origins isolation and in vitro culture

báo cáo khoa học: " Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress" ppt

Ngày tải lên : 12/08/2014, 03:20

... the luc+ gene in lines 156, 49 and 111 In addition, T-DNA tandem repeats were identified in lines 156 and 49, and vector backbone sequences were integrated in lines 17, 156, 49 and 179 (data ... designed and constructed the tagging vector and participated in sequence analysis SW maintained cell suspension and tissue cultures, subcultured and regenerated transgenic colonies and participated in ... I in this line The real-time observations strongly indicate a developmental regulation of the tagged promoter(s) in this line In contrast, all other lines, including the positive control line...

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Báo cáo khoa học: "Cold storage of in vitro cultures of wild chestnut and oak" pdf

Ngày tải lên : 08/08/2014, 19:21

... INTRODUCTION Collections of seeds and clonal material are traditional ways of storing genetic resources Use of cold stored (0-10°C) in vitro cultures is a complementary method of maintaining ... were placed in the cold immediately after subculture; and 2) T = 10 in which the explants were placed in the cold 10 d after subculture During these 10 d the cultures were kept under standard growth ... oak and chesnut cultures in cold storage for at least year without subculturing When stored after 10 d of preculture in the growth room, the proliferation capacity of the cultures was retained In...

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Analysis, sequencing and in vitro expression of PCR products

Ngày tải lên : 25/10/2013, 22:20

... functional domain mapping of proteins where fragments that represent protein domains are expressed and functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction ... separation in thin-coated capillaries containing nonpolymerized gel matrices and laser detection systems The introduction and removal of polymer from the capillaries, plus loading and running samples and ... nonbiotinylated single strand Both the strands can be used as templates for DNA sequencing reactions The immobilized strand can be stored and reused for further reactions into solution and leaving...

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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-speciﬁc blood vascular and lymphatic endothelial cells of the mouse pdf

Ngày tải lên : 18/02/2014, 17:20

... phosphotyrosine (D) The uterine BECs and LECs formed tube-like structures Bars = 200 lm (E) SV40-ori-positive plasmids bearing GFP and drug-resistance genes were maintained in the uterine BECs and LECs ... solution containing 0.2% type IV collagenase or NaCl ⁄ Pi containing 0.1% trypsin for 30–60 at 37 °C After pipetting the solution containing digested tissues several times, the enzyme-containing buffer ... harboring a GFP-expressing cassette into tsA58T-expressing endothelial cells Transfection was performed using Lipofectamine LTX and pcDNAÔ6.2-GW ⁄ miR-neg control plasmid (Invitrogen) according...

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Báo cáo Y học: A Ca2+/CaM-dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter ppt

Ngày tải lên : 18/03/2014, 01:20

... protein kinases are involved in stress signaling pathways [29] and recent work points towards the involvement of Ca2+/CaM-dependent protein kinases in stress signaling [16], changes in the level of ... containing lg puriﬁed p40 (fraction A) and those containing lg of both p40 and p72 PsCCaMK kinase (fraction B) were used for in vitro phosphorylation in the absence or presence of Ca2+, CaM, kinase ... be an in vitro substrate for the PsCCaMK, the effect of phosphorylation on its DNAbinding property was tested The protein was in vitro phosphorylated using cold ATP and used for DNA binding studies...

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báo cáo hóa học: " Beyond platinum: synthesis, characterization, and in vitro toxicity of Cu(II)-releasing polymer nanoparticles for potential use as a drug delivery vector" ppt

Ngày tải lên : 21/06/2014, 02:20

... synthesis, and metal loading RP conceived of the study, and participated in its design and coordination, carried out in vitro toxicity studies, SEM and EDX analysis and drafted the manuscript Competing ... the original loading solution determined Cu loading, resulting in values ranging between 12 and 16 wt.% based on these reactions conditions XPS was used to further confirm Cu loading and to probe ... synthesis, characterization, and metal binding properties of our Cu-binding particles, as well as preliminary in vitro toxicity in cancer cells Page of 10 Results Synthesis and characterization of...

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Báo cáo lâm nghiệp: "Eﬃcient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations" pptx

Ngày tải lên : 07/08/2014, 16:20

... putrescine treatments on in vitro rooting and on endogenous polyamine changes in difficult-to-root woody species, Sci Hortic 53 (1993) 63–72 [22] Rugini E., Involvement of polyamines in auxin and ... polyamines to increase rooting and to improve root quality, was first demonstrated in olive When the polyamines were combined with auxins, it promoted early rooting and increased the final rooting ... most of the tissue culture laboratories that produce teak plants in Indonesia and in the world, rooting is done ex vitrum due to the low frequency of in vitro rooting, resulting in a too long procedure...

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Báo cáo khoa học: " Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos" pps

Ngày tải lên : 07/08/2014, 20:23

... nisalahcotyc lm/gµ gniniatnoc pord muidem noitalupinam a otni derrefsnart dna ,muidem noitalupinam hserf ni eciwt dehsaw ,)24333 tshceoH lm/gµ gniniatnoc ASB-HLT eerf-muiclac( muidem noitalupinam ni nim ... dehsaw ,h rof edimixeholcyc lm /gµ 01 gniniatnoc muidem CVI ni detabucni erew setycoo detalumits-ortcelE 2lCgM Mm 50.0 dna 2lCaC Mm 10.0 gniniatnoc lotinnam Mm 082 ni ces yb detarapes cesµ 06 ... gip fo egavaelc rewol yltnacifingis dewohs EM-β gniniatnoc muidem denifed a ni derutam setycoo taht gnidnif eht derorrim hcihw ,egavaelc oyrbme desaerced yltnacifingis muidem noitarutam eht ot...

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Báo cáo lâm nghiệp: "Internal levels of plant growth regulators during in vitro culture of wild cherry (Prunus avium L.)" doc

Ngày tải lên : 09/08/2014, 04:20

... Epstein and Lavee (1986) reported a transformation of IBA into IAA during in vitro culture of Vitis vinifera and Olea europea The chemical pathway could be a 0-oxidation of the butyric side chain, ... horticoles et culture in vitro Rev For Fr 38, 60-68 Epstein E & Lav6e S (1986) Conversion of indole-3-butyric acid to indole-3-acetic acid by cuttings of gravepine (Vitis vinifera) and olive (Olea ... present in the culture medium (with or without IBA), no natural cytokinins could be detected in the explants, whereas each time BA was removed from the culture medium, natural cytokinins could...

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báo cáo khoa học: " A pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas in vivo and in vitro" pptx

Ngày tải lên : 10/08/2014, 10:21

... cell line in vivo and in vitro Based on these results, we hypothesized that the two different tumor cell lines, including GBC-SD and SGC-996, can exhibit significant different invasive ability and ... seeded onto the gels and incubated at 37°C with 5% carbon dioxide and humidity The cultures were maintained in DMEM supplemented with 10% FBS and 0.1% gentamicin sulfate The culture medium was ... staining: 12 paraffin-embedded tissue specimens of tumor xenografts were deparaffinized, hydrated, and stained with H&E Companion serial section were stained with double staining of CD31 and...

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Báo cáo y học: "Phenotype and in vitro function of mature MDDC generated from cryopreserved PBMC of cancer patients are equivalent to those from healthy donors" ppt

Ngày tải lên : 11/08/2014, 10:23

... cytokine repertoire of DC matured in the presence of inflammatory stimuli comprises pro-inflammatory cytokines and chemokines, including the T cell inhibitory cytokine IL-10, the Th-1 promoting ... PerCP-Cy5.5 and CD4 APC and acquired on a flow cytometer CD3+CD4+ lymphocytes were gated including the blasts and excluding CD209+ MDDC The percentage of cells showing decreased CFSE staining intensity ... cells, excluding the CD209+ MDDC (stimulator cells), with decreased CFSE staining intensity resulting from dilution during cell division (viz., the fluorescence intensity of membrane staining halves...

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Báo cáo khoa học: "In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair" ppt

Ngày tải lên : 12/08/2014, 18:22

... Veterinaria Scandinavica 2009, 51:12 http://www.actavetscand.com/content/51/1/12 Background Materials and methods The composition and structure of a membrane are important factors in determining ... Differences in the manufacturing according to initial concentrations of carbon sources, surface/volume ratios, strain of Acetobacter and extended times of fermentation interfere in the final product ... increasing interest because of their potential in tissue engineering applications [20] They can be obtained from adult bone marrow and are capable to differentiate into other phenotypes including the...

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Báo cáo y học: " Reduced CD4 T cell activation and in vitro susceptibility to HIV-1 infection in exposed uninfected Central Africans" pptx

Ngày tải lên : 13/08/2014, 09:20

... susceptibility to in vitro HIV-1 infection in EUs We evaluated PBMC susceptibility to in vitro infection using two HIV-1 strains with different tropisms, HIV-1 NL-4.3 (X4) and HIV-1 BaL (R5) In order ... donor used as standard in each assay and designated thereafter "standard PBMC" Viral inoculums able to consistently infect standard PBMCs were determined in preliminary assays For each individual, ... clearance by CAR Health Ministry and Health Authorities The political situation in CAR, including a coup in March 2003, caused movements of population including individuals enrolled in this study Consequently,...

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Báo cáo y học: "Data from necropsy studies and in vitro tissue studies lead to a model for allometric scaling of basal metabolic rate" pot

Ngày tải lên : 13/08/2014, 23:20

... respectively, and 26 µm and 28 µm in horses and cows, respectively [38], and the scaling exponent for thickness of this layer is approximately 0.09 Combining this exponent with an estimate of the scaling ... positive and strictly decreasing as y and M increase, the above derivative is strictly increasing The second prediction is that the above slope approaches BMRv = avM0.86, βn as M increases (and y increases) ... defined confidence interval for the scaling exponent Note that no information on metabolic rates in the studies of Heusner and Kleiber is used in generating the BMR predictions Discussion In...

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IN VITRO CULTURE OF PETIOLE LONGITUDINAL THIN CELL LAYER EXPLANTS OF VIETNAMESE GINSENG

Ngày tải lên : 20/04/2015, 21:33

... equally well–growing and healthy with leaves, shoots, and main and fiber roots Making a vertical cut down the in vitro leaf petiole was carried out in oder to have longitudinal thin cell layers ... shoot, callus and adventitious root production of Vietnamese ginseng, and TLC and HPLC fingerprint methods were utilized for investigating the saponin content biomass of calli MATERIALS AND METHODS ... combinatorial effect of 2,4-D and BA, and 2,4-D and TDZ, combination of NAA and BA gave the lower rate of callogenesis Thin cell layer systems could be used as a tool for in vitro regeneration and...

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In vivo and in vitro studies of th17 response to specific immunotherapy in house dust mite induced allergic rhinitis patients

Ngày tải lên : 23/09/2015, 14:47

... treatment were used in the following in vivo and in vitro studies 3.3 In vitro Study To mimic the functional response of Th17 and other Th cell subpopulations to HDM allergens, an in vitro allergen ... between in vivo and in vitro findings, confirming that not only Th2, but also Th17 cells play an important role in the context of allergic inflammation IL10 producing CD4+ T cells showed a distinct ... 20 minutes in dark Cells were then re-suspended and permeabilized in Permeabilization Buffer (eBioscience) to facilitate the intracellular staining PBMCs were stained with intracellular cytokines...

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In vivo and in vitro study on potential combination therapy of COL 3 and tamoxifen in breast cancer a pilot study

Ngày tải lên : 09/10/2015, 11:24

... PROTEIN BINDING STUDY 5.1.1 Introduction The binding of drugs to plasma and tissue proteins is a major determinant of drug distribution throughout the body Protein binding displacement interactions ... examine if the in vitro protein binding of COL-3 would be affected by tamoxifen 5.1.2 Materials And Methods The serum protein binding of COL-3 in the absence and presence of tamoxifen was determined ... and COL-3 bind mainly to albumin So protein-binding interaction, where competition for the protein-biding site involves, could be a good reason to explain this phenomenon Some cases of protein...

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In vitro culture of small floral receptacle of gerbera jamesonil

Ngày tải lên : 08/01/2017, 21:04

... e t io n ia t v d in f p la n ts T h e e s r o b t in du 20 m g BA in d e /L p la e T h th e m T h r o it x E t u e ll R u s in g t io n e ir r e s u e a w o o t o n ts fo in g o n r a ho w ... be u s d w e o n o it n a r a h i n in g v a r e r d d e d w i t h IB A K ey w o r d s G e r be a r aj e s o n m 欢迎订阅 P e r e a s i mg f ar 川 m ta e e le in g ia e x mo r /L e so n a s m d ho ... 苏林业科技》为公开发行的综合性的林业科学技术刊物结 n 。 t IV e 查 be r e e e io o de m y 5一 d w it h in e s o aj m r a ji e l G l : ( C m m F u j ia e r e a s e M S m e diu m le d G o u n de a e a...

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Báo cáo " Isomeranzin against Herpes simplex virus in vitro from Clausena heptaphylla (Roxb.) W. & ARN.: Isolation, structure and biological assay " potx

Ngày tải lên : 03/04/2014, 15:20

... compound in 10% ethanol) or ml of saline (for virus control and cell control) and given ml 2x MEM The cells were incubated for 72 hours and observed for cytopathic effects from the virus and cytotoxic ... cells were about 40% destroyed At this point in time viral growth had only been observed in cells treated with DMSO and infected with HSV-1 However, in other solvents with 1mg/ml compound, the ... ( -pyrone ring protons) The presence of a 3’methyl-2’-oxobutyl side - chain was indicated by the appearance of a two-protons singlet at 4.00 ppm, a one-proton septet at 2.83 ppm and doublet...

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Báo cáo khoa học: "Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System" ppsx

Ngày tải lên : 07/08/2014, 17:22

... 1995, 52, 1410-1417 10 Keskintepe, L and Brackett, B G in vitro developmental competence of in vitro- matured bovine oocytes fertilized and cultured in completely defined media Biol Reprod 1996, ... oocytes in a chemically defined, protein-free culture medium Biol Reprod 1993, 48, 1320-1325 12 Lee, E S and Fukui Y Synergistic effect of alanine and glycine on bovine embryos cultured in a chemically ... were to examine the effects of different protein sources supplemented in culture media on the in vitro development of bovine embryos, and to examine the effects of glucose, phosphate and citrate...

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