oryzae avrxa21 activity is dependent on a type one secretion system
Characterization of a type III secretion system and other virulence associated genes in aeromonas hydrophila
... expression of flaA, flaB, lafA1 and lafA2 106 Fig IV.7 Comparative extracellular proteome analysis of AH-1S, ΔserA, ΔmepA and ΔserAΔmepA mutants 107 Fig IV.8 Comparative extracellular proteome analysis ... include the genus Aeromonas in the new family Aeromonodaceae based on 16S rRNA cataloguing, 5S rRNA sequencing and RNA-DNA hybridization data (MacDonell and Colwell, 1985; MacDonell et al., 1986) Two ... 1979) I.2.2 A hydrophila infections in humans Five Aeromonas species have been established as human pathogens: A hydrophila, A caviae, A veronii, A jandaei and A schubertii (Janda and Abbott, 1998)...
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Báo cáo khoa học: Type I receptor binding of bone morphogenetic protein 6 is dependent on N-glycosylation of the ligand pdf
... hindrance, whereas the canonical loop conformation (BMP-2-like conformation) could form similar noncovalent interactions with the type I receptor (Fig 2) Although the noncanonical loop conformation ... association rates (kon · 104ÆM)1Æs)1) of the BMP-2–BMPR-IA and BMPR-IB : interaction In contrast, association, and especially dissociation, for interaction of BMPs with ActR-I and ActR-II are ... prechordal mesoderm Cell 90, 257–269 Tomizawa K, Matsui H, Kondo E, Miyamoto K, Tokuda M, Itano T, Nagahata S, Akagi T & Hatase O (1995) Developmental alteration and neuron-specific expression of bone...
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Báo cáo khóa học: Hydrolytic cleavage by a group I intron ribozyme is dependent on RNA structures not important for splicing pot
... biochemical characterizations of the L9.2 mutants were performed, including hydrolysis at different mono- and divalent cation concentrations The corresponding 5¢-truncation constructs were analyzed at ... concentrations (5, 10 and 50 mM) and mM KCl A surprising observation was that the presence of 200 mM K+ (standard conditions) during the reaction has a negative effect on 3¢-SS hydrolysis Cleavage ... 3289–3302 25 Ikawa, Y., Naito, D., Aono, N., Shiraishi, H & Inoue, T (1999) Trans-activation of the Tetrahymena group I intron ribozyme via a non-native RNA–RNA interaction Nucleic Acids Res 27,...
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Báo cáo sinh học: "In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pot
... and 5’-GGA TCC TAG AAG GAG CTG GA-3’ for CCL7; 5’-CAG TCC ATG AGA AGG AGT CCA-3’ and 5’-AGA TCC TGC ACA GGA CTG TG-3’ for CCL8; 5’-AGG GCA TGG GTT TTA TTA TAT ATA TAT-3’ and 5’-TTT AAA AAT AAC ... GCA TGT GTA TAA AG-3’ and 5’-ATA GGT ATC CTG AAT AAA TGA GAA C-3’ for CXCL7; 5’-CAT GCT GGT GAG CCA AGC AGT TTG AA-3’ and 5’-CAC TTC TGT GGG GTG TTG GGG ACA AG-3’ for CXCL9; 5’- CGA TGC CTA AAT ... Ramana Davuluri for statistical data analyses This work is supported by National Institute of Health grants CA74294, and CA10815, by a grant from Corixa Corporation, by Intramural National Cancer...
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báo cáo hóa học:" In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pptx
... and 5’-GGA TCC TAG AAG GAG CTG GA-3’ for CCL7; 5’-CAG TCC ATG AGA AGG AGT CCA-3’ and 5’-AGA TCC TGC ACA GGA CTG TG-3’ for CCL8; 5’-AGG GCA TGG GTT TTA TTA TAT ATA TAT-3’ and 5’-TTT AAA AAT AAC ... GCA TGT GTA TAA AG-3’ and 5’-ATA GGT ATC CTG AAT AAA TGA GAA C-3’ for CXCL7; 5’-CAT GCT GGT GAG CCA AGC AGT TTG AA-3’ and 5’-CAC TTC TGT GGG GTG TTG GGG ACA AG-3’ for CXCL9; 5’- CGA TGC CTA AAT ... Ramana Davuluri for statistical data analyses This work is supported by National Institute of Health grants CA74294, and CA10815, by a grant from Corixa Corporation, by Intramural National Cancer...
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Báo cáo y học: "Effect of a short-term HAART on SIV load in macaque tissues is dependent on time of initiation and antiviral diffusion" pot
... untreated animals at the same stage of infection were used as controls (chronic untreated) Among the HAART treated animals, one (#20929) had undetectable PVL at the initiation of therapy (day 0), ... viral load as well as SIV-RNA, total SIV-DNA and 2LTR SIV-DNA were compared in placebo and HAART-treated macaques using a nonparametric Mann-Whitney test Differences in 3TC and IDV concentration ... tissues were assessed by the same test The correlation between 3TC concentration and residual viral replication/load in tissue, were evaluated using a nonparametric Spearman correlation test Acknowledgements...
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Báo cáo sinh học: "Tissue distribution of a plasmid DNA encoding Hsp65 gene is dependent on the dose administered through intramuscular delivery" docx
... suggests that some extrachromosomal plasmid DNA was maintained for at least months Southern blot analysis was done with liver and muscle (data not shown) samples at 30 days after immunization due ... tissue distribution of naked plasmid DNA-Hsp65 vaccine and RNA expression by months following intramuscular administration in mice Additionally, we investigated whether the plasmid DNA replicates ... DNA vaccination Nature 1999, 400:269-271 Bonato VL, Lima VM, Tascon RE, et al.: Identification and characterization of protective T cells in HSP65 DNA-vaccinated and Mycobacterium tuberculosis-infected...
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Báo cáo khoa học: Secretion of macrophage urokinase plasminogen activator is dependent on proteoglycans potx
... notion that uPA secretion is a characteristic feature of activated macrophages [25] Additionally, secretion of proteoglycans in monocytes and macrophages increases when the cells are activated ... the action of uPA, can cleave matrix proteins such as fibronectin, laminin and aggrecan, and also activate matrix- and membrane associated MMPs, fibroblast growth factor and transforming growth factor ... extracellular uPA was caused by post-translational mechanisms However, we not know at which level uPA is dependent on proteoglycans One possibility is that uPA is dependent on proteoglycans after...
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Báo cáo khoa học: A pH-dependent conformational change in EspA, a component of the Escherichia coli O157:H7 type III secretion system potx
... pedestal formation Cell Microbiol 3, 745–751 Vlademir VC, Takahashi A, Yanagihara I, Akeda Y, Imura K, Kodama T, Kono G, Sato Y, Iida T & Honda T (2002) Cortactin is necessary for F-actin accumulation ... intermediate structure In an attempt to understand how pH and urea concentration affect the conformations of EspA, we constructed an EspA pseudo phase diagram with urea concentration as a function ... pH -Dependent EspA conformational change molecules, which assemble to form flagella filaments [20] The EspA-containing filamentous apparatus may form a conduit for translocation of bacterial proteins...
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Báo cáo sinh học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein" pot
... 700 amino acid position B C1 a d a d C2 linker a d a d a d a d a d a MARV NP V Y QL A A KL RR LA L RE L AA I IV 315GVNVGEQYQQLREAAHDAEVKLQRRHEHQEIQAIAEDDEERKILEQFHLQKTEITHSQTLAVLSQKREKLARLAAEIENNIVEDQG400 ... Coomassie blue staining (to visualize the bacterially expressed proteins) and quantification of radioactive signals using BioImage analyzer BAS-1000 and the software TINA version 2.0, and Basreader ... of the plasmids was confirmed by automated DNA sequencing Detailed descriptions of cloning strategies are available on request Glutathione S tranferase (GST) pull-down assay pGex-NP and pGex-VP35...
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Báo cáo hóa học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein" pdf
... 700 amino acid position B C1 a d a d C2 linker a d a d a d a d a d a MARV NP V Y QL A A KL RR LA L RE L AA I IV 315GVNVGEQYQQLREAAHDAEVKLQRRHEHQEIQAIAEDDEERKILEQFHLQKTEITHSQTLAVLSQKREKLARLAAEIENNIVEDQG400 ... Coomassie blue staining (to visualize the bacterially expressed proteins) and quantification of radioactive signals using BioImage analyzer BAS-1000 and the software TINA version 2.0, and Basreader ... of the plasmids was confirmed by automated DNA sequencing Detailed descriptions of cloning strategies are available on request Glutathione S tranferase (GST) pull-down assay pGex-NP and pGex-VP35...
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Báo cáo khoa học: "Tumor response to radiotherapy is dependent on genotype-associated mechanisms in vitro and in vivo" ppsx
... values of r for any cell line, particularly for larger single fractions If this is a characteristic of all radioresistant glioblastoma lines, it not only suggests a rationale for large-fraction ... this variation based on genotype and fraction-size has a range of approximately 14, a remarkable difference for protocols that deliver the same total dose The schema at the 1) We have established ... xenografted human gliomas after fractionated radiotherapy Strahlenther Onkol 1989, 165(7):516-7 10 Rofstad EK: Fractionation sensitivity (alpha/beta ratio) of human melanoma xenografts Radiother Oncol...
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Báo cáo y học: " The pattern of methacholine responsiveness in mice is dependent on antigen challenge dose" ppt
... Kamelija, Turato Graziella, Ligabue Guido, Ciaccia Adalberto, Saetta Marina, Papi Alberto: Differences in airway inflammation in patients with fixed airflow obstruction due to asthma or chronic ... Johnson Malcolm, Vignola Anotonio M.: Asthma: from bronchoconstriction to airways inflammation and remodeling American Journal of Respiratory and Critical Care Medicine 2000, 161:1720-1745 Trautmann ... function techniques that are capable of assessing airway and parenchymal mechanics separately Total serum IgE was significantly elevated following systemic sensitisation but was not increased by aerosol...
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Báo cáo y học: "Intracellular interactions between APOBEC3G, RNA, and HIV-1 Gag: APOBEC3G multimerization is dependent on its association with RNA" potx
... assembly of A3 G multimers, A3 G-Gag interactions, and virion incorporation of A3 G Conclusion We have developed a robust BiFC assay to analyze intracellular A3 G -A3 G, A3 G-Gag, and A3 G-RNA interactions ... RNA, head-to-head and headto-tail dimers may also form A3 G multimerization is dependent on RNA binding Our results showed that A3 G multimerization was dependent on an intact CD1 while mutations ... polyclonal antibody (Sigma), or a rabbit anti-c-Myc monoclonal antibody (Sigma) at a 1:5,000 dilution, followed by a HRP-labeled goat anti-rabbit secondary antibody (Sigma) at a 1:10,000 dilution...
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THE INFLUENCE OF THE KU80 CARBOXY-TERMINUS ON ACTIVATION OF THE DNA-DEPENDENT PROTEIN KINASE AND DNA REPAIR IS DEPENDENT ON THE STRUCTURE OF DNA COFACTORS Derek S.
... GAG ATG CTG ATG ATA AAC ACA AGC TTA TAA CCA AAA CAG AGG CAA AAC AAG AAT ATC TTC TGA AAG ACT GTG ATT TAG AAA AAA GAG AGC CAC CTC TTA AAT TTA TTG TGA AGA AGA ATC CAC ATC ATT CAC AAT GGG GTG ATA ... TTA TAA CCA AAA CAG AGG CAA AAC AAG AAT ATC TTC TGA AAG ACT GTG ATT TAG AAA AAA GAG AGC CAC CTC TTA AAT TTA TTG TGA AGA AGA ATC CAC ATC ATT CAC AAT GGG GTG ATA TGA AAC TCT ACT TAA AGT TAC AGA ... TGA AAC TCT ACT TAA AGT TAC AGA TTG TGA AGA GGT CTC TTG AAG TTT GGG GTA GTC AAG AAG CAT TAG AAG AAG CAA AGG AAG TCC GAC AGG AAA ACC GAG AAA AAA TGA AAC AGA AGA AAT TTT AGT AGG GAT CCG TGG ATT...
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Báo cáo y học: "A2B adenosine receptor activity is reduced in neutrophils from patients with systemic sclerosis" ppt
... cross-react with A1 , A2 B, or A3 AR subtypes A2 B AR antibody is an affinity-purified rabbit polyclonal antibody raised against a region that corresponds to the second extracellular domain of A2 B AR ... Wellesley, MA, USA) To evaluate A2 B AR mediated cAMP accumulation, the reaction was carried out in the presence of selective A2 A antagonist SCH58261 at a concentration (100 nmol/l) able to block A2 A receptors ... reduction (by more than 50%) in NECA potency was observed, without any effect on agonist efficacy hypoxia, ischaemia and inflammation can stimulate adenosine production, A2 AR regulation has been...
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Structural and functional studies on type III and type VI secretion system proteins
... m) aa amino acid A/ E Attaching and effacing ATP adenosine triphosphate AU arbitrary unit AUC analytical ultracentrifugation BLAST Basic Local Alignment Search Tool BME β mercaptoethanol CCD charge ... (V.parahaemolyticus and V cholerae) YscN (ATPase), LcrH1 and-2 and SycE (chaperones), LcrE (structural “lid”) Environmental isolate, humans Aquatic isolate, humans Human pathogen Vibrio parahaemolyticus, ... 1993), Salmonella (Pang et al., 1995), E coli (Donnenberg and Kapper, 1992), Pseudomonas and various plant pathogens like Erwinia, Pseudomonas, Ralstonia, and Xanthomonas species (Bonas, 1994) A brief...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx
... cell adhesion, migration and intracellular signalling It was shown that purified CagL alone can directly trigger intracellular signalling pathways upon contact with mammalian cells and can even complement ... elongation by activating the Rap1 fi B-Raf fi Erk signalling cascade and by direct dephosphorylation and inactivation of focal adhesion kinase, FAK [7] Further studies have indicated that the CagAPY-induced ... phosphorylate injected CagA CagA can then modulate various signalling cascades associated with cell polarity, cell proliferation, actin-cytoskeletal rearrangements, cell elongation, disruption of...
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Tài liệu Báo cáo khoa học: Membrane targeting and pore formation by the type III secretion system translocon pdf
... T, Shibagaki Y, Danbara H & Okada N (2010) Functional characterization of SsaE, a novel chaperone protein of the type III secretion system encoded by Salmonella pathogenicity island J Bacteriol ... Structural and biochemical characterization of SrcA, a multi-cargo type III secretion chaperone in Salmonella required for pathogenic association with a host PloS Pathog 6, e1000751 42 Miki T, Shibagaki ... Shigella and Salmonella uptake by macrophages in the early steps of invasion, and have the ability to induce membrane extensions (filopodia and lamellipodia) on macrophages [55,63] Specifically, IpaC...
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Báo cáo khoa học: Architecture of the Helicobacter pylori Cag-type IV secretion system pdf
... gene-pathogenicity island (cagPAI), a 40 kbp DNA fragment that is transmitted horizontally; see the accompanying review by Tegtmeyer et al [14 ] CagA is considered as a paradigm for bacterial carcinogenesis ... HP0525 ATPase activity and CagA transfer, a hypothesis supported by ATPase assays and in vivo observation of complex formation It is therefore likely that HP1451 acts as a negative regulator for ... action, epithelial cells will have some of their major functions disturbed, such as cell–cell adhesion, signalling, adherence and proliferation [17] CagA translocation and 1214 phosphorylation...
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