p1 na p1 interaction study by co immunoprecipitation
Báo cáo khoa học: "Identification and characterisation of a novel anti-viral peptide against avian influenza virus H9N" pps
Ngày tải lên :
12/08/2014, 04:21
... result regarding the NA -P1 HAt -P1, NA -P1 interaction study by Coimmunoprecipitation In order to verify the binding ability of the peptide P1 with HAt and NA proteins through Co- IP method, these ... protein-protein interactions Construction of recombinant pC-HAt, pC -NA and pC -P1 and in vitro transcription and translation The HAt and NA gene of AIV strain H9N2 as well as the recombinant peptide gene P1 ... the NA, HAt and P1genes Primers Sequence pY -NA- Fa pY -NA- R pY-HAt-F pY-HAt-R pH -P1- F pH -P1- R pHybLex/Zeo-F pHybLex/Zeo-R pYESTrp2-F pYESTrp2-R pC-HA-F pC-HA-R pC -NA- F pC -NA- R pC -P1- FP pC -P1- RP...
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in vitro transcription and translation protocols
Ngày tải lên :
11/04/2014, 09:47
... yield of RNA product can be caused by several conditions, including precipitation of DNA by spermidine in the transcription buffer, RNase contamination, carryover of residual contaminants or salts ... the final concentration (final cont.) of radionucleotides commonly used in RNA probe synthesis, in a 20-pL reaction volume Thiol-substituted rNTPs are incorporated less efficiently by the RNA polymerases ... Translation Protocols Edited by: M J Tymms CopyrIght 1995 Humana Press Inc., Totowa, NJ 13 14 Schweinfest cDNA Subtraction et al Hybridization Normal Tumor mRNAu mRNAr ds cDNAu with 5’ EcoRl end 3’...
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Cách gieo hạt lan nẩy mầm trong điều kiện in-vitro ppt
Ngày tải lên :
04/07/2014, 23:20
... giống lan khác biết: Rhizoctonia repéns đặc biệt cho Cattleya, Laelie, Cypripedium Rhizoctonia mucoroides cho Vanda Phalaenopsis Rhizoctonia lanuginosa cho Oncidium, Miltonia Nấm cung cấp đường ... gieo hạt lan môi trường dinh dưỡng nhân tạo có khoáng, đường hạt lan nảy mầm với tỷ lệ cao Hiện nay, phương pháp nuôi cấy mô phương pháp nhân giống lan qui mô công nghiệp Môi trường nuôi cấy: ... điều kiện invitro Môi trường cấy chuyền môi trường gieo hạt bổ sung thêm 10% nước dừa cho protocorm phát triển nhanh Khi chồi lớn chuyển sang môi trường môi trường bổ sung thêm 50g dịch chiết...
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báo cáo khoa học: " LysoPC acyltransferase/PC transacylase activities in plant plasma membrane and plasma membrane-associated endoplasmic reticulum" ppt
Ngày tải lên :
12/08/2014, 05:20
... Incorporation of [1-14C]18:1-CoA into native PC and PE was analysed in the membrane fractions obtained from the 10-step counter current two-phase separation The incorporation of [1-14C]18:1-CoA ... [1-14C]18:1-CoA (Fig 4A) or [1-14C]16:0-CoA (results not shown), the radiolabel was recovered predominantly as free fatty acids, indicating that the plasma membranes contained a highly active acyl-CoA ... acyl-CoA pool in its vicinity The remaining acyl-CoA radiolabel was recovered in phospholipids, predominantly PC (Fig 4B) The labelling was higher with [1-14C]18:1-CoA than with [1-14C]16:0CoA...
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Báo cáo y học: "Comparative study of serum Na+ and K+ levels in senile cataract patients and normal individuals"
Ngày tải lên :
03/11/2012, 09:49
... the study Case and control groups were both examined by an expert ophthalmologist and routine ophthalmic examinations including visual acuity determination, slit lamp examination, ophthalmoloscopy ... serum + Na of case and control ones Table Comparison of Mean & SD of serum Na+ , K+ in 155 patients with cataract and 155 without cataract Study group Serum Age-related cataract patients Control ... flam photometry method by the aid of SEAC model FLP20, Italy Outcoming data was assessed by spss 11 software and mean of aboveelectrolytes in both groups were compared by t-test Results Int J...
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Tài liệu Red Hat Linux Networking and System Administration P1 docx
Ngày tải lên :
12/12/2013, 22:15
... France; by International Thomson Publishing for Germany, Austria, and Switzerland; by Distribuidora Cuspide for Argentina; by LR International for Brazil; by Galileo Libros for Chile; by Ediciones ... Peru; by WS Computer Publishing Corporation, Inc., for the Philippines; by Contemporanea de Ediciones for Venezuela; by Express Computer Distributors for the Caribbean and West Indies; by Micronesia ... enter a domain name and have it converted into an IP number that is the actual address of a computer The name-to-number conversion is done by the Domain Name System (DNS), which is covered in Chapter...
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Báo cáo khoa học: Selectivity of pyruvate kinase for Na+ and K+ in water/dimethylsulfoxide mixtures docx
Ngày tải lên :
08/03/2014, 02:20
... site in the Na+ -pyruvate kinase complex than in the K+-pyruvate kinase complex We carried out additional kinetic studies in order to further characterize the competition between Na+ and Mg2+ ... of pyruvate kinase The effect of Na+ and K+ concentration on the activity of pyruvate kinase was determined in mixtures with various dimethylsulfoxide concentrations; a saturating concentration ... partition of Na+ and K+ into the divalent metal cation binding site, the Ki app for Na+ and K+ at different cosolvent concentrations were compared At variance to the difference in K0.5 app for Na+ and...
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Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
Ngày tải lên :
26/10/2012, 08:57
... mechanism (7) However, the use of conventional vesicles cannot fully overcome their binding with serum components and uptake by mononuclear phagocyte system (MPS) To overcome such problems, long circulating ... another h, followed by ultrasonication in an ice bath for 15 Then the sample of LCL was delivered to the Zhejiang California International NanoSystems Institute, and was processed by a microfluidizer ... and conjunctive RGD-LCL equivalent to 15μg/mL RGD were analyzed by HPLC to ascertain the status of RGD A Hypersil-BDS-C18-column (4.0 × 250 mm, Thermo, USA) was used with a mobile phase consisting...
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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
Ngày tải lên :
31/10/2012, 15:28
... when high concentrations are applied [25] Total RNA extraction and microarray hybridization Total RNA was extracted using RNase kit (Qiagen, Hilden, Germany) and its quality was confirmed by electropherograms ... labeled cRNA using the Affymetrix Test Array, cRNA was converted to cDNA using GeneChip One-Cycle cDNA Synthesis Kit (Affymetrix) Fifteen micrograms of 29 labeled and fragmented cRNA were subsequently ... G-Protein-MAPK, NGF, and PDGF signaling This finding corroborates the interaction observed between various intracellular signaling pathways and apoptosis induced by 1mM concentration of ethanol in...
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Analysis, sequencing and in vitro expression of PCR products
Ngày tải lên :
25/10/2013, 22:20
... DNA amplification products Smearing of DNA amplification products is most often associated with nonspecific primer annealing conditions, poor quality DNA or low copy number template, or a combination ... archaeology (Chapter 3) DNA sequencing chemistry and automation Dideoxy terminator DNA sequencing (5) involves the incorporation of 2′,3′dideoxynucleotide ‘terminators’ into nascent DNA chains (5; Figure ... ddT termination products T DNA sequencing gel Figure 5.3 Dideoxynucleotide chain termination approach for DNA sequencing An oligonucleotide primer is extended by DNA polymerase that incorporates...
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Tài liệu Red Hat Linux Networking and System Administration P2 pptx
Ngày tải lên :
12/12/2013, 22:15
... network are likely to become corrupted, whether by hardware or power failure, by misconfiguration or user error (to the extent that the latter can be avoided), or by malicious or inadvertent intrusion ... above mere “computer user” status The Linux system administrator does not achieve that mantle by having purchased a computer but instead by having taken full control of what his or her computer ... can configure Red Hat Linux to prompt users to so And what to about old accounts? Perhaps someone has left the company What happens to his or her account? You probably don’t want him or her to continue...
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Tài liệu 10 Red Hat® Linux™ Tips and Tricks pptx
Ngày tải lên :
21/12/2013, 04:18
... this scenario, you want to share /dev/sde1 with /dev/md0 if it should need it To that, you must configure the /etc/mdadm.conf file and define a spare-group name In /etc/mdadm.conf, start off by listing ... your competitive edge Check out our complete Red Hat Linux curriculum at www.globalknowledge.com/redhat For more information or to register, visit www.globalknowledge.com or call 1-800-COURSES ... /dev/sdd1 /dev/sde1" >> /etc/mdadm.conf Scan the RAID arrays for the current details, and add it to the file: mdadm -D -s >> /etc/mdadm.conf /etc/mdadm.conf should now contain something like the following:...
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Tài liệu 7 More Red Hat® Linux™ Tips and Tricks docx
Ngày tải lên :
21/12/2013, 04:18
... records out 143654912 bytes (144 MB) copied, 5.66717 seconds, 25.3 MB/s 249+0 records in 249+0 records out 261095424 bytes (261 MB) copied, 11.5736 seconds, 22.6 MB/s 388+0 records in 387+0 records ... like this: Copyright ©2007 Global Knowledge Training LLC All rights reserved Page $ dd if=/dev/zero of=/tmp/demo bs=1M count=1536 33+0 records in 33+0 records out 34603008 bytes (35 MB) copied, ... server? by Chris Tatman Release Found: Red Hat® Enterprise Linux® and Red Hat® Enterprise Linux® Resolution: The logging of DNS queries can be enabled by running the rndc command To so run the command...
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Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc
Ngày tải lên :
18/02/2014, 04:20
... administration of native ETA, and evaluated for their content of internalized toxin by nonreducing (upper blots) and reducing SDS ⁄ PAGE (lower blots) followed by western blot analysis with the polyclonal ... ETA, and evaluated for their content of LRP1 (upper blot) or EEA1 (lower blot) by reducing SDS ⁄ PAGE followed by western blot analysis Each lane contained 30 lg (a-LRP1 blot) or 50 lg (a-EEA1 blot) ... weight), and evaluated for their content of internalized toxin by reducing SDS ⁄ PAGE followed by western blot analysis with the polyclonal antibody against ETA Each lane contained 50 lg of endosomal...
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Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
Ngày tải lên :
18/02/2014, 08:20
... were scored as follows: a pattern with the anterior-most additional digit being a digit scored 100%; a wing with an additional digit anteriorly scored 66%; a wing with an additional digit scored ... points by washing with NaCl ⁄ Pi and lysis of the cells using mL of Trizol (Invitrogen) per well Total RNA from the cells was extracted according to the manufacturer’s protocol In order to eliminate ... S-4o-9c-dh-RA Fig Transcriptional activation of synthetic and natural RARE by S-4o9cDH-RA HC11, HeLa and P19 cells (A–D) were transfected with a luciferase reporter plasmid regulated by a minimal RARE in...
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Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx
Ngày tải lên :
19/02/2014, 16:20
... protocol supplemented with on-column RNasefree DNase treatment to remove baculoviral DNA as described by the manufacturer Completeness of removal of baculoviral DNA was monitored by including control ... wild-type virus or recombinant virus containing human iPLA2c cDNA After 48 h, cells were pelleted by centrifugation, resuspended in ice-cold NaCl/Pi and repelleted The supernatant was decanted ... presence of a naturally occurring NcoI site within the 83 construct, an AflIII restriction site was utilized at the 3¢-end of this construct (instead of NcoI) to generate a compatible cohesive end...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
Ngày tải lên :
20/02/2014, 01:20
... prediction by computer analysis revealed that the secondary structure of the 3¢-end of the 122 nt RNA fragment was unmodified compared to wild-type RNA This discrepancy can be explained by differences ... initiation site of RNA synthesis by recombinant HCV NS5B from different viral strains Analysis of RNA synthesized in one round of synthesis RNA products shown in Fig were obtained under conditions where ... manuscript This work was supported by the Agence Nationale de Recherche contre le Sida (ANRS), the Centre National de la Recherche Scientifique (CNRS), the Institut National ´ ´ de la Sante et de la...
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Tài liệu Báo cáo khoa học: "Structural and Topical Dimensions in Multi-Task Patent Translation" ppt
Ngày tải lên :
22/02/2014, 03:20
... discriminative approach to machine translation In Proceedings of the joint conference of the International Committee on Computational Linguistics and the Association for Computational Linguistics (COLING-ACL’06), ... symmetrical and asymmetrical parallel corpora In Proceedings of the 23rd International Conference on Computational Linguistics (COLING’10), Beijing, China Alexandru Ceausu, John Tinsley, Jian ... Schroeder (2007) Model combination is accomplished by adding additional language model and translation model features to the log-linear model and tuning the additional meta-parameters by standard minimum...
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