plus strand rna and double strand rna viruses
Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... Joyce, G.F (1992) Randomization of genes by PCR mutagenesis PCR Methods Applications 2, 28–33 29 Shaklee, P.N (1990) Negative -strand RN A replication by Qb and MS2 positive strand replicases Virology ... assistance, and Cristiane Dinis Ano Bom and ´ Professors Fabio Almeida and Ana Paula Valente from CNRMN/ UFRJ for helpful comments and suggestions This work was supported, in part, by an International ... are shown as blue and green ribbons and the RNA molecule is shown in red (space fill) Met88 is represented in brown (at the dimer surface), Thr45 in yellow (interacting with RNA) and Asp11 in red,...
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Báo cáo khoa học: A new and efficient method for inhibition of RNA viruses by DNA interference pptx
... s-DNA (ssDNA) siRNA dsRNA siDNA Scrambled dsDNA 470 bp 22 nucleotides 20 nucleotides 21 bp 284 bp 21 bp 384 bp TMV TMV TMV TMV TMV TMV TMV RNA RNA RNA RNA RNA RNA RNA 203–674 of TMV RNA 483–504 390–409 ... hybridization of sense strand (5¢-GTTGCTCTGGAAAACTCATTT-3¢) and antisense strand (5¢-ATGAGTTTTCCAGAGCAACTT-3¢), and siRNA was synthesized using DNA templates (sense strand, 5¢-AAATGAGTTTTCCAGAGCAACCCTGTCTC-3¢; ... the replicase gene of TMV RNA (A) Half-leaves were inoculated with TMV and either 22 nucleotide sense strand DNA, 20 nucleotide antisense strand DNA, 470 bp dsDNA, or siRNA Tobacco leaves with...
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báo cáo khoa học: " DNA ligase 1 deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks" potx
... wild-type and atlig1-RNAiA lines B) WT and atlig1RNAi plants photographed weeks after germination Adaxial leaf from WT (C) and atlig1-RNAi lines (D) Abaxial surface of WT (E) and atlig1-RNAi lines ... of DNA double strand break repair in atlig1-RNAi lines Single cell electrophoresis under neutral conditions was used to analyse the repair of DNA double strand breaks in the wild type and silenced ... repair in Arabidopsis Col0 and atlig1-RNAiA and atlig1-RNAiB lines determined by neutral comet assay Figure DSB repair in Arabidopsis Col0 and atlig1-RNAiA and atlig1-RNAiB lines determined by...
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Studies on translational mechanisms of RNA viruses
... result in truncated and in most cases non-functional proteins (Donner and Kurland, 1972; Edelmann and Gallant, 1977a and b; Jelenc and Kurland, 1979; Jørgensen et al., 1993; Kurland and Gallant, 1996; ... showed internal initiation presence is on picornaviruses (Pelletier and Sonenberg, 1988b; Jang et al., 1988 and 1989) The well characterized 5’-UTR (untranslated region) of picornaviruses indicates ... viral RNAs have similar structures to the 5’-cap and 3’-poly (A) tail mimicking eukaryotic mRNA to compete for translation apparatus However, most RNA viruses have been shown to evolve alternative...
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Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf
... The double deletion strains were constructed as follows The haploid strains VZ1 (D7) and MES8 (D6), VZ2 (D7) and LLD9 (D8), JDP1 (D9) and LLD9 (D8), JDP2 (D9) and UBL2 (D10), were mated and the ... arrows labeled (b) and (c) point to the areas of interaction between the transmembrane helix of subunit and helices G and H1 of cytochrome b and between the N-terminus of subunit and helix a of cytochrome ... prototrophic for both markers Haploid spores of VZ4, VZ6, VZ14 and VZ9 were then selected for Trp+ and Leu+, Trp+ and His+, His+ and Ura+, or His+ and Leu+ prototrophy, respectively Other yeast genetic...
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book of exercises to accompany e. friedrich richter's manual of simple and double counterpoint (1888)
... varied manner; it can be utilized as cantus jirmus for the soprano, alto, tenor and bass, and the motion in halves and quarter-notes can also be applied in the different voices; hence a single ... farther, and wrote supplements to the separate chapters of the "Manual", so far as seemed necessary for better understanding rectly, them, moreover I developed more fully certain subjects only and ... supplements and extensions have been added to the book of exercises under the title: briefly indicated in them, PREFACE IV "Supplement to Eenst Friedk Richtee's Manual of simple and double Counterpoint"...
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Báo cáo hóa học: " Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses" pdf
... successful isolation of Dengue type 2, and viruses (Table 1) RNA was extracted from culture supernatant and used as template in RT-PCR and nested PCR The RT-PCR and nested PCR revealed desired amplification ... RT-PCR as described below RNA Extraction RNA was extracted from standard viruses, virus isolates, sera of suspected dengue patients and healthy volunteers using QIAquick viral RNA mini Kit, following ... over a period of six years from India Preparation of RNA standard The detection limit of this assay was determined using RNA standards The RNA standard was produced using T7 transcription kit (MBI...
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Báo cáo toán học: " Tight Quotients and Double Quotients in the Bruhat Order" doc
... member of the double coset W i wWJ , it follows that if x−1 and y −1 are in the same double cosets as wk and wl (respectively), then θ, xωi θ, yωi ⇔ k l, and equality occurs if and only if k ... for all i and j, ωi is dominant, and the W -stabilizer j of ωi is the parabolic subgroup W i the electronic journal of combinatorics 11(2) (2005), #R14 11 A Orbit stratification and the double weight ... (and thus (Ψ,
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Báo cáo y học: " Estimating the impact of expanded access to antiretroviral therapy on maternal, paternal and double orphans in sub-Saharan Africa, 2009-2020" ppt
... primary outcomes were the number of maternal, paternal and dual AIDS orphans in each country at year 2020 following varying scenarios of ART uptake Maternal and paternal AIDS orphans were defined as ... population size and HIV survival rates using published ranges for census [23,27-29] and empirical cohort [23,34,35] data Results Table presents the projected number of maternal, paternal, double and total ... ART access was expanded gradually This included an estimated 3.15 million maternal orphans, 1.89 million paternal orphans and 748,320 double orphans Anema et al AIDS Research and Therapy 2011,...
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Báo cáo y học: " Improved hatchability and efficient protection after in ovo vaccination with live-attenuated H7N2 and H9N2 avian influenza viruses" ppsx
... we sought to examine whether the mH7 and mH9 att viruses viruses showed similar replication yields as unmodified H7 and H9 att viruses, and if these modified viruses were more amenable for in ovo ... cleavage site in the H7 and H9 subtypes would result in more attenuated vaccine viruses and improved hatchability, we generated the recombinant viruses 2mH7N2:6WF10att and 2mH9N2:6WF10att Modifications ... infection and then fixed and the viral antigen was visualized by immunostaining as described in Materials and Methods The plaques sizes were observed and the plaque numbers were counted and calculated...
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Báo cáo y học: " A rare association of deformities with diplopodia, aplasia of the tibia and double fibula: A case report" pps
... 1999, 60(5):272-82 Narang IC, Mysorekar VR, Mathur BP: Diplopodia with double fibula and agenesis of tibia – a case report Journal Bone Joint Surg (Br) 1982, 64(2):206-209 Igou RA Jr, Kruger LM: ... Authors' contributions SAK identified the case and prepared the manuscript AK and MKV helped in manuscript preparation All the authors have read and approved the final version of the case report ... publish this case report and any accompanying images A copy of the written consent is available for review by the Editor-in-Chief of this journal Publish with Bio Med Central and every scientist can...
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Báo cáo y học: " A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses?" docx
... gene for WEEV and NSP3 gene for EEEV, and the sequence analysis confirmed the specificity for WEEV and EEEV, respectively Using other encephalitis viruses RNA (e.g., DEN, CHIK, TBE and JEV) as ... cowrote and edited the manuscript YL, H L, FL, XC, TS and GC took samples and did laboratory testing QZ and YY organized the overall project and helped edit the manuscript All the authors read and ... tissues were grinded, and the RNA was extracted and applied with the duplex real-time RT-PCR assay Results Sensitivity and specificity analysis of the duplex system Figure and Table show the sensitivity...
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Báo cáo khoa học: "Development of real time PCR for detection and quantitation of Dengue Viruses" potx
... evaluated using RNA extracted from virus stocks of two related Flaviviruses (JE and WN), and CHK virus, which causes a dengue like disease and is transmitted by the same vector In addition RNA extracted ... DENV-1 to viruses, (B) standard curve of the in vitro transcribed RNA and (C-F) standard curves for the four DENV serotypes Page of (page number not for citation purposes) Virology Journal 2009, ... 5'end and a minor groove binder (MGB) and a non fluorescent quencher at the 3'end The specificity of primers and probe were tested against the four serotypes of DENV, JE, WN, and CHIK viruses...
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Báo cáo khoa học: " Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities" doc
... reduces the spread of influenza viruses in epidemic and pandemic areas Methods Viruses and cells Viruses were propagated in 10-day-old embryonated chicken eggs at 34°C, and after 48 h of incubation, ... influenza A viruses to sialoglycopolymers and guinea pig erythrocytes and inhibition by PVP-I In agreement with hemagglutinins from avian and human influenza viruses, which prefer binding to α2,3- and ... Virology Journal 2009, 6:124 Background Among the three types (A, B and C) of influenza viruses, A type is the most virulent, infecting various avian and mammalian species and causing human pandemics...
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Báo cáo y học: " Use of different but overlapping determinants in a retrovirus receptor accounts for non-reciprocal interference between xenotropic and polytropic murine leukemia viruses" ppt
... NRI also occurs among avian sarcoma and leukosis viruses (ASLV) types B, D, and E Viruses of types B and D can interfere with each other as well as type E viruses, whereas ASLV-E can interfere ... with HpaI and BamHI Additional retroviral vectors used here included LAPSN [6], which encodes AP and Neo, and LN [40], which encodes Neo Viruses and infection assays The AKR6 xenotropic and 1E polytropic ... Fluorescence Figure 1E SU Binding and interference properties of cloned AKR6 SU and Binding and interference properties of cloned AKR6 SU and 1E SU (A) LAPSN(AKR6env) and LAPSN(10A1env) vector titers...
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A PROSPECTIVE STUDY ON DETECTION, SUBTYPE ANALYSIS, CHARACTERIZATION, MOLECULAR EPIDEMIOLOGY AND TRANSMISSION OF INFLUENZA VIRUSES AMONG UNIVERSITY STUDENTS AND STAFF IN SINGAPORE
... H3N2 viruses 6.3.1.1 HA and NA diversity 6.3.1.2 Diversity of internal genes 6.3.2 Seasonal H1N1 viruses 6.3.2.1 HA and NA diversity 6.3.2.2 Diversity of internal genes 6.3.3 Pandemic H1N1/09 viruses ... 95 ligand domain in NS1 (Obenauer et al 2006) This is present in H5N1 and pandemic 1918 H1N1 viruses (Jackson et al 2008) Notably, pH1N1/09 has truncated protein and hence lacks PDZ ligand domain ... et al 2010 Virological Military recruits S P & S- pandemic and seasonal flu; P stands for pandemic flu; S stands for seasonal flu; pH1N1- pandemic 2009 H1N1 NUH- National University Hospital;...
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Eating out en francais more than 2,000 food and wine terms in english and french plus mini phrasebook and guide to wine regions (english and french edition) a c
... [bird] aux amandes with almonds põte damandes almond paste amandine almond tart amer (amốre) bitter amộricaine, sauce the cooking liquor from lobster mixed with the lobster coral and cream amoricaine, ... mushroom champignons de Paris button mushrooms champignons farcis stuffed mushrooms chandelier candlestick chandelle candle chanterelle chanterelle [mushroom] Chantilly (with) whipped cream chapelure ... croque-madame fried cheese and ham sandwich topped with a fried egg croquembouche pyramid of profiteroles with caramel, served at weddings croque-monsieur fried cheese and ham sandwich 32 curry croquette...
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5515 comparison repeated comparative and double comparatives
... interesting worse and worse later and later warmer and warmer messier and messier more and more expensive more and more violent more and more difficult 10 better and better DOUBLE COMPARATIVES (IDEAS) ... and …… his old one Obviously, it is .…………… It is ……… model in the market (large/fast/expensive/new) PAGE REPEATED COMPARATIVES more and more boring more and more interesting worse and ... better and better when the weather is warm The warmer the weather, the better I feel I like it more when things are dangerous As Mark is getting old, he looks more and more like his grandfather...
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BÀI GIẢNG kế TOÁN QUỐC tế chapter 5 ledger accounts and double entry
... created or an asset realised 10 The journal and imprest system Journal • Format of journal entries is as follows Narrative to explain transaction Remember: the journal is used to keep a record of unusual ... assets and liabilities is preserved • In a system of double entry bookkeeping every accounting event must be entered in ledger accounts both as a debit and as an equal but opposite credit Double ... Objectives The nominal ledger The accounting equation Double entry bookkeeping The journal and imprest system Day book analysis The receivables and payables ledgers The nominal ledger Ledger accounting:...
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