... cho hoạtđộng chức quan phận Thứ tự ưu tiên tích luỹ sử dụng chất dinh dưỡng sau : (1) Hoạtđộng thần kinh (4) Tích luỹ nạc (2) Hoạtđộng sinh sản (5) Tích luỹ mỡ (3) Phát triển xương Bộ xương Mô ... Excel xử lý thống kê theo phương pháp phântích phương sai (ANOVA) quamôhình tương quan tuyếntính GLM (General Linear Model ) phần mềm Minitab version … 42 KẾT QUẢ VÀ THẢO LUẬN Sau tháng theo dõi ... 22 axit amin quan trọng dinh dưỡng động vật (Vũ Duy Giảng, 1999, [6]) Dựa vào cấu trúc lạp thể người ta phân thành dạng L D axit amin, tất axit amin (trừ Glycine) mang hoạttính quang học, dạng...
... monitored using a Lambda ¨ spectrophotometer (PerkinElmer Instruments, Uberlingen, Germany) In order to quantify the precorrin-2, it was converted to sirohydrochlorin (e376 = 2.4 · 105 m)1Æcm)1 [35])...
... [14] The enhanced cofactor binding is thought to result from MSR-catalysed reduction of exogenous aquacob(III)alamin (AqCbl) to form cob(II)alamin Reduction of the Co centre promotes the dissociation ... recombinant hMS is expressed as an apoenzyme in P pastoris at levels that enable purification of sufficient quantities for functional analysis (Table 1) A clear advantage of using Pichia as a heterologous ... varying concentrations of MSR and 100 lM NADPH The experimental data were fitted to a hyperbolic equation, and yielded a Kact value for MSR of 107 ± nM and a Vmax value of 2.0 ± 0.1 nmolÆmin)1 (B)...
... Biochem Pharmacol 63, 381–391 44 Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, ... methion43.19; H, 5.00; N, 18.31 C8H9N3Æ2HClÆO.2H2O requires: ine was eluted with mL of water and quantified using C, 42.95; H, 5.14; N, 18.78% a liquid scintillation counter (Packard Tricarb 1900CA; ... optimization Ligands were parameterized using partial atomic charges and other values obtained from quantum mechanic modelling (HartreeFock 6–31G*) of the ligand structure using pc spartan pro (Wavefunction...
... DNase I and reverse-transcribed into cDNA Quantitative analyses of CXCL12 and CXCR4 transcripts were performed by real-time PCR SYBR Green I system The quantity of transcripts was normalized with ... depleted DNMT1 Quantitative western blot showed 90% reduction of DNMT1 protein in Jurkat T cell line Total RNA was isolated, treated with DNase I and reverse-transcribed into cDNA Quantitative ... potential was observed by fluorescence microscopy and quantitative analysis Release of cytochrome C by mitochondria was determined using a semi-quantitative sandwich ELISA kit These results show,...
... and JThr in Eqn (10) can be replaced by CGS and TS MichaelisMenten equations (Eqns and 7) yielding the following quadratic equation (Eqn 11) app ẵPhser2 JPhser kapp catCGS kcatTS ị ỵ Kapp JPhser ... between the computer model and the experimental data originated from an inadequacy of the TS mechanistic equation This equation correctly describes the interaction between TS and AdoMet in the presence ... The experimental points were tted to Hill equations The thick curves are ux values calculated with the computer model using CGS and TS mechanistic equations Input variables were set at the value...
... constants (Kd) of the proteinligand (LP) complexes were evaluated by the curve tting to the quadratic equation deduced from the equilibrium L + P , LP, considering that LP is proportional to the ... made for dilution effects (never exceeding 10%) The quenching data were tted to the SternVolmer equation, =F F ẳ ỵ KSV ẵQ 1ị where F and F are the uorescence intensities in the absence and presence ... ligands were also measured by the shortened of the emission lifetimes, according to the SternVolmer equation: s =si ẳ ỵ kq ịi s ẵQ i i 3ị where si and si are for the emission lifetime of the component...
... shows an almost equally elevated and high expression of AGAT mRNA in all three tumor samples, whereas it is almost undetectable in normal muscle GAMT mRNA expression was almost equal in all three ... that the activities of these two enzymes in the three tumors (EAC, S180 and sarcoma tissue) were quantitatively more or less similar Arginase II activity was also quite high in these three tumors ... (A) AGAT and (B) GAMT: (a) immunoblot; (b) parallel gels stained with Coomassie blue to confirm equal protein loading; and (c) densitometric analysis of the amplified PCR fragments (mean ± SD; n...
... but not with Met, or pretreated with PG, EhMGL1 was no longer recognized by the antibody (the equal loading of proteins was verified by silver staining; Fig 2A), suggesting that EhMGL1 was chemically...
... R-factor (%) 15.8 (14.4) Rfree-factor (%) 19.1 (20.7) Quality of model ˚ r.m.s.d bond lengths (A) 0.013 r.m.s.d bond angles (°) 1.16 Ramachandran plot quality % in most favourable 90.9 region % in additional ... baseline) in Eqn (1) were set to zero The fits (nonlinear least-squares fitting) were generated using the algorithm of Levenberg and Marquardt and performed in the program 2452 Origin, version 6.0 ... ferricyt c-550 were carried out using the sitting drop vapour diffusion method at 295 K using equal volumes of protein and reservoir solutions Dark red crystals of the M100K variant suitable...
... (Brown et al., 1993), the statistical word alignment model can be generally represented as in equation (1) Pr(a, f | e) = Where e and f represent the source sentence and the target sentence, ... respectively In this paper, we use a simplified IBM model (Al-Onaizan et al., 1999), which is shown in equation (2) This simplified version does not take into account word classes as described in Brown ... model and unsupervised model, respectively λ is an interpolation weight We train the weight in equation (8) in the same way as described in Wu et al (2005) Pseudo Reference Set for Unlabeled Data...
... product/item/crop diversion problem/drug/disorder wildlife gle word does not describe the cluster adequately, or cases where there are a few good hypernyms which tend to alternate, such as "country"...
... Biochem J 301, 557–561 23 Bradford, M.M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, ... bacteria was performed in E coli XL-1Blue strain All other chemical and reagents were of the highest quality available Amplification of MAT2-encoding fragment To generate a DNA probe, the polymerase ... cells and libraries DNA modification and restriction enzymes were from Boehringer Mannheim Thermus aquaticus (Taq) polymerase was from Promega and Pyrococcus furiosus (Pfu) polymerase was from Stratagene...
... sample (20 lL) was eluted in the presence of M NaCl Polymalate in the eluate was hydrolysed and quantitated by the fluorimetric malate dehydrogenase assay An average amount of 0.069 ± 0.003 lg...
... The following supplementary material is available: Fig S1 Area quantitation of peaks obtained in polysomal profile analysis Fig S2 Quantitation of northern hybridizations Fig S3 Utp25p sequences ... from the same fractions were subjected to western blot for detection of ProtA and ProtA–Utp25p (C) Quantitation of the bands obtained from RNA co-ip by phosphorimaging Ratio of bound ⁄ input is shown ... 5¢-TGTTATCCTCTGGGCCCCG-3¢ 5¢-ATGGGGCTCATCAACCAAGTTGG-3¢ 44 47 44 44 48 44 49 44 50 Paulo State University-UNESP, Araraquara, SP, Brazil) [41]; rabbit anti-GFP, : 10 000 (kind gift from F Gueiros-Filho, University of...
... stereochemical quality of protein structures J Appl Crystallogr 26, 283–291 34 Schuck P (2000) Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and Lamm equation ... versus the concentration of the activation domain and the curves show the best fit of the data to the quadratic Eqn (1) Table lists the calculated dissociation constants for the wild-type and D963E ... components of the MS holoenzyme is required to ascertain if full-length hMS possesses higher order quaternary structure Fluorescence titration assays have demonstrated an interaction between the...
... labels; in the case of a tie (one vote each way) we assigned the relation as true or false with equal probability The evaluation of the syntactic, lexical, and combination of features at a recall...
... âm 20 oC để phântích thành phần hóa học thể cá theo phương pháp AOAC (2000) Các giá trị trung bình sinh trưởng hiệu sử dụng thức ăn độ lệch chuẩn tính chương trình Excell, phântíchthống kê ... protein Kết phântích (broken-line) mối tương quan tốc độ tăng trưởng đặc biệt hàm lượng methionine thức ăn y = 0,1439x + 1,4297 y= 0,0547x + 1,4297, y = 2,89 (hình 2) thể tương quan chặt chẽ ... biệt (SGR) cá tra giống Hình Sự tương quan hàm lượng methionine (g/kg protein) tốc độ tăng trưởng đặc biệt (SGR) cá tra giống Hiệu sử dụng thức ăn Hệ số thức ăn (FCR) hiệu sử dụng protein (PER)...