... release of ADPdepolymerised actindenaturedactinrandom aggregationafter release of pressure[1][2][3]Fig. 12. Schematic interpretation of thebehaviour of F-actin in the presence of free ... 250 MPa.However, the increase of intensity of ¯uorescence of eATPbuffer itself was much smaller than that of eATP bo und toG-actin. Therefore, the increase of ¯uorescence seems to b edue ... thecentre of spectral mass of intrinsic ¯uorescence spectrum of G-actinwithATP(0.5mgámL)1, p H 7.5) in a pressurerange from 0.1 MPa to 400 MPa at a đxed temperature of 20 C. The transition of the...
... findings alongside studies of hypoxic human skeletal muscle, putting them into the context of whole-body physiology and acclimatization to high-altitude hypoxia. A number of current controversies ... abundant in skeletal muscle and stimulate mitochondrial biogenesis via the activation of a number of transcriptional pathways [20,21]. Overexpression of PGC-1α in mouse skeletalmuscle leads to ... [39].Measurement of metabolic enzyme activities in the skeletal muscle of rats housed in hypoxic-hypobaric chambers has suggested that shifts towards glycolysis are dependent on muscle type as...
... Regulation of the rat sarcoplasmic reti-culum calcium release by calcium. J Muscle Res CellMotil 21, 131–138.20 Lai FA & Meissner G (1990) Structure of the calciumrelease channel ofskeletalmuscle ... presence of 18 nm ryanodineand the amount of ryanodine bound was plottedagainst the concentration of the gadolinium (Fig. 7).Fitting of data points with the Hill equation revealed aKdvalue of ... CoronadoR (1988) Ryanodine receptor ofskeletalmuscle is a gapjunction-type channel. Science 242, 99–102.13 Sitsapesan R & Williams AJ (1995) The gating of thesheep skeletal sarcoplasmic reticulum...
... standardization of the loading. (D) Weights of injected TA muscles (n = 13) were compared to those of control samples. No significant difference wasobserved. (E) Histological analyses of muscles. Frozen ... obtain denervation of the muscleof the lower limb,the dorsal skin of the thigh of 2-month-old male129SvPasIco mice (n = 6 for each time point) was cut,and the posterior muscles were split ... waschecked at the time ofmuscle excision by visualization of abnormal gait of the limb and by verifying the disconti-nuity of the sciatic nerve at the thigh. The TA, EDL andsoleus muscles were sampled...
... structure of TnI and TnC that can explainhow they interact during muscle contraction. Figure 6shows a schematic representation of the structural dis-position of TnC and TnI in the light of recent ... troponin complexand regulation ofmuscle contraction. FASEB J 9, 755–767.2 Gordon AM, Homsher E & Regnier M (2000) Regula-tion ofcontraction in striated muscle. Physiol Rev 80,853–924.3 ... domain of TnC [33]. The crystal structure of cTn [20] showed that residues R117 to L128 of TnI (inchicken fast skeletal numbers) are in close contact withthe N domain of TnC in the presence of...
... the mass of the testing end of myometer; amaxis the maximal amplitude of oscillation, and ∆l is thedepth of the displacement of the testing end.Measurements for determination ofmuscle tone ... characteri-zation of the skeletalmuscle may provide new insightsinto muscle function and can help to diagnose the stage of pathological processes of muscles.The testing end (mass 20 grams) of the computerizedmyotonometry ... during research (see subdivision of workers into 3 categories according to MYO data) areindicative of several features of HFF and LFF.Features of HFF:- Force of muscles is restored quickly after...
... markers ofskeletal muscle proteolysis (tyrosine and 3-methylhistidine) simultaneously increased in the skeletalmuscleof septic rats. Septic ratswere infused with insulin at a constant rate of ... conditions. Skeletalmuscle is the main target organ for hypercatabolism,and this phenomenon is a vital factor in the deterioration of recovery in septic patients. In skeletal muscle, activation of the ... longus muscle The proteolytic rate of skele tal muscle wa s measured asnet release of tyrosine for total protein and 3-MH formyofibrillar protein. Com pared to the control group , therate of total...
... viscosity of t he extract as a result of D NA, 2 lLofDNase I (200 units) was added per 100 lL of buffer [30].Following filtration through two layers of miracloth a nd theaddition of four volumes of ... usedwere of analytical grade and purchased from SigmaChemical Company.Preparation of total muscle extractsFor the comparative gel electrophoretic analysis of normalvs. dystrophic skeletalmuscle ... analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4.As the full-length Dp427 isoform of dystrophin does notenter the second dimension of conventional 2D gels, theexpression level of a-dystroglycan...
... partner ofskeletalmuscle titin-N2A [18]. An analysis of CARP expression in the subcellular compartmentsobtained from the muscleof the mice injected withpYFP-CARP-CFP-HIS confirms the presence of ... althoughthe over-expression of CARP in muscle cells results inNF-jB p65 inhibition, it does not affect the expression of MURF1, which is one of the main mediators of NF-jB-dependent muscle atrophy [51]. ... thecontrol of the NF-jB pathway because calpain 3deficiency is associated with an altered distribution of both NF-jB and of its regulator IjBa [8], as well aswith blockade of the induction of specific...
... 4. Immunoblot of CK isoforms of different post-operativehuman tissues. (A) Immunoblot of MCK (a) and sMitCK (b) of nor-mal muscle (N) and fibrosarcoma (T) tissue. (B) Immunoblot of BCK(a) and ... Expression of mRNA of MCK and sMitCK isoforms of crea-tine kinase and b-actin in normal muscle (NM) and 3MC inducedsarcoma tissue (ST) of mouse.1.00.80.60.40.2Relative expression of mRNA0.0NMCK ... that because of the lowabundance of MCK and sMitCK isoenzymes insarcoma, a much higher amount of protein had to beapplied compared with normal muscle. Detection of BCK and uMitCK isoforms in...
... polylepis).Calciseptineisaknown blocker of L-type Ca2+channels, a property thatunderlies its ability to block K+-induced contractions of aortic smooth muscle and spontaneous contractions of uterine smooth muscle [37]. ... specifically inhibits high K+-stimulated contraction of rat-tail arterial smooth muscle. (A) Blockage of high K+-induced contraction by ablomin. Smooth muscle strips were pretreated with1 lMablomin, ... inlatisemin). Contraction measurements on rat-tail arterial smooth muscle Helical strips of endothelium-free rat-tail arterial smooth muscle were prepared as described previously [41]. All thecontraction...
... of this paper was to investigate thepathophysiological roles of muscle- specific miRNAs inDM1, the most frequent autosomal dominant myopathyin adults. We therefore profiled the expression of ... pathohistological features of each DM1 specimens used in this study (sex, muscle, lysosomal activity and muscle pathohistological aspects). Muscle biopsies from VL of 1 female and 3 males have ... al., Overexpression of microRNA-206 in the skeletalmuscle from myotonic dystrophy type 1 patients Journal of Trans-lational Medicine 2010, 8:48 Gambardella et al. Journal of Translational Medicine...
... thebiomechanical properties ofskeletal muscles, even within the physiologic range of the excursion of the muscle- tendon unit.Published: 21 August 2007Journal of Orthopaedic Surgery and Research ... three-dimen-sional muscle model: a quantified relation between form andfunction ofskeletal muscles. J Morphol 1984, 182:95-113.19. Kaufman KR, An KN, Chao EYS: Incorporation ofmuscle archi-tecture ... Disruption of the muscle fibers occurredinitially, and disruption of the connective tissue occurredonly with large displacements of the muscle [34]. Ourresults are consistent with those of Kellis...
... aramp filter and a cutoff frequency of 0 .5 of the Nyquist fre-quency to obtain an image pixel size of 0.4 ì 0.4 ì 0.8 mmand an inter-plane spacing and slice thickness of 0.8 mmin a 128 × 128 ... administrationcorresponded to an increase in Kifor myocardium and skeletal muscle. Because skeletalmuscle constitutes a sig-nificant fraction of the body mass in mice [19], theobserved insulin-induced ... 1:8http://www.ejnmmires.com/content/1/1/8Page 8 of 9 ORIGINAL RESEARCH Open AccessInfluence of dietary state and insulin onmyocardial, skeletalmuscle and brain [18F]-fluorodeoxyglucose kinetics...