physiological and biochemical responses of chs1 chs2 and chs3 mutants to chilling
EFFECT OF STORAGE TEMPERATURES ON THE PHYSIOLOGICAL AND BIOCHEMICAL PROPERTIES OF HYLOCEREUS POLYRHIZUS
Ngày tải lên :
05/05/2014, 08:39
... Understanding the Composition and Complexity of Rural Households Agriculture and Human Values 22:87-103 Druiynska, B, A & Wotosiak, R 2007 The Influence of Time and Type of Solvent ,on Efficiency of ... M.R 1992 Effect of Food Preparation on Qualitative and Quantitative Distribution of Major Carotenoid Constituents of Tomatoes and Several Green Vegetables Journal ofAgricultural and Food Chemistry ... required to look into methods that were more applicable to all herbals but not methods for only a specific type of herb The outcome of this study was to provide drying and extraction parameters to...
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Physiological and biochemical responses to salt stress in the mangrove pptx
Ngày tải lên :
06/07/2014, 02:20
... into several segments Each segment was separated into xylem and cortex These tissues were put into tubes and centrifuged at 1300×g for h to obtain 25–65 l of sap from xylem and cortex of two to ... feature of mangroves is their ability to tolerate NaCl to seawater level (500 mM) From the physiological aspects, the effects of salinity on the photosynthesis of mangroves have been studied to some ... rate, and normalized to leaf area Each leaf was exposed for about 30 to each of a series of photon irradiances between and 2000 mol m−2 s−1 (4 sensor) For each salt concentration (0, 125, 250 and...
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Báo cáo lâm nghiệp: "Physiological and morphological responses of dormant and growing Norway spruce container seedlings to drought after planting" docx
Ngày tải lên :
08/08/2014, 00:21
... prolonged frozen storage instead of growing seedlings from the same seedling stock To achieve this aim, we compared physiological and morphological responses of dormant and growing seedlings to postplanting ... from –0.96 MPa to –2.22 MPa, indicating probably down-regulation of primary photochemistry and the augmentation of photoprotective mechanisms to avoid overreduction and photoinhibitory damage [6] ... influence of desiccation, rough handling and cold storage on the quality and establishment of Sitka spruce planting stock, Forestry 63 (1990) 129–141 [6] Eastman P.A.K., Camm E.L., Regulation of photosynthesis...
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Báo cáo lâm nghiệp: "Sexual reproduction in Populus I. Some physiological and biochemical events of the progamic phase" pdf
Ngày tải lên :
09/08/2014, 04:20
... related to a change in the physiology of the compatible pollen tube, shifting from an autotrophic to a heterotropic type of nutrition callose Biochemical studies focused on pollinic and stigmatic ... known to be involved in male-female interactions (Gaude and Dumas, 1987) After silver nitrate staining, qualitative and quantitative differences could be observed, related to the presence of P ... visualization of 15 stigmatic and pollinic glycoproteins according Differences to the glycoprotein type of increases were observed, distinct in the compat- cross: one only ible cross Moreover, f3-Galactosidase...
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Biochemical characterization of thermophilic lignocellulose degrading enzymes and their potential for biomass bioprocessing
Ngày tải lên :
05/09/2013, 16:11
... cellulase of Coptotermes formosanus had Km and Vmax values of 1.90 mg/ml and 148.2 U/mg protein, respectively, on CMC [65] Nakamura et al [66] reported a Km of 3.3 mg/ml CMC and a Vmax of 1100 ... 1.34 g/l) and xylose (up to 0.24 g/l) were fermented to ethanol by a recombinant E coli KO11 and similar ethanol yields were obtained (data not shown) Assimilation of both pentose and hexose ... Assistant Professor and was promoted to tenured Professor in 2006 He earned his B.S in Mathematics and Agricultural Engineering in India, a M.S degree in Food Engineering in Thailand, and completed...
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Tài liệu BIOCHEMICAL TARGETS OF PLANT BIOACTIVE COMPOUNDS A pharmacological reference guide to sites of action and biological effects doc
Ngày tải lên :
17/02/2014, 05:20
... of cytotoxic, irritant, inflammatory and toxic compounds from the Thymelaeaceae and the Euphorbiaceae Of particular note are the highly inflammatory PKC activators resirliferatoxin and tinyatoxin ... inhibits topoisomerase and binds to tubulin Podophyllotoxin-related compounds with antimitotic, cytotoxic and antitumour activity include 4'-demethylpodophyllotoxin, 4'demethyldeoxypodophyllotoxin and ... alkaloids are toxic and are of interest as anticancer and selective anti-protozoal agents because of their cytotoxicity Examples (some source genera in parentheses) include: the cytotoxic antimalarials...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
Ngày tải lên :
19/02/2014, 02:20
... the rate of tryptophan -to- NAD conversion Rat liver ACMSD gene expression is regulated by the two transcriptional factors: hepatocyte nuclear factor 4a (HNF4a) and peroxisome proliferator-activated ... amplification and cloning of ACMSD isoforms Human brain, liver and kidney total RNA (Clontech Laboratories, Inc., Mountain View, CA, USA) was reverse transcribed in the presence of random primers, ... verify incorporation of the desired modification and to ensure the absence of random mutations The mutagenized plasmids were transformed into P pastoris GS115 competent cells and expression was...
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Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx
Ngày tải lên :
19/02/2014, 06:20
... anisotropy of the free minifiber, AB is the anisotropy of the minifiber bound to the penton base, [LigB] is the concentration of the bound ligand (minifiber), and [Ligtot] is the total concentration of ... expression, the SF21 and Hi5 cell lines and the Bac -to- Bac expression system and vectors were from Invitrogen (Carlsbad, CA, USA) Protease inhibitors were from Roche (Basel, Switzerland) and Ni-NTA resin ... fractions of interest were then concentrated and stored at ) 80 °C TMR labeling and binding assays A five-fold excess of malemide-derivatized TMR was added to a solution of purified minifiber and allowed...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
Ngày tải lên :
19/02/2014, 07:20
... column and the mass spectrometer was used, and 100 lLÆmin)1 of eluent was introduced into the mass spectrometer A LCT TOF (time -of- flight) mass spectrometer (Micromass) was used in the negative and ... glutaconyl-CoA and HMG-CoA as well as crotonyl-CoA, 3-hydroxybutyryl-CoA and 3-methylcrotonyl-CoA were used for the kinetic characterization of AUH The data are summarized in Table Overexpression of a ... group of the CoA-acceptor (R2-COO–) In the case of glutaconate CoA-transferase from A fermentans, CoAS– transiently is bound to the c-carboxyl group of bE54 [24] Mutations in AUH are linked to the...
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc
Ngày tải lên :
19/02/2014, 13:20
... putative orthologs of the type I isomerase Highest degrees of similarity were found to Idi-1 proteins of the c-proteobacteria A vinelandii and P luminescens and to Idi-1 protein of the Bacteroid ... to glycolate oxidase Secondary structures and sequence numbers refer to gox (top lines) and idi (bottom lines), respectively The eight ba modules of the TIM-barrel domain are colored yellow and ... IPP or DMAPP, respectively, and 0.2 mg of enzyme per mL), the conversion of IPP into DMAPP and vice versa approached a state of equilibrium after a reaction period of about h (Fig 5) The rates...
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Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf
Ngày tải lên :
22/02/2014, 07:20
... difficult to assign spectra of CR I–II at lower pH due to the overlap of broad resonances When the pH was lowered to pH 6.7, it was possible to assign most of the resonances between L13 and F98 Many of ... contrast to Calb I –II, CR I–II shows no tendency to dimerize and both EF-hands of CR I–II bind calcium We conclude that the significant structural and biochemical differences between CR I –II and ... Calcium-binding to the CR II EF-hand motif Samples of GST, CR, GST–CR I, GST –CR II and standards were separated on 10% acrylamide Tris/tricine/gels in the presence of SDS and then electroblotted to nitrocellulose...
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Báo cáo khoa học: Proteomic and biochemical analysis of 14-3-3-binding proteins during C2-ceramide-induced apoptosis pot
Ngày tải lên :
06/03/2014, 22:21
... protein ⁄ ALG2 (apoptosis-linked gene 2), second mitochondrial-derived activator of caspase (Smac), signal transducer and activator of transcription (STAT3), and hematopoietic progenitor kinase 1-interacting ... regulators of apoptosis, such as RIP1, Smac, STAT3, and HIP-55, were previously identified as 14-3-3-interactor proteins by a combination of 14-3-3affinity chromatography purification and MALDI-TOF ... percentage of breast tumors in the early stage of the disease, contributing to the transformation of cells and also to the further progression of breast cancer [42] On the other hand, downregulation of...
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Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx
Ngày tải lên :
07/03/2014, 05:20
... view of USP7 shown below by acetylation, as confirmed by MALDI-TOF-MS LC-MS analysis of USP7-FL revealed two protein masses of 130 464.0 and 130 540.0 Da, corresponding very likely to acetylated and ... at T36 and extending to residue L49, and a larger helix including amino acids A55 to R66 Based on these predictions, we studied the localization of deletion mutants of the first 20, 50 and 70 ... amino acids 1–205, 20–205, 50–205 and 70–205 were amplified with BamHI and AgeI 5¢- and 3¢-overhangs, ligated into pCR2.1-TOPO and finally subcloned into the vector pEGFPN1 (Clontech, EMD Biosciences)...
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Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt
Ngày tải lên :
07/03/2014, 17:20
... fructose and galactose Amoebal HK isoenzymes are strongly inhibited by AMP and ADP, but glucose 6-phosphate (Glc6P), the potent modulator of some mammalian HK enzymes [16], is a weak inhibitor of ... with a higher concentration of PGAM than of PPDK at pH The control analysis showed CJ Ei values of 0.08 and 0.2 for PGAM and 0.85 and 0.57 for PPDK at 10 and 39 mU of added PPDK, respectively ... metal cofactor and are present in bacteria, protozoa, animal and plant cells [34] This analysis [26] indicates that EhALDO belongs to the class II aldolases, whereas EhPGAM can be grouped together...
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Báo cáo Y học: Purification and biochemical characterization of some of the properties of recombinant human kynureninase pptx
Ngày tải lên :
08/03/2014, 22:20
... both SDS/PAGE and MALDITOF MS of the whole protein Both purified and crude samples of recombinant kynureninase can be stored in the absence or presence of PLP for extended periods of time (> 12 ... absence of reducing agents SDS and mercaptoethanol to determine the native dimeric molecular mass resulted in the appearance of two bands at 52.5 and 95 kDa (gel image not shown), and this ... (1993) Chemistry and neurochemistry of the kynurenine pathway of tryptophan metabolism Chem Soc Rev 45, 309–315 Stone, T.W (2000) Development and therapeutic potential of kynurenic acid and kynurenine...
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Báo cáo Y học: Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungfish (Lepidosiren paradoxa) docx
Ngày tải lên :
08/03/2014, 22:20
... Pietro and J A Santome (Eur J Biochem 269) Preparation of the lungfish liver cytosolic fraction and purification of calhepatin Livers were excised from L paradoxa weighing 600–700 g, cut into small ... of the cytosolic fraction from lungfish and rat tissues Tissues were cut into small pieces, suspended in 40 mM sodium phosphate (pH 7.4) containing 150 mM KCl, mM EDTA and mM dithiothreitol, and ... DEAE-cellulose chromatography, and gel filtration on a Superdex 200 column They were then concentrated with the Centriprep concentrator up to a concentration of 10 mgÆmL)1 and stored at )40 °C Ó...
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Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc
Ngày tải lên :
15/03/2014, 10:20
... activity, with Hill coefficients of 2.4 and 1.9 and dissociation constants (KdHill) of and lm for hGBP5a ⁄ b and hGBP5ta, respectively The proteins are very similar in terms of their enzymatic activity, ... the lack of GMP formation (see Fig S2) The similar hydrolytic activities of the a ⁄ b and C-terminally truncated forms of hGBP5 are in contrast to that of the analogous deletion mutant of hGBP1 ... 1601 Biophysical properties of hGBP5a ⁄ b and hGBP5ta M Wehner and C Herrmann (kdiss ) in Fig S3 are similar, and show a five- to off 10-fold lower rate for dissociation of mant-GDP (0.129 ⁄ 0.23...
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