepsin La Legumainb Cathepsin Ba Papaina 0.17 0.05 0.35 0.15 0.30 0.15 ... are listed in Table Cystatin F was observed to be a tight binding inhibitor of cathepsins F, K, L, V, with Ki values ranging from 0.17 to 0.35 nm Surprisingly cathepsin S, despite being an endopeptidase, ... Cystatin F in U937 cells lower inhibition constants for cathepsins S and H was due mainly to the low kass values Colocalization of cystatin F and potential target enzymes in U937 cells Using confocal...

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Tài liệu Báo cáo khoa học: Altered deoxyribonucleotide pools in T-lymphoblastoid cells expressing the multisubstrate nucleoside kinase of Drosophila melanogaster doc

Ngày tải lên : 20/02/2014, 01:20

... retained enzymatic activity in these human T-cell lines Increasing sensitivity to nucleoside analogs in Dm-dNK expressing cells We determined the sensitivity of the untransduced H9 and CEM cells ... expressing H9 cells, as found in the same cells grown in dialyzed medium without HU Effects on mtDNA In the light of the changed dNTP pools in Dm-dNK expressing H9 cells, we wanted to investigate ... Altered dNTP pools in T-cell lines expressing Dm-dNK DNA, RNA and protein synthesis in the Dm-dNK gene transduced cells The extent of DNA, RNA and protein synthesis in Dm-dNK expressing cells was compared...

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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

Ngày tải lên : 21/02/2014, 01:21

... suggested function of cystatin F is hypothetical Although most cystatins inhibiting cathepsin L also inhibit cathepsin S, an inhibitory activity of cystatin F against cathepsin S has not yet been reported ... cystatin F in U937 cells The high portion of intracellular cystatin F led us to fractionate cells to determine the localization of the inhibitor Initially cells were fractionated in a debris ... stored in granules or found in vesicles following uptake from the medium Ó FEBS 2002 Cystatin F in U937 cells (Eur J Biochem 269) 5507 A B Fig Immunocytochemical staining of cystatin F U937 cells...

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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx

Ngày tải lên : 21/02/2014, 03:20

... RNA extraction, cells were incubated for 24 h either in the absence or in presence of FSH, dbcAMP, cholera toxin or bFGF, either in combination with FSH or dbcAMP or cholera toxin and bFGF Extraction ... age The bFGF binding requires speci®c motifs on highly sulfated HS chains Sodium chlorate, in inducing structural alteration of HS chains, prevents the bFGF binding Our results indicate that ... sulfation on proteins and carbohydrate residues in intact cells without inhibiting cell growth or protein synthesis [35±37], and proteoglycan sulfation in cultured Sertoli cells from 20-day-old...

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Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx

Ngày tải lên : 22/02/2014, 07:20

... naive T cells [40] Dendritic cells are involved in the polarization of the immune response towards a Th1 (large production of interferon-c) or a Th2 (sustained production of interleukins-4 and ... in both human immature dendritic cells and in rat Ó FEBS 2002 The endocannabinoid system in human dendritic cells (Eur J Biochem 269) 3775 Fig FAAH, CB1 and CB2 mRNA expression in dendritic cells ... LPS, interleukin-12 and interferon-c, and upregulated by interleukins-4 and -10 [26,13] Taken together, these data show the presence of a complete endocannabinoid system in human dendritic cells...

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Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf

Ngày tải lên : 07/03/2014, 03:20

... of 575 nm (A) Fluorescence intensity analysis of intracellular adriamycin in gastric cancer cells (B) Adriamycin releasing index of gastric cancer cells The releasing index = (accumulation value ... characterized previously [26] The cells were maintained in RPMI-1640 containing 10% heat-inactivated newborn bovine serum (SiJiQing, Hangzhou China) at 37° C in a humidiﬁed atmosphere of 5% CO2 ... accumulation in SGC7901/PrPC cells were signiﬁcantly increased The results indicate that inhibition of the PI3K/Akt signaling pathway may lead to inhibition of the MDR induced by PrPC in gastric cancer cells...

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