permeability of lipid membranes
Báo cáo khoa học: Interaction of ostreolysin, a cytolytic protein from the edible mushroom Pleurotus ostreatus, with lipid membranes and modulation by lysophospholipids pptx
Ngày tải lên :
23/03/2014, 21:20
... reproduce pore formation in articial membranes formed of erythrocyte total lipid extracts, suggesting the presence of a specic lipid acceptor(s) When the ability of lipids to inhibit ostreolysin-mediated ... absence of lipids (Fig 4), further evidence for a specic interaction We then assayed a series of pure lipids, or lipid mixtures, for ostreolysin inhibition (Table 1) None of the fully acylated lipids ... 90 (with respect to the plane of reections) In the case of lipid- bound protein, the spectrum of the protein was obtained by subtracting the contribution of the lipid alone, with a weight that...
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Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 1
Ngày tải lên :
14/09/2015, 21:54
... different lipid monolayers 7.3.2.4 Effect of lipid packing on the penetration of V4 7.3.3 AFM studies of antimicrobial peptides interacting with lipid monolayers 7.3.3.1 AFM images of pure lipid ... Titration of 100 nM V4-TMR with increasing concentrations of LPS 89 Figure 5.5 Comparison of ACFs of V4-TMR and complexes of V4-TMR with LPS, lipid A and PC 91 Figure 5.6 Comparison of V4-TMR ... energy of lipid monolayers incorporated with different percentage of V4 153 Figure 7.6 Compressibilities of lipid monolayers incorporated with different percentage of V4 154 Figure 7.7 Kinetics of...
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Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 3
Ngày tải lên :
14/09/2015, 23:32
... conformation of lipid through peptide -lipid interaction and imposed a surface pressure increase effect on the lipid monolayer 7.3.2 Penetration studies of antimicrobial peptides interacting with lipid ... lipid monolayers The interaction of V4 with different lipid monolayers was investigated to examine the penetration ability of V4 into different membranes Fig 7.11 illustrates the comparison of ... and POPC isotherms, indicative of the negligible effect of electrostatic interaction 145 Fig 7.1 Isotherms of POPG and POPC monolayers 7.3.1.2 Isotherms of mixed lipid/ V4 monolayers Fig 7.2 shows...
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Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 2
Ngày tải lên :
14/09/2015, 23:32
... mixed lipids SUVs complex was similar to that of pure lipids SUVs, between 0.6 and ms Fig 5.7 Binding of V4-TMR to SUVs of mixed lipid composition Shown are ACFs of V4-TMR bound to SUVs made of ... exhibited an F2 of 4.2 % and an increase in the fluorescence yield compared to TMR of Q2/Q1 of 3.21 90 Fig 5.5 Comparison of ACFs of V4-TMR and the complexes of V4-TMR with LPS, lipid A and PC ... The binding efficiency of V4-TMR with lipid A was lower than that of LPS despite the fact that lipid A is considered to be the bioactive part of LPS 99 5.4.4 Saturation of lipids affects the binding...
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A capillary-based method determining the permeability of sand layer for geothermal applications
Ngày tải lên :
05/09/2013, 17:03
... respectively He is a professor of School of Energy and Environment, Hebei University of Technology Since from 2005, he has taken as the dean of School of Energy and Environment of HBUT His work focuses ... properties of sands and gravels, with Special Reference to their Use in Filtration 24th Annual Report of Massachusetts State Board of Health, 1892, 34,539-556 [5] Shepherd RG, Correlations of permeability ... associate professor of School of Energy and Environment, Hebei University of Technology His work focuses mainly on heat and mass transfer related with renewable energies, HV&AC, and energy saving of buildings...
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The permeability of class boundaries
Ngày tải lên :
01/11/2013, 07:20
... the static permeability of ethnic boundaries, while the problem of ``salad-bar ethnicity'' and the intergenerational transmission of ethnicity are aspects of the dynamic permeability of those boundaries ... are instances of the static permeability of the boundaries of academic disciplines, while the pattern of career trajectories through academic specialities is an example of dynamic permeability ... the problem of the permeability of social boundaries is by no means restricted to class analysis International migration, for example, constitutes an aspect of the dynamic permeability of national...
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Báo cáo khoa học: Detailed structure of lipid A isolated from lipopolysaccharide from the marine proteobacterium Marinomonas vaga ATCC 27119T pot
Ngày tải lên :
07/03/2014, 15:20
... [23] Isolation of lipid A To obtain lipid A, LPS (300 mg) was hydrolysed in 1% (v/v) aqueous acetic acid for h at 100 °C Chloroform solution of the sediment obtained by centrifugation of the LPS ... peaks of equal intensity at m/z 1281.39 and 1253.67 as the highest mass ions, demonstrating the presence of two molecular forms of the lipid A studied Characterization of the fatty acid moiety of ... with the exception of lipid A from Vibrio cholerae, in which the presence of such an acid was not completely proved [36] Structural types of lipid A harboring a smaller number of fatty acids than...
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Báo cáo khoa học: Fatty acid composition of chylomicron remnant-like particles influences their uptake and induction of lipid accumulation in macrophages pot
Ngày tải lên :
16/03/2014, 12:20
... diameter of about 130 nm, which is within the range reported for human chylomicron remnants (50– 150 nm [27]), and that the lipid composition in terms of the percentage lipid mass of the various lipid ... rates of uptake of CRLPs of different fatty acid compositions by macrophages observed in the present study is due to differences in the oxidative state of the particles Evaluation of the lipid ... reach significance Interestingly, although the rate of uptake of the olive particles was comparable to that of palm CRLPs (Fig 3), the amount of lipid accumulated in the cells was significantly lower...
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Báo cáo khoa học: Theoretical study of lipid biosynthesis in wild-type Escherichia coli and in a protoplast-type L-form using elementary flux mode analysis potx
Ngày tải lên :
22/03/2014, 21:20
... FEBS Theoretical study of lipid biosynthesis in E coli 1025 Theoretical study of lipid biosynthesis in E coli D Kenanov et al Results Lipid biosynthesis in E coli The part of lipid metabolism studied ... EFMs are capable of producing all of the main metabolites that we are interested in Obviously, in order to produce one of the forms of lipid A or phospholipids, the production of fatty acids must ... Indeed, the mole number of ATP needed for one mole of lipid A in the core EFMs varies between 36 and 42 In contrast, the amount of NAD(P)H is 54 per mole of lipid A in all of these EFMs In this...
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Báo cáo khoa học: Complement factor 5a receptor chimeras reveal the importance of lipid-facing residues in transport competence doc
Ngày tải lên :
23/03/2014, 04:21
... Functional importance of lipid- facing residues in TMs of C5aR Fig Binding analysis of the wild-type and lipid- facing chimeras Competition binding analysis of the wild-type and lipid- facing chimeras ... M Klco et al Functional importance of lipid- facing residues in TMs of C5aR A Fig Molecular model of C5aR (A) Schematic representation of spatial arrangement of the TM helices in C5aR (shaded ribbons) ... be a product of receptor localization in the ER and not the result of an overall structural alteration Despite all of the chimeras being retained in the ER, all of the individual lipid- facing...
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Báo cáo khoa học: Proteomic characterization of lipid raft proteins in amyotrophic lateral sclerosis mouse spinal cord pot
Ngày tải lên :
23/03/2014, 04:21
... analysis to profile alterations of lipid raft-associated proteins in the spinal cord of the G93A SOD1 transgenic mouse model of ALS Alterations of a consortium of 67 lipid raft-associated proteins ... analyses of the lipid raft sample isolated from the same mouse; and (b) the protein was consistently identified in the independent Fig Evaluation of the isolation of lipid raft proteins Lipid raft ... PAGE and MS analysis of the lipid raft samples (A) SyproRuby staining of SDS ⁄ PAGE gel of the lipid raft samples from both WT and G93A transgenic mouse spinal cords (B) MS of all peptides eluted...
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Báo cáo khoa học: A transcription factor of lipid synthesis, sterol regulatory element-binding protein (SREBP)-1a causes G1 cell-cycle arrest after accumulation of cyclin-dependent kinase (cdk) inhibitors pdf
Ngày tải lên :
23/03/2014, 07:20
... activity in a wide range of genes involved in the synthesis of cholesterol, fatty acids, and phospholipids All mammalian cells require these lipids for the duplication of membranes in cell division ... of huge amounts of lipids in the transgenic hepatocytes may contribute to the inhibition of cell growth Effects of endogenous SREBP-1 on cell growth To determine the physiological relevance of ... considered to be inhibition of protein prenylation [35] Further studies of this strong lipid synthetic factor will reveal new aspects of a link between the regulation of lipid synthesis and the cell...
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Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc
Ngày tải lên :
23/03/2014, 09:20
... feature of this structure is its very high lipid content, consisting of up to 60% of the dry weight of the bacteria Among these lipids, the diesters of phthiocerol and phenolic glycolipids (PGLs) ... modification A Effect of the absence of DIM A on the virulence of M tuberculosis in mice B Fig MALDI-TOF mass spectra of the purified glycolipid from M tuberculosis PMM74:pPET1 (A) and of PGL-tb from ... biosynthesis of PGL-tb in M tuberculosis by catalyzing the reduction of the keto group of phenolphthiodiolone Construction of a DIM-less mutant of M tuberculosis H37Rv by disruption of the ppsE...
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Báo cáo khoa học: The equinatoxin N-terminus is transferred across planar lipid membranes and helps to stabilize the transmembrane pore pot
Ngày tải lên :
23/03/2014, 10:20
... amino acids of EqtII are exposed to the lumen of the pore; (b) the N-terminal part of EqtII extends to the trans side of the membrane in the final pore, i.e to the other side than the rest of the membrane-bound ... 5.5 (Fig 4), most of the histidines are protonated and thus the N-terminus of His6–EqtII carries a large excess of positive charge Consistently, the rates of closure and opening of the pore, as ... entrance of the pore, to enter the pore lumen and to clog it The rate of closures was voltage-dependent and increased with the magnitude of the negative applied voltage Furthermore, when the pH of...
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Báo cáo Y học: Structural determination of lipid A of the lipopolysaccharide from Pseudomonas reactans A pathogen of cultivated mushrooms doc
Ngày tải lên :
24/03/2014, 00:21
... region of the Fig Detailed view of the TOCSY spectrum of intact lipid A fraction from Ps reactans in which the correlations of the amide protons are plainly visible Ó FEBS 2002 Structure of lipid ... 3-O-deacylation of lipid a precursors in membranes of Salmonella typhimurium J Biol Chem 276, 9083–9092 22 Newmann, M.A., Daniels, M & Dow, M (1997) The activity of lipid A and core components of bacterial ... MALDI-TOF mass spectrum of intact lipid A fraction from Ps reactans Fig Negative-ion MALDI-TOF mass spectrum of ammonium treated lipid A fraction from Ps reactans A combination of homo- and hetero...
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relative permeability of petroleum reservoir
Ngày tải lên :
02/04/2014, 16:07
... parameters rock permeabilitywas the Kozeny-Carmen to measured the expresses permeabilityof a porousmaterialas a function of the productof the effective throughwhich path lengthof the flowing fluid ... Example of the use of the Corey equations.rl Preparea tabulation of k., vs So" for values of k,, ranging from 0.001 to 0.99 in stepwisefashion Determinevaluesof So"for eachexperimental valueof k., ... h\ !\ :.: tn lllt'a\uring -: '\ ruilflrof)' [r :: Leonard F Koederitz is a Professorof PetroleumEngineeringat the University of M fromtheU ni vers it yof issour iM.S , andP h.D degrees H M i...
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nanobiotechnology of biomimetic membranes
Ngày tải lên :
04/06/2014, 15:15
... chapters of this book look more closely at the technical detail of the production of lipid bilayer membranes, the characterisation of lipid bilayer membranes, and how lipid bilayer membranes ... of ion channels and lipid membranes 1.2 Interaction of Lipid Membranes with Transport Proteins An early study investigated the interaction of immunoglobulins with model lipid membranes produced ... develops the principles of membrane nanobiotechnology by discussing methods to produce lipid membranes, methods of characterising lipid membranes, and the application of membranes to produce biosensors...
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báo cáo hóa học: " Effects of collagen membranes enriched with in vitro-differentiated N1E-115 cells on rat sciatic nerve " doc
Ngày tải lên :
19/06/2014, 08:20
... Values of the ankle angular position () at initial contact (IC); opposite toe-off (OT); heel-rise (HR); toe-off (TO) of the stance phase Results are presented as mean and standard error of the ... Values of the ankle angular velocity (/sec) at initial contact (IC); opposite toe-off (OT); heel-rise (HR); toe-off (TO) of the stance phase Results are presented as mean and standard error of the ... al Journal of NeuroEngineering and Rehabilitation 2010, 7:7 http://www.jneuroengrehab.com/content/7/1/7 Page of 13 immediate cooptation with 7/0 monofilament nylon epineurial sutures of the transected...
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báo cáo hóa học: " β-Amyloid promotes accumulation of lipid peroxides by inhibiting CD36-mediated clearance of oxidized lipoproteins" pot
Ngày tải lên :
19/06/2014, 22:20
... evaluate the role of SRA/CD36-independent mechanisms, including those of additional scavenger receptor family members Because of the difficulty of culturing sufficient numbers of primary microglia ... No effect of Aβ was observed in these cells, demonstrating the specificity of Aβ inhibition of oxLDL binding to CD36 Discussion Numerous studies have demonstrated elevated markers of lipid peroxidation ... accumulation of Aβ in the brain and blood vessels in AD would foster the extracellular persistence of these pro-inflammatory lipids, thereby perpetuating lipid peroxidation Thus, Aβ binding of CD36...
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Báo cáo hóa học: " Mechanics of lipid bilayer junctions affecting the size of a connecting lipid nanotube" pptx
Ngày tải lên :
21/06/2014, 03:20
... the lipid tube is pulled to the inside of the vesicle and is directly fixed to the tip of the micropipette Also, there is a source of lipid attached to the mother vesicle wall The presence of lipid ... of these junctions by simple geometrical shapes and express the free elastic energy of the membrane in terms of the length of the LNT, its radius, the radius of the junction and the tension of ... is the radius of the nanotube of a given length L, Δi is the change in measured current with respect to the background, n is the number of moles of electrons transferred per mole of redox species...
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