phyllanthus for its anti cancer properties
PULMONARY HYPERTENSION – FROM BENCH RESEARCH TO CLINICAL CHALLENGES pot
... processed for immunofluorescence as previously described (Arciniegas et al., 2005) using the following antibodies: a mouse monoclonal antibody (mab) anti- human integrin β3 (GPIIIa, CD61) and a mab anti- chicken ... polyclonal antibody (pab) phospho-FAK (Tyr397) (Sigma-Aldrich) Negative controls were performed by omitting the primary antibody incubation step or by using non immune serum in place of primary antibody ... caveolae, rich in cholesterol and sphingolipids that serve as a platform for a numerous signaling molecules and compartmentalize them for optimum function Caveolin-1, a major protein constituent of...
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Báo cáo y học: " Involvement of mast cells in monocrotalineinduced pulmonary hypertension in rats" pot
... double-staining the sections with an anti- a-smooth muscle actin antibody (dilution 1:900, clone 1A4, Sigma, Saint Louis, Missouri) and anti- human von Willebrand factor antibody (vWF, dilution 1:900, ... morphometry were performed as described [27] The formalin-fixed and paraffin-embedded lung tissues were subject to sectioning to yield μm thick sections Elastica staining was performed according ... was performed using standard protocols Briefly, paraffinembedded tissue sections were dewaxed, rehydrated and incubated with 0.05% w/v toluidine blue for 2-3 minutes MC density was quantified...
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Báo cáo y học: "Inhaled nitric oxide in persistent pulmonary hypertension of the newborn refractory to high-frequency ventilatio" ppsx
... oxygen concentration was analyzed continuously before it reached the neonate’s endotracheal tube Nitric oxide was initially administered at 20 ppm for at least h If there was no response while the ... potential of HFOV to reduce the need for extracorporeal membrane oxygenation (ECMO) A number of reports identify a number of infants who, although referred for ECMO, survived without using this ... [12–14] In this study ECMO was not used as an alternative therapy for the INO non-responders because it was not available in our hospital for neonates It has been observed that some infants who showed...
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Báo cáo y học: "Prophylactic antibiotic treatment is superior to therapy on-demand in experimental necrotising pancreatitis" pptx
... strategies for the treatment of acute pancreatitis [7,18,19] To date, it remains unclear whether prophylactic antibiotic treatment or on-demand antibiotic therapy is more beneficial Therefore, we ... significantly by early prophylactic antibiotic treatment but not delayed antibiotic treatment on-demand • Prophylactic antibiotic treatment is superior to treatment on-demand Thus, antibiotics are recommended ... number not for citation purposes) Available online http://ccforum.com/content/12/6/R141 did not show any bacterial infection In these experiments, all animals that received prophylactic antibiotics...
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Báo cáo y học: " Effects of inhaled iloprost on right ventricular contractility, right ventriculo-vascular coupling and ventricular interdependence: a randomized placebo-controlled trial in an experimental model of acute pulmonary hypertension" ppt
... used the current 'gold standard' methods to quantify biventricular contractile performance and cardiac loading conditions in an experimental model for acute PHT as well as in healthy animals with ... ± 0.06 7.47 ± 0.04 Values are shown for baseline and in pulmonary hypertension before inhalation and minutes after inhalation of either iloprost or control For the complete experimental time course, ... obviates the need for homeometric autoregulation and allows the right ventricle to return to its baseline (lower) contractile state RV, right ventricular Ventricular afterload was quantified as effective...
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báo cáo hóa học: " Development and validation of a preference based measure derived from the Cambridge Pulmonary Hypertension Outcome Review (CAMPHOR) for use in cost utility analyses" doc
... subdivided as follows: For states better than being dead, the value of the health state x/t is bounded by 1.0 for perfect health and zero for states as bad as being dead For states worse than ... is the mean TTO value for each health state and x is a vector of dummy explanatory variables (x∂λ) for each level λ of dimension ∂ of the simplified CAMPHOR classification For example, x31 denotes ... λ = (I don't feel very dependent) For any given health state x∂λ will be defined as follows: x∂λ = 1, if for this state dimension ∂ is at level λ x∂λ = 0, if for this state, dimension ∂ is not...
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Báo cáo y học: "Cold hands — strained heart? Advances in the management of Raynaud’s phenomenon and pulmonary hypertension" ppt
... management approach for SScl vasculopathy is outdated and that the widespread use of calcium channel antagonists for RP is probably inappropriate It now appears preferable to use agents for which there ... Functional Class II are awaited The combined vasodilator/antiproliferative/ antifibrotic activity of ERAs raises the exciting prospect for management of peripheral vasculopathy in SScl Last year, the RAPIDS-1 ... them attractive candidates for treating RP Case reports are encouraging but controlled trials are required These approaches depart from the previously dominant strategy for both PAH and RP, which...
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Báo cáo y học: "Role of chymase in cigarette smoke-induced pulmonary artery remodeling and pulmonary hypertension in hamsters" doc
... The PCR program for chymase was initiated by a denaturation step at 94°C, followed by 35 cycles of 94°C for 30 s, 63°C for 30 s and 72°C for min, and a final extension at 72°C for PCR products ... difluoride (PVDF) membranes (Millipore, Bedford, USA) Primary antibodies used were chymase monoclonal antibody (1:1000; Chemicon, Temecula, USA), TGF-b1 polyclonal antibody (1:500; Santa Cruz Biotechnology, ... chymase PCR (738 bp) were (forward) 5’CTG AGA GGA TGC TTC TTC CTG C-3’ and Page of 10 (reverse) 5’-AGA TCT TAT TGA TCC AGG GCC G-3’ [23] Primers for b-actin PCR (194 bp) were (forward) 5’-CCT GTA TGC...
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Báo cáo y học: " Beta-estradiol attenuates hypoxic pulmonary hypertension by stabilizing the expression of p27kip1 in rats" ppsx
... 95°C for 30 s, 59°C for 30 s and 72°C for min, and a final extension at 72°C for 10 min; and for b-actin was began with a denaturation step at 95°C, followed by 35 cycles of 95°C for 30 s, 55°C for ... program for p27kip1 was started by a denaturation procedure at 95°C, followed by 35 cycles of 95°C for 30 s, 51°C for 30 s and 72°C for min, and a final extension at 72°C for 10 min; for Skp-2 ... nitrocellulose filter membranes (Millipore, Bedford, USA) The primary antibodies were p27kip1 antibody (1:1000; Millipore, Bedford, USA), Skp-2 polyclonal antibody (1:50; Abcam, Cambridge, UK) and...
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Báo cáo y học: " Effect of chemokine receptor CXCR4 on hypoxia-induced pulmonary hypertension and vascular remodeling in rats" pps
... cells were stained with primary antibodies, anti- mouse/rat CD34 (R&D Systems, Inc Minneapolis, MN) and anti- rat CD45 (BioLegend, San Diego, CA) Following incubation for 30 minutes and washing with ... rat lung: (A) GFP expression Proteins were isolated form rat lungs and Western blot was performed for analysis of GFP protein expression Quantitative data (upper panel), setting hypoxia BMC as ... groups (B) c-kit expression Proteins were isolated form rat pulmonary artery and Western blot was performed for analysis of c-kit expression Quantitative data (upper panel), setting normoxia control...
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Báo cáo y học: " Protective effects of hydrogen-rich saline on monocrotaline-induced pulmonary hypertension in a rat model" potx
... Moreover, it has been reported that hydrogen-rich saline has an antiinflammatory effect [13] Therefore, we hypothesized that the antioxidant and anti- inflammatory effects of hydrogen-rich saline might ... with its anti- oxidative effect Conclusions This study shows that hydrogen-rich saline treatment ameliorates the progression of PH induced by MCT in rats, which may be associated with its anti- inflammatory ... opened, blood was taken from the heart for serum preparation, and lung and heart were processed for histological evaluation or frozen in liquid nitrogen for further analysis Drugs and materials...
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Báo cáo y học: " Adrenomedullin in inflammatory process associated with experimental pulmonary fibrosis" pot
... PBS, v/v), anti- iNOS antibody (1:500 in PBS, v/v), antiP-selectin antibody (BD Pharmingen, CD62P 1:500), anti- ICAM-1 antibody (BD Pharmingen, CD54, 1:500), anti- nitrotyrosine antibody (1:500 in ... incubation for 15 with biotin and avidin (DBA, Milan, Italy), respectively Sections were incubated overnight with anti- TNF-a antibody (1:500 in PBS, v/v), anti- IL-1b antibody (1:500 in PBS, v/v), anti- iNOS ... in PBS, v/v), or PAR antibody (1:500 in PBS, v/v) and anti- TGF-b rabbit polyclonal antibody (1:500 in PBS, v/v) Sections were washed in PBS and incubated with secondary antibody Specific labeling...
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Báo cáo y học: " Therapeutic efficacy of TBC3711 in monocrotaline-induced pulmonary hypertension" pot
... staining and (b) double immunostaining (anti- a-smooth muscle actin (purple/violet color) and anti- von Willebrand factor (brown color) antibodies) were performed to assess medial wall thickness ... measures of pulmonary vascular remodelling Immunostaining with anti- proliferating cell nuclear antigen (PCNA) antibody (c) was performed to assess the in situ proliferation state of pulmonary ... by echocardiography are shown for all experimental groups Bars represent mean ± SEM One-way ANOVA with Newman-Keuls multiple comparison post-hoc test was performed for statistical analysis ***P...
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Báo cáo y học: " Endothelial-like cells in chronic thromboembolic pulmonary hypertension: crosstalk with myofibroblast-like cells" pptx
... Willebrand factor, anti- vimentin and anti- desmin) for hour at room temperature, and then with secondary antibodies (antimouse IgG conjugated with Alexa-594 fluorescent dye and anti- rabbit IgG conjugated ... acetone for minutes followed by blocking with normal goat serum for 30 minutes as described previously [13] The cells were incubated with primary antibodies (antia-smooth muscle actin (SMA), anti- von ... immunofluorescence staining using anti- von Willebrand factor (Figure 3D), anti- a-SMA (Figure 3D), anti- vimentin (data not shown), and antihuman desmin (data not shown) antibodies The endothelial cell-specific...
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Báo cáo y học: "Impact of interleukin-6 on hypoxia-induced pulmonary hypertension and lung inflammation in mice" ppt
... major biomarker for cardiovascular risk and the main stimulant for hepatic production of C-reactive protein, a compound widely used as a biomarker for atherosclerosis [13] A role for IL-6 in the ... provided Primers used for detecting RNAs for IL-6, sIL-6-R, gp130, ET-1, MCP-1, ICAM, and VCAM in the lungs are listed in table For each sample, the amplification reaction was performed in duplicate ... incubated with rat anti- mouse F4/80 antibody (diluted 1:1000; Abd Serotec) at 4°C overnight with rocking The membrane was then incubated with secondary anti- rat antibody for h at room temperature After...
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Báo cáo y học: " Nitric oxide synthases in infants and children with pulmonary hypertension and congenital heart disease" ppsx
... pressure cooking antigen retrieval; nNOS: catalogue reference 610308, BD Biosciences, UK, used at 1:400 dilution along with trypsin antigen retrieval) Bound antibody was detected using goat anti- mouse ... = mild; = moderate Separate quantification was performed for eNOS in small artery endothelium, small artery media, respiratory epithelium, alveolar macrophages Antibody dilutions were chosen ... and then treated for antigen retrieval by microwave pressure cooking or trypsin incubation The sections were then treated to block non-specific binding of primary and secondary antibodies and non-specific...
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Báo cáo y học: "Collagen V-induced nasal tolerance downregulates pulmonary collagen mRNA gene and TGF-beta expression in experimental systemic sclerosis" doc
... the presence of types I, III and IV anti- collagen antibodies, circulating immune complexes, and the emergence of antinuclear antibodies (ANA) and anti- Scl-70 antibodies [15] Based on Sakkass works ... each, with PBS and incubated with type I or V anti- collagen mouse polyclonal antibody, diluted at 1:50 in PBS, and type III anti- collagen monoclonal antibody (Calbiochem), with a 1:50 dilution ... method (CT) was used for the quantification of gene Table Sequence and description of the genes selected for the study Gene Genbank Accession Number Primer sense (5-3) Primer antisense (5-3) Product...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008