physical processes and enzymatic modification

Proteins in food processing

Ngày tải lên : 23/05/2014, 13:34

... 4.4 Improving functionality of whey proteins in foods: physical processes and enzymatic modification 4.5 Sources of further information and advice 4.6 References Muscle proteins Y L Xiong, University ... general (e.g., Branden and Tooze, 1999; Creighton, 1993; Darby and Creighton, 1993; Lesk, 2001) and in the specific context of food proteins (e.g., Hettiarachchy and Ziegler, 1994; Nakai and Modler, ... N K (1999), `Chemical and enzymatic modifications', in Nakai S and Modler H W, Food Proteins Properties and Characterization, New York, VCH, 235±280 HOWELL N K, HERMAN H and LI-CHAN E C Y (2001),...

643
1.6K
0

Báo cáo khoa học: Protein and mRNA content of TcDHH1-containing mRNPs in Trypanosoma cruzi pdf

Ngày tải lên : 15/03/2014, 23:20

... Dhh1 (yeast) and Rck ⁄ p54 (mammals) proteins [14] It has been reported that Dhh1 is involved in multiple RNA-related processes in various eukaryotes, and accumulates in stress granules and P-bodies ... Benchmark Protein Ladder (Gibco, Grand Island, NY, USA) the supernatant (SP) were resolved by SDS ⁄ PAGE and visualized by silver staining (Fig 3A) The IP and SP samples obtained with pre-immune ... and proteins from the supernatant fraction (SP) were resolved by SDS ⁄ PAGE and visualized by silver staining (D) Western blot analysis of IP and SP samples, obtained with pre-immune serum, and...

12
481
0

Báo cáo khoa học: Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identiﬁcation of kinase substrates doc

Ngày tải lên : 16/03/2014, 18:20

... extract (lanes and 5), cytosolic fraction (lanes and 6) and membrane fraction (3 and 7) of S pneumoniae strains Puriﬁed recombinant StkP was used as a control (lane 4) Arrows indicate bands corresponding ... PAGE, and radioactive bands revealed by autoradiography Positions and molecular mass (kDa) of protein standards are indicated on the left The arrows indicate the position of phosphorylated rStkP and ... mutation and a silent NaeI site) and PMUT2 (which introduced the D231A mutation and a silent StuI site) and reverse primer PHPP-R in the ﬁrst round of PCR The generated fragments (190 and 75 bp,...

12
466
0

Báo cáo lâm nghiệp: " Growth and mineral content of young chestnut trees under controlled conditions of nutrition" ppsx

Ngày tải lên : 08/08/2014, 18:22

... supplied (C and D) in September1994 And a decreasing trend of K concentrations occurred under O and A between January and September 1995 and under C and D between September 1994 and September ... of a 2:6:6 (v/v/v) mixture of H HNO and H according O , SO 23 Concento Hoenig and Vanderstappen trations of Ca, Mg and Mn were determined by atomic absorption and K contents by atomic (1978) emission ... value was four times greater in C and D, and intermediate in B trees compared to O and A trees pH substratum evolution Before both fertilization treatments and the pH of the substratum was 5.1...

13
261
0

Báo cáo khoa học: "gelling agents on growth, mineral composition and naphthoquinone content of in vitro explants of hybrid walnut tree (Juglans regia x Juglans nigra)" pdf

Ngày tải lên : 08/08/2014, 23:22

... abovementioned factors al, 1989) As result of their physical and chemical properties, gelling agents influence growth (Lee et al, 1986; Cornu and JayAllemand, 1989) and organogenesis (Titel et al, 1987; Koda ... juvenile-leaf resistance to anthracnose and Clines S, Jay-Allemand C, Drouet A, Ouaras A, Cornu D (1989) Polyphenolic and enzymatic characterization of ageing and rejuvenation of walnut trees (Juglans ... ageing (Cline and Neely, 1984) Moreover, accumulation of phenolic compounds was associated with deficient mineral nutrition and stress (Di- MATERIALS AND METHODS Tissue culture and growth conditions...

10
329
0

Báo cáo y học: "The protective effect of licofelone on experimental osteoarthritis is correlated with the downregulation of gene expression and protein synthesis of several major cartilage catabolic factors: MMP-13, cathepsin K and aggrecanase" docx

Ngày tải lên : 09/08/2014, 06:23

... MB, JB, FM, CG and JMP performed the acquisition of data JPP, CB, FM, PR, DL and JMP analyzed and interpreted the data JPP, CB, PR, DL and JMP prepared the manuscript JPP, CB, JB and JMP performed ... collected and processed with GeneAmp® 5700 SDS software and given as a threshold cycle (Ct) Plasmid DNA containing the target gene sequences was used to generate standard curves A DNA standard curve ... are expressed as median and range and are presented as box plots, where the boxes represent the 1st and 3rd quartiles, the line within the box represents the median, and the lines outside the...

12
1.2K
0

báo cáo khoa học: " Effect of filtration on morphine and particle content of injections prepared from slow-release oral morphine tablets" ppt

Ngày tải lên : 11/08/2014, 18:20

... reversed-phase column and UV detection at 286 nm The mobile phase was 90% phosphate buffer (50 mM, pH 3) and 10% methanol, and flow rate 0.7 ml/min Injections were made using a 20 μl loop Standard solutions ... the photomicrographs, and to Tania Hunt from the Tasmanian Council on AIDS, Hepatitis and Related Diseases for assisting with organisation of consumer interviews and surveys, and to the consumers ... the particle counting, and drafted the manuscript RB conceived of the study, participated in its design and coordination and helped to draft the manuscript SB developed and conducted the morphine...

13
352
0

Báo cáo y học: " Elevated expression of both mRNA and protein levels of IL-17A in sputum of stable Cystic Fibrosis patients" potx

Ngày tải lên : 12/08/2014, 13:22

... colonisation state of P aeruginosa (A and B) (never/free/intermittent: n = 17 and chronic: n = 21) and S aureus (never/free/intermittent: n = 22 and chronic: n = 16) (C and D) according to the Leeds ... Diagnostics, Düdingen, Switzerland) Per slide, 250 leukocytes and epithelial cells were counted and percentages of inflammatory cells (macrophages, neutrophils, eosinophils and lymphocytes) were calculated ... = 11) and CF patients (n = 38) Median (P25-P75) IL-17A mRNA levels were 0.14 (0.06-0.95) for controls and 6.1 (2.6-11.6) for CF and median IL-23 mRNA levels 2.4 (0.25-6.2) for controls and 19.3...

8
288
0

Timing and information content of insider trades, before and after the sarbanes oxley act of 2002

Ngày tải lên : 30/09/2015, 17:19

... returns and trading volumes, and I control for the information environment around the disclosure As demonstrated theoretically by Karpoff (1986), Kim and Verrechia (1991), and Dontoh and Ronen ... - before and after SOX 68 Table 9: Returns and volumes around SEC filing dates of insider trades, in three periods: pre- and post-Enron bankruptcy, post-SOX 69 Table 10: Reporting lag and information ... irrespective of book-to-market ratio and size (about 0.13% for purchases and -0.23% for sales) Aboody and Lev (2000) find more positive (negative) raw returns and higher trading volumes following...

94
334
0

Báo cáo sinh học: "Genetic parameters for lactation traits of milking ewes: protein content and composition, fat, somatic cells and individual laboratory cheese yield" ppt

Ngày tải lên : 14/08/2014, 13:21

... yield and fat content and a nonsignicant effect on protein fractions, LSCC and LILCY This result agrees with others on Churra [12,17,20] and Latxa [21] ewes for fat and protein contents and somatic ... to affect milk yield and composition in the Churra breed conditions [11] There were four lambing age groups (1: between and yrs; 2: between and yrs; 3: between and yrs; and 4: over yrs), two ... count, fat and protein in dairy sheep, J Dairy Sci 77 (1994) 15371542 [25] Gonzalo C., Microbiological and hygienic quality of ewe and goat milk: somatic cells and pathogens, in: Production and Utilization...

16
316
0

Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"

Ngày tải lên : 25/10/2012, 11:10

... adiponectin and the C-terminus of GLP-1-A Compared with native GLP-1, the fusion protein has a modified site and larger molecular size, and may circumvent the rapid inactivation and renal clearance ... resistance, type diabetes, and cardiovascular disease Diabetes 2007; 5: 1198-209 Hu XB, Zhang YJ, Zhang HT, et al Cloning and expression of adiponectin and its globular domain, and measurement of the ... weight and small molecular size, and most of them may not connect with plasma albumin [15] These characteristics facilitate the filtration of GLP-1 through kidney [15] Although structural modification...

7
612
0

Starch and Protein Quality Requirements of Japanese Alkaline Noodles (Ramen).pdf

Ngày tải lên : 16/12/2012, 15:25

... analyses included FN tests on the 20 wheat samples, and protein and ash determinations on the flours MATERIALS AND METHODS Grain and Flour Samples and Subsequent Treatments Experiment Determination ... (1992) and modified by Crosbie and Lambe (1993) Fig Relationship between flour swelling volume (FSV) assessed in water and total texture score TABLE I Means, Standard Deviation (SD), and Coefficients ... Z., and Pomeranz, Y 1994 Role and contribution of starch and protein contents and quality to texture profile analysis of oriental noodles Cereal Chem 71:315-320 Batey, I L., Curtin, B M., and...

7
978
4

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Ngày tải lên : 15/02/2014, 01:20

... directly, and therefore it is a more accurate and sensitive method compared to the spectrophotometric methods Because the enzymatic activity of PRTFDC1 towards hypoxanthine and guanine is only 0.26% and ... Environment, Agricultural Sciences and Spatial Planning (to L.W and S.E.), the 4928 Swedish Research Council (to H.E and P.N.) and the Swedish Cancer Foundation (to H.E and P.N.) The Structural Genomics ... PRTFDC1 (A) monomer and (B) tetramer generated using symmetry-related molecules Interactions with GMP (C) GMP, phosphate and calcium ions are shown as sticks and spheres colored pink and black, respectively...

11
770
0

Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

Ngày tải lên : 18/02/2014, 14:20

... lanes 2,3 and 4, lower band) The levels of mutant and wild-type MCPIP mRNAs were higher when these vectors were co-transfected with a vector overexpressing IL-1b (Fig 5, lanes and 4, upper bands) ... intermediate levels in heart and placenta, and the lowest levels in spleen, kidney, liver and lung The transcript was not detectable in brain, thymus, muscles, small intestine and colon (Fig 3A) The ... within the PIN domain (D141A and D226A) Transcription was inhibited by addition of actinomycin D, and RNA was collected after 3, and h The mRNA level for IL-1b (top panel) and MCPIP (bottom panel)...

14
598
0

Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc

Ngày tải lên : 19/02/2014, 12:20

... Hoff analysis and those determined by uorescence and DSC (see before) Determination of the ThÂ and TsÂ The values of the temperatures where the enthalpy and entropy are zero, ThÂ and TsÂ, respectively, ... respectively; and at 318 K, 33 kcalặmol)1 and 4.4 3.9 kcalặmol)1ặM)1, respectively; (B) at 293 K, 3.7 3.3 calặmol)1ặK)1 and 0.7 calặ mol)1ặK)1ặM)1, respectively; and, at 318 K, 98 11 calặmol)1ặK)1 and ... stability of scHPr (namely, DS, DH and DCp), using different biophysical techniques [uorescence, circular dichroism (CD) and differential scanning calorimetry (DSC)] and to compare these with the...

17
612
0

Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt

Ngày tải lên : 19/02/2014, 13:20

... hindrance between peptide and protein, and to relax the hydrogen added to the protein, the peptide and protein were merged and minimized before docking Atomic solvation parameters and fragmental volumes ... temperature probe The one- and two-dimensional NMR experiments were performed and processed on 300 MHz and 500 MHz Bruker DRX spectrometers equipped with a 5-mm broad-band inverse probe, at a proton ... Intensities were assigned as strong, medium and weak with upper and lower boundaries of distance for dNa (i, i), daN (i, i +1) and dNN (i, i +1), 1.9–3.0, 2.2–3.6 and ˚ 3.0–5.0 A, respectively [23] Side...

14
657
0

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

Ngày tải lên : 19/02/2014, 18:20

... speciﬁc for human Ras-GRF1 Primer pairs: lane 2, ON359 and ON360; lane 3, ON357 and ON358; and lane 4, ON361 and ON360 The PCR products and a DNA size marker, lane 1, were separated on agarose ... increase and show Ca2+ dependence of serotonininduced ERK1 ⁄ phosphorylation and a mandatory role of the Ca2+ ⁄ CaM-binding IQ domain in RasGRF1-stimulated ERK1 ⁄ phosphorylation Thus, both cAMP and ... that the treatment of NIH3T3 and COS-7 cells with carbachol [9] and lysophosphatidic acid [11], activating both Gq- and Gi-coupled receptors, induces the activation and phosphorylation of Ras-GRF1...

13
730
0

Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by ﬂuorescence spectroscopy pdf

Ngày tải lên : 21/02/2014, 01:21

... is sensed by the P-pyridoxyl group, and allowed the acquisition of detailed and reliable information on the binding of several ligands and on the role of Mn2+ and Mg2+ on their binding The comparison ... saturating concentration of ligand, P the total protein concentration, and L is referred to the ligand concentration The distribution of metal ion as [M2+]free, [ML] and of [L]free was calculated ... (MSI) and force eld CVFF and ESFF (MSI), that has all parameters needed for the octahedral Mn2+ coordination and amino acids (1AQ2) This program was also employed for energy minimization and molecular...

9
533
0

Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx

Ngày tải lên : 21/02/2014, 03:20

... N-Ac-Trp-OMe, Gly-Tyr-Gly, Gly-His-Gly, lysozyme and RNase A were incubated with GAPDH, GR, and LDH, in the presence and absence of added Fe2+EDTA and the residual enzymatic activity determined (Fig 4, ... H2O2 and peptide and protein peroxides after photo-oxidation with 1O2 generated by rose bengal in the presence of visible light and oxygen (a) RNase A (50 mgặmL)1); (b) N-Ac-TrpOMe (2.5 mM); and ... peptide and protein peroxides investigated in the current study, with GAPDH being more sensitive than GR and LDH, and inactivation of GAPDH being metalion independent, whereas inhibition of GR and...

10
462
0