plc pkc cascade and ca2 signaling
Tài liệu PLC Programming Methods and Applications P2 pptx
... fundamental understanding of the various types of PLCs available, the advantages and disadvantages of each, and the way in which a PLC executes a program The open frame, shoebox, and modular PLCs are each ... a typical PLC ” how programs are stored in a PLC ” the equipment used to program a PLC ” the way that a PLC inputs data, outputs data, and executes its program ” the purpose of the PLC update ... withstand vibration, shock, elevated temperatures, and electrical noise to which manufacturing equipment is exposed As more manufacturers become involved in PLC production and development, and PLC...
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Tài liệu PLC Programming Methods and Applications P1 docx
... Fundamentals SWITCH1 and SWITCH2 and SWITCH3 SWITCH1 and SWITCH2 and SWITCH4 SWITCH1 and SWITCH3 and SWITCH4 SWITCH2 and SWITCH3 and SWITCH4 SWITCH1 and SWITCH2 and SWITCH3 and SWITCH4 SWITCH1 ... Diagram Fundamentals recommended In a PLC, the ordering of the rungs is critical and rearranging the order could change the way the PLC program executes AND OR and OR AND Let us now complicate the circuitry ... energize a rung For this OR -AND circuit, LAMP4 will be lit when the following combinations of switches are on: SWITCH1 and SWITCH3 SWITCH1 and SWITCH4 SWITCH2 and SWITCH3 SWITCH2 and SWITCH4 1-18 Chapter...
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Plc programming methods and applications
... Fundamentals SWITCH1 and SWITCH2 and SWITCH3 SWITCH1 and SWITCH2 and SWITCH4 SWITCH1 and SWITCH3 and SWITCH4 SWITCH2 and SWITCH3 and SWITCH4 SWITCH1 and SWITCH2 and SWITCH3 and SWITCH4 SWITCH1 ... fundamental understanding of the various types of PLCs available, the advantages and disadvantages of each, and the way in which a PLC executes a program The open frame, shoebox, and modular PLCs are each ... first PLCs were developed and why they were better than the existing control methods ” the difference between the open frame, shoebox, and modular PLC configurations, and the advantages and disadvantages...
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Tài liệu Báo cáo khoa học: Regulatory modes of rod outer segment membrane guanylate cyclase differ in catalytic efficiency and Ca2+-sensitivity ppt
... between GCAP-1 and GCAP-2 in their regulatory properties and if so, what is the physiological significance of such a difference? The study demonstrates that GCAP1 and GCAP2 modulate the Ca2+ signaling ... °C with a Ca2+ -sensitive electrode (World Precision Instruments, Inc.) using calibration standards in the range from 10)8 to 10)1 M free [Ca2+ ] Results The Ca2+ -sensor proteins GCAP-1 and GCAP-2 ... HEK293 cells and COS cells Cell membranes were reconstituted with D6SGCAP-1 and GCAP-2 and the EC50 and IC50 values were determined EC50 values were similar as described previously [25,44] and maximal...
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Báo cáo khoa học: Regulation of Cyr61/CCN1 gene expression through RhoA GTPase and p38MAPK signaling pathways Role of CREB and AP-1 transcription factors doc
... isolated and hybridized to Cyr61/CCN1, CTGF/ CCN2 and GAPDH cDNA probes and a plasmid vector, which had been slot-blotted on nylon membranes The hybridization signals were measured and standardized ... and p38 signaling in the activation of CREB via MSK1 Role of RhoA and p38 kinase in the activation of Cyr61/CCN1 promoter Fig Immunoblot analyses of RhoA activation and p38 MAP kinase, CREB and ... A-fos and A-ATF-2 proteins Immunodetection of endogenous c-fos and ATF-2 was performed with anti-c-fos and anti-ATF-2 Igs, respectively, using the same cell lysates Detection of K-CREB and endogenous...
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Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx
... adipose and may play a role in breast tumor development [13,14] Nuclear hormone receptors are typically ligand-activated transcription factors and their activities are modulated by coactivators and ... 289R and K289 ⁄ 329R not shown) However, triple-mutations with three arginine substitutions at 173, 213 and 289 or 289, 329 and 389 resulted in some LRH-1 localization in the nucleoplasm and a ... expression levels of E1, E2 UBC9 and SUMO-1 in HESCs However, our data show that the expression of both E1 and E2 is regulated by cAMP signaling in granulosa cells and Shao et al [46] demonstrated...
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Báo cáo Y học: A new siglec family member, siglec-10, is expressed in cells of the immune system and has signaling properties similar to CD33 docx
... vector, Gibco/BRL, Grand Island, NY, USA) for several splice variants were obtained from Incyte and sequenced Sequencing and alignment Individual clone colonies were cultured and DNA was isolated ... II and Nco I The amplified fragment was cloned into pd19 by digesting the fragment with HindIII and Bgl II and the plasmid with HindIII and Bam HI The fragment was ligated into the plasmid and ... by standard transformation methods B-cell lymphoblastomas Ramos, Raji, Daudi and HSB-2, a T-cell lymphoblastoma Jurkat, a erythroblastic leukemia cell line HEL and monocytic cell lines U973 and...
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Báo cáo khoa học: Voltage- and Ca2+-activated potassium channels in Ca2+ store control Ca2+ release docx
... force for Ca2+ efflux When Ca2+ is released, the Ca2+ efflux may be amplified by Ca2+ induced Ca2+ release (CICR) at the Ca2+ releasing site (Fig 4E, left) A decrease in luminal [Ca2+ ] and a negative ... from the store and hence develops later than the Ca2+ efflux The Ca2+ flux can be estimated by the derivative of fluo-4 trace (D [Ca2+ ]i), and the efflux of Ca2+ precedes the main part of Ca2+ rise (Fig ... noted here that Ca2+ fluxes (rates of charge movements by Ca2+ , i.e Ca2+ currents) generate the voltage drops across the membrane of Ca2+ store A rise in [Ca2+ ]i is the integration of Ca2+ effluxes...
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Báo cáo khoa học: Binding of the viral immunogenic octapeptide VSV8 to native glucose-regulated protein Grp94 (gp96) and its inhibition by the physiological ligands ATP and Ca2+ Ming Ying and Torgeir Flatmark pot
... ðMr;analyte =Mr;ligand Þnð0:4ÞRimmobilized ððdn=dcanalyte Þ=ðdn=dcligand ÞÞ ð2Þ where (dn ⁄ dcanalyte) and (dn ⁄ dcligand) are the refractive index increment for the analyte and ligand, respectively ... 2006 FEBS 515 Grp94–ligand interactions M Ying and T Flatmark and a negative DRU due to the ligand-induced conformational change, no exact value for the binding affinity and the stoichiometry of ... 230 RU, and the binding was studied at 25 °C and pH 7.4 in HBS-P buffer (A) Representative sensorgrams obtained at 100, 400, 1000 and 3000 lM Ca2+ (B) The equilibriumbinding isotherms for Ca2+ (d),...
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Báo cáo khoa học: The two IQ-motifs and Ca2+/calmodulin regulate the rat myosin 1d ATPase activity pptx
... 100 lM free Ca2+ (Ca2+ , lanes and 3) or in the absence of free Ca2+ (EGTA, lanes and 5) To determine the amount of calmodulin bound to Myo1d-IQ2, F-actin was added to the samples and they were ... absence and presence of free Ca2+ Puried Myo1dDLV-IQ2 (0.5 lM) was incubated with 10 lM CaM either in the absence of free Ca2+ (EGTA, lanes and 4) or in the presence of 100 lM free Ca2+ (Ca2+ , ... the free Ca2+ -concentration Myo1d-IQ1 puried in the presence of EGTA was either left in EGTA (EGTA, lanes and 4) or buffer conditions were adjusted to 100 lM free Ca2+ (Ca2+ , lanes and 2) followed...
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báo cáo hóa học:" Bioactivity-guided identification and cell signaling technology to delineate the immunomodulatory effects of Panax ginseng on human promonocytic U937 cells" potx
... biomolecular assays and data interpretation JL, JR and GC participated in study design and interpretation of results AL designed the study and led the data interpretation and manuscript writing ... Chemokine (C-C motif) ligand Chemokine (C-X-C motif) ligand 11 Chemokine (C-C motif) ligand Interleukin 18 receptor Interleukin 1, beta Interleukin 1, beta concentration and the percentage of each ... identified and quantified by retention time and peak areas, respectively, as compared to the commercially available pure standards Nine ginsenosides including Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg3 and...
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báo cáo hóa học: " Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury" potx
... were then stripped and reprobed with p44/42 MAPK (ERK1/2) mouse monoclonal Ab (1:1000; Cell Signaling) to confirm equal protein loading The films were subsequently scanned, and band intensities ... field (CA2) and blood vessel (BV) Magnifications are × 400 *P < 0.05, **P < 0.01, and ***P < 0.001 vs sham-injured mice; #p < 0.05, ##p < 0.01, ###p < 0.001 vs placebo mice; + +p < 0.01, and +++p ... (5-6 mice per group) were carefully dissected and collected, snap frozen in liquid nitrogen, and stored at -80°C Different regions (cortex, hippocampus and striatum) were used for total RNA extraction...
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Báo cáo hóa học: " Resveratrol engages AMPK to attenuate ERK and mTOR signaling in sensory neurons and inhibits incision-induced acute and chronic pain" pptx
... (Figure 3A) and a reversible inhibition of both ERK (Figure 3B and C) and mTOR signaling (Figure 3D–H) Hence, resveratrol dose- and time-dependently activates AMPK and inhibits ERK and mTOR signaling ... resveratrol profoundly inhibits ERK and mTOR signaling in sensory neurons in a time- and concentration-dependent fashion and that these effects are mediated by AMPK activation and independent of sirtuin ... pain and prevention of chronic pain induced by incision They show that resveratrol potently and efficaciously inhibits ERK and mTOR signaling in sensory neurons in vitro The mTOR [39-42] and ERK...
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Molecular and Cellular Signaling docx
... organs, and glands that continually send and receive chemical messages Cells of the immune systems send and receive cytokines; cells residing in glands of the endocrine system secrete hormones; and ... diseases, drugs, and signaling are made The target audience for this text includes students in chemistry, physics, and computer science who intend to work in biological and medical physics, and bioinformatics ... Docking Sites and Substrate Specificity 6.11 Protein Phosphatases Are Prominent Components of Signaling Pathways 6.12 Scaffold and Anchor Protein Role in Signaling and Specificity...
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Báo cáo sinh học: "Dishevelled and Wnt signaling: is the nucleus the final frontier" potx
... phospholipase C (PLC; not shown), calcium-calmodulin kinase (CamK2) and protein kinase C (PKC) This pathway also uses the PDZ and DEP domains of Dsh to modulate cell adhesion and motility Note ... protein kinase C (PKC) [9,18] The Wnt -Ca2+ pathway is important for cell adhesion and cell movements during gastrulation Signal specificity With such a daunting number of Wnt ligands and Frizzled ... whether (and if so which) Wnt ligands are specific to particular pathways, and how signals are channeled to each pathway Notably, some Wnt ligands are known to activate both canonical and non-canonical...
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Báo cáo y học: " Eph Receptors and Ephrin Signaling" pps
... receptors and integrin signaling [7] Reverse Signaling The interaction between ephrin ligands and Eph receptors results not only in forward signaling through the Eph receptor, but also in ‘reverse’ signaling ... anchor EphrinB ligands are transmembrane proteins with a cytoplasmic tail and PDZ binding domain Bi-directional signaling results in forward signaling through Eph receptors and reverse signaling through ... ephrin ligands Bi-directional Signaling An important property of interactions between Eph receptors and ephrin ligands is the bi-directional signaling that results due to activation of signaling...
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Báo cáo y học: "Activation of WNT and BMP signaling in adult human articular cartilage following mechanical injury" potx
... encoding WNT ligands (WNT-1, WNT5a, WNT-5b, WNT-9a/14, and WNT16), receptors (FRZD-1 and FRZD-7), intracellular mediators such as β-catenin, and downstream transcription factors such as TCF and LEF-1 ... patellar dislocation was then reduced and the joint capsule and the skin sutured in separate layers The mice were then allowed to walk freely in standard cages and maintained on free diet Control ... then reduced and the joint capsule and the skin sutured in separate layers and the mice allowed to walk freely The animals were killed at different time-points for histological and histochemical...
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Calcium-sensing receptors regulate cardiomyocyte Ca2+ signaling via the sarcoplasmic reticulum-mitochondrion interface during hypoxia/reoxygenation pdf
... the indicator in the absence of Ca2 +and is obtained by adding a solution of 10 mM EGTA for 15 min, and Fmax is the fluorescence of the Ca2+ -saturated indicator and is obtained by adding a solution ... cardiomyocyte Ca2+ signaling through MAM subjected to CaR activation and H/Re The main findings of this study are as follows: (i) Activation of CaR induced the release of Ca2+ from the SR and, simultaneously, ... class of G-proteins and stimulate Phospholipase C, resulting in phosphoinositide(PI) hydrolysis and the formation of inositol triphosphate and diacylglycerol IP3Rs are ligand-gated Ca2+ channels that...
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báo cáo khoa học: "Different patterns of NF-B and Notch1 signaling contribute to tumor-induced lymphangiogenesis of esophageal squamous cell carcinoma" ppt
... characteristics and their associations with NF-B and Notch1 signaling were also measured to determine Page of the contribution of NF-B and Notch signaling to tumor-induced lymphangiogenesis Materials and ... ZC and HL conceived of the study; CS, YS, YL, YL and BZ performed experiments; ZC and LC analyzed data and prepared the figures; CS, ZC and HL drafted the manuscript All authors have read and ... an inverse relationship between Notch1 and NF-B signaling and tumor-induced lymphangiogenesis Taken together, our findings imply that Notch1 and NF-B signaling have counter-acting roles in...
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báo cáo khoa học: " Transcriptional responses to polycyclic aromatic hydrocarbon-induced stress in Arabidopsis thaliana reveal the involvement of hormone and defense signaling pathways" pdf
... phenanthrene and ethylene signaling Without PAH, the ethylene overproducer eto3 and the constitutively -signaling triple mutant grew short hypocotyls and roots, consistent with the standard model ... sensitivity, and the etr1-6;etr2-3;ein44 [34] triple ethylene receptor mutant which exhibits constitutive ethylene signaling Because the WT and ethylene signaling mutants differ in root and shoot ... phenanthrene) and control plants (0 mM phenanthrene) were grown under long days and harvested at 21 d, and at least 20 plants were pooled and stored at -80°C 500 mg tissue was removed from each pool and...
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