pcr direct sequencing and sequence analysis
Báo cáo y học: "Enrichment of intersubtype HIV-1 recombinants in a dual infection system using HIV-1 strain-specific siRNAs" pps
... pCR XL TOPO vector Env-containing pCR XL TOPO clones were sequenced with EAD1, E80, and EAD2 (Davis sequencing) The env recombinant sequences and parental virus sequences were aligned using the ... by first PCR amplifying the env PCR product with nested primers (specific for v126-D and v120-A in the C2 region), cloning these products into pCR XL TOPO vector (Invitrogen), and then sequencing ... experiments and drafted the manuscript MA and KNN performed generation of v126-D/v120-A env recombinants through siRNA treatment, and subsequent screening and sequencing HB helped with analysis...
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The effects of clay a m e n d m e n t and composting on metal speciation in digested sludge liang qiao
... on sandstone oxyhydroxydes and clay (Duquet and V6dy, 1991), and similarly Zn in the residual fraction in this research was less than 5% (Fig 15) The composting process converted the organic and ... (Qiao and Ho, 1997), Cu and Zn hydroxides could not form Cu and Zn hydroxides theoretically form in pure solution at pH above 5.6 and 7.1, respectively The addition of red mud and the composting process ... fraction and finally Mn oxides in the sludge 100 *535 451 434 515 compost; the clay plasma and the sandstone oxyhydroxides sorb little Cu The composting process reduced the organic bound Cu and transferred...
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Tài liệu Finance and Economics Discussion Series Divisions of Research & Statistics and Monetary Affairs Federal Reserve Board, Washington, D.C.: Interest Rate Risk and Bank Equity Valuations doc
... example, DeYoung and Roland [2001] and Stiroh [2004] In what follows, we refer to both BHCs and commercial banks simply as “banks” and note the distinction between a holding company and an individual ... Interest Rate Derivatives As emphasized, for example, by Gorton and Rosen [1995], Choi and Elyasiani [1997] and Purnanandam [2007], banks can, and in many cases do, actively use derivatives to alter ... the failure of the standard CAPM to its static nature; see, for example, Hansen and Richard [1987], Jagannathan and Wang [1996], and Gomes et al [2003] More recently, Avramov and Chordia [2006]...
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... sharp maximum at 192 nm; and (b) a wide minimum between 208 and 220 nm The a-helix and b-strand contents of the protein were calculated with the online programs dichroweb [25] and k2d [26], respectively ... defined structure that presents two sharp minima at 288 and 295 nm, and two maxima at 274–283 nm (wide) and 290 nm (sharp) Storage stability and aggregation propensity The instability of the purified ... and cytochrome c (12.3 kDa) (Serva, Heidelberg, Germany) were used as molecular mass standards Absorption and CD spectroscopy A Jasco V-560 spectrophotometer (Jasco, Gross-Umstadt, Germany) and...
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... cDNA sequence and sequence alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the figure indicate the positions of the nucleotide sequence ... a consequence, individuals allergic to BGP require individual diagnosis and therapy, and an understanding of the structure of these allergens is essential to the improvement of diagnosis and the ... acid (B) Amino acid sequences of the peptides, the position in the complete sequence of Cyn d 24 is indicated the amino acids of number and number peptide sequences (Fig 2B), and an antisense primer,...
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Báo cáo Y học: A polymer with a backbone of 3-deoxy-D-glycero -D-galacto -non-2ulopyranosonic acid, a teichuronic acid, and a b-glucosylated ribitol teichoic acid in the cell wall of plant pathogenic Streptomyces sp. VKM Ac-2124 pdf
... Hz) and 1.78 (J3¢,4 12.4 Hz) The 1D NMR spectra could be interpreted from the analysis of 2D homonuclear 1H, 1H COSY, TOCSY and ROESY spectra and 2D heteronuclear 1H, 13C HSQC (Fig 1) and HMBC and ... [24] and based on the analysis of the products formed upon acid and enzymatic hydrolysis Acid hydrolysis afforded glucose and ribitol monophosphate, while a glycoside containing glucose and ribitol ... signals at d4.90 (J1,2 < Hz) and 5.07 (J1,2 3.6 Hz) and two signals of lower intensities at d4.55 (J1,2 7.9 Hz) and 4.66 (J1,2 7.9 Hz) (Table 2) Two signals at d1.93 and 2.07 were observed in the...
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Future R&D Environments A Report for the National Institute of Standards and Technology potx
... identifying future directions for science and technology and what trends they found in science and technology, in the economy, and in the organization and management of industrial research and development ... from biological, materials, and computer and information science and engineering) and sectors (industry, universities and other nonprofits, and government) The workshop and this report were organized ... range of possible directions that science and its technological applications may take, influenced by forces and trends in the economy and in industrial management and strength, and, of course,...
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Asthma WellnessKeeping Children with Asthma in School and Learning Liability & Litigation: A Legal Primer Asthma & Indoor Air Quality (IAQ)Asthma Management, Policies and Procedures.St r a i g h tBy Paul D. Houstont a l kIn School and Healthy: docx
... education and awareness programs for students and school staff Ensure that students with asthma receive education on basic management and emergency response and provide school staff and parents ... involved Sessions reviewed symptoms and early warning signs, use of medications and peak flow meter, and developed and implemented the school asthma action plan and emergency plan Participants were ... Initiative and the Harlem Lung Center to mobilize the health care community and provide educational information to families and caregivers The initiative included extensive data collection and analysis, ...
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bailin d., love a. cosmology in gauge field theory and string theory
... Nuno Antunes, Mar Bastero-Gil, Ed Copeland, Beatriz de Carlos, Mark Hindmarsh, George Kraniotis, Andrew Liddle, Andr´ Lukas and Paul Saffin for the particle and cosmological physics that we e have ... the Chandrasekhar limit and the supernova is born after the explosion The intrinsic luminosity of such supernovae is considered to be a constant That is, they are taken as standard candles and ... small and λ º e4 , then λ4 is negligible compared to e4 and B simplifies to 3e4 (2.65) B 64π With the mass-squared matrices of (2.40) and (2.41) for the (shifted) scalar and vector fields, and not...
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báo cáo hóa học: " Methyl salicylate 2-O-b-D-lactoside, a novel salicylic acid analogue, acts as an antiinflammatory agent on microglia and astrocytes" docx
... TZ and GD directed the work, contributed to design the study, reviewed the data and wrote the manuscript; XL performed cell culture, western blot analysis, ELISA assay and NO measurements; RL and ... pro-inflammatory cytokines (IL-6, IL-1b, and TNF-a) and the expression of inflammation-related proteins (iNOS, COX-1, and COX-2) in LPS-activated microglia and astrocytes, and we explored the association ... determine the direct inhibitory effects of DL0309 on COX-1 and COX-2 enzymatic activities, primary rat glial cells were pre-incubated with LPS (0.5 μg/ml) for 24 h Medium was then removed, and DL0309...
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Báo cáo hóa học: " Research Article Efficient and Precise Processing for Squinted Spotlight SAR Through a Modified Stolt Mapping" doc
... German Armed Forces for Radar and Laser Methods His research areas are design and analysis of highresolution SAR systems for air- and spaceborne reconnaissance, design and analysis of aperture synthesis ... variables and parameters in (1) are defined as follows: (i) t and τ are slow time and fast time, respectively; (ii) R is the slant range between a point scatterer and the antenna phase center and depends ... Stolt mapping within the defined bandwidths (squint angle = 20◦ ) wave number direction is noted For the given set of parameters and a squint angle of 20◦ , the needed bandwidth in range after the...
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Báo cáo khoa học: "Analysis of growth and light interception of balsam fir and white birch saplings following precommercial thinning D Pothier A" doc
... Wilson J, Hand DW, Sweeney DG (1984) Integrated analysis of growth and light interception in winter lettuce Analytical methods and environmental influences Ann Bot 54, 743-757 wood growth and leaf ... the field and the laboratory and T Hinckley and one anonymous reviewer for their Leverenz JW, Jarvis PG (1979) Photosynthesis in Sitka spruce VIII The effects of light flux density and direction ... season 1984) Growth analysis Growth analysis was performed using the relative growth rate (RGR) and its classical subdivision into specific leaf area (SLA), leaf weight ratio (LWR), and net assimilation...
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báo cáo khoa học: " The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway" pptx
... artichoke and cardoon total RNA was achieved using poly(dT)primer and M-MuLV RNaseH-RT (Finnzymes, Finland), following the manufacturer's instructions The PCR amplification of cDNA sequences ... http://bmr.cribi.unipd.it for sequencing To obtain the full length sequence, specific primers based on both, globe artichoke and cardoon, partial cDNA sequences, were designed for 3'- and 5'-end amplification ... HCT-For, HCT-Rev, HQT-For and HQT-Rev reported in Table 2) and PCR products were sequenced for SNP identification SNPs genotyping were carried out with the tetra-primers ARMS -PCR method [61,62] by...
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Báo cáo y học: "Mapping of IgE-binding regions on recombinant Cyn d 1, a major allergen from Bermuda Grass Pollen (BGP)" pps
... Biosystems, USA) using T7 and SP6 DNA sequencing primers The nucleotide sequence and deduced amino-acid sequences were compared by FASTA with those in the GeneBank database Sequence alignments were ... V Y K S K I Q F * 244 Figure Nucleotide sequence of Cyn d and its deduced amino acid sequence Nucleotide sequence of Cyn d and its deduced amino acid sequence The amino acid residues are numbered ... blot analysis and direct ELISA Dot blot analysis of Cyn d fragments and deletion mutants To evaluate the IgE-reactivity of purified whole rCyn d (F8), overlapping peptide fragments (F1-7) and...
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Báo cáo sinh học: " SNP mapping of QTL affecting growth and fatness on chicken GGA1" pptx
... Murani E., Schellander K., Schwerin M., Wimmers K., Identification of functional candidate genes for body composition by expression analyses and evidencing impact by association analysis and mapping, ... Based on the sequences provided by NCBI, proper PCR primers for amplifying each SNP were designed (Tab I) The 25 µL PCR reaction mixture contained 50 ng of chicken genomic DNA, X PCR buffer, 12.5 ... curves for 35 d BW and 42 d BW almost overlapped, and two QTL curves for 49 d BW and 70 d BW almost overlapped too (Fig 2) The additive effects of these QTL were both positive, and the dominant...
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Báo cáo y học: "Strict glycaemic control in patients hospitalised in a mixed medical and surgical intensive care unit: a randomised clinical trial"
... involved in the treatment and investigation were unaware of the randomised schedule and the block size Interventions Patients were randomly assigned to receive either standard insulin therapy or ... AR, AQ and LG participated in study conception, study design, data acquisition, data analysis and interpretation, and drafting of the manuscript, NS, MB, JT, JV, JV, CA, PA, EV, JCH, AY, WP and ... one, two and four hours if the patient had insulin infusion, and every four and six hours if no insulin was required according to the algorithm A protocol (see additional data files and 2), managed...
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Báo cáo y học: "Spinal Intramedullary Cysticercosis: A Case Report and Literature Review"
... bowel and bladder incontinence, and sexual dysfunction1,20 However, inflammatory reaction against the dead parasite is associated with perilesional edema, which can damage medullar parenchyma and ... intramedullary cysticercosis and whose clinical courses are stable The potential advantages of medical therapy alone include avoidance of surgery and treatment of surgically unreachable and multifocal cysticercus2,3,5,7,17 ... Brazil, Peru, Mexico, Korea and India19-20 Intramedullary cysticercosis often presents in the patients between 20 to 45 years old, with the youngest one years old and the oldest one 45 year’s...
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Báo cáo y học: "Mirror-Image Arachnoid Cysts in a Pair of Monozygotic Twins: A Case Report and Review of the Literature"
... a left middle cranial fossa cystic lesion with low and high signal intensities on T1- and T2-weighted images (d), (e) & (f) Brain CT and T 1and T2-weighted MRI of twin B shows a mirror-imaging ... in various structures such as handedness, hair whorl direction, tooth patterns, unilateral eye and ear defects, and even tumor locations and un-descended testicles However, only an extremely ... mirror-imaging of AC and discuss the possible clinical implications First, Helland and Wester11 reported a pair of 12-year-old MZ with mirror-imaging of AC in the cerebellopontine angle and indicated...
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Configuring a Stub Area and a Totally Stubby Area
... Baypointe, Vista, and Eastasman Capetown#show ip route Gateway of last resort is not set C O IA C O IA O IA O IA C O IA O IA 192.168.208.0/30 is subnetted, subnets 192.168.208.0 is directly connected, ... 192.168.208.0 is directly connected, Serial0/0 192.168.64.0/32 is subnetted, subnets O IA 192.168.64.1 [110/66] via 192.168.208.1, 00:07:32, Serial0/0 C 192.168.216.0/24 is directly connected, ... any means Step Capetown has several interarea (IA) routes and one external (E2) route In complex OSPF networks, a large number of external and interarea routes can needlessly weigh down some routers...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25