oxidative stress in alzheimers disease brain new insights from redox proteomics
Báo cáo khoa học: Structure–cytotoxicity relationships in bovine seminal ribonuclease: new insights from heat and chemical denaturation studies on variants ppt
Ngày tải lên :
22/03/2014, 17:20
... O,
Tancredi T & Picone D (2003) Role of the hinge
peptide and the intersubunit interface in the swapping
of N-termini in dimeric bovine seminal RNase. Eur J
Biochem 270, 4729–4735.
19 Leich ... relationships in bovine seminal
ribonuclease: new insights from heat and chemical
denaturation studies on variants
Concetta Giancola
1
, Carmine Ercole
1
, Iolanda Fotticchia
1
, Roberta Spadaccini
2
, ... bovine seminal ribonuclease in which the chains swap their
N-termini; M=M, unswapped dimer of bovine seminal ribonuclease; NCD, noncovalent dimer; PDB, Protein Data Bank; RI, ribonuclease
inhibitor;...
- 12
- 300
- 0
Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"
Ngày tải lên :
26/10/2012, 09:07
... in brain in anoxia as observed by in-
crease in NOx concentration in this study support the
earlier findings of NO involvement in pathophysiol-
ogy of hypobaric hypoxia in brain (42-43). In ... synthase. The increased
activities of argininosuccinate synthetase and argininosuccinate lyase suggest the increased
and effective recycling of citrulline to arginine in anoxia, making nitric ... arginine at the site of synthesis.
Arginine is a semi essential amino acid and in CNS, its
availability depends upon: (a) uptake from the circu-
lating arginine and (b) recycling of citrulline...
- 8
- 622
- 0
báo cáo hóa học: " Brain inflammation and oxidative stress in a transgenic mouse model of Alzheimer-like brain amyloidosis" pptx
Ngày tải lên :
19/06/2014, 22:20
... AD-like brain Aβ amyloidosis model.
Previously, we have shown that a full dose of
indomethacin alone despite a significant reduction in
brain inflammation had only a marginal effect on brain
oxidative ... purposes)
Journal of Neuroinflammation
Open Access
Research
Brain inflammation and oxidative stress in a transgenic mouse
model of Alzheimer-like brain amyloidosis
Yuemang Yao
1
, Cinzia Chinnici
1
, Hanguan ... Trojanowski JQ: Increase of
brain oxidative stress in mild cognitive impairment: a possi-
ble predictor of Alzheimer's disease. Arch Neurol 2002,
59:972-976.
7. Mecocci P: Oxidative stress in mild...
- 9
- 490
- 0
báo cáo hóa học: " The microglial NADPH oxidase complex as a source of oxidative stress in Alzheimer''''s disease" ppt
Ngày tải lên :
19/06/2014, 22:20
... important role in the patho-
genesis of oxidative damage in the AD brain.
Oxidative damage directly stemming from peroxynitrite
formation has been examined in the AD brain. Immuno-
cytochemical 4-hydroxynonenal ... global inhibition
of src-family tyrosine kinases or inhibition of phosphati-
dylinositol-3 kinase (PI3K) attenuates ROS production.
These findings suggest that these kinases are involved in
upstream ... advances in proteomics have also identified
specific proteins which are nitrated in the AD brain
including β-actin, enolase, and L-lactate dehydrogenase
[94].
The role microglia play in peroxynitrite-mediated...
- 12
- 413
- 0
Tài liệu Báo cáo khoa học: Oxidative stress in the hippocampus after pilocarpineinduced status epilepticus in Wistar rats doc
Ngày tải lên :
19/02/2014, 16:20
... oxidative stress.
Our findings show that pilocarpine induces SE,
which can produce alterations in superoxide dismutase
and catalase activities in different areas, thereby pro-
tecting the brain ... Interstrain differences in cognitive functions in
rats in relation to status epilepticus. Behav Brain Res
112, 77–83.
3 Treiman DM (1955) Electroclinical features of status
epilepticus. J Clin ... 10 min. A
550
was meas-
ured using a microplate reader. Nitrite concentration was
determined from a standard nitrite curve generated using
NaNO
2
.
Determination of GSH
GSH in the pilocarpine...
- 6
- 480
- 0
Báo cáo khoa học: Calcium, mitochondria and oxidative stress in neuronal pathology Novel aspects of an enduring theme pdf
Ngày tải lên :
07/03/2014, 12:20
... calcium
transients during reversible brain ischemia. Exp Brain
Res 104, 462–466.
188 Silver IA & Erecinska M (1992) Ion homeostasis in rat
brain in vivo: intra- and extracellular [Ca
2+
] and [H
+
]in
the ... functions, in addition to
having supporting functions for oxidative defences [79],
involving the thioredoxin system [40]. Aconitase acts
also as an iron-responsive element binding protein, iso-
citrate ... iso-
citrate dehydrogenase is an RNA-binding protein, while
kgd2p is a mitochondrial DNA binding protein [81–84].
Mitochondria from different brain regions contain
different amounts of KGDHC [85,86],...
- 18
- 549
- 0
Báo cáo khoa học: Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde–alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica pdf
Ngày tải lên :
15/03/2014, 23:20
... indicating the presence of two catalytically
independent domains, the N-terminal domain, display-
ing ALDH activity, and the C-terminal domain, con-
taining an iron-binding domain, which is involved ... against oxidative stress
damage, have not been reported.
In vivo effects on glucose-fermenting enzymes
and fluxes under oxidative stress
Recently, we reported that amebas incubated for
30 min in ... bind
close to the Fe–S clusters, blocking the access of ROS.
In this regard, a preliminary docking analysis with
coenzymes in the 3D model of EhPFOR suggested that
the CoA-binding site was, indeed,...
- 14
- 420
- 0
Báo cáo khoa học: Fasting-induced oxidative stress in very long chain acyl-CoA dehydrogenase-deficient mice pdf
Ngày tải lên :
15/03/2014, 23:20
... mice. Fasting induced a significant increase in both
genotypes; however, this was more evident in the
VLCAD KO mice (Fig. 3A). Interestingly, the MCT
diet also induced AOX gene expression in WT mice.
After ... 4.4 Uặmg
)1
in WT mice and
91.55 8.5 Uặmg
)1
in VLCAD KO mice in the non-
fasted state. Interestingly, the MCT diet combined with
fasting significantly reduced GPX activity in WT mice,
from 70.95 ... observed in human VLCADD [5]. In fact, in
both mice and humans, clinical symptoms become
mainly evident as a consequence of triggers such
as fasting, resulting in the accumulation of long-chain
acylcarnitines,...
- 10
- 381
- 0
Báo cáo khoa học: Oxidative stress induces a reversible flux of cysteine from tissues to blood in vivo in the rat pptx
Ngày tải lên :
16/03/2014, 02:20
... ROS, as indicated by the lack of increase
in lipid peroxidation observed in in vitro and in vivo
experiments [21]. In previous studies [22,23], we
observed that a single intraperitoneal administration ... Propargyl-
glycine is an inhibitor of cystathionase, the enzyme
that forms cysteine from cystathionine in the TSP; in
this case, treatment should eliminate the supply of
Cys derived from methionine [28]. ... The paucity of information in
this field is due, at least in part, to the intrinsic diffi-
culty in creating reliable animal models of oxidative
stress. Acute carbon tetrachloride administration is
frequently...
- 13
- 510
- 0
Báo cáo Y học: The role of zinc in the methylation of the coenzyme M thiol group in methanol:coenzyme M methyltransferase from Methanosarcina barkeri New insights from X-ray absorption spectroscopy doc
Ngày tải lên :
17/03/2014, 23:20
... M. Mutations in
His237 or Cys239, which are proposed to be involved in
ligating zinc, resulted in an over 90% loss in enzyme activity
and in distinct changes in the zinc ligands. In the
His237 ... was
determined using the Bradford method and bovine serum
albumin as standard [26].
Determination of zinc content
The zinc content was in principle determined as described by
Zhou et al. [15]. The zinc ... upon coenzyme M
binding to MtaA the number of sulfur ligands increased
from 1 to 2. However, this increase could also be due to the
binding of a cysteine residue to the active site zinc upon
enzyme–substrate...
- 7
- 464
- 0
Báo cáo khoa học: A nonribosomal peptide synthetase (Pes1) confers protection against oxidative stress in Aspergillus fumigatus ppt
Ngày tải lên :
30/03/2014, 10:20
... to
applied ROS. Interestingly, upregulation of pes1
expression was not observed following H
2
O
2
-induced
oxidative stress in cultures of A. fumigatus 293.1 grown
in either Sabouraud or 5% FBS in MEM ... Infect Dis
21, 161–172.
4 Denning DW (1996) Therapeutic outcome in invasive
aspergillosis. Clin Infect Dis 23, 608–615.
5 Denning DW (1998) Invasive aspergillosis. Clin Infect
Dis 26, 781–805.
6 ... domain organization from the standard (CAT)
n
architecture. NRP synthetases that fall into this group
include a peptide synthetase involved in biosynthesis of
the siderophore yersiniabactin from...
- 16
- 361
- 0
Báo cáo hóa học: " Oxidative stress in NSC-741909-induced apoptosis of cancer cells" potx
Ngày tải lên :
18/06/2014, 16:20
... several LOX inhibitors, including aesculetin (a
nonselective LOX inhibitor), MK886 (an inhibitor of the
5-LOX-activating protein), zileuton (a 5-LOX inhibitor),
baicalein (a 12/15-LOX inhibitor), ... apoptosis. In fact, ROS-induced oxidative stress
and cell death play important roles in the efficacy of many
antineoplastic agents [13,14].
To investigate whether oxidative stress is involved in
the ... National Cancer Institute. Using a reverse-phase pro-
tein microarray assay, we determined molecular changes
in 77 protein biomarkers in an oncrasin-sensitive lung
cancer cell line after treatment...
- 10
- 576
- 0
The genius in all of us new insights in david shenk
Ngày tải lên :
27/07/2014, 14:02
... heavily reinforced by the extraordinary coverage of reunited
identical twins—beginning in modern times with the amazing Jim twins.
In February 1979, in southwestern Ohio, a thirty-nine-year-old ... physical
changes (occurring in direct response to practice) in the muscles, nerves, hearts,
lungs, and brains of those showing profound increases in skill level in any domain.
2. Skills are specic. Individuals ... fare in the future, compared to others.
But measuring achievement was enormously dierent from pinpointing individual
capacity. Predicting how most kids will do is entirely dierent from declaring...
- 239
- 919
- 0
Báo cáo khoa học: "Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells" pptx
Ngày tải lên :
07/08/2014, 17:22
... (Gibco) containing 2 mM L-glutamine
supplemented with 0.01 mg/ml bovine pituitary extract
(Gibco, USA), 0.005 mg/ml insulin (Sigma, USA), 2.5 ng/ml
IGF (Sigma), 0.025 mg/ml trasferrin (Sigma, USA), ... study,
PM2.5 induced more MDA formation than PM10
suggesting that PM2.5 induced more oxidative stress than
PM10. In the single strand breakage assay using pBR322
DNA, the ratio of changed form II/total ... protein in the PM2.5
-
and PM10
-
t
reated rat lung epithelial cell was determined by Weste
rn
B
lotting. The lower shows the ratio of Nrf2/actin usi
ng
c
alculatging by densitometor. In the...
- 8
- 442
- 1
Báo cáo lâm nghiệp: "Response to an ozone gradient of growth and enzymes implicated in tolerance to oxidative stress in Acer saccharum (Marsh.) seedlings" pot
Ngày tải lên :
08/08/2014, 00:22
... biomass. Increasing oxidative stress limited new car-
bon skeletons production, less of which were then available
for the development of new sink tissues. The root/shoot ratio
was not affected by increasing ... CO
2
by PEPC increased in re-
sponse to O
3
in both groups of seedlings (Figs. 5E–5H) dur-
ing the growing season. The level of activity decreased in
the neoformed flush of the episodic seedlings when ... from each ush of each seedling, cut into 2 ì 2 mm pieces and
incubated in 5 mL of 100 mM phosphate buffer (pH 7.5) containing
40 mM KNO
3
and 1.5% 1-propanol. Each sample was vortexed for
2 min...
- 11
- 392
- 0
