... cloned into pGEX-4T-2 The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions ofthe v-KIND KIND2 andthe MAP2 CD were ... structural and functional importance ofthe v-KIND and MAP2 interaction core modules in the regulation of dendrite growth and branching in hippocampal neurons and cerebellar granule cells Further studies ... proteins of these subregions (Fig S1C) and 1654 Fig Of all members ofthe KIND family of proteins, only KIND2 binds to MAP2 Pull-down assay (PD) ofthe endogenous MAP2 from cerebellar lysates of P21...
... for the determination ofthe structures ofthe conformers ofthe C-domain The dipolar couplings provided long-distance information on the global folding of both conformers Thestructureofthe ... on one side ofthe b-sheet and two on the other side The rmsd ofthe heavy atoms (Ca, C, and N) ofthe protein core, when the NMR structures of both conformers are superimposed on the crystal ... between the signal ofthe HN of Gly337 in the open conformer andthe signal ofthe water protons All of these observations indicate that the loop region ofthe open conformer has a higher degree of...
... comparison ofthe effects of rough and smooth LPSs of E coli, both in the presence and absence of EDTA (Fig 1) The rate of oxidation was increased in the presence ofthe LPSs of both the smooth and rough ... in binding ofthe two types of LPSs The effect ofthe LPSs on the rate of oxidation of Hb A0 was much less than on cross-linked Hb and furthermore, the differences in structures ofthe LPSs were ... effects ofthe LPSs of rough and smooth E coli on the oxidation of Hb A0, both in the presence and absence of EDTA, is provided in Fig The rate of oxidation was increased in the presence ofthe LPSs...
... in the mutant First, there is an increase in the negative charge in the active site and, second, breaking the salt bridge would alter the quaternary structureofthe protein and therefore the ... in the ArCMLE1 structureThe figure was created from the coordinates ofthe P212121 structureof ArCMLE1 andof PpCMLE (Protein Data Bank code 1RE5) (B) Water cavity below the active site The ... structureThe variation in size andthe observed amino acid modification between PpCMLE and ArCMLE suggested important differences between the two enzymes Therefore, the crystal structureof the...
... STRUCTUREANDFUNCTIONOFTHE ARABIC VERB StructureandFunctionofthe Arabic Verb is a corpus-based study that unveils the morpho-syntax andthe semantics ofthe Arabic verb ... very indicative, in our opinion, ofthe adequacy andthe basis of this orientation, on the one hand, andthe destiny of linguistics, on the other hand Of course, these implications might be too ... constructions The frequency of WA, FA-, LA-, and Ø in front of QAD Frequency ofthe compound perfect The distribution and frequency of sawfa/saThe frequency of QAD, laʕalla, and rubbamaa The contextual...
... to test the factor stability We posited that the factor pattern ofthe HADS was invariant between the outpatient and student groups and between the men and women This is on the basis ofthe previous ... are ofthe same quality in terms ofthe components, andthe difference between the two groups is found in terms of illness severity [25] It remains unclear whether this is true for the HADS Therefore ... that ofthe HADS-A in this study Therefore, it should be noted that the validity and reliability ofthe HADS-D subscale is inferior to the HADS-A subscale in the current Japanese version of the...
... dimensionality ofthe HADS andthe behaviour of individual items The results ofthe current study generally provide support for the bi-dimensionality ofthe HADS, however the content ofthe factors ... model, due to the small item pool, the emphasis of anhedonia items in the depression subscale, andthe lack of somatic items The clinical utility of a three subscale structureofthe HADS is also ... 56) Overall these results support the bi-dimensionality ofthe HADS, however the content ofthe factors obtained not fully support the original scale structure proposed by the authors The major...
... determined the genomic sequences ofthe ARV L2 genome segments from two different strains of ARV (ARV138 and ARV176) in order to expand the available ARV sequence database, determine sequences ofthe ... help tether the RdRp to the underside ofthe core shell This hypothesis could be tested by extending such ARV:MRV:AqRV sequence comparisons to the other core proteins In conclusion, we report the ... Background The avian reoviruses (ARVs) are members ofthe family Reoviridae, the only group of dsRNA viruses (out of seven dsRNA virus families) that infect mammals [1,2] The ARVs are the prototypic...
... explores the linkage between organizationalstructureand cross-cultural management against the background ofthe Credit Suisse experience It is the contention of this paper that the type ofstructure ... President ofthe corporation The German students recommended that there should be greater clarity regarding the roles, responsibilities, and spheres of activity ofthe two department heads These roles, ... Project Copernicus The hypothesis that emerges here is: While traditional definitions oforganizationalstructure may apply in situations where the nature ofthe work is routine andthe workforce...
... J(0)] The dashed curve (theoretical curve) was calculated for J(0) and J(xN) as a functionof s, using a simple Lorentzian functionThe left-hand inset shows an overview ofthe theoretical curve and ... Fig The localization ofthe experimental points in Fig along the correlation line is directly related to the distribution ofthe energy between the overall tumbling andthe internal mobility, and ... residues and hence does not conflict with the global interpretation ofthe data With the exclusion ofthe N-terminal octapeptidyl and C-terminal nonapeptidyl fragments of Glu17, Leu34 and Lys46, the...
... markers are indicated on the left of Spatzle-C108 (Fig 8A) Analysis of tryptic peptides ¨ from these bands by MS ⁄ MS and mascot software identified them as attacin-1, lysozyme, and cecropinA ⁄ B (Table ... yet in other insect species Although it is clear that Drosophila Spatzle acts as ¨ the ligand ofthe Toll receptor in two important physiological pathways [16,36], the functions of other homologs, ... comparison ofthe genomic and cDNA sequences Analysis ofthe amino acid sequences deduced 158 from the cDNA, including prediction of signal peptide and glycosylation sites, was carried out in the expasy...
... between the HADS anxiety domain andthe CRQ emotional functionand mastery domains and between the HADS total score andthe CRQ emotional functionand total score None ofthe correlations between the ... participated in the design ofthe study, performed the statistical analysis and drafted the manuscript MF participated in the design ofthe study, collection ofthe data and revised the manuscript ... follow-up for HADS, CRQ and Feeling Thermometer scores andthe correlations between outcomes The change scores for the CRQ and Feeling Thermometer both exceeded the threshold of their minimal important...
... participated in the design ofthe study and assisted in the drafting ofthe manuscript VL participated in the design ofthe study and assisted in the drafting ofthe manuscript CM participated in the design ... underlying factor structureofthe HADS The HADS clearly cannot be both bi-dimensional and tri-dimensional within the same data set and further clarification ofthestructureofthe instrument is ... for screening and case detection utility and accuracy [16] The present study was designed to examine the underlying factor structureofthe Chinese version ofthe HADS in a mainland Mandarin-speaking...
... breathing A high level of postoperative pain is common because ofthe cutting ofthe skin, muscles and pleura as well as the retraction of muscles and ligaments, pleural and septal nerve irritation ... between the pericardium andthe mediastinal pleura, andthe left phrenic nerve descends between the subclavian artery and left common carotid lateral to the vagus nerve and anterior to the left ... conductance due to the freezing ofthe myocardium in the preoperative period for myocardial preservation [17,22], reduced perfusion ofthe phrenic nerve due to injury ofthe branches ofthe internal...
... [26] The variability ofthe results of Rasch analyses across a range of diseases suggests that the performance ofthe HADS may vary by diagnostic group and reinforces the need for clinicians and ... ofthe scale caused by removing the effects of local dependency in the data through the testlet design The performance ofthe HADS has been called into question following Rasch analysis in other ... test These estimates are then compared andthe number of significant t-tests outside the ± 1.96 range indicates whether the scale is unidimensional or not Generally, where less than 5% of the...
... cloning ofthe entire genome in BAC and transfecting the BAC-contained viral cDNA under the control of a CMV promoter [37] The use of BAC DNA provides the remarkable benefit of being able to handle ... 7b-dependent extension ofthe replication-attenuating effect of interferon However, the additional extent of attenuation on top ofthe effect of interferon beta was ofthe same size as that observed ... than those from the cell culture isolate, indicating absence ofthe deletion in the patient Of note, clinical samples from the wife ofthe index patient, who got infected by her husband in later...
... cloning ofthe entire genome in BAC and transfecting the BAC-contained viral cDNA under the control of a CMV promoter [37] The use of BAC DNA provides the remarkable benefit of being able to handle ... 7b-dependent extension ofthe replication-attenuating effect of interferon However, the additional extent of attenuation on top ofthe effect of interferon beta was ofthe same size as that observed ... than those from the cell culture isolate, indicating absence ofthe deletion in the patient Of note, clinical samples from the wife ofthe index patient, who got infected by her husband in later...
... by the weight ofthe abdominal content, and increases the pulmonary blood volume in the dependent parts ofthe lungs under the effect of both gravity andthe increase in cardiac output [7] The ... alterations ofthe cardiovascular function [10] Another characteristic ofthe patients with end-stage heart disease is represented by the higher values of PaO2 and SaO2 reached at the five times ofthe ... Monitoring ofthe patients The main data on the general characteristics ofthe patients (age, weight, preoperative LVEF, preoperative therapy) are reported in Table 1; the times ofthe headdown...
... R-J and MW collected the clinical data and drafted the manuscript CK analyzed the data and wrote the manuscript The consequence of our findings was the interposition of carbon filters in the ... days when thehospital water supply was disinfected During dialysis, 706 arterial blood gas analyses were carried out, 89 of them on the days of water disinfection The results ofthe methemoglobin ... synchronize the exact time courses of hydrogen peroxide concentration in thehospital water supply and methemoglobinemia on the days of disinfection retrospectively Due to the retrospective nature of...
... expressed ones [5-7] We have studied the relationship between the phyletic age of a protein and its expression profile, and related this to thefunctionofthe protein The term 'phyletic age' used here ... proteinsthe inanalysis analysis tionalhere assignments of analysis in the analysis analysis FuncFunctional File usedof of thethe proteins analysis analysis Phyletic Additionalforof ofthe proteins ... table andthe test statistics of these were used to form an estimate of their distribution under the null hypothesis, to which the observed test statistic can be compared Additional data files The...