or tlck treated jtag cells
báo cáo hóa học:"Transplantation of selected or transgenic blood stem cells – a future treatment for HIV/AIDS?" pot
... require an expansion or the donor pool by a factor of at least 10 However, this problem is alleviated by the worldwide cooperation of stem cell registries in the Bone Marrow Donors Worldwide and European ... typing information to narrow down the set of potential donors for a given patient, so these http://www.jiasociety.org/content/12/1/10 individuals could be tested for CCR5-delta32 before costly ... biosafety reduced S3 laboratory environment for the transgene introduction Future gene therapy approaches may involve allogeneic transplants, obviating the need for laboratory conditions of high...
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Báo cáo sinh học: "Transplanted astrocytes derived from BMP- or CNTF-treated glialrestricted precursors have opposite effects on recovery and allodynia after spinal cord injury" docx
... reasons for considering the use of non-CNS cells such as Schwann cells, olfactory ensheathing cells and marrow stromal cells for CNS repair has been their relative ease of isolation compared to cells ... spinal cord injury being one of the primary targets for early translation of laboratory efforts to clinical trials A variety of cell types of both non-CNS and CNS origin, such as Schwann cells ... of CGRP+ immunoreactivity within lamina III of the dorsal horn of (d) GDACNTF- and (e) GRP -treated spinal cords compared to (b) control injured and (e) GDABMP- treated spinal cords Scale bar...
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Role of SPHK2S1P signalling in regulating mitochondrial function in the MPTP induced mouse model of parkinsons disease and in the MPP treated MN9D cells
... S1P1 receptor was higher in the healthy neurons of the SNc Blocking of the receptor using a commercially available receptor antagonist significantly reduced the viability of the cells Moreover, ... 4′,6-diamidino-2-phenylindole DAT dopamine transporter DBS deep brain stimulation DCF 2′,7′-dichlorofluorescein DCFH 2',7'-dichlorodihydrofluorescin DDC dopa decarboxylase DHCER dihydroceramide ... derived growth factor PGC-1α Peroxisome proliferator-activated receptor-gamma coactivator PINK1 PTEN-induced putative kinase PTEN phosphatase and tensin homolog PVDF polyvinylidenedifluoride RNA ribonucleic...
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2D DIGE analysis of butyrate treated hepg2 cells
... changed to fresh cDMEM for control cells or cDMEM containing mM sodium butyrate for treatment cells Both control and treated cells were maintained under similar conditions The cells were harvested ... polyvinylidene difluoride membrane (Bio-Rad Laboratories) Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad Laboratories) Trans-Blot Cell (Bio-Rad Laboratories) 3.1.3.8 Scanners UMAX ImageScanner ... Dithiothreitol (DTT) Bio-Rad Laboratories DNase I Roche Diagnostic (Mannheim, Germany) EDTA disodium dihydrate Bio-Rad Laboratories Formaldehyde Merck Glycerol BDH laboratory Supplies (Poole, England)...
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Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine fibrosarcoma L929 cells doc
... (A-a, h for oridonin; A-b,c, 24 h for oridonin) Scale bar = lm (A-a,b) and 0.5 lm (A-c) The cells were cultured with 50 lM oridonin for h (B-a), h (B-b), 12 h (B-c), 24 h (B-d) or 36 h (B-e), or co-incubated ... Fig Oridonin-induced MMP collapse and cell apoptosis were rescued by NAC in L929 cells The cells were incubated with 50 lM oridonin for or 24 h, or co-incubated with mM NAC for 24 h The cells ... 4) **P < 0.01 The cells were transfected with p38 or control siRNA for 24 h, followed by stimulation with oridonin for 24 h The MDC fluorescent intensity of oridonin -treated cells was analyzed...
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Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
... incubated for h, as recommended by the manufacturer Transfected cells were cultured for 24 h in serum-free medium and incubated for 24 h in the same medium containing EPA, DHA or AA After incorporation ... and Raf mutants, and the effects of incorporated n-6 and n-3 PUFAs were explored in cells treated with IL-1b or 10% (v/v) fetal bovine serum (Fig 3) Ras K12 or Raf BXB (dominant-positive mutants) ... incorporation of n-3 PUFAs interferes with these signalling molecules The cyclin D1 promoter activity induced by Ras or Raf dominant-positive expression vectors is lower for n-3 PUFA -treated cells...
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Báo cáo khoa học: Are UV-induced nonculturable Escherichia coli K-12 cells alive or dead? ppt
... E coli cells alive or dead? (Eur J Biochem 270) 2693 • Fig Protein synthesis Protein synthesis of untreated cells ( ), UV1 -treated cells (j), UV2 -treated cells (m), detected by incorporation ... Biochem 270) Fig Visualization of cells Visualization by fluorescent in situ hybridization of untreated cells (A), UV1 -treated cells (B) and UV2 -treated cells (C) before (A1, B1, C1) and after DVC ... For untreated cells, glucose incorporation in the absence of catalase reached a steady state level of about 3.6 lmol [14C]glucose per lg protein after h of incubation UV 1treated cells incorporated...
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Báo cáo sinh học: "The bioenergetic signature of isogenic colon cancer cells predicts the cell death response to treatment with 3-bromopyruvate, iodoacetate or 5-fluorouracil" pdf
... cells and tumors [9,11] Moreover, according to large cohort studies of colon [10,19], lung [9,14] and breast [16,20] cancer patients, low tumor bioenergetic signatures are associated with poor ... for experiments Where indicated, cells were incubated with 10 μM 5-FU for 48h, or μM 3BrP or 100 μM IA for 7h Protein electrophoresis and Western blot analysis Cells were resuspended in lysis ... be significantly increased in 5-FU treated SM -cells (1.0 ± 0.2 vs 2.2 ± 0.1 a.u./ 15000 cells for control and 5-FU treated cells, respectively, P < 0.05) Therefore, efficient activation of apoptosis...
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báo cáo hóa học: " Correlations among improvements in urgency urinary incontinence, health-related quality of life, and perception of bladder-related problems in incontinent subjects with overactive bladder treated with tolterodine or placebo" docx
... showed similar correlations except for Emotions and Symptom Severity, which were poorly correlated Categorical improvement on the PPBC was correlated with improvements in KHQ domain scores in the ... scored on a scale from (best) to 100 (worst) The Symptom Severity scale is scored from (best) to 30 (worst) Decreases in KHQ domain scores indicate an improvement in HRQL The minimally important ... information regarding the patient's perspective on his or her symptoms or the impact of treatment Instruments that measure patient-reported outcomes (PROs) provide valuable additional information...
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báo cáo hóa học:" The bioenergetic signature of isogenic colon cancer cells predicts the cell death response to treatment with 3-bromopyruvate, iodoacetate or 5-fluorouracil" ppt
... cells and tumors [9,11] Moreover, according to large cohort studies of colon [10,19], lung [9,14] and breast [16,20] cancer patients, low tumor bioenergetic signatures are associated with poor ... for experiments Where indicated, cells were incubated with 10 μM 5-FU for 48h, or μM 3BrP or 100 μM IA for 7h Protein electrophoresis and Western blot analysis Cells were resuspended in lysis ... be significantly increased in 5-FU treated SM -cells (1.0 ± 0.2 vs 2.2 ± 0.1 a.u./ 15000 cells for control and 5-FU treated cells, respectively, P < 0.05) Therefore, efficient activation of apoptosis...
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báo cáo hóa học:" Gene Expression and Pathway Analysis of Ovarian Cancer Cells Selected for Resistance to Cisplatin, Paclitaxel, or Doxorubicin" docx
... cancer cells selected for resistance to cisplatin, paclitaxel, or doxorubicin Cheryl A Sherman-Baust1, Kevin G Becker2, William H.Wood, III2, Yongqing Zhang2, and Patrice J Morin1,3* Laboratory ... thank the members of our laboratory for useful comments on the manuscript We thank Dr Bingxue Yan for technical help on various aspects of this work This research was supported entirely by the Intramural ... doxorubicin or paclitaxel In contrast, the doxorubicinderived resistant cells (OV90D-6 and OV90D-7) exhibited significant cross-resistance to paclitaxel, and the paclitaxel-derived resistant cells...
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Báo cáo y học: "Primary Care Outcomes in Patients Treated by Nurse Practitioners or Physicians" docx
... scale or summary score at months This is true for both the unadjusted scores and scores adjusted for demographics and baseline health status The additional analysis (not shown) of the summary scores, ... in “overall satisfaction” or “communications” factors or in willingness to refer the clinic to others The difference in mean score for the “provider attributes” factor, however, was significant, ... 2011 PRIMARY CARE BY NURSE PRACTITIONERS OR PHYSICIANS (3.2 for general health; 3.3 for vitality; 3.4 for mental health; 3.4 for social function; and 4.2 for bodily pain) and less than points on...
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Báo cáo toán học: "Expression and function of micro RNAs in immune cells during normal or disease state" pptx
... downregulated in effector cells compare to naïve cells and increased back in memory T cells [14] Effector T cells originate from naïve T cells following antigen exposure and are considered cells in a high ... lineages, through progenitor cells For simplicity, the progenitor cells are not shown and only four of the main hematopoitic lineages are depicted miRNAs that are reported to be involved in the ... reported to be necessary for the differentiation and function of immune cells are found to be abnormally expressed in both solid and liquid tumors Thus the first miRNA microarray profiling reported...
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Báo cáo y học: "Brain choline concentrations may not be altered in euthymic bipolar disorder patients chronically treated with either lithium or sodium valproate" pps
... there was any personal history, or immediate family history, of psychiatric disorder For patients, diagnoses were made using DSM-IV criteria for Bipolar Disorder Type I or Type II following detailed ... brain choline concentration changes in bipolar disorder patients World J Biol Psychiat 2004, 5:35-41 Gordon RE, Ordidge RJ: Volume selection for high resolution NMR studies [abstract] Proc Soc ... primary correlate of Choline concentrations This result has been reported briefly in a previous publication [38] The mean Cho/Cr ratio with this measure was 1.46 ± 0.04 for controls, 1.18 ± 0.07 for...
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Báo cáo y học: "Serum cartilage oligomeric matrix protein (COMP) decreases in rheumatoid arthritis patients treated with infliximab or etanercept" pot
... the study Furthermore, no intra-articular glucocorticoid injec- tions were given during the study period or months prior to enrolment The reason for the rigorous control of glucocorticoid administration ... protocol and had been treated for at least months They all fulfilled the American College of Rheumatology criteria for RA [16] Only patients who were not receiving glucocorticoids or who were on a ... reported for the TNF-blockers could be corroborated by changing levels of a primarily cartilage-derived marker during treatment Materials and methods Patients R182 Patients with RA who are treated...
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Báo cáo y học: "Collagen type II (CII)-specific antibodies induce arthritis in the absence of T or B cells but the arthritis progression is enhanced by CII-reactive T cells" ppsx
... arthritis in mice deficient in B and /or T cells WT To understand the role of B and T cells in antibody-mediated inflammation, mice deficient in either B cells or T cells or both were injected with a ... B and T cells, heterozygous female mice R545 deficient in B cells and T cells were crossed with doubly deficient males, and offspring were investigated for the absence of B cells and T cells by ... were scored for arthritis up to month and were included in the calculations None of the control mice developed arthritis Mice deficient in B cells or T cells were compared with WT mice for statistical...
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Báo cáo y học: "Natural killer T cells and rheumatoid arthritis: friend or foe" ppt
... effect of Vαi NKT cells in CD1d–/– mice, while not affecting joint inflammation in wild-type animals Although several studies have highlighted important immunoregulatory properties for TGF-β1 in experimental ... negative regulator for TGF-β1 for many years, the role of IL-4 reported by Kim et al is unexpected and warrants further investigation [15] Likewise, the precise mechanism(s) by which Vαi NKT cells are ... KRN7000, a novel immunomodulator, and its antitumor activities Oncol Res 1995, 7:529-534 Hammond KJ, Kronenberg M: Natural Killer T cells: natural or unnatural regulators of autoimmunity? Curr Opin...
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Báo cáo y học: " Myeloid dendritic cells correlate with clinical response whereas plasmacytoid dendritic cells impact autoantibody development in rheumatoid arthritis patients treated with infliximab" pdf
... looked for a correlation between absolute counts of blood DCs and the clinical status or laboratory tests known to reflect disease activity (DAS28, Health Assessment Questionnaire score, and ... TNF/TNF-receptor system appears to play an important role in SLE pathogenesis, as is exemplified by TNFαinduced amelioration of murine lupus nephritis [17] and an increased soluble TNF-receptor correlation ... mononuclear cells (PBMCs) from healthy donors were cultured out in the presence of influenza virus (Flu) with or without infliximab or TNFα After 10 days, we analyzed by flow cytometry the proportion...
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