p63 basal stem cells were transformed into ar or ar cancer cells but rarely into chga neuroendocrine cancer cells
Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot
... treated IVD [18-24] An alternative cell source are adult stem cells, in particular, bone-marrow-derived mesenchymal stem cells (MSCs) The use of these cells would allow an autologous approach, reducing ... Grenoble, France) prior to isolation of mononuclear cells using a Histopaque 1077 gradient (Sigma, Poole, UK) Cells were cultured for days and any nonadherent cells were removed MSCs (characterised by ... replacements Informed consent from the patients and local ethical committee approval were obtained Bone marrow was negatively sorted for haematopoietic cells using RosetteSep (STEMCELL Technologies SARL,...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... data suggest the paracrine effects of bone marrow cells on supporting endogenous cells to regenerate However, the potential of bone marrow stem cells or MSCs to differentiate into IPCs has been ... MSCs into IPCs (A) MSCs were treated without (DMSO) or with 50 μM of PD98059 or LY294002 and MTT assay were performed at 48 hours (B) MSCs were pretreated without (DMSO) or with PD98059 or LY294002 ... size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells induced...
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Báo cáo y học: "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions" pdf
... myocyte specific markers Immunocytochemistry The medium was first removed and the cells were washed twice with PBS, fixed for 30 with 4% paraformaldehyde Cells were permeabilized for 20 with 0.1% ... (UHQ) system The silicon wafers were purchased from Compart Technology Ltd (UK) and were cut into × cm2 cuts before use They were cleaned by 5% (v/v) Decon 90 solution (Decon Laboratories), followed ... blocked for 30 in 5% normal goat serum Cells were then incubated with the primary antibody (Ab) (either mouse anti-a-actinin (sarcomeric) at a dilution of 1:200, or mouse anti-myosin cardiac heavy...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... Moreover, mapping enhancer repertoires provides an enormous resource for the development of reporters for isolation and characterization of rare human cell populations, such as the progenitor ... disease into a much smaller regulatory space that can be more effectively examined for recurrent variants that are associated with disease (Figure 2a) The function of these regulatory variants ... marks cover only 4% of the hESC genome, but well over 10% of the human fibroblast genome Parallel observations have been made independently in mice, where large H3K9me2-marked regions are more frequent...
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báo cáo khoa học: " Transcriptomic analysis of pluripotent stem cells: insights into health and disease" potx
... differences were proposed to be a consequence of comparing cells of different genetic origins [60], laboratory-to-laboratory variation [61], and the iPSC passage number [62] Later, hiPSCs were described ... embryonic stem cell (ESC) transcription factors Oct4, Sox2 and Nanog form an autoregulatory network by binding their own promoters as well as promoters of the other core members These three core factors ... hiPSCs and hESCs were largely similar, and that the observed hiPSC differences were similar to normally occurring variation among hESCs Additionally, Bock et al established a scoring algorithm to predict...
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Báo cáo y học: " Influence of hypoxia on the domiciliation of Mesenchymal Stem Cells after infusion into rats: possibilities of targeting pulmonary artery remodeling via cells therapies " doc
... (n = 26) Mesenchymal Stem cells (MSCs) are bone marrow nonhaematopoietic stem cells that are multipotent and can differentiate into bone, cartilage and connective tissue cells [2-4] They also ... vascular wall following graft vasculopathy or arteriosclerosis [1] When integrated to the vascular wall, HSCs differentiate into mature vascular cells with an endothelial or smooth muscle cells ... The participation of stem cells in several vascular diseases pathogenesis was first proved with haematopoietic stem cells (HSCs) In this regard, following bone marrow engraftment, HSCs were observed...
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Generation of porcine induced pluripotent stem cells and their differentiation into cardiac lineages
... pluripotent cells are studied and characterized The pluripotent stem cells exhibited morphology and genomic markers of pluripotency These cells were also finally differentiated into cardiomyocytes ... (Odorico, Kaufman, & Thomson, 2001) The cardiomyocytes must be pure, and no parental stem cells should be used as stem cells are oncogenic and will result in the formation of tumours Various cardiomyocytes ... mechanically into smaller pieces, and they were transferred onto fresh feeders that were prepared earlier 4.10 Characterization of Porcine Induced Pluripotent Stem Cells Several experiments were performed...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... of the earliest markers of a haematopoietic cell/ progenitor Another haematopoietic progenitor marker is c-Kit which is the receptor for the cytokine Stem Cell Factor (SCF/ Steel Factor) This ... progenitors In contrast HS-27A does not secrete cytokines but has been shown to support formation of typically haematopoietic “cobblestone areas” from progenitor cells of human bone marrow origin (Torok-Storb ... pluripotency markers Nuclear staining for transcription factor OCT4 (D) and cell surface staining for Tra 1-60 (E) show that more than 90% of the cells in all colonies stain positive for these two markers...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... Int J Med Sci 2011, searchers have transplanted the bone marrow stem cells into the necrotic femoral heads, and results show bone marrow stem cells can remove vascular lesions and promote angiogenesis ... shape of femoral head was integrity and the articular cartilage largely preserved The articular cartilage was glossy and smooth, a fraction of which present dark red (Figure 5C) Distribution of ... of femoral head necrosis was established according to previously reported [8] Weight loading area of femoral heads was exposed and treated with liquid nitrogen for 3-5 until the articular cartilage...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... at 37oC, (B1) 4oC for 24h, (C1) 25oC for 24h, (D1) 4oC for 48h, (E1) 25oC for 48h The corresponding karyotypes (A2 to E2) were analyzed by the ISIS imaging software (Metasystems GmbH, Altussheim, ... the sides with parafilm (for minimal evaporation), and placed in either a 4oC refrigerator, or back into the incubator reset at 25oC An atmosphere of 5% CO2 was maintained only for the physiological ... implications for the salvage of hESC culture during infrequent occurrences of incubator break-down and power failure, either in small-scale culture for research or large-scale culture within bioreactors...
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
... authors and readers, this journal provides supporting information supplied by the authors Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or ... and characterized as previously described [38] All experiments were performed on the second or third passage of cardiac fibroblasts after starvation in serum-free DMEM for 24 h The cells were then ... [3H]-proline incorporation under normoxic or hypoxic culture conditions To further clarify the molecular mass range of important active factors in the MSCs-CM, the medium was divided into B Fig MSCs-CM...
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Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx
... outcome in described stem cell types Stem cell type BMP signal outcome Embryonic stem cells (i) Embryonic stem cells (ii) Hair-cell progenitors (inner ear) Mesodermal progenitor cells Stemness upkeep ... signaling are dispensable for stemness, even though they are capable of supporting it under specific conditions as described before BMP as an initiator of the germ line Cells of the germ line are unique ... epithelial duct cells, ID2 expression leads to down-regulation of the NeuroD target gene PAX6, an important factor for final differentiation of the progenitors into endocrine cells In parallel, BMP4...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the Rex-1 and b-actin genes but not the embryonic ... methylases, which could establish a memory mark for subsequent cell divisions [38] This would be similar to memory elements in drosophila, which are important for developmental stage-specific gene ... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells...
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NEURAL STEM CELLS NEW PERSPECTIVES doc
... horizontal cells display stem cell characteristics but are clearly distinct from the previously described Type-1 and Type-2 cells Horizontal Type-1 neural stem cells like radial Type-1 stem cells ... neuroblasts (Acells) and the ependymal lining B1 -cells are quiescent and, based on thymidine incorporation assays and electronmicroscopic analysis, they rarely divide B2 -cells are more displaced towards ... radial and horizontal stem cells is not clear, horizontal cells rarely generate radial Type-1 cells in viral lineage tracing experiments Interestingly, activated neurogenic stem cells in the...
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Stem cells 2013
... Shinya Yamanaka Stem Cells Make Copies of Themselves Through Cell Division, or … Stem Cells Adapt for Many Parts of the Body Stem Cells Brain Bone marrow Fat Heart Pancreas Pluripotent Stem Cell Sources ... human embr y onic stem cells differentiated into brain cells and inser t ed into mouse brain make the mouse think like a human? • Outcome If human stem cells were inser t ed into developing non-human ... Legality of embr y onic stem cell research • Legality of research using somatic cell nuclear transfer • Authority of states to expand, fund, limit or prohibit stem cell research • Anything to with...
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Stem CELLS and the FUTURE OF REGENERATIVE MEDICINE pdf
... undifferentiated stem cells for research and therapy To obtain undifferentiated stem cells for research and therapy To obtain undifferentiated stem cells that are genetically matched to recipient for research ... Contents EXECUTIVE SUMMARY 1 PROJECT OVERVIEW AND DEFINITIONS ADULT STEM CELLS 19 EMBRYONIC STEM CELLS 31 OPPORTUNITIES FOR AND BARRIERS TO PROGRESS IN STEM CELL RESEARCH FOR REGENERATIVE MEDICINE ... adult stem cells are multipotent For example, it has been reported that stem cells from the bone marrow, a mesodermal tissue, can give rise to the three major types of brain cells, which are ectodermal...
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Atlas of Human Pluripotent Stem Cells doc
... reviews or scholarly analysis Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter ... However, both lines were reported to harbor karyotype abnormalities It has yet to be determined whether the embryos were originally defected or whether the abnormalities detected are due to the method ... pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for studying the fundamentals of early human development and organogenesis, for cellbased...
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Stem Cells in Human Reproduction Basic Science and Therapeutic Potential Second Edition pot
... the publishers nor the authors can be held responsible for errors or for any consequences arising from the use of information contained herein For detailed prescribing information or instructions ... family members or friends to donate oocytes, but “anonymous” oocytes are scarce unless considerable amounts are offered to potential donors Donors are reluctant to come forward for two reasons: ... from early stage primordial germ cells (PGCs) and primordial follicles In these protocols, PGCs and primordial follicles are exposed to a variety of growth factors, are cocultured with ovarian cells...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
... of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells derived ... ES cells and EG cells, the ED of TS cells to ES cells (77) is greater than that of EG cells to ES cells (49), confirming the similarity of EG cells to ES cells DNA methylation profiles that are ... even more complex DNA methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells...
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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... Technologies), which performs background subtractions Features that were flagged according to the software algorithm or that were below background value were excluded from further analysis Normalization Transcriptome ... Acknowledgements This work was supported in part by a grant-in-aid from the Third-Term Comprehensive 10-Year Strategy for Cancer Control, health science research grants for Research on the Human Genome ... maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood Stem...
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