... Helkala EL, Laakso MP, Hanninen T, Hallikainen M,Alhainen K, Soininen H, Tuomilehto J, Nissinen A: Midlife vascularrisk factors and Alzheimer&apos ;s disease in later life: longitudinal,population ... purposes)Journal of NeuroinflammationOpen AccessReviewThe microglial NADPH oxidase complex as a source of oxidative stress in Alzheimer&apos ;s diseaseBrandy L Wilkinson* and Gary E LandrethAddress: Alzheimer ... peroxidation and oxidative imbalance:early functional events in Alzheimer&apos ;s disease. J Alzheimers Dis2004, 6:171-175.26. Markesbery WR: Oxidativestress hypothesis in Alzheimer&apos ;s disease....
... GGCTCTGGAACTGAATAAAGCGACpes1 A2 reverse GTCCCATATATCCGCTTGCAATCTpes1 A4 forward TCTGACTCCGTCGATAGCTAGCATpes1 A4 reverse CCAGATCCTCACGACTGATAAGCTCpes1C2forward GAGATCTAGATACCCATGCAGCCCTGTCpes1C2reverse ... GATATCGAATTCGCCTCAAACAATGNested forward GAGACCTAGGAAGCAATGTCTCCGCAACATTTGGCGACATGGTCTCATATNested reverse GAGACCGCGGAAGCTTCTGGACCTTTTCGCGTGTTGCTTCCGACATAGGANRP synthetase in Aspergillus fumigatus ... pes1 in pro-tecting A. fumigatus against oxidative stress. Semiquantitative analysis of pes1 expression hasconfirmed that the gene is present, and differentiallyexpressed, in four strains of A. ...
... G, Aliev G, Irai K, Takeda A, Balraj EK, Jones PK,Ghanbari H, Wataya T, Shimohana S, Chiba S, Atwood CS, PetersenRB, Smith MA: Oxidativestress is the earliest event in Alzhe-imer&apos ;s disease. ... After 15 min, the reaction was stopped andabsorbance immediately read at 450 nm. Oxidized pro-tein standards, internal controls and blanks were alwaysassayed at the same time and in the same ... therelationship between brain inflammation responses and oxidative stress has not yet been clearly delineated in AD.For example, its is possible to consider these two events aselements of the same...
... inflammation after anoxia. The Figure 2 shows activities of AS, AL and arginase in the study. AS and AL activities increased in all the three brain regions significantly in anoxia suggesting ... stressin anoxia and reperfusion and suggests a possible role for anti oxidants in preventing neuro-degeneration in anoxia and reperfusion injuries to brain. The increased arginase and sustained ... (20). Apart from NO synthesis L-arginine may also serve as a substrate for glutamate formation and may also provide increased substrate for arginase (50). No significant changes in the activity...
... dismutase and catalase activities in the hippocampus of adult rats afterpilocarpine-induced SETable 1 shows superoxide dismutase and catalase activ-ities in the hippocampus after seizures and SE ... Oxidativestressin the hippocampus after pilocarpine-induced status epilepticus in Wistar ratsRivelilson M. Freitas, Silvaˆnia M. M. Vasconcelos, Francisca C. F. Souza, Glauce S. B. Vianaand ... data suggest that the hippocampus doesnot use superoxide dismutase as the major free-radical-scavenging system [9,30]. It probably uses other scav-enging systems (catalase and GSH).Pilocarpine-induced...
... receptor-based mechanisms are modulatedby [Ca2+]e: (a) the Ca2+-sensing receptor is activated bymillimolar changes in [Ca2+]e, and is widely distributed in mammalian tissues including brain ... mechanisms may act alone or in concert with nonselective Ca2+channels in producingsignificant excitotoxic Ca2+increases following ischemicinsults.TRP channels as candidates for paradoxicalCa2+-increasesTRP ... latter study the authors also demonstratedthat the increase in superoxide radical formation ispredominantly associated with extramitochondrialphospholipase A( 2) (PLA2) activation, and it does...
... and outlined their relevance as significant con-trolling steps of energy metabolism in parasites subjected to oxidative stress. AbbreviationsAcCoAS, acetyl-coenzyme A synthetase; Cat, catalase; ... fluxes in live parasites.ResultsKinetic characterization of EhPFOR in amebalextractsPFORs in several anaerobic parasites have been foundattached to plasma and hydrogenosomal membranes[20,21], ... acetyl-CoA. Basal activity withNADH and the extract was always subtracted. Completeinhibition of the ADH activity with pyrazole was deter-mined separately in the ADH assay. Acetyl-CoA synthetaseactivity...
... genesregulating peroxisomal and microsomal oxidation pathways was analyzedby RT-PCR. In addition, glutathione peroxidase and catalase activities, aswell as thiobarbituric acid reactive substances, ... mirrorsthe effects observed for GPX and catalase activities,thus indicating that a diet based on MCTs raises therisk of ROS production. The TBARS concentrationwas strongly increased after fasting ... skeletalmyopathy also occur in long-chain fatty acid oxidationdefects [4]. During these catabolic situations, long-chain fatty acids cannot be oxidized, and accumulate in tissues as long-chain acyl-CoAs and...
... Furusawa S, Kimura E, Kisara S, Nakano S, Murata R, Tanaka Y, Sakaguchi S, Takayanagi M, Takayanagi Y, Sasaki K: Mechanism of resistance to oxidative stressin doxorubicin resistant cells. Biol ... kinase phosphatases [3]. MAP kinase phosphatases(MKPs) are a group of dual-specificity phosphatases thatinactivate MAPKs by dephosphorylating their threonineand tyrosine residues. At least ... pro-inflammatorycytokine signaling cascade, and the delayed and sustainedactivation is mediated by ROS [45], which inactivateMAP kinase phosphatases by reacting with catalyticcysteine and causing...
... Western blotting detection system (Amersham).Statistical analysisData were expressed as mean±SD. For comparison ofmeans, Student s t-test was performed using SPSS 9.0(SPSS Inc., USA) statistical ... 5'-TTACTTTCTTGTTCAGCGACCGA-3' and antisense 5'-C ACCTTCGTATAGAATGTCCGCA-3', Cu/Zn-SOD (541 bp): sense 5'-AGGATTAACTGAAGGCGAGCATG-3' and antisense 5'-GCCCAAGTCATCTTGTTTCTCGT-3', MTH1 ... (www.ncbi.nlm.nih.org) sequences. Mn-SOD(782 bp): sense 5'-GATGTGTGGAGCACGCTTACT-3' andantisense 5'-CACAATGTCACTCCTCTCCGAATTA-3',Catalase (763 bp): sense 5'-TTACTTTCTTGTTCAGCGACCGA-3'...
... increasing O3(Figs. 1 396 C. Gaucher et al.season suggested that less precursors and less energy was allo-cated to repair of injured foliar tissues. As PEPC transformedPEP to OAA and as ... symptomatic if at least2% of his area was injured.2.3. Enzymatic analysis2.3.1. In vivo nitrate reductase assayAt the field site, in parallel with the harvests and at all sam-pling dates, NR (E.C. ... photorespiratory pathway) in Pinus taeda needles exposed to air pollution.As a shade tolerant, slow growing species [3], sugar maplehas a low assimilation rate, leading to a compromise be-tween maximizing...
... pathway is intrinsically defective in SSc.Keywords: nifedipine, oxidative stress, sVCAM-1, systemic sclerosis, VEGFIntroductionSystemic sclerosis (SSc) is a connective tissue diseasecharacterised ... endothelium-related indices, including increased plasma levels of mark-ers such as soluble vascular cell adhesion molecule 1(sVCAM-1). Thus, sVCAM-1 could be a useful parameterfor vascular assessment [6] and ... expression of adhe-Table 2Serum concentrations of vascular markers in patients with systemic sclerosis (SSc) at baseline and in controlsSerum constituent Controls (n = 20) SSc patients at baseline...
... hypothesis that oxida-tive agents play a role in situ in chondrocytes and in cartilage changes in OA. These results also support theconcept that antioxidative agents may prevent oxidative stress- induced ... group, which was macroscop-ically normal). In these donor matched pairs of articular cartilage samples,antioxidative potential of the tissue was measured using anassay that is based on reduction ... closely related to the increase in senes-cence-associated β-galactosidase expression in humanchondrocytes, suggesting that chondrocyte senescence,at least in part, participates in the age-related...